上海市闵行区流动人口中的孕产妇保健状况

通过对上海市闵行区580名外来孕产妇及580名作为对照的本地孕产妇的孕产期保健现况调查分析,表明外来孕产妇缺乏必要的孕产期保健措施,有47.6％的外来孕产妇从未做产前检查,产后保健则完全没有,并由此而造成外来产妇在急产、死产、甚至孕产妇死亡等方面令人忧虑的现状。为适应和促进当前经济发展的新形势,改善流动人口的孕产妇保健状况,应对流动人口中孕产妇保健状况深入研究,并采取相应的措施。     

癫痫持续状态大鼠的蓝斑组织化学与图像分析研究

作者用马桑内酯致大鼠癫痫持续状态,取蓝斑用荧光组织化学方法显示去甲肾上腺素(NA),并用显微摄影自动曝光时间和图像分析两种方法测定荧光强度。结果:均显示大脑皮质包埋马桑内酯微粒的大鼠癫痫持续发作6小时后,蓝斑中NA荧光增强。用单胺氧化酶组化和图像分析法观察到该处酶活性增强。观察到在癫痫持续状态时NA起了一定的调控作用。     

钩端螺旋体核糖体提取物免疫保护作用的研究

作者对核糖体现有的制备方法进行了部分改进,从黄疸出血群赖型017株钩体中成功地制备了核糖体提取物。该提取物经SPA-ELISA 证实具有免疫原性,对40只受试豚鼠的保护试验结果表明,核糖体提取物对同型毒株的攻击具有明显的保护作用;结果还证明核糖体提取物能诱导机体的体液免疫反应。     

2型糖尿病患者载脂蛋白E基因多态性的研究

目的：探讨中国人2型糖尿病患者载脂蛋白E（apoE)基因多态性及其与血脂和载脂蛋白水平的关系。方法：采用聚合酶链反应－限制性酶切片段长度多态性法，分别对74例2型糖尿病患者及191例血脂、血糖正常且无糖尿病史者的apoE基因型、空腹血脂及载脂蛋白AI、AⅡ、B100、CⅡ、CⅢ及E进行全面分析。结果：2型糖尿病患者的血清甘油三酯（TG）、总胆固醇（TC），低密度脂蛋白胆固醇（LDLC），非高密度脂蛋白胆固醇（nHDLC),载脂蛋白B100、CⅡ、CⅢ、E水平及TG／HDLC比值较对照组显著升高（P＜0．01）；血清高密度脂蛋白胆固醇（HDLC），apoE/apoCⅢ比值显著降低（P＜0．05）。2型糖尿病组与对照组apoE基因频率分布无显著性差异（P＞0．05）。携带ε2等位基因组血清TG／HDLC比值较E3／3基因型显著降低；而携带ε4等位基因组血清apoAⅡ水平较E3／3基因型及携带ε2等位基因组显著升高（P＜0．001）。结论：2型糖尿病患者apoE基因多态性与血TG／HDLC及apoAⅡ有一定关联。     

Hemoperitoneum secondary to rupture of cystic artery pseudoaneurysm

BACKGROUND:Spontaneous hemoperitoneum of hepato- biliary origin is commonly due to hemorrhage from a liver tumor.It is rarely caused by spontaneous rupture of aneurysm in visceral arteries. METHODS:We report an unusual case of hemoperitoneum caused by rupture of cystic artery pseudoaneurysm,and also outline the approach to its management through surgical and radiological methods. RESULTS:In our patient,the pseudoanurysm was initially treated with percutaneous thrombin injection.However this method of treatment failed after initial success.The pseudoanurysm was finally obliterated successfully using microcoil embolization. CONCLUSIONS:The mainstay of treatment of cystic artery pseudoaneurysm is cholecystectomy and ligation of the aneurysm.Recent publications showed success in using microcoil embolisation.In this case we also outline the use of percutaneous thrombin injection as a definitive treatment method and discuss its success or failure as a new method of treatment.     

海岛纤恙螨经卵传递恙虫病立克次体的研究

1985年8月～1986年11月,作者以我国发现的恙螨新种海岛纤恙螨子代幼虫656只,分为21组,叮咬健康小鼠,从子1代到子4代幼虫叮咬过的小鼠中,连续分离出7株恙虫病立克次体,证明海岛纤恙螨能够经卵传递恙虫病立克次体至少4代.同时在该螨的卵、幼虫、若虫、成虫组织细胞内发现有恙虫病立克次体寄生,并发现有分裂增殖形态的立克次体,证明海岛纤恙螨各变态期内均保有恙虫病立克次体,这些立克次体能在恙螨体内大量增殖,连续传代.此项研究结果,对于确认海岛纤恙螨为浙江沿海地区恙虫病的传染媒介和宿主,提供了重要的科学依据.     

The effects in the rat of two fragments of parathyroid hormone-related protein on uterine contractions in situ.

Synthetic parathyroid hormone fragment PTH(1-34) has been reported recently to inhibit uterine contractions stimulated by a variety of agonists. We have studied the effect in this system of the parathyroid hormone-related protein fragment PTHrP(1-34) which shows 60% homology with PTH over the first thirteen amino acid residues. The effects of two different PTHrP fragments on acetylcholine-stimulated uterine contractions in vitro were studied. Whereas synthetic hPTHrP(75-86 amide) (10(-9)-10(-7) M) was without effect, synthetic hPTHrP(1-34) (10(-9)-10(-7) M) was capable of inhibiting, in a dose-related fashion, uterine muscle contractions precontracted with 10(-6) M-acetylcholine. In a second series of experiments the bovine PTH(3-34) fragment itself was shown to have no stimulatory effect on acetylcholine-stimulated contractions. Also this fragment in an equimolar concentration (10(-7) M) failed to antagonize the effects of PTHrP(1-34) on acetylcholine-stimulated uterine contractions. However, a 100-fold excess molar concentration of bPTH(3-34) (10(-6) M) completely abolished the inhibitory action of hPTHrP(1-34) (10(-8) M) on acetylcholine-stimulated uterine contractions. These results clearly show that the inhibitory action of PTH(1-34) and PTHrP(1-34) on uterine contractions depends on the integrity of the amino-terminal region of the molecule.     

SD大鼠肺中降钙素基因相关肽的分布及缺氧对其含量的影响

本研究用免疫细胞化学方法(ABC法)观察了降钙素基因相关肽(CGRP)在SD大鼠肺中的分布,缺氧对肺中神经内分泌细胞内CGRP含餐的影响。结果表明:含CGRP的神经纤维分布在肺血管平滑肌层中及其周围、气管-支气管树的粘膜下层;含CGRP的神经内分泌细胞位于气管一支气管树的上皮层、粘液腺中和肺实质内。缺氧不引起火鼠肺的神经内分泌细胞数目改变,但可使这些细胞内的CGRP含量增加。