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71.
72.
Possible contribution of follicular interleukin-1beta to nitric oxide generation in human pre-ovulatory follicles 总被引:2,自引:0,他引:2
Tao M; Kodama H; Kagabu S; Fukuda J; Murata M; Shimizu Y; Hirano H; Tanaka T 《Human reproduction (Oxford, England)》1997,12(10):2220-2225
The aim of this study was to investigate the relationships between
follicular nitric oxide (NO) metabolite concentrations and several related
variables, with special reference to follicular interleukin- 1beta
(IL-1beta). The follicular fluid from the leading and secondary follicles
was collected individually from 20 women undergoing in-vitro fertilization
(IVF) treatment, and the concentrations of nitrite (NO2-) and nitrate
(NO3-) were determined fluorometrically using 2,3- diaminonaphthalene. Both
follicular nitrite (r = 0.42, P < 0.01) and nitrate (r = 0.49, P <
0.001) were found to be significantly correlated with follicular IL-1beta
concentrations. There were also significant positive correlations between
follicular nitrate and the number of oocytes retrieved (P < 0.01) and
serum oestradiol concentration on the day of human chorionic gonadotrophin
(HCG) administration (P < 0.05). When follicular cells were incubated in
vitro with 10 ng/ml of IL-1beta for 24 h, nitrate generation was
significantly (P < 0.01) elevated compared with the control. In
conclusion, our study demonstrates that follicular IL-1beta and the number
of developing follicles are significant variables that affect follicular NO
concentrations, and points to the possible contribution of IL-1beta to NO
generation in human preovulatory follicles.
相似文献
73.
Granulocytosis associated with malignant neoplasms: a clinicopathologic study and demonstration of colony-stimulating activity in tumor extracts 总被引:1,自引:0,他引:1
We studied seven cases of malignant neoplasms, taken from various sites in the body, that were associated with marked granulocytosis. The seven cases were characterized clinically by marked granulocytosis with mature neutrophils, nonspecific inflammatory signs, and a rapid and progressive fatal course of the disease. The elevation of granulocyte count generally paralleled the increase in tumor size. Postmortem examination revealed no evidence of extensive bone marrow metastases or significant suppuration in any case. The bone marrows showed varying degrees of granulocytic hyperplasia with or without a shift to the left in the maturation series. Erythroid cell hyperplasia was observed in some cases, and in one instance there was an increase in immature eosinophils. The spleen showed various degrees of infiltration by neutrophils, from minimal to extremely marked; some spleens had foci of extramedullary hemopoiesis. Colony-stimulating activity was demonstrated in tumor extracts from three of these cases and from the serum in another case. Thus, it is suggested that marked granulocytosis in these patients was caused, at least in part, by colony-stimulating factor produced by the neoplastic cells. 相似文献
74.
Numerical aberrations of chromosome 9 in bladder cancer. A possible prognostic marker for early tumor recurrence 总被引:3,自引:0,他引:3
Tsukamoto M Matsuyama H Oba K Yoshihiro S Takahashi M Naito K 《Cancer Genetics and Cytogenetics》2002,132(1):41-45
Bilharzial bladder cancer is one of the most common types of malignancy in both men and women in several developing countries including Egypt. It has several unique clinical, epidemiological, and histological characteristics, suggesting that it is an entity distinct from bladder cancer seen in Western countries. Genetic alterations in bilharzial-related bladder cancer have been studied infrequently, especially in the advanced stages of disease, that is, T3 and T4 classifications. The objective of this study was to extend establishing the baseline cytogenetic profile of this type of malignancy to early T1 and T2 classifications. For this purpose, fluorescence in situ hybridization was applied to interphase nuclei of frozen-stored samples with biotinylated repetitive DNA probes specific for all chromosomes to detect numerical chromosome changes in 35 patients presenting with relatively early-stage pT1 and pT2 disease. Eleven cases had squamous cell carcinoma (SCC) and 24 had transitional cell carcinoma. Six of 24 transitional cell carcinomas had diploid chromosome counts with all the probes. Numerical chromosome aberrations were detected in 18 cases (75%). In 12 cases, a loss of chromosome 9 was observed. In three cases, an additional loss of chromosome 17 was detected. One case demonstrated a loss of chromosome 10, whereas another two cases showed a gain of chromosome 7, next to a loss of chromosome 9. Loss of chromosome Y was observed in nine of the 27 male cases studied (33.3%), in which only one case showed an abnormality whereas four cases were detected next to loss of chromosome 9, and one case showed gain of chromosome 7. Five cases showed loss of chromosome 19 whereas gain of chromosome 4 was detected in two cases. Two of 11 samples of SCC had normal diploid chromosome counts with all the probes used. In four of 11 cases (36.4%) underrepresentation of chromosome 9, compared with the other chromosomes, was detected. An additional loss of chromosome 17 and gain of chromosome 7, next to loss of chromosome 9, was detected in three cases. One case showed loss of chromosome 17 as the only numerical aberration. Loss of the Y chromosome was detected in three cases of which one case had gain of chromosome 7 and one case had loss of chromosome 19. No correlation was found between any of the clinicopathologic parameters examined in this study and the presence or absence of any numerical chromosomal aberrations except for the significant association between schistosomal history and loss of Y chromosome (P=0.007). 相似文献
75.
Kazuo Umetsu Isao Yuasa Takao Yamashita Susumu Saito Tomio Yamaguchi Srinama B. Ellepola Takafumi Ishida Tsuneo Suzuki 《Journal of human genetics》1989,34(3):195-202
The genetic polymorphism of orosomucoid (ORM) and alpha-2-HS-glycoprotein (AHSG) were studied in Thai, Sri Lankan and Paraguayan populations using isoelectric focusing. Gene frequencies in these populations were compared with those in other populations. Four new ORM variants were detected:ORM1
*
15 in Thai,ORM1
*
16 in Paraguayan,ORM2
*
21 andORM2
*
22 in Sri Lankan. 相似文献
76.
Kazuo Takayama Kanichi Yagawa Akiko Takahashi Hironori Nishio Morio Sudo Nobuyuki Sasaki Toru Yoshida Masanobu Satoh Hisaaki Abiko Akio Nunokawa Mitsuo Okazaki 《Pathology international》1969,19(4):547-562
A typical case of the D uchenne type of progressive muscular dystrophy with autopsy findings was presented. Changes in the myocardial and smooth muscle of many organs were found, and the skeletal muscles also revealed florid changes.
Histopathological examination of the skeletal muscle was made in detail through light and electron microscopic observation. 相似文献
Histopathological examination of the skeletal muscle was made in detail through light and electron microscopic observation. 相似文献
77.
Inhibition of p38MAP kinase suppresses fibrotic reaction of retinal pigment epithelial cells 总被引:3,自引:0,他引:3
78.
Kazuo Soga Takeshi Shiono Hyun Joon Kim 《Macromolecular chemistry and physics.》1993,194(12):3499-3504
A modified SiO2 was prepared by reacting SiO2 with Cl2Si(CH3)2 in toluene, on which methylaluminoxane (MAO) was supported to obtain a catalyst precursor. The mixture of the precursor and Cp2ZrCl2 (Cp: cyclopentadienyl) gave polyethylene in a high yield even by using common trialkylaluminiums as cocatalyst. Surprisingly, the MAO-free catalyst system composed of the modified SiO2 and Cp2ZrCl2 was also found to be activated by common trialkylaluminiums. 相似文献
79.
Evaluation of a Membrane Filter Assay System, Ortho HCV Ab Quik Pack, for Detection of Anti-Hepatitis C Virus Antibody 下载免费PDF全文
Takanari Kodama Satoshi Ichiyama Kumiko Sato Toshi Nada Nobuo Nakashima 《Journal of clinical microbiology》1998,36(5):1439-1440
A simple membrane immunoassay assay system, Quik Pack, for the detection of hepatitis C virus antibody was compared with two enzyme-linked immunosorbent assays (ELISAs) in a study of 600 serum samples. Quik Pack exhibited excellent sensitivity and specificity: 96.0 and 99.7%, respectively, versus the ELISA-2 and 99.7 and 99.4%, respectively, versus the ELISA-3. 相似文献
80.
Medaka fish (Oryzias latipes) were exposed to various doses of X-rays or fast neutrons, and the frequency of micronucleated cells (MNCs) was measured in gills sampled at 12- or 24-hr intervals from 12 to 96 hr after exposure. The resulting time course of MNC frequency was biphasic, with a clear peak 24 hr after exposure, irrespective of the kind of radiation applied and the dose used. The half-life of MNCs induced in the gill tissues by the two exposures fluctuated around 28 hr, with no significant dose-dependent trend for either X-ray- or neutron-exposed fish. As assayed 24 hr after exposure, the MNC frequency increased linearly over the control level with increasing doses of both X-rays and fast neutrons. The relative biological effectiveness (RBE) of fast neutrons to X-rays for MNC induction was estimated to be 4.3 +/- 0.6. This value is close to the RBE value of 5.1 +/- 0.3 reported for fast neutron induction of somatic crossing-over mutations in Drosophila melanogaster that arise from recombination repair of DNA double-strand breaks. These results and other data support our conclusion that the medaka gill cell micronucleus assay is a reliable short-term test for detecting potential inducers of DNA double-strand breaks. 相似文献