全文获取类型
收费全文 | 2392篇 |
免费 | 118篇 |
国内免费 | 19篇 |
专业分类
耳鼻咽喉 | 14篇 |
儿科学 | 19篇 |
妇产科学 | 23篇 |
基础医学 | 255篇 |
口腔科学 | 40篇 |
临床医学 | 205篇 |
内科学 | 581篇 |
皮肤病学 | 35篇 |
神经病学 | 205篇 |
特种医学 | 82篇 |
外科学 | 421篇 |
综合类 | 11篇 |
一般理论 | 1篇 |
预防医学 | 62篇 |
眼科学 | 29篇 |
药学 | 166篇 |
中国医学 | 2篇 |
肿瘤学 | 378篇 |
出版年
2024年 | 3篇 |
2023年 | 37篇 |
2022年 | 81篇 |
2021年 | 112篇 |
2020年 | 55篇 |
2019年 | 54篇 |
2018年 | 65篇 |
2017年 | 55篇 |
2016年 | 79篇 |
2015年 | 73篇 |
2014年 | 84篇 |
2013年 | 119篇 |
2012年 | 187篇 |
2011年 | 158篇 |
2010年 | 89篇 |
2009年 | 85篇 |
2008年 | 110篇 |
2007年 | 127篇 |
2006年 | 162篇 |
2005年 | 130篇 |
2004年 | 106篇 |
2003年 | 119篇 |
2002年 | 91篇 |
2001年 | 35篇 |
2000年 | 45篇 |
1999年 | 36篇 |
1998年 | 19篇 |
1997年 | 11篇 |
1996年 | 19篇 |
1995年 | 10篇 |
1994年 | 6篇 |
1993年 | 8篇 |
1992年 | 12篇 |
1991年 | 13篇 |
1990年 | 16篇 |
1989年 | 13篇 |
1988年 | 9篇 |
1987年 | 10篇 |
1986年 | 12篇 |
1985年 | 12篇 |
1984年 | 7篇 |
1981年 | 3篇 |
1979年 | 4篇 |
1978年 | 4篇 |
1977年 | 5篇 |
1974年 | 3篇 |
1973年 | 5篇 |
1971年 | 4篇 |
1967年 | 7篇 |
1966年 | 9篇 |
排序方式: 共有2529条查询结果,搜索用时 31 毫秒
21.
Kenichiro Ishida Yusuke Katayama Tetsuhisa Kitamura Tomoya Hirose Shunichiro Nakao Jotaro Tachino Yutaka Umemura Takeyuki Kiguchi Tasuku Matsuyama Kosuke Kiyohara Takeshi Shimazu Mitsuo Ohnishi 《Journal of pediatric surgery》2021,56(5):1013-1019
PurposeThe aim of this study was to assess the association between the implementation of abdominal angiography and outcome among pediatric patients with blunt splenic or hepatic injury.MethodsThis was a retrospective observational study, with a study period of 14 years, from January 2004 to December 2017. Blunt-trauma patients with splenic or hepatic injury who were less than 19 years old were included in this study. We used propensity-score-(PS) matching analysis to assess the relationship between abdominal angiography and in-hospital mortality.ResultsIn total, 639 patients were eligible for analysis, with 257 patients included in the abdominal-angiography group and 382 patients in the no-abdominal-angiography group. After PS matching, 224 patients from each group were selected. In the PS matched patients, in-hospital mortality was lower in the abdominal-angiography group than in the no-abdominal-angiography group (4.9% vs. 11.2%, odds ratio 0.416, 95% confidence interval 0.177–0.903).ConclusionIn this population, the implementation of abdominal angiography was significantly associated with lower in-hospital mortality among pediatric patients with blunt splenic or hepatic injury compared with nonimplementation of abdominal angiography.Type of studyPrognosis study.Level of evidenceIII 相似文献
22.
BackgroundMethotrexate (MTX) or mycophenolate mofetil with tacrolimus (TAC) is used for graft-vs-host disease (GVHD) prophylaxis in unrelated cord blood transplantation (CBT). However, there is no consensus regimen for GVHD prophylaxis in CBT. We aimed to assess the efficacy and feasibility of minimum-dose, short-term MTX (MS-MTX) for GVHD prophylaxis in CBT.MethodsWe retrospectively evaluated 35 consecutive adult patients who underwent CBT and received MS-MTX (6 mg/m2 day 1; 3 mg/m2 days 3 and 6, intravenously) with TAC for GVHD prophylaxis in our hospital between 2015 and 2019. Transplantation outcomes with respect to time to hematopoietic recovery, engraftment, incidence and severity of GVHD, adverse events, relapse, nonrelapse mortality (NRM), and overall survival were evaluated.ResultsThe median time to neutrophil, platelet, and reticulocyte recovery was 22, 38, and 32 days, respectively. Cumulative neutrophil engraftment was 91.4%. After a median 3.2-year follow-up, the 2-year overall survival was 64.3%. The 2-year cumulative incidence of relapse and NRM was 20.4% and 14.9%, respectively. The 100-day cumulative incidence of grade II-IV acute GVHD and 2-year cumulative incidence of chronic GVHD were 28.6% and 36.6%, respectively. No grade IV acute GVHD was observed. Sixteen patients experienced oral mucositis and/or pharyngeal pain (46%; grades 1-2, n = 15; grade 3 pharyngeal pain, n = 1). No patients suffered from human herpesvirus 6 encephalitis/myelitis.ConclusionsMS-MTX with TAC is feasible and safe and yields lower rates of severe oropharyngeal mucositis and human herpesvirus 6 encephalitis/myelitis without increasing GVHD, graft failure, relapse, or NRM. 相似文献
23.
Yanagimoto S Sone T Nagai K Otsuka N Mimura H Tomomitsu T Muranaka A Itaya M Kitayama A Fukunaga M 《Kaku igaku. The Japanese journal of nuclear medicine》1999,36(2):103-112
We have developed a simple method to correct the washout of tracer from the brain based on the two-compartment model in brain early SPECT using N-isopropyl-p-[123I]iodoamphetamine (123I-IMP). This correction was applied to a new quantitative method of regional cerebral blood flow (rCBF) in combination with the microsphere method by continuous arterial sampling previously reported. Data acquisition of 123I-IMP early SPECT was started from 35 min after 123I-IMP i.v. injection, and the time activity curve of whole brain on anterior head planar images was monitored immediately after 123I-IMP i.v. injection for the correction of washout of tracer from the brain. The usefulness of this method was evaluated in 12 patients with various brain diseases by comparison with the results obtained from the super-early SPECT at 7-10 min after 123I-IMP i.v. injection. The washout rates in cases of early SPECT corrected by this method ranged from 16.91% to 39.34% with a mean +/- SD of 27.72 +/- 5.44%. The contrast of hypo- to hyperperfusion regions on early SPECT was improved by the correction of the washout, and its intracerebral distribution was similar to the simultaneously obtained super-early SPECT images. These results indicated that the present correction method for the washout was useful for more correct quantification of rCBF. 相似文献
24.
Tomoya Matsunobu Kazuhiro Tanaka Yoshihiro Matsumoto Fumihiko Nakatani Riku Sakimura Masuo Hanada Xu Li Yoshinao Oda Ichiro Naruse Hideki Hoshino Masazumi Tsuneyoshi Hiromasa Miura Yukihide Iwamoto 《Clinical cancer research》2004,10(3):1003-1012
PURPOSE: Ewing's family tumors (EFTs) display the characteristic fusion gene EWS-Fli1. We have reported EWS-Fli1 may promote the cell cycle progression accompanied by the suppression of the expression of cyclin-dependent kinase inhibitor p27(kip1) in EFT cells. Here, we describe the prognostic and therapeutic relevance of p27 in EFTs. EXPERIMENTAL DESIGN: We examined tumor samples taken from 21 patients with primary EFTs for the expression of p27 protein immunohistochemically and evaluated its correlation with clinical outcome. We also investigated the usefulness of p27 as a therapeutic strategy in vitro and in vivo using p27 expression adenovirus. Finally, we examined the process of EWS-Fli1-mediated reduction of p27 expression. RESULTS: Immunohistochemical analysis showed that a low expression level of p27 protein was related to poor event-free survival in an univariate analysis and that the expression level of p27 correlated more significantly with patient survival than several clinical factors in a multivariate survival analysis. Overexpression of p27 with the adenoviral vector remarkably inhibited the cell growth in all EFT cells tested and further induced apoptosis in the wild-type p53 EFT cells. In vivo studies demonstrated a reduction in tumor growth of EFT xenograft in nude mice treated with the intratumoral injection of p27-expressing adenovirus. EWS-Fli1 did not significantly affect the p27 promoter activity and p27 mRNA levels. However, the challenge of the proteasome inhibitor caused accumulation of p27 protein in EFT cells. These data strongly suggest EWS-Fli1 might attenuate p27 protein level via activation of the proteasome-mediated degradation pathway. CONCLUSIONS: Our findings provide the first evidence of the prognostic relevance of p27 expression in EFTs. We propose p27 as a novel and powerful therapeutic factor for the molecular target therapy of EFTs. 相似文献
25.
Kamata S Kitayama Y Usui N Kuroda S Nose K Sawai T Okada A 《Journal of pediatric surgery》2000,35(4):655-657
A case of patent ductus venosus (PDV) presenting intrapulmonary shunting is described. Although retrograde venography of ductus venosus showed few intrahepatic branches, banding of PDV resulted in increased intrahepatic portal branches and disappearance of symptoms 10 months after the operation. Banding of the ductus venosus may be effective in PDV even with hypoplastic intrahepatic portal system. 相似文献
26.
27.
Tomoya Hirota Hiroto Tsuboi Mana Iizuka-Koga Hiroyuki Takahashi Hiromitsu Asashima Masahiro Yokosawa 《Modern rheumatology / the Japan Rheumatism Association》2017,27(3):457-465
Objective: To investigate the effects of transgenic rice seeds expressing the altered peptide ligand (APL) of human glucose-6-phosphate-isomerase (hGPI325–339) in mice model of GPI-induced arthritis (GIA).Methods: We generated transgenic rice expressing T-cell epitope of hGPI325–339 and APL12 contained in the seed endosperm. The transgenic rice seeds were orally administered prophylactically before the induction of GIA. The severity of arthritis and titers of serum anti-GPI antibodies were evaluated. We examined for IL-17 production in splenocytes and inguinal lymph node (iLN) cells, and analyzed the expression levels of functional molecules in splenocytes.Results: Prophylactic treatment of GIA mice with APL12 transgenic (APL12-TG) rice seeds significantly reduced the severity of arthritis and titers of serum anti-GPI antibodies compared with non-transgenic (Non-TG) rice-treated mice. APL12-TG and hGPI325–339 transgenic (hGPI325–339-TG) rice seeds improved the histopathological arthritis scores and decreased IL-17 production compared with non-TG rice-treated mice. APL12-TG rice-treated GIA mice showed upregulation of Foxp3 and GITR protein in CD4?+?CD25?+?Foxp3+?cells in the spleen compared with non-TG rice- and hGPI325–339-TG rice-treated mice.Conclusion: APL12-TG rice seeds improved the severity of GIA through a decrease in production of IL-17 and anti-GPI antibodies via upregulation of Foxp3 and GITR expression on Treg cells in spleen. 相似文献
28.
Kaori Maeyama Kazumi Tomioka Hiroaki Nagase Mieko Yoshioka Yasuko Takagi Takeshi Kato Masami Mizobuchi Shinji Kitayama Satoshi Takada Masashi Nagai Nana Sakakibara Masahiro Nishiyama Mariko Taniguchi-Ikeda Ichiro Morioka Kazumoto Iijima Noriyuki Nishimura 《Journal of autism and developmental disorders》2018,48(5):1483-1491
Association of congenital cytomegalovirus (CMV) infection with autism spectral disorder (ASD) has been suggested since 1980s. Despite the observed association, its role as a risk factor for ASD remains to be defined. In the present review, we systematically evaluated the available evidence associating congenital CMV infection with ASD using PubMed, Web of Science, Cochrane Library, and Embase databases. Any studies on children with CMV infection and ASD were evaluated for eligibility and three observational studies were included in meta-analysis. Although a high prevalence of congenital CMV infection in ASD cases (OR 11.31, 95% CI 3.07–41.66) was indicated, too few events (0–2 events) in all included studies imposed serious limitations. There is urgent need for further studies to clarify this issue. 相似文献
29.