卵清白蛋白特异性T细胞免疫诱导BALB/c小鼠的调节性免疫应答

目的：研究T细胞免疫后正常小鼠的调节性免疫应答，方法：应用体外扩增的卵清白蛋白（OVA）特异的T细胞克隆免疫BALB／c小鼠，3H－TdR掺入法分析细胞增殖，3H－TdR标记靶细胞检测杀伤T细胞的杀伤效应，间接免疫荧光法分析血清中抗T细胞抗体水平。结果：T细胞免疫后能诱导BALB/c小鼠产生调节性T细胞的增殖反应，对靶细胞的杀伤效应以及针对于活化的T细胞的体液免疫应答，并进一步降低机体对OVA抗原的应答，结论：T细胞免疫能诱导正常机体的调节性免疫应答。     

Pre-S_2、抗Pre-S_2和HBV DNA在乙型肝炎患者中的相关性分析

目的 :探讨Pre_S2 、抗Pre_S2 二对半和HBVDNA对乙型肝炎患者血清学的诊断意义。方法 :用ELISA法和PCR法分别检测 93例乙型肝炎患者和 2 8例健康人血清中的Pre_S2 ,抗Pre_S2 ,二对半和HBVDNA水平。结果 :93例乙肝患者HBsAg、HBeAg、HBcAb阳性 32例 ,HBsAg、HBeAb、HBcAb阳性 39例 ,HBsAg、HBcAb阳性 18例 ,HBcAb阳性 4例。Pre_S2 结果阳性率 6 6 .7% (6 2 / 93) ,Pre_S2 抗体结果阳性率 1.1% (1/ 93) ,HBVDNA结果阳性率40 .9% (38/ 93)。 2 8例健康人二对半 ,五项全阴 16例 ,HBsAb阳性 12例 ,Pre_S2 阳性 1例 ,HBVDNA阳性 1例 ,Pre_S2 抗体阳性 5例占HBsAb阳性 41.7% (5 / 12 ) ,Pre_S2 和HBVDNA相比有明显差异 (P <0 .0 1) ,非HBeAg阳性组中HBVDNA阳性率约低于Pre_S2 。结论 :Pre_S2 ,抗Pre_S2 ,HBVDNA和二对半在乙型肝炎血清学诊断中显示 ,Pre_S2 优于HBVDNA和二对半。Pre_S2 抗体出现早于抗HBs和抗HBe ,抗Pre_S2 阳性检出率较低 ,可能同乙型肝炎疫苗中抗Pre_S2 含量低有关。     

变性高效液相色谱法检测CpG岛胞嘧啶甲基化

目的 建立一种新型的ＣpＧ岛胞嘧啶甲基化快速检测方法。方法 用亚硫酸氢钠处理ＤＮＡ,丙用链特异性聚合酶链反应（ＰＣＲ）对错配修复基因hＭＬＨ１启动子含ＣpＧ位点的靶序列进行扩增;利用变性高效液相色谱法（ＤＨＰＬＣ）在部分变性温度下测定靶序列的保留时间,并与亚硫酸氢钠－酶切法测定结果进行比较。结果 用ＤＨＰＬＣ对结肠癌细胞株ＲＫＯ和胃癌细胞株ＰＡＣＭ８２的hＭＬＨ１启动子进行测定,发现ＲＫＯ细胞ＰＣＲ产物的保留时间显长于ＰＡＣＭ８２细胞ＰＣＲ产物的保留时间（６．７min比６．２min）。ＲＫＯ细胞ＰＣＲ产物保留时间的延长是亚硫酸氢钠处理后的模板中胞嘧啶和鸟嘌呤含量较ＰＡＣＭ８２高所致。从此结果分析,可以判断出ＲＫＯ的hＭＬＨ１启动子已甲基化,而ＰＡＣＭ８２细胞未甲基化。此结果与酶切法结果完全一致。结论 新方法可以快速检测ＣpＧ岛胞嘧啶甲基化。     

Blue toe syndrome: treatment with percutaneous atherectomy

"Blue toe syndrome" refers to digital ischemia of the foot in the presence of palpable or Doppler audible pedal pulses. This clinical syndrome is caused by microembolization to small vessels from a proximal source. The use of percutaneous transluminal atherectomy is described in the treatment of embologenic superficial femoral artery lesions in seven patients. All seven had prompt healing of the ischemic toes, and none required surgical revascularization or amputation. One patient developed a recurrent stenosis at the atherectomy site and had a second episode of digital ischemia, which was treated by means of atherectomy with a larger device. Histologic study of atherectomy specimens suggests that emboli arise from adherent fibrinoplatelet aggregates or thrombus and less often from cholesterol-rich atheromatous plaque. Although either percutaneous transluminal angioplasty or atherectomy can be used to treat the underlying stenosis, percutaneous atherectomy offers the advantage of nonsurgical removal of embologenic material and provides material for histologic study. Percutaneous atherectomy is an effective method of treating embologenic superficial femoral stenoses in patients with ipsilateral blue toe syndrome.     

替拉万星(telavancin):一种新型脂糖肽类抗菌药

替拉万星(telavancin,TD-6424)是万古霉素的衍生物,用于治疗耐药革兰阳性菌,特别是MRSA感染。本文对替拉万星作用机制、抗菌活性、药动学、药效学、临床适应证和不良事件等作一简要介绍。一、作用机制     

Stratum corneum lipids liposomes for the topical delivery of 5-aminolevulinic acid in photodynamic therapy of skin cancer: preparation and in vitro permeation study

Background  

Photodynamic therapy (PDT) using 5-aminolevulinic acid (5-ALA) is a skin cancer therapy that still has limitations due to the low penetration of this drug into the skin. We have proposed in this work a delivery system for 5-ALA based on liposomes having lipid composition similar to the mammalian stratum corneum (SCLLs) in order to optimize its skin delivery in Photodynamic Therapy (PDT) of skin cancers.