全文获取类型
收费全文 | 343篇 |
免费 | 20篇 |
国内免费 | 27篇 |
专业分类
耳鼻咽喉 | 4篇 |
儿科学 | 19篇 |
妇产科学 | 4篇 |
基础医学 | 37篇 |
口腔科学 | 19篇 |
临床医学 | 51篇 |
内科学 | 74篇 |
皮肤病学 | 8篇 |
神经病学 | 9篇 |
特种医学 | 28篇 |
外科学 | 21篇 |
综合类 | 19篇 |
一般理论 | 1篇 |
预防医学 | 20篇 |
眼科学 | 3篇 |
药学 | 57篇 |
中国医学 | 1篇 |
肿瘤学 | 15篇 |
出版年
2023年 | 1篇 |
2022年 | 4篇 |
2021年 | 3篇 |
2020年 | 2篇 |
2019年 | 3篇 |
2018年 | 5篇 |
2017年 | 7篇 |
2016年 | 9篇 |
2015年 | 7篇 |
2014年 | 9篇 |
2013年 | 16篇 |
2012年 | 9篇 |
2011年 | 10篇 |
2010年 | 12篇 |
2009年 | 14篇 |
2008年 | 11篇 |
2007年 | 16篇 |
2006年 | 9篇 |
2005年 | 20篇 |
2004年 | 10篇 |
2003年 | 8篇 |
2002年 | 12篇 |
2001年 | 16篇 |
2000年 | 11篇 |
1999年 | 10篇 |
1998年 | 21篇 |
1997年 | 18篇 |
1996年 | 17篇 |
1995年 | 13篇 |
1994年 | 14篇 |
1993年 | 3篇 |
1992年 | 5篇 |
1991年 | 5篇 |
1990年 | 4篇 |
1989年 | 7篇 |
1988年 | 6篇 |
1987年 | 5篇 |
1986年 | 12篇 |
1985年 | 5篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1980年 | 2篇 |
1978年 | 3篇 |
1977年 | 3篇 |
1976年 | 4篇 |
1975年 | 3篇 |
1970年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有390条查询结果,搜索用时 31 毫秒
91.
LC Ewan PJ Charleston SH Pettit 《Annals of the Royal College of Surgeons of England》2014,96(2):e9-e10
Perineal hernia is a rare complication following laparoscopic abdominoperineal resection (APR) for rectal cancer. We present two case reports of perineal hernia following laparoscopic APR and discuss their management. We suggest that they developed because the pelvic peritoneum was left open during laparoscopic APR and propose that closure of the pelvic peritoneum should be routine in this operation. 相似文献
92.
Recently regulators of G protein signalling (RGS) proteins have emerged as potential therapeutic targets since they provide an alternative method of modulating the activity of GPCRs, the target of so many drugs. Inhibitors of RGS proteins must block a protein-protein interaction (RGS-Gα), but also be cell and, depending on the therapeutic target, blood brain barrier permeable. A lead compound (1a) was identified as an inhibitor of RGS4 in a screening assay and this has now been optimised for activity, selectivity and solubility. The newly developed ligands (11b, 13) display substantial selectivity over the closely related RGS8 protein, lack the off-target calcium mobilisation activity of the lead 1a and have excellent aqueous solubility. They are currently being evaluated in vivo in rodent models of depression. 相似文献
93.
The degradation of fibrillar type II collagen is a major feature of
cartilage destruction in rheumatoid arthritis (RA). Since collagenase 3 is
produced by chondrocytes and preferentially degrades type II cartilage
collagen, it seemed likely that this enzyme would have a prominent role in
the destruction of rheumatoid joints. Using immunolocalization techniques,
we have examined and compared the production and distributions of
collagenase 1 and collagenase 3 in cells and tissues derived from
rheumatoid knee arthroplasties. Primary cultures of chondrocytes stimulated
with interleukin-1 beta showed that most of the cells produced collagenase
1, whereas only a minority (approximately 5-10%) produced collagenase 3; a
few chondrocytes demonstrated the co-ordinate production of both enzymes.
Primary cultures of rheumatoid synoviocytes produced collagenase 1, but not
collagenase 3. Both enzymes were demonstrated in the rheumatoid lesion.
Collagenase 1 was more commonly observed in both synovium and cartilage (22
of the 28 specimens), was especially prominent at cartilage erosion sites,
and most of the positive specimens demonstrated extracellular enzyme. By
contrast, collagenase 3 was observed less frequently (7/28 specimens) and
was produced by relatively few chondrocytes and synovial cells, this
usually being much less than that observed for chondrocytes of
osteoarthritic cartilage. These observations suggest different regulatory
mechanisms for the production of collagenases 1 and 3 in the rheumatoid
lesion, and demonstrate that the distribution and production of collagenase
1 are far more prevalent than those for collagenase 3.
相似文献
94.
Hereditary hemolytic anemia caused by diverse point mutations of pyruvate kinase gene found in Japan and Hong Kong 总被引:1,自引:1,他引:1
Kanno H; Wei DC; Chan LC; Mizoguchi H; Ando M; Nakahata T; Narisawa K; Fujii H; Miwa S 《Blood》1994,84(10):3505-3509
We identified four distinct point mutations in homozygous pyruvate kinase (PK) variants in Japanese and Chinese patients with chronic nonspherocytic hemolytic anemia. All gene abnormalities were missense mutations that caused single amino acid substitutions. 1261A (Q421K) and 1436A (R436H), which were identified in PK Sendai and PK Shinshu, had been found in unrelated Japanese and Amish PK variants, respectively. The clinical severity and extremely low residual erythrocyte PK activity of PK Shinshu as well as of the Amish PK might be caused partly by aberrant splicing, because the 1436A mutation changes a nucleotide at the last nucleotide in the exon 10. Recently, we diagnosed a 42-year-old Japanese woman with chronic nonspherocytic hemolytic anemia as having a homozygous PK deficiency. DNA sequencing of the variant PK gene showed a homozygous missense mutation at 1403GCT- ->GTT, resulting in a single amino acid substitution from 468la-->Val. The gene mutation is likely to impair the allostericity of this enzyme, speculated from the tertiary structure. A homozygous missense mutation in PK Hong Kong, a boy of a non-Han southern Chinese minority group, was identified in exon 7 of the human L-PK gene, 941ATT-->ACT, resulting in a single amino acid substitution from 314lle-->Thr. The R- PK activity is expected to be severely affected, because the mutated amino acid residue is located between the 313 Lys and the 315 Glu, which are very important for acid-base catalysis and magnesium binding, respectively. Both the R- and M2-type PK were shown by polyacrylamide gel electrophoresis of the PK Hong Kong erythrocyte lysate, and this is the first report of a homozygous individual whose erythrocytes contain the immature (M2)-type isozyme. 相似文献
95.
Sergio Rueda Peter Smith Tsegaye Bekele Kelly O'Brien Winston Husbands Alan Li 《AIDS care》2015,27(7):907-915
The purpose of this study is to determine the relationship between the psychosocial work environment and labor market experiences (including unemployment) on mental health among adults living with HIV. We used data provided by 538 participants at clinical and community sites across Ontario, Canada. Generalized estimating equation models showed that employment was associated with lower depressive symptoms. For employed participants, adverse psychosocial work conditions, specifically job insecurity, psychological demands, and decision authority were associated with depressive symptoms. For the entire sample, the number of adverse psychosocial work conditions was associated with higher depressive symptoms while participants working in poor quality jobs reported similar levels of depressive symptoms than those who were unemployed or not in the labor force. This study showed that poor quality employment (as assessed by having a high number of adverse psychosocial work exposures) was associated with a similar level of depressive symptoms as unemployment, suggesting that “bad jobs” may not offer the same mental health benefits as “good jobs.” Policies to improve employment outcomes should take the quality of employment into account to maximize mental health benefits as better employment may lead to better mental health. 相似文献
96.
Independent prognostic significance of a nuclear proliferation antigen in diffuse large cell lymphomas as determined by the monoclonal antibody Ki-67 总被引:12,自引:0,他引:12
Grogan TM; Lippman SM; Spier CM; Slymen DJ; Rybski JA; Rangel CS; Richter LC; Miller TP 《Blood》1988,71(4):1157-1160
To assess the prognostic significance of the growth fraction in diffuse large cell lymphoma (DLCL), we studied 105 DLCL patients with the monoclonal antibody Ki-67 applied to frozen tissue sections. Ki-67 detects a nuclear antigen associated with cell proliferation not found in resting cells. Ki-67 findings and other clinical prognostic factors were correlated with outcome using univariate and multivariate analyses in the proportional hazards model. High proliferative activity, defined as nuclear Ki-67 expression in greater than 60% of malignant cells (Ki- 67 greater than 60), was found to be a strong predictor of poor survival among these patients (P = .003, log-rank). The 19 patients with Ki-67 greater than 60% had a median survival of 8 months compared with a median survival of 39 months for the 86 patients with Ki-67 less than or equal to 60%. Examination of pretreatment clinical variables indicated the patient groups were similar with regard to age, sex, stage, B symptoms, tumor bulk, and lactate dehydrogenase (LDH). Both patient groups received comparable curative intent therapy and showed comparable complete response rate precluding treatment differences as modifying outcome. Multivariate analysis indicated Ki-67 is an independent predictor of survival (multivariate P = .006). Further statistical analysis using only B-cell DLCL patients treated with CHOP (63 patients) indicated that Ki-67 greater than 60 retained strong prediction of poor outcome (P = .002, log-rank) among this homogeneous group. We conclude that high proliferative activity (Ki-67 greater than 60) is an independent factor allowing laboratory prediction of probable poor outcome of DLCL. 相似文献
97.
Enhanced expression of transforming growth factor beta during megakaryoblastic differentiation of K562 leukemia cells 总被引:5,自引:0,他引:5
Platelet alpha granules contain several growth factors such as the transforming growth factor beta (TGF-beta) that are released during blood clotting and are thought to participate in the repair of tissue injury; however, the site of synthesis of platelet TGF-beta has not been demonstrated. We studied TGF-beta expression during megakaryoblastic differentiation of the chronic myeloid leukemia cell line K562 in vitro. These cells have mainly erythroid characteristics but acquire several megakaryoblastic properties when treated with the phorbol diester 12-0-tetradecanoyl-13-phorbolacetate (TPA). During four subsequent days of megakaryoblastic differentiation the amount of the 2.5-kilobase (kb) TGF-beta mRNA increased about eightfold, and a novel 2.3-kb mRNA species was induced in the K562 cells. This occurred concomitantly with distinct induction patterns of platelet-derived growth factor A (PDGF-A) and c-sis (PDGF-B chain) RNAs and several platelet antigens. The expression of erythroid markers such as glycophorin A decreased. Culture media of TPA-differentiated K562 cells also contained TGF-beta polypeptides as shown by a sensitive radioreceptor assay and by immunoprecipitation after metabolic labeling of the cells. These polypeptides were not seen in culture media from dimethyl sulfoxide- or sodium butyrate-treated cells. Unlike in several other cells, exogenously added TGF-beta 1 or 2 affected neither TGF- beta nor PDGF RNA expression in K562 cells. 相似文献
98.
99.
100.
T V Khroyan J Wu W E Polgar G Cami-Kobeci N Fotaki S M Husbands L Toll 《British journal of pharmacology》2015,172(2):668-680