RETRACTED ARTICLE: MiR-200 suppresses metastases of colorectal cancer through ZEB1

Poor prognosis of some colorectal cancer (CRC) cases largely results from early metastases of CRC to the distal organs. Thus, suppression of the invasion of CRC appears to be crucial therapy. Since microRNAs (miRNAs) play critical roles in the regulation of cancer metastases, identification of the involved miRNAs may provide novel therapeutic targets for CRC treatment. Here, we showed that the levels of miR-200 were significantly decreased and the levels of ZEB1 were significantly increased in the CRC specimens from patients, compared to the paired non-tumor tissue. Moreover, the levels of miR-200 and ZEB1 are inversely correlated. Bioinformatics analyses showed that miR-200 targeted the 3′-UTR of ZEB1 mRNA to inhibit its translation, which was confirmed by luciferase reporter assay. Moreover, miR-200 overexpression inhibited ZEB1-mediated cell invasiveness, while miR-200 depletion increased ZEB1-mediated cell invasiveness in CRC cells. Together, our data suggest that miR-200 suppression in CRC cells may promote ZEB1-mediated cancer metastasis. Our work thus highlights a novel molecular regulatory machinery that regulates metastases of CRC.     

Long-term effect of acetyl-L-carnitine on myocardial123I-MIBG uptake in patients with diabetes

Carnitine derivatives may have beneficial effects on cardiac and nerve function in patients with diabetes. The aim of this study was to investigate the effect of acetyl-L-carnitine (ALC) on myocardial sympathetic nervous function as measured with¹²³I-meta-iodobenzyl guanidine (MIBG) and single-photon emission tomography (SPET) in 19 patients with diabetes (placebo group,n=6; ALC group,n=13) at the beginning and at the end of a 1-year randomized, placebo-controlled, doubleblind trial. The coefficient of variation for the MIBG analysis was 4%. In patients who were given a placebo, global myocardial MIBG uptake deteriorated during the study (MIBG uptake 1-year follow-up/baseline, 0.86±0.05, mean±standard error of mean), whereas in patients treated with ALC, MIBG uptake did not change significantly (1-year follow-up/baseline, 1.07±0.08; p=0.03 between the groups). On the basis of these preliminary data, we conclude that long-term treatment with ALC may be of potential value in preventing the progressive loss of myocardial sympathetic nervous function in patients with diabetes. MIBG-SPET is a sensitive and thus valuable method in assessing the development of myocardial sympathetic nervous dysfunction.     

13例阑尾黏液性肿瘤的影像表现分析

目的 探讨阑尾黏液性肿瘤的影像表现.方法 回顾性分析本院2015年6月至2019年12月经手术病理证实的13例阑尾黏液性肿瘤患者的影像资料,11例行CT平扫及增强扫描,2例行CT平扫检查,其中1例CT平扫后行MR平扫加增强检查,分析检测结果.结果 8例阑尾低级别黏液性肿瘤CT表现呈长茄形、类圆形、葫芦形、香肠形,囊壁厚...     

单纯性浅表皮肤感染小鼠模型的建立及有效性验证

建立与临床上单纯性浅表皮肤感染较为接近的小鼠模型,便于致病机制和外用抗菌药物筛选及疗效观察研究。选用BALB/c小鼠,采用胶带反复粘贴法破坏局部皮肤屏障,通过皮肤水分流失(TEWL)值标定皮肤屏障破坏程度,然后在其创面接种金黄色葡萄球菌(7.5×10⁶ CFU/mL)及化脓性链球菌(1.5×10⁸ CFU/mL),建立局部皮肤的实验感染模型,并给予氧氟沙星凝胶以验证模型的敏感性和有效性。结果显示:胶带反复粘贴7次和10次分别为金黄色葡萄球菌和化脓性链球菌感染前皮肤屏障破坏的最佳次数;6株金黄色葡萄球菌和3株化脓性链球菌局部感染后,创面活菌培养结果分别为4.99±4.74、5.52±5.45、4.92±4.56、5.24±4.84、5.20±5.01、4.94±4.69和4.82±4.87、5.34±5.45、4.63±4.41 CFU/g,与对照组比较差异均有统计学意义(P<0.01)。给予氧氟沙星凝胶后,对感染灶细菌生长的抑制率均大于80%。结果表明,所建立的皮肤浅表感染模型具有一定的敏感性和有效性,对皮肤浅表感染发病机制研究、外用抗菌药物筛选和疗效观察具有一定的应用前景。     

Asymptomatic long-segmental type A aortic dissection diagnosed by transthoracic echocardiography: A case report and literature review

Aortic dissection (AD) is a life-threatening disease. Patients usually experience severe pain in the chest, back, or abdomen, but some patients report a variety of other symptoms without pain. Completely asymptomatic AD is sporadic and probably under-recognized. The present study aimed to (a) report an extremely rare case of asymptomatic long-segmental type A AD, wherein exaggeratedly low diastolic blood pressure and broad pulse pressure were the primary signs, and (b) summarize the clinical characteristics of asymptomatic AD through the literature review.     

Efficient synthesis of niobium pentoxide nanowires and application in ethanolysis of furfuryl alcohol

Nb₂O₅ nanowires with high specific surface area and crystallinity were prepared by using ammonium oxalate and an acetic acid solvent system. The nanomaterial was applied in ethanolysis of furfuryl alcohol (FA), and the yield of the product, 2-(ethoxymethyl)furan (FEE), achieved was up to 79.6%. Compared to mesoporous Nb₂O₅ materials and other porous materials, the residence time of FEE on the surface of the catalyst is shorter, and the yield of ethyl levulinate (EL) is lower. Furthermore, a high temperature calcination treatment can change the acid sites and acidity type distribution on the nanowire surface. By XRD, NH₃-TPD, IR, and TG-DTA determination methods, it was found that the weak and medium-strong acid sites on the surface of Nb₂O₅ nanowires were reduced after a 300 °C treatment, and the amount of strong acid was relatively higher. According to the catalytic performance test data and acidity determination, it was concluded that more weak acid and medium-strong acid sites improve the conversion of furfuryl alcohol to FEE, and the strong acid sites promote further conversion of FEE to EL.

Nb₂O₅ nanowires with high specific surface area and crystallinity were prepared by using ammonium oxalate and an acetic acid solvent system.     

血清超敏C反应蛋白、肿瘤坏死因子α对经皮冠状动脉介入术患者预后的影响

目的探讨血清超敏C反应蛋白(high-sensitivity C-reactive protein,hs-CRP)、肿瘤坏死因子α(tumor necrosis factor-alpha,TNF-α)对经皮冠状动脉介入术(percutaneous coronary intervention,PCI)患者预后的影响。方法选择我院2016年1月至2017年12月收治的197例行PCI治疗的急性冠状动脉综合征(acute coronary syndrome,ACS)患者的临床资料进行分析,将患者按PCI术后1年是否出现主要不良心血管事件(major adverse cardiovascular events,MACE)分为MACE组和非MACE组。比较两组患者PCI前及术后48 h血清hs-CRP、TNF-α浓度,采用Logistic回归分析法分析PCI后患者发生MACE的危险因素。结果PCI后1年随访结果,197例ACS患者PCI后发生MACE 39例,发生率为19.8%(39/197)。MACE组39例,非MACE组158例。MACE组和非MACE组患者PCI前血清hs-CRP分别为(9.70±4.71)、(7.50±4.61)mg/L,PCI后48 h分别为(15.37±5.01)、(12.16±4.38)mg/L,两组治疗前后及治疗后比较差异均有统计学意义(P均<0.01)。MACE组和非MACE组患者PCI前血清TNF-α分别为(33.1±8.9)、(25.7±8.0)ng/L,PCI后48 h分别为(47.6±8.1)、(32.4±7.6)ng/L,两组治疗前后及治疗后比较差异均有统计学意义(P<0.05或P<0.01)。Logistic回归分析结果表明,术前血清hs-CRP、TNF-α浓度为PCI患者发生MACE的危险因素[OR(95%CI)分别为2.069(1.715~3.358)、2.825(1.614~4.372),P值分别为0.020、0.027]。结论血清hs-CRP、TNF-α浓度与PCI患者预后有关,PCI术前血清CRP、TNF-α浓度均为发生MACE的危险因素,可作为发生MACE的独立预测因素。     

影响冠心病病人同型半胱氨酸水平的主要非遗传因素分析

目的 分析影响冠心病人血浆同型半胱氨酸水平的主要非遗传因素.方法 164例住院冠心病患者,测定血浆同型半胱氨酸水平并分析其与全血叶酸、血浆维生素B12、血清雌二醇浓度以及冠心病传统危险因素之间的关系.结果 血浆同型半胱氨酸水平与叶酸、维生素B12水平呈负相关,男性血浆同型半胱氨酸浓度高于女性[（20.74±13.42）×10-6mol/L比（15.56±8.16） ×10-6mol/L,P〈0.05],吸烟者高于不吸烟者[（22.29±15.18）×10-6mol/L比（17.21±9.54） ×10-6mol/L,P〈0.05].结论 叶酸、维生素B12、性别和吸烟是影响冠心病患者血浆同型半胱氨酸水平的主要非遗传因素.