壮族人和汉族人高血压与心肌肥厚关系探讨

目的:通过对壮族人和汉族人原发性高血压与左室心肌肥厚关系的研究,从侧面探讨两个民族的原发性高血压的等位基因是否相同,从而指导临床的治疗用药.方法:壮族组30例,汉族组40例,两组患者均为原发性高血压病人.采用彩色多谱勒超声仪进行心脏检查,测量相关径线并进行比较.结果:两组患者的心超测值及左室心肌肥厚情况无显著性差异.结论:壮族人与汉族人原发性高血压左室心肌肥厚情况无差异,说明两者的等位基因有可能相同.     

二尖瓣脱垂与急性风湿性心脏炎的相关性研究

目的探讨二尖瓣脱垂(MVP)与急性风湿性心脏炎(ARC)的相关性。方法对108例风湿热并ARC的患者行超声心动图检查以观察MVP的发生率。结果超声心动图检查发现MVP76例,发生率为70％,其中前叶脱垂57例(53％),后叶脱垂13例(12％),前后叶均脱垂6例(6％)。与对照组比较有明显的差异(P＜0．01)。ARC所致MVP中74例(97％)并发二尖辫关闭不全(MI),且MVP与MI的严重程度密切相关(P＜0．01)。结论ARC是MVP,尤其是二尖瓣前叶脱垂的重要原因之一,而MVP是ARC所致MI的重要因素。     

妊娠期糖尿病对妊娠结局的影响

目的研究GDM对母婴的影响。方法对112例GDM孕妇和112例健康孕妇的妊娠结局进行分析。采用11配对病例—对照研究,配对的条件是孕妇年龄相近(±2),同年同月同医院分娩。结果GDM孕妇手术分娩率、早产率、妊娠高血压综合征和巨大儿发生率都较对照组高。GDM的及早诊断和及时治疗可使巨大儿发生率降低。与新生儿病率有关的因素是GDM的严重程度、血糖控制情况及巨大儿。结论早期诊断GDM及控制血糖是减少妇婴并发症的关键。     

氧化低密度脂蛋白、低氧和5-HT对血管平滑肌细胞5-HT2A受体及胞内游离钙的影响

目的　为探讨粥样硬化病变动脉对 5 - HT收缩反应增加的机制 ,观察与粥样硬化有关因子氧化低密度脂蛋白 (ox L DL)、低氧、5 - HT对血管平滑肌细胞 5 - HT2 A受体及其基因表达 ,以及细胞 [Ca2 ]i的效应。方法　分别将大鼠主动脉平滑肌细胞单独以 5 0 μg/ m l ox L DL 培养 8h,2 %低氧处理 2 4和 48h,10 μm ol/ L 5 - HT处理30 min,放射配基结合分析测定 5 - HT2 A受体 ;RT- PCR和 Southern杂交检测受体基因的 m RNA;激光共聚焦扫描显微镜测单个细胞 [Ca2 ]i。结果　经 ox L DL、低氧、 5 - HT处理 ,血管平滑肌细胞 5 - HT2 A受体显著上调 (P<0 .0 1) ;ox L DL及低氧处理的受体基因的 m RNA表达增加 ;ox L DL、低氧处理后 ,5 - HT刺激的血管平滑肌细胞[Ca2 ]i升高幅度显著加大 (P<0 .0 5及 P<0 .0 1)。结论　 ox L DL、低氧、5 - HT引起血管平滑肌细胞 5 - HT2 A受体上调及细胞 [Ca2 ]i增加 ,可能是粥样硬化动脉对 5 - HT收缩反应增强的部分原因。     

增生性瘢痕和瘢痕疙瘩组织中Ⅰ、Ⅲ型前胶原及TGF-β_1 mRNA的表达

目的：探讨增生性瘢痕（Ｈ）和瘢痕疙瘩（Ｋ）组织中Ⅰ、Ⅲ型前胶原及ＴＧＦ－β１基因表达改变的相互关系及临床意义。方法：总ＲＮＡ抽提试剂盒抽提总ＲＮＡ,斑点杂交检测Ⅰ、Ⅲ型前胶原及ＴＧＦ－β１ｍＲＮＡ稳态水平的改变。结果：Ｈ和Ｋ组织中ＴＧＦ－β１ｍＲＮＡ（１．１９７±０．２３７,１．２０４±０．２４３）表达均高于正常瘢痕和正常皮肤（０．３２７±０．０８１,０．３３１±０．０７８）,Ｐ＜０．０１;Ｋ选择性地Ⅰ型前胶原ｍＲＮＡ表达增强,而Ｈ组织中Ⅰ、Ⅲ前胶原ｍＲＮＡ表达均增强,导致ＫⅠ、Ⅲ前胶原ｍＲＮＡ比值明显高于Ｈ（８．１６４±０．３００,１．６６６±０．２０１,Ｐ＜０．０１）。结论：Ｋ和Ｈ组织中胶原蛋白基因表达类型及强度不同,提示在Ｋ和Ｈ发生发展中具有不同的分子机理：ＴＧＦ－β１在增生性瘢痕疙瘩的发病机理中具有不同的分子机理;ＴＧＦ－β１在增生性瘢痕和瘢痕疙瘩的发病机理中具有一定作用。     

1,25-二羟维生素D_3抑制报导载体pGL_2荧光素酶表达的研究

通过磷酸钙沉淀法将载体DNA转染Caco－2细胞。Caeo－2细胞经不同浓度1,25一二羟维生素D3处理后,测定表达的荧光素酶活性。结果;当pSG5－VDR表达载体共转染时1,25一二羟维生素D3显著地抑制Caco－2细胞荧光素酶的表达;而未使用pSG5－VDR表达载体共转染时,则无抑制作用。说明1,25一二羟维生素D3能抑制报导载体pGL2荧光素酶的表达。类似于人类PTH基因中的潜在抑制性VDRE存在于报导载体pGL2。     

Morphological and functional demonstration of rat dura mater mast cell-neuron interactions in vitro and in vivo

The effects of changes in temperature on primary and secondary endings of isolated cat muscle spindles were investigated under ramp-and-hold stretches and different degrees of pre-stretch. Temperature-induced alterations of the discharge frequency were compared over a temperature range of 25–35°C. Both primary and secondary endings responded to warming with increasing discharge frequencies when the spindle was pre-stretched by 5–10% of its in situ length. The following differences between the temperature effects on primary and secondary endings were observed: (1) The temperature coefficients (Q₁₀) obtained from the discharge frequencies during the dynamic and static phase of a stretch were similar for endings of the same type, but they were larger in primary endings (range of Q₁₀: 2.3–3.3; mean: 2.9) than in secondary endings (range of Q₁₀: 1.6–2.2; mean: 2.0); (2) With primary endings, but not with secondary endings, the temperature sensitivity (imp s⁻¹ °C⁻¹) was larger during the dynamic phase than during the static phase of a stretch; (3) In primary endings, the fast and slow adaptive components occurring in the discharge frequency during the static phase of a stretch clearly increased with warming while in secondary endings, the slow decay was less affected, and the fast decay showed no change; (4) In relaxed spindles, the excitatory effect of warming was overlaid by a strong inhibitory effect as soon as the temperature exceeded about 30°C, resulting in an abrupt cessation of the background activity in most secondary endings, but not usually in primary endings. In general, warming induced an enhanced stretch sensitivity in both types of ending, and additionally an inhibitory effect that is obvious only in secondary endings of relaxed spindles. The different effects of temperature on the discharge frequency of primary and secondary afferents are assumed to be caused by different properties of their sensory membranes.     

An analysis of an air-ambulance program for children

Air-ambulance services are extremely expensive to operate and maintain. The value of their existence has been questioned in this era of rationalization and downsizing. We examined the cost, safety, flight crew composition, types of trauma, and effectiveness of our air-ambulance program at the Children's Hospital of Eastern Ontario (CHEO) over a 3-year period, 1994-1997. During this time, 392 children were aeroevacuated to CHEO, 113 surgical (29%), 136 medical (35%), and 143 neonates (36%). Of the surgical cases, 43% were transferred for orthopaedic trauma, the commonest being fractured femur, 23% for general surgical cases, most common being thoracoabdominal trauma, and 22% for neurosurgical reasons, most commonly basilar skull fractures. The average response time (time from which the call was received to the time when the helicopter reached the patient site) for all of the cases was 46 min. The average travel time (time from departure of patient site to arrival at CHEO) for these same cases was 25.59 min. The air-ambulance program for children in the Ottawa-Carleton Eastern Ontario areas, was found to be safe, effective, and allowed earlier specialized medical care to be provided.