顽固性复发性气胸胸膜腔造影及硬化治疗后追踪

对32例顽固性复发性气胸患者用60%泛影葡胺进行胸膜腔造影,确定裂口后作硬化剂治疗,并作复张后胸部CT对照及手术追踪对照,取得满意效果。     

磷酸钛钾激光声带切除术不同术式的临床特点

目的 探讨支撑喉镜下磷酸钛钾（ｐｏｔａｓｉｕｍ ｔｉｔａｎｉｕｍ ｐｈｏｓｐｈａｔｅ,ＫＴＰ）激光声带切除术不同术式的选择及愈合过程中的临床特点。方法 选择７６例早期声门型喉癌行ＫＴＰ激光声带切除术,对不同术式术后喉内结构恢复过程、音质变化等进行随访观察记录。结果 Ⅰ型术式１１例,３个月后恢复喉的正常结构及功能;Ⅱ型术式５０例,３个月后有声带样粘膜皱襞（以下称新声带）形成,基本恢复喉的结构;Ⅲ型术     

青光眼类型及静态阈值视野损害

目的:探讨青光眼类型与静态阈值视野损害间的关系.方法:对确诊的147例、228只青光眼分类进行静态阈值视野检查.结果:不同类型的青光眼在静态阈值视野缺损出现的最大象限上差异无显著性.青光眼静态阈值视野缺损最大象限的分布依次为颞上、鼻上、鼻下,颞下象限分布最少.结论:不同类型的青光眼所致静态阈值视野损害的主要机制可能是相同的.     

Partial Sequence Analysis of the Genome of Human Herpesvirus 7 YY5 Isolated from Saliva Samples

Ｈｕｍａｎｈｅｒｐｅｓｖｉｒｕｓ 7(ＨＨＶ 7)ｉｓｏｎｅｏｆｔｈｅｍｏｓｔｒｅｃｅｎｔｌｙｄｉｓｃｏｖｅｒｅｄｍｅｍｂｅｒｏｆｔｈｅｈｅｒ ｐｅｓｖｉｒｕｓｆａｍｉｌｙ .Ｉｔｗａｓｆｉｒｓｔｉｓｏｌａｔｅｄｆｒｏｍａｃｔｉｖａｔ ｅｄＣＤ4 Ｔｃｅｌｌｓｆｒｏｍｐｅｒｉｐｈｅｒａｌｂｌｏｏｄｍｏｎｏｎｕ ｃｌｅａｒｃｅｌｌｓ (ＰＢＭＣｓ)ｏｆａｈｅａｌｔｈｙｉｎｄｉｖｉｄｕａｌ[1] ,ａｐａｔｉｅｎｔｗｉｔｈｃｈｒｏｎｉｃｆａｔｉｇｕｅｓｙｎｄｒｏｍｅ(ＣＦＳ) [2 ] ,ａｎｄｆｒｏｍｓａｌｉｖａｏｆｈｅａｌ…     

医学大学生对医德认知的调查研究

采用抽样研究方法,对４３８名不同专业、不同层次的大学生进行了医德认知调查,以了解大学生对医德基本知识掌握的情况,结果表明,大学生对开设医学伦理学课必要性认识均率为６２．９％,课外经常阅读医德书籍均率为２２．７５％,对中外基德发展史各朝代典型的人物及代表作了解的均率为３１．１％,文中对调查结果进行了分析。     

Transcriptome analysis of Armillaria gallica 012 m in response to auxin

Gastrodia elata is an achlorophyllous and fully mycoheterotrophic orchid which obtains carbon and other nutrients from Armillaria species in its life cycle. Many researchers suggested that plant hormones, as signing molecules, play a central role in the plant–fungi interaction. In the process of Armillaria gallica 012 m cultivation, both exogenous indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) distinctly stimulated the growth of mycelia in solid media. The differential expression genes (DEGs) of A. gallica 012 m with IAA versus blank control (BK) and IBA versus BK were investigated. The results showed that more than 80% of DEGs of the IAA group were coincident with the DEGs of the IBA group, and more than half of upregulated DEGs and most of the downregulated DEGs of the IAA group coincided with those DEGs of the IBA group. Above research implied that A. gallica 012 m could perceive IAA and IBA, and possess similar responses and signaling pathways to IAA and IBA. The overlapping differential genes of the IAA group and IBA group were analyzed by GO term, and the results showed that several DEGs identified were related to biological processes including positive regulation of the biological process and biological process. The downregulated NmrA-like and FKBP_C genes might be benefit to the growth of mycelia. Those results can explain that exiguous IAA and IBA improved the growth of A. gallica to some extent. We speculate that IAA and IBA are signaling molecules, and regulate the expression of growth-related genes of A. gallica 012 m by the same signaling pathway.     

Poly (triethylenglycol monomethacrylate) and poly(glycerol monomethacrylate) cross-linked gel as potential viscoelastics for intraoperative use

Background: The highly swelling poly(glycerol monomethacrylate) gel (polyGLYMA) and hydrophilic polymer poly(triethylenglycol monomethacrylate (polyTEGMA) were tested as potential viscoelastics for intraopertive use in anterior segment surgery. Methods: PolyGLYMA was implanted into the anterior chamber in 5 rabbits, and 40% polyTEGMA in 16 rabbits. The eyes were enucleated 1 week to 3 months after the operation. The corneal endothelium was examined with specular microscopy, and then the whole eye histopathologically. Results: In all eyes of the polyGLYMA group, the clinical findings were characterized by a marked ciliary injection and severe secondary glaucoma, and the histologic ones by a marked inflammatory infiltration and thickening of Descemet's membrane in the anterior chamber angle. Specular microscopy revealed a decrease in the endothelial cell density and polymorphism of the endothelial cells. In the polyTEGMA group, the anterior segment and the fundus were physiologic all the time, and specular microscopy and histologic findings showed no degenerative and/or inflammatory changes. Conclusions: PolyGLYMA proved unsuitable for intracameral application in rabbits. The new polymer polyTEGMA is characterized by high biologic tolerance after its implantation into the anterior chamber of rabbits. PolyTEGMA 40% might be considered as a potential viscoelastic material in humans.     

肾性骨病的骨膜下骨吸收

笔者采用手骨直接Ｘ线放大摄影和双手Ｘ线平片来研究慢性肾衰血透病人５１例。特征性的指骨骨膜下吸收放大摄影发现２５例占４９％;平片为１１例占２１．６％,结果表明,放大摄影的阳性率明显高于平片（Ｐ＜０．００５）,骨膜下吸收的二,三指中间指骨桡侧及指骨末端是肾性骨病的“靶区”,认为二者无显著差异（Ｐ＞０．５０）。骨膜下吸收还与血透时间,血磷,血钙和碱性磷酸酶水平相关。     

Microdissection and microcloning of chromosomal alterations in human breast cancer

Summary The recognition of recurring sites of chromosome changes in malignancies has greatly facilitated the identification of genes implicated in the pathogenesis of human cancers. Based especially upon recent studies [1–4], it appears increasingly likely that a subset of recurring chromosome alterations will be recognized in human breast cancer. Currently recognized chromosome changes characterizing breast carcinoma include the recognition of cytologic features of gene amplification (e.g. double minutes [dmins] and homogeneously staining regions [HSRs]) [5–8]. As these and other chromosome regions are implicated in recurring abnormalities in breast cancer, it will become increasingly important to have band-or region-specific genomic libraries and probes in order to facilitate high resolution physical mapping and ultimately to clone breast cancer related genes [9]. Toward this end an important recent development in physical mapping has been the establishment of chromosome microdissection as a rapid and reproducible approach to rapidly isolate and characterize chromosome region-specific DNA, greatly facilitating the initial steps in positional cloning of disease-related genes [10–13]. In this brief report, we will highlight the application of chromosome microdissection to the generation of region-specific probes for both fluorescent in situ hybridization (FISH) and the generation of genomic microclone libraries. Additionally, efforts using this methodology to generate a microclone library encompassing the early onset breast/ovarian cancer (BRCA1) gene will be presented.Presented by Jeffrey M. Trent at the 16th Annual San Antonio Breast Cancer Symposium, San Antonio TX, USA, November 4, 1993; Minisymposium on Molecular Genetics in Breast Cancer.