糖尿病性心血管病变与血清微量元素铁、铬、锰水平的相关性研究

本文研究糖尿病并发心血管病变时血清脂类、脂类过氧化产物与微量元素水平的相关性,结果发现:血清微量元素铁(Fe)与总胆固醇(T—ch)水平呈负相关,与水溶性荧光物质(WSFS)呈正相关;血清微量元素锰(Mn)与极低密度脂蛋白—胆固醇(VLDL—ch)呈负相关,与丙二醛(MDA)呈正相关;微量元素铬(Cr)与高密度脂蛋白—胆固醇(HDL—ch)呈正相关。提示:微量元素Fe、Cr、Mn为糖尿病性心血管病变的易患元素。     

术前静脉造影对静脉管壁及其吻合效应的实验研究

以动物实验模拟行静脉造影后,肉眼观察,并以透射电镜和扫描电镜动态观察经灌注固定的局部静脉管壁和内皮细胞的改变,发现造影剂回流的静脉管壁在较长的时间内处于扩张状态,同时穿刺注射造影剂的部位出现较严重的淤血和血栓形成。内皮细胞连接有较广泛裂开、剥离及内皮下组织暴露。在受损严重的区域还可见有微血栓形成。造影1天后内皮细胞受损最为严重,至第3天基本恢复。血管吻合实验表明,术前3天静脉造影不影响直径为2.5mm静脉的吻合通畅率。     

The Influence of Intramammary Lipopolysaccharide Infusion on Serum Ca,P, Vitamin D,Cytokines and Cortisol Concentrations in Lactating Sows

Ten multiparous lactating sows were used to investigate whether intramammary infusion of lipopolysaccharides (LPS; Escherichia coli 0111:B4; 2.0 μg/kg of body weight) would affect the circulating concentrations of Ca, P, 25‐hydroxyvitamin D (25‐OHD), tumour necrosis factor‐α (TNF‐α), interleukin‐6 (IL‐6) and cortisol. The sows were randomly allotted to either control group (control) or LPS‐treated group with five individuals per group and were infused with either physiological saline solution or LPS solution. The rectal temperature and udder quarter appearance were recorded at 0 (just before infusion), 1, 3, 7, 12 or 24 h after infusion. Blood samples were taken at 0, 1, 3, 7, 12 or 24 h after infusion. Before infusion, the rectal temperatures of all sows were below 39.2°C. At 3 and 7 h after infusion, the sows in the LPS group had a rectal temperature over 39.4°C. At 24 h after infusion, the rectal temperatures returned to pre‐infusion levels. Serum Ca and P concentrations in the LPS group decreased (P < 0.05) after LPS infusion compared with the control group at 1 h after infusion. No significant differences (P > 0.05) in the concentrations of 25‐OHD were observed between groups control and LPS at any sampling time. Increased (P < 0.01) concentrations of serum TNF‐α, IL‐6 and cortisol were observed in the LPS group compared with the control group at 3 and 7 h after infusion respectively. In conclusion, the elevation of serum concentrations of TNF‐α, IL‐6 and cortisol and the alterations of circulating concentrations of Ca and P following LPS infusion indicate that the immune system has been activated and immune activation may affect macromineral homeostatic regulation, which might have important implications for metabolic health of lactating sows. Lowered serum Ca and P following immune activation also shows a causative mechanism whereby immune activation increases the risk of secondary disorders such as mastitis‐metritis‐agalactia syndrome. However, immune activation did not affect circulating concentrations of vitamin D metabolites.     

重组2型腺相关病毒-转化生长因子β3对实验性肝纤维化大鼠肝脏组织病理学和Ⅰ型胶原表达的影响

Objective To investgate the effects of TGF β 3 on rat hepatic fibrosis. Methods The TGF β 3 cDNA was cloned into rAAV2 vector. Rats were randomly divided into four groups: normal control group, model group, negative control group and TGF β 3 group. Hepatic fibrosis was induced by hypodermic injection of 40% CCI4. Recombinant AAV2-TGF β 3 viral particles were injected via the vena caudalis one week before CCh treatment. Rats were executed 8 weeks after CCI4 treatment, global histological change was observed after HE staining, the distribution of the collagen fibers was observed after masson staining, his-tochemistry was done to observe the expression of collagen Ⅰ; The positive area rate of the collagen fibers and the average optical rate of collagen Ⅰ were quantified. Results HE staining indicated that collagen fibers were reduced in the TGF β 3 group. Masson staining shown that the collagen fibers were distributed around the blood vessel, in the portal area and disse space. Compared to the model group (13.2%±2.2%) and negative control group (12.3%±1.5%), the collagen fibers in liver tissues of TGF β3 group (7.7% ± 1.5%) were significantly decreased (q = 9.456, P < 0.01; q = 8.217, P < 0.01). Histochemistry indicated that the collagen fibers of liver tissues of TGF 15 3 group (0.185±0.033) were significantly higher than those in the model group (0.252±0.042) and the negative control group (0.230±0.029), (q = 6.228, P < 0.01; q = 4.346, P < 0.01). Conclusion TGF β 3 alleviates the damage to hepatic cell and the level offibrosis in CCI4 treated rats and inhibits the expression of collagen Ⅰ.     

扩散管配制甲醛标准气体的研究

介绍了扩散法配制标准气体的方法。以三聚甲醛为原料，通过加热催化分解三聚甲醛蒸气，来配制甲醛标准气体。讨论了配制甲醛标准气体的准确性、稳定性、重复使用性，以及温度、扩散管几何尺寸、载气气流、配气源物质的量等因素对三聚甲醛扩散率的影响。本方法制备的甲醛标准气体，扩散率（２～５０μｇ／ｍｉｎ）测定总不确定度小于５％。     

大鼠移植心脏组织中血小板衍生生长因子A mRNA表达及雷帕霉素的干预效应

目的:血小板衍生生长因子在平滑肌细胞的表型转化过程中起重要作用。观察大鼠移植心脏组织中血小板衍生生长因子AmRNA表达的变化及雷帕霉素的干预效应。方法:实验于2005-10/2006-01在中南大学湘雅二医院胸心外科实验室完成。将60只SD大鼠、24只Wistar大鼠按随机数字表法分为3组:①同系移植组:供、受体各12只,均为SD大鼠。②异系移植组:供体为Wistar大鼠(n=24),受体为SD大鼠(n=24),受体大鼠随机分为雷帕霉素组(n=12)和环孢霉素组(n=12),术后分别给予雷帕霉素1.25mg/(kg·d)灌胃及环孢霉素A10mg/(kg·d)皮下注射,给药60d,给药结束后留取移植心脏待检。③另12只SD大鼠直接取心脏组织作为正常对照组。指标检测:①对移植心脏组织行VanGieson染色后采用Miassystem4.1医学图像分析管理系统分析血管狭窄程度。②应用反转录-聚合酶链反应检测血小板衍生生长因子AmRNA在移植心脏组织中的表达情况。结果:36只受体SD大鼠及12只正常SD大鼠全部进入结果分析,无脱失。①同系移植组、环孢霉素组及雷帕霉素组大鼠的冠状动脉狭窄指数均显著高于正常对照组[(13.12±0.72)%,(62.45±8.12)%,(28.91±3.24)%,(0.09±0.02)%(P<0.01)],环孢霉素组及雷帕霉素组高于同系移植组(P<0.05),环孢霉素组高于雷帕霉素组(P<0.01)。②正常对照组、同系移植组、环孢霉素组及雷帕霉素组大鼠的血小板衍生生长因子AmRNA相对含量分别为0.19±0.06,0.21±0.08,1.12±0.22及0.47±0.11,环孢霉素组、雷帕霉素组显著高于同系移植组(P<0.01),环孢霉素组高于雷帕霉素组(P<0.05)。结论:血小板衍生生长因子AmRNA的高表达与移植心脏的血管硬化有关;雷帕霉素具有预防大鼠心脏移植物血管病变的作用,其作用可能与抑制心脏组织中血小板衍生生长因子AmRNA的表达有关。     

门诊老年冠心病患者用药知识需求的调查

冠心病是老年人的常见病、多发病,致残或死亡率已居常见病之首[1]。目前,药物治疗仍是冠心病二级预防的主要措施。近年来研究发现,83·3%的冠心病患者无法正确使用药物,用药依从性差[2]。分析原因,87%的门诊老年慢性病患者包括冠心病患者在内,主观不遵医用药是由于在用药过程中出现诸多用药问题时得不到相关知识信息支持所导致[3]。另外,通过调查证实冠心病患者对用药知识的需求亦居前列,尤其是出院后用药知识是首要需求[2,4-6]。由此表明,对门诊老年冠心病患者进行用药知识教育十分必要。依据健康教育学方法,分析患者的教育需求是制定教育…     

下体负压作用下心率变异性的线性和非线性动力学分析

为了评估下体负压作用下心率变异性的变化，本文应用了时域，频域分析和复杂度分析了ＨＲＶ。受试者为１５名健康男性。下体负压作用的强度和时间分别为－２．７ｋＰａ，２ｍｉｎ；－４．０ｋＰａ，３ｍｉｎ；－５．３ｋＰａ，５ｍｉｎ；－６．７ｋＰａ，１０ｍｉｎ。     

移植于脊髓的断离肋间神经重发新支至原去神经肋间肌的实验观察

将15只大鼠左侧第11或12肋间神经从根部向外,自周围组织完全分离出20～35mm,并于此处剪断。再将其近端改向植入脊髓左侧半 L_(1～2) 之间的髓节内。在存活57～413天后解剖观察,发现自移植神经的干上发出1～3条新分支,分布于原去神经的肋间肌。经光、电镜观察证实是成活的再生神经纤维。于移植神经干注射 CB-HRP 后,发现这些神经纤维来源于原肋间神经髓节的前角和移植部髓节内的神经细胞。故认为外周神经缺损,不作神经对接或桥接,不经断离神经的远段,在一定条件下可以由近段发出再生纤维组成新支,分布于去神经的靶组织。     

骨水泥性能及其对人工关节柄固定强度的影响

本文研究了骨水泥三种不同临床使用方法所形成的内部气泡结构状态,测定了骨水泥的抗压强度、收缩率、疲劳强度、变形积累量等物理机械性能,以及关节固定强度,用气泡状态解释了不同临床使用方法对骨水泥性能及固定强度所带来的各种影响。