全文获取类型
收费全文 | 1672篇 |
免费 | 91篇 |
国内免费 | 18篇 |
专业分类
耳鼻咽喉 | 10篇 |
儿科学 | 40篇 |
妇产科学 | 26篇 |
基础医学 | 261篇 |
口腔科学 | 38篇 |
临床医学 | 116篇 |
内科学 | 270篇 |
皮肤病学 | 39篇 |
神经病学 | 204篇 |
特种医学 | 42篇 |
外科学 | 99篇 |
综合类 | 3篇 |
预防医学 | 263篇 |
眼科学 | 13篇 |
药学 | 173篇 |
中国医学 | 23篇 |
肿瘤学 | 161篇 |
出版年
2023年 | 16篇 |
2022年 | 21篇 |
2021年 | 37篇 |
2020年 | 24篇 |
2019年 | 41篇 |
2018年 | 39篇 |
2017年 | 39篇 |
2016年 | 36篇 |
2015年 | 32篇 |
2014年 | 63篇 |
2013年 | 71篇 |
2012年 | 117篇 |
2011年 | 147篇 |
2010年 | 64篇 |
2009年 | 70篇 |
2008年 | 125篇 |
2007年 | 138篇 |
2006年 | 135篇 |
2005年 | 132篇 |
2004年 | 114篇 |
2003年 | 110篇 |
2002年 | 91篇 |
2001年 | 7篇 |
2000年 | 6篇 |
1999年 | 7篇 |
1998年 | 13篇 |
1997年 | 6篇 |
1996年 | 6篇 |
1995年 | 12篇 |
1994年 | 5篇 |
1993年 | 3篇 |
1992年 | 6篇 |
1989年 | 4篇 |
1988年 | 7篇 |
1987年 | 2篇 |
1986年 | 2篇 |
1985年 | 2篇 |
1984年 | 2篇 |
1983年 | 4篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1978年 | 1篇 |
1977年 | 4篇 |
1976年 | 3篇 |
1975年 | 2篇 |
1974年 | 2篇 |
1973年 | 1篇 |
1972年 | 2篇 |
1971年 | 1篇 |
1968年 | 1篇 |
排序方式: 共有1781条查询结果,搜索用时 46 毫秒
61.
Úrsula Cataln Anna Pedret Silvia Yuste Laura Rubi Carme Piol Berner Andre Sandoval-Ramírez Judit Companys Elisabet Foguet Pol Herrero Núria Canela Maria-Jose Motilva Rosa Sol 《Nutrients》2022,14(5)
The impact of a red-fleshed apple (RFA) rich in anthocyanins (ACNs), a white-fleshed apple (WFA) without ACNs, and an extract infusion from Aronia fruit (AI) equivalent in dose of cyanidin-3-O-galactoside (main ACN) as RFA was determined by the proteome profile of aorta and heart as key cardiovascular tissues. Hypercholesterolaemic Wistar rats were separated into six groups (n = 6/group; three males and three females) and the proteomic profiles were analyzed using nanoliquid chromatography coupled to mass spectrometry. No adverse events were reported and all products were well tolerated. RFA downregulated C1QB and CFP in aorta and CRP in heart. WFA downregulated C1QB and CFP in aorta and C9 and C3 in aorta and heart, among other proteins. AI downregulated PRKACA, IQGAP1, and HSP90AB1 related to cellular signaling. Thus, both apples showed an anti-inflammatory effect through the complement system, while RFA reduced CRP. Regardless of the ACN content, an apple matrix effect was observed that involved different bioactive components, and inflammatory proteins were reduced. 相似文献
62.
Coronary artery spatial distribution of chronic total occlusions: Insights from a large US registry
下载免费PDF全文
![点击此处可从《Catheterization and cardiovascular interventions》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Santiago Garcia MD M. Chadi Alraies MD Aris Karatasakis MD Demetris Yannopoulos MD Dimitri Karmpaliotis MD Khaldoon Alaswad MD Farouc A. Jaffer MD PhD Robert W. Yeh MD MBA Mitul P. Patel MD John Bahadorani MD Judit Karacsonyi MD Pratik Kalsaria MS Barbara Danek MD Subhash Banerjee MD Emmanouil S. Brilakis MD PhD 《Catheterization and cardiovascular interventions》2017,90(1):23-30
63.
64.
Engineering lysine reactivity as a conformational sensor in the Dictyostelium myosin II motor domain
Kovács M Tóth J Málnási-Csizmadia A Bagshaw CR Nyitray L 《Journal of muscle research and cell motility》2004,25(1):95-102
Lys84 of skeletal muscle myosin located at the interface between the motor and neck domains has long been utilized as a useful chemical probe sensing motor domain conformational changes and tilting of the lever arm. Here we report the first site-directed mutagenesis study on this side chain and its immediate chemical environment. We made Dictyostelium myosin II motor domain constructs in which Lys84 was replaced by either a methionine or a glutamic acid residue and another mutant containing an Arg704Glu substitution. By following trinitrophenylation of the mutant constructs, we first unambiguously identify Lys84 as the reactive lysine in Dictyostelium myosin. Analysis of the reaction profiles also reveals that the Lys84-Arg704 interaction at the interface of two subdomains of the myosin head has a significant effect on Lys84 reactivity, but it is not the only determinant of this property. Our findings imply that the nucleotide sensitivity of the trinitrophenylation reaction is a general feature of conventional myosins that reflects similar changes in the conformational dynamics of the different orthologs during the ATPase cycle. 相似文献
65.
66.
Havasi V Szolnoki Z Talián G Bene J Komlósi K Maász A Somogyvári F Kondacs A Szabó M Fodor L Bodor A Melegh B 《Journal of molecular neuroscience : MN》2006,29(2):177-183
The possible pathogenic role of triglycerides (TG) in the development of ischemic stroke is still under extensive investigation.
Recently, apolipoprotein (apo)A5 gene promoter region T-1131C polymorphism has been shown to associate with elevated serum
TG levels. In the current work, a total of 302 subjects were classified as being large vessel-associated, small vessel-associated,
or belonging to a mixed group of ischemic stroke-affected patients. The level of TG was increased in all groups (p<0.01). The apoA5-1131C allele frequency was approximately twofold in all groups of stroke patients compared with the controls
(5 vs 10–12%; p<0.05); and the apoA5-1131C allele itself was also found to associate with increased TG levels in all groups. In a multivariate
logistic regression analysis model adjusted for differences in age, gender, serum cholesterol, hypertension, pressence of
diabetes mellitus, smoking and drinking habits, and ischemic heart disease, a significantly increased risk of developing stroke
disease was found in patients carrying the apoA5-1131C allele (p<0.05; odds ratio OR=2.1 [1.3–4.7]); this association was also proven for all subtypes of the stroke. The results presented
here suggest that the apoA5-1131C allele is an independent risk factor for the development of stroke. Being that apoA5 gene
is under the control of the peroxisome proliferator-activated receptor α, theoretically, the current observations also can
have long-term therapeutic consequences. 相似文献
67.
Zsuzsa Menczel Mark D. Griffiths István Vingender Andrea Eisinger Judit Farkas Anna Magi Barbara Mervó Gyöngyi Kökönyei Zsolt Demetrovics 《International journal of mental health and addiction》2017,15(3):580-587
Research has demonstrated that exercising has health promoting effects. However, if habitual sporting activities become uncontrollable, detrimental health consequences can occur among a minority of individuals. Furthermore, such obligatory exercise can cause serious decline in school/work productivity, as well as financial problems, relationship problems, and poor psychological and physical wellbeing. The aim of the present study was to compare characteristics related to exercise dependence (ED) between recreational exercisers and amateur competitors. A total of 1439 participants (41.4 % male; mean age = 32 years) completed a battery of measures including the Exercise Dependence Scale (EDS), SCOFF, Well-Being Questionnaire, and Rosenberg’s Self-Esteem Scale. Results showed that 6.5 % of participants identified themselves as amateur competitive exercisers. Amateur competitors exercised significantly more (6.4 h) than non-competitor recreational exercisers (4.6 h). Amateur competitors also scored significantly higher on the EDS. Significant effects were found between competing and self-esteem concerning ED. Results showed that both features had a strong effect on ED. The study highlights the connection between ED symptoms and lower self-esteem and/or lower levels of subjective wellbeing. These influential factors are worth considering when planning preventive interventions addressing ED for both amateur competitors and non-competitive recreational athletes as well as when promoting sport as a healthy activity. 相似文献
68.
Judit García-Villoria Laura Gort Irene Madrigal Carme Fons Cristina Fernández Aleix Navarro-Sastre Montserrat Milà Paz Briones Angeles García-Cazorla Jaume Campistol Antonia Ribes 《European journal of human genetics : EJHG》2010,18(12):1353-1355
17β-Hydroxysteroid dehydrogenase 10 (HSD10) is a mitochondrial enzyme involved in the degradation pathway of isoleucine and branched-chain fatty acids. The gene encoding HSD10, HSD17B10, has been reported as one of the few genes that escapes X-inactivation. We previously studied two female patients with HSD10 deficiency, one of them was severely affected and the other presented a mild phenotype. To elucidate as to why these two carriers were so differently affected, cDNA analyses were performed. The HSD17B10 cDNA of eight control cell lines, two hemizygous patients and two carriers was obtained from cultured fibroblasts, amplified by PCR and sequenced by standard methods. All HSD17B10 cDNAs were quantified by real-time PCR. In the fibroblasts of the female patient who presented with the severe phenotype, only the mutant allele was identified in the cDNA sequence, which was further confirmed by relative quantification (RQ) of HSD17B10 cDNA. This is in agreement with an unfavourable X-inactivation. The other female patient, with slight clinical affectation, showed the presence of both mutant and wild-type alleles in the cDNA sequence, which was confirmed by RQ of HSD17B10 cDNA in fibroblasts. This is in line with normal X-inactivation and the expression of both alleles in different cells (functional mosaicism). RQ results of HSD17B10 cDNA did not differ significantly between male and female controls, which indicate that the genetic doses of mRNA of HSD17B10 was the same in both sexes. In conclusion, these results suggest that the HSD17B10 gene does not escape X-inactivation as has been reported previously. 相似文献
69.
Kolev K Tenekedjiev K Ajtai K Kovalszky I Gombas J Váradi B Machovich R 《Blood》2003,101(11):4380-4386
Myosin modulates the fibrinolytic process as a cofactor of the tissue plasminogen activator and as a substrate of plasmin. We report now that myosin is present in arterial thrombi and it forms reversible noncovalent complexes with fibrinogen and fibrin with equilibrium dissociation constants in the micromolar range (1.70 and 0.94 microM, respectively). Competition studies using a peptide inhibitor of fibrin polymerization (glycl-prolyl-arginyl-proline [GPRP]) indicate that myosin interacts with domains common in fibrinogen and fibrin and this interaction is independent of the GPRP-binding polymerization site in the fibrinogen molecule. An association rate constant of 1.81 x 10(2) M(-1) x s(-1) and a dissociation rate constant of 3.07 x 10(-4) s(-1) are determined for the fibrinogen-myosin interaction. Surface plasmon resonance studies indicate that fibrin serves as a matrix core for myosin aggregation. The fibrin clots equilibrated with myosin are stabilized against dissolution initiated by plasminogen and tissue-type plasminogen activator (tPA) or urokinase (at fibrin monomer-myosin molar ratio as high as 30) and by plasmin under static and flow conditions (at fibrin monomer-myosin molar ratio lower than 15). Myosin exerts similar effects on the tPA-induced dissolution of blood plasma clots. Covalent modification involving factor XIIIa does not contribute to this stabilizing effect; myosin is not covalently attached to the clot by the time of complete cross-linking of fibrin. Thus, our in vitro data suggest that myosin detected in arterial thrombi binds to the polymerized fibrin, in the bound form its tPA-cofactor properties are masked, and the myosin fibrin clot is relatively resistant to plasmin. 相似文献
70.