抗氧化性维生素与男性生殖

生殖系统内过多的活性氧 (ROS)产生可导致精子膜脂质过氧化损伤 ,而抗氧化性维生素对于清除ROS 避免精子脂质过氧化损伤具有一定保护作用。本文就抗氧化性维生素在男性生殖中的作用及其在男性不育治疗等方面进行了综述     

高职贫困生应激生活事件、社会支持与孤独感的关系：SEM建构

目的构建高职贫困生社会支持、应激生活事件与孤独感的关系模型,检验社会支持在结构方程中的中介效应。方法对361名高职贫困生分别采用社会支持量表、青少年社会生活事件问卷和孤独感问卷进行测评,并分析其内在关系,进而构建潜变量间的结构方程模型,并对模型进行最优地修正。结果结构方程分析表明,高职贫困生应激生活事件和社会支持对孤独感有直接作用力,社会支持在应激生活事件对孤独感影响中起到中介作用。结论应激生活事件、社会支持对高职贫困生孤独感的作用得到结构方程的有力支持。     

Expression of Clock genes in the pineal glands of newborn rats with hypoxic-ischemic encephalopathy

Clock genes are involved in circadian rhythm regulation, and surviving newborns with hypoxic-ischemic encephalopathy may present with sleep-wake cycle reversal. This study aimed to determine the expression of the clock genes Clock and Bmal1, in the pineal gland of rats with hypoxic-ischemic brain damage. Results showed that levels of Clock mRNA were not significantly changed within 48 hours after cerebral hypoxia and ischemia. Expression levels of CLOCK and BMAL1 protein were significantly higher after 48 hours. The levels of Bmal1 mRNA reached a peak at 36 hours, but were significantly reduced at 48 hours. Experimental findings indicate that Clock and Bmal1 genes were indeed expressed in the pineal glands of neonatal rats. At the initial stage (within 36 hours) of hypoxic-ischemic brain damage, only slight changes in the expression levels of these two genes were detected, followed by significant changes at 36-48 hours. These changes may be associated with circadian rhythm disorder induced by hypoxic-ischemic brain damage.     

Correlation of aquaporin-4 expression to blood-brain barrier permeability in rats with focal cerebral ischemia

BACKGROUND: Ischemic cerebrovascular disease causes injury to the blood-brain barrier. The occurrence of brain edema is associated with aquaporin expression following cerebral ischemia/reperfusion. OBJECTIVE: To analyze the correlation of aquaporin-4 expression to brain edema and blood-brain barrier permeability in brain tissues of rat models of ischemia/reperfusion. DESIGN, TIME AND SETTING: The randomized control experiment was performed at the Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, China from December 2006 to October 2007. MATERIALS: A total of 112 adult, male, Sprague-Dawley rats, weighing 220-250 g, were used to establish rat models of middle cerebral artery occlusion and reperfusion by the suture method. Rabbit anti-aquaporin-4 (Santa Cruz, USA) and Evans blue (Sigma, USA) were used to analyze the tissue. METHODS: The rats were randomized into sham-operated (n = 16) and ischemia/reperfusion (n = 96) groups. There were 6 time points in the ischemia/reperfusion group, comprising 4, 6, 12, 24, 48, and 72 hours after reperfusion, with 16 rats for each time point. Rat models in the sham-operated group at 4 hours after surgery and rat models in the ischemia/reperfusion group at different time points were equally and randomly assigned into 4 different subgroups. MAIN OUTCOME MEASURES: Brain water content on the ischemic side and the control side was measured using the dry-wet weight method. Blood-brain barrier function was determined by Evans Blue. Aquaporin-4 expression surrounding the ischemic focus, as well as the correlation of aquaporin-4 expression with brain water content and Evans blue staining, were measured using immunohistochemistry and Western blot analysis. RESULTS: Brain water content on the ischemic side significantly increased at 12 hours after reperfusion, reached a peak at 48 hours, and was still high at 72 hours. Brain water content was greater on the ischemic hemispheres, compared with the control hemispheres at 6, 12, 24, 48, and 72 hours after reperfusion, as well as both hemispheres in the sham-operated group (P<0.05). Evans blue content significantly increased on the ischemic side at 4 hours after ischemi',dreperfusion, and reached a peak at 48 hours. Evans blue content was greater on the ischemic hemispheres, compared with the control hemispheres at various time points, as well as both hemispheres in the sham-operated group (P<0.05). Aquaporin-4-positive cells were detected in the cortex and hippocampus, surrounding the ischemic penumbra focus, at 4-6 hours after ischemia/reperfusion. The number of positive cells significantly increased at 12 hours and reached a peak at 48-72 hours. Aquaporin-4 was, however, weakly expressed in the control hemispheres and the sham-operated group. The absorbance ratio of aquaporin-4 to β-actin was greater at 12, 24, 48, and 72 hours following cerebral ischemia/reperfusion, compared with the sham-operated group (P<0.05). Aquaporin-4 expression positively correlated to brain water content and Evans blue staining following cerebral ischemia/reperfusion (r1 = 0.68, r2= 0.81, P<0.05). CONCLUSION: Aquaporin-4 is highly expressed in brain tissues, participates in the occurrence of ischemic brain edema, and is positively correlated to blood-brain barrier permeability following cerebral ischemia/reperfusion.     

大学生营养评价方法的研究

目的　探讨客观的营养状况评价方法 ,了解大学生营养状况。　方法　采用随机抽样法抽取 1～ 4年级 185名 18～ 2 5岁大学生 ,测量身高、体质量、臂围、三头肌皮脂厚度 ;采用体质量指数、BMI指数、三头肌皮脂厚度、上臂肌围等方法对大学生的营养状况进行分析。　结果　被调查的大学生 60 %左右体质量正常 ,但体质量不足和超重现象也同时存在。不同的评价方法其结果差异明显。　结论　应选用不同的评价指标 ,综合评价大学生的营养状况 ,对于改善营养状况干预措施的制定和实施 ,更具意义     

大学生自评与测验人格特质的差异及其影响因素分析

目的 探讨大学生自评与测验人格特质的差异及其影响因素。方法 以“16PF”为工具，让大学生在充分了解16种根源特质的内涵以后，进行人格自我评价，并完成“16PF”人格测验。并以“SCL—90”症状自评量表和“FES—CV”家庭环境量表的因素作为自变量，探讨影响其差异因素。结果 研究表明16种根源特质中的乐群性、稳定性、有恒性、怀疑性、世故性、忧虑性、独立性、自律性、紧张性因素存在自评人格特质与测验人格特质的显著性差异。结论 发现心理健康因素、家长教养方式、家庭氛围和家长教育观念是主要的影响因素。     

柏枣宁心颗粒治疗顽固性失眠症疗效观察

笔者自2004年1月至2008年1月,根据辨证论治以自拟柏枣宁心颗粒治疗顽固性失眠症,取得了较好疗效。现将结果报道如下。     

论医科院校准确定位与科学发展——以蚌埠医学院为例

对大学准确的目标定位是办好大学的基础，定位决定了大学的规格和模式，也决定了大学发展的方向和目标。大学在选择目标定位时既要根据经济和社会发展的需求，又要根据自身的历史和现实条件；既要量力而行，实事求是，又应寻找特色，大胆突破。文章以蚌埠医学院为例，讨论了医学院校准确定位与科学发展的关系。     

枳实对大鼠小肠电活动影响的下丘脑作用机制研究

目的观察枳实对在体大鼠小肠电活动的影响,探讨其作用与下丘脑内脑肠肽胆囊收缩素(CCK)、生长抑素(SS)的相关性。方法采用消化道腔外电极记录法观察枳实组与生理盐水组移行性综合肌电(MMC)周期时程、活动期/周期比例、活动期每分钟快波数的变化；采用免疫组织化学法结合显微图像定量分析扫描系统检测下丘脑背内侧核(DMH)、腹内侧核(VMH)、下丘脑外侧区(LHA)CCK的相对含量,以及下丘脑VMH、LHA、室旁核(PVN)SS的相对含量。结果枳实组与生理盐水组比较MMC周期缩短、活动期/周期比例增大、活动期每分钟快波数增加,差异有显著性；与生理盐水组及空白对照组比较枳实组DMH、VMH、LHA的CCK阳性神经元表达降低；VMH、LHA、PVN的SS阳性神经元表达增高,与生理盐水组及空白对照组比较差异有显著性。结论枳实对大鼠小肠电活动有兴奋作用,这种兴奋作用可能与下丘脑内的CCK、SS有关。     

活血化瘀对冠心病胰岛素抵抗的研究

目的 :探讨活血化瘀法对冠心病胰岛素抵抗的临床疗效。方法 :观察 46例冠心病患者接受活血化瘀治疗前后空腹血糖、胰岛素、胰岛素敏感性指数的变化 ,评定其临床疗效。结果 :治疗前后比较 ,空腹血糖无差异 (P >0 .0 5 ) ,胰岛素水平治疗后明显下降 (P <0 .0 5 ) ,胰岛素敏感性指数显著增高 (P <0 .0 5 )。结论 :活血化瘀法具有改善冠心病患者胰岛素抗抵作用。