不同浓度的臭氧对实验兔骨骼肌病理学的影响

目的 观察两种浓度的臭氧(03)对实验兔骨骼肌组织的病理变化的影响,以评估臭氧对肌纤维的氧化程度.方法 10只实验兔随机分为A、B两组.随机选择一侧大腿肌肉注射臭氧-氧气混合气体,A组为浓度50μg/ml,B组为浓度30μg/ml两组分别注射5 ml气体.注射臭氧后0.5、2 h和2周后分别对臭氧注射点、旁开1 cm和2 cm取活检组织行病理学光镜检查,对侧大腿相对应部位取活检作空白对照.结果 与空白对照组比较,病理光镜下显示A、B两组注射臭氧后0.5 h注射点、旁开1 cm见肌细胞肿胀,间隙变窄或不清,肌细胞边界不清,横纹紊乱,部分肌细胞坏死溶解,空泡变性;2 h后注射点、旁开1 cm处在肌细胞坏死溶解的基础上出现了肌细胞间隙和血管内炎症细胞浸润;2周后注射点、旁开1 cm切片显示肌细胞明显萎缩,大量纤维组织增生,部分伴有玻璃样变.上述3个时点的注射点旁开2 cm病理切片均未见异常.结论 两种浓度的臭氧肌肉注射后会产生程度相当的肌细胞损害,范围半径约2 cm,可能对炎性肌细胞或肌纤维化组织起治疗作用,2周后纤维组织损伤修复.     

Study of the mechanism of essential garlic oil inhibiting lnterleukin-1 α-induced monocyte adhesion to endothelial cells

Objective  

To observe the effects of essential garlic oil (EGO) on vascular cell adhesive molecule-1 (VCAM-1) expression of endothelial cells and monocyte-endothelial cell adhesion rate induced by interleukin-l_α(IL-l_α).     

Complete urethral injury in the penile fracture: a case report and literature review

Penile fracture is described as a traumatic rupture of the tunica albuginea caused by blunt injury to the erect penis. It usually occurs as a single rupture of the tunica albuginea in one of two corpora cavernosa; a rupture with urethral injury is an extremely rare condition. Although its diagnosis is usually clinical, ultrasound plays an important role in confirming diagnosis and identifying the site of the injury. Here, we presented a case of penile fracture with complete urethral injury. A 43-year-old male was admitted to the hospital because of trauma to the genital and dysuria following sexual intercourse. After admission, the patient was diagnosed with double penile fracture and complete urethral injury after the physical and B-ultrasound examinations. Emergency surgery to remove the hematoma and repair the urethra was performed. The patient recovered smoothly and was discharged on the third day after operation. After two months’ follow-up, the patient urinated smoothly and achieved an adequate erection without other complications. In this case, consistent with previous studies, emergency surgery for penile fracture is necessary and can preserve the urethral function and sexual function. In addition, there are two lesions in tunica albuginea in this case, so careful search for the penile shaft during the surgery is important to avoid the missed injuries. This report provides evidence of an uncommon and underreported clinical case.     

造血干/祖细胞的体外扩增培养研究

利用旋转壁式生物反应器(Rotating wall vessel,RWV)体外培养脐带血干细胞,使其大量扩增,以满足临床应用对造血干/祖细胞的数量与质量要求。从脐带血分离得到的单个核细胞(Mononuclear cells,MNC)在T-flask中培养24h,之后接种到RWV反应器中,培养200h。每24h细胞计数,测量培养基的pH和渗透压变化;在144h和197h测CD34 细胞含量并做CFU-GM半固体培养。有核细胞(Nucleated cells,NC)与CD34 细胞在第197h,分别扩增了435.5±87.6倍和32.7±15.6倍,CFU-GM(Colony-forming unit-granulocyte/macrophage)细胞扩增了21.7±4.9倍。整个培养过程中,RWV反应器中的pH和渗透压都保持在造血细胞最佳的扩增条件内,pH基本保持在7.2~7.4之间,渗透压基本保持在290~310mmol/kg之间。由于旋转壁式生物反应器(RWV)结构上的特殊性,可以保证细胞在悬浮流动的状态下生长,很好地模拟了脐带中的造血微环境,使脐带血造血干细胞在该反应器中短期内得到大量扩增。     

Exact BPF and FBP algorithms for nonstandard saddle curves

A hot topic in cone-beam CT research is exact cone-beam reconstruction from a general scanning trajectory. Particularly, a nonstandard saddle curve attracts attention, as this construct allows the continuous periodic scanning of a volume-of-interest (VOI). Here we evaluate two algorithms for reconstruction from data collected along a nonstandard saddle curve, which are in the filtered backprojection (FBP) and backprojection filtration (BPF) formats, respectively. Both the algorithms are implemented in a chord-based coordinate system. Then, a rebinning procedure is utilized to transform the reconstructed results into the natural coordinate system. The simulation results demonstrate that the FBP algorithm produces better image quality than the BPF algorithm, while both the algorithms exhibit similar noise characteristics.     

电针肾俞、膈俞、百会穴对拟血管性痴呆学习记忆障碍小鼠水迷宫实验的影响

目的　观察电针肾俞、膈俞、百会穴对拟血管性痴呆小鼠学习记忆障碍的影响。方法　复制脑缺血再灌注拟血管性痴呆小鼠模型 ,并电针肾俞、膈俞、百会穴 ,分别于术后 7天、15天、30天 ,与药物喜德镇对照 ,采用水迷宫法 ,记录游全程时间、错误次数。结果　造模导致了小鼠学习与记忆能力下降 ,表现为游全程时间延长 ,错误次数增加 ,且随着观察时间的延长 ,这种改变逐渐加重。电针和喜德镇均可使模型小鼠的游全程时间缩短 ,错误次数减少 ,但电针组于 7天和 30天时 ,成绩显著优于药物组。结论　电针肾俞、膈俞、百会穴对模型小鼠学习与记忆能力下降有改善和提高作用     

A Novel Deep Learning Model Based on Multi-Scale and Multi-View for Detection of Pulmonary Nodules

Journal of Digital Imaging - Lung cancer manifests as pulmonary nodules in the early stage. Thus, the early and accurate detection of these nodules is crucial for improving the survival rate of...     

Expression of intercellular adhesion molecule 1 (ICAM-1) is reduced in permanent focal cerebral ischemic mouse brain using an adenoviral vector to induce overexpression of interleukin-1 receptor antagonist

Our previous studies have demonstrated that overexpression of recombinant human interleukin-1 receptor antagonist protein (IL-1ra) via gene transfer can reduce ischemic brain injury. However, the mechanism of action of IL-1ra in ischemia is unclear. Since interleukin-1 can up-regulate intercellular adhesion molecules in endothelium, the present study was designed to determine whether overexpression of the IL-1ra can reduce the expression of intercellular adhesion molecule-1 (ICAM-1) after ischemic injury. Normal saline or adenovirus vector (1x109 particles) encoding the human IL-1ra gene (Ad.RSVIL-1ra) or the Escherichia coli LacZ gene (Ad.RSVlacZ) was injected into the right lateral cerebral ventricle of adult CD-1 mice. After five days, permanent middle cerebral artery occlusion (MCAO) was achieved for 24 h using an intraluminal suture. Cerebral blood flow was monitored by transcranial laser Doppler flowmetry to verify the occlusion. ICAM-1 protein was quantified using Western blot analysis and localized using immunohistochemistry. After MCAO, surface blood flow in the ischemic hemisphere was decreased to 9-11% of the baseline. There were fewer ICAM-1 positive vessels in the ischemic cortex of the Ad.RSVIL-1ra transfected mice than in the Ad.RSVlacZ transfected and saline treated mice (138+/-19 vs. 249+/-25, 284+/-22, p<0.05). Western blot analysis shows that ICAM-1 protein decreased 50-60% in the Ad. RSVIL-1ra group compared to the other two groups. There were no significant differences in the numbers of positive vessels in the ischemic basal ganglia and contralateral hemisphere among the three groups. Our studies suggest that IL-1ra overexpression can down-regulate the expression of ICAM-1 in the ipsilateral cortex in ischemic mice. Interleukin-1 may play an important role in the activation of inflammatory reaction during focal cerebral ischemia by promoting leukocyte adhesion on the endothelium cells.     

The experience in the treatment of patients with extensive full-thickness burns

The aim of the present study is to analyze the changes of cure rate and the main causes of death in the last 40 years, and to summarize our experience in the treatment of extensive full-thickness burn patients. The clinical characteristics, cure rate and main causes of death of 73 cases with total burned area more than 90% TBSA and full-thickness burn area more than 70% TBSA were analyzed retrospectively. Among them, 21 cases (28.8%) were cured and 52 cases (71.2%) died. The cure rate increased significantly in the recent years, and the main causes of death changed from shock and sepsis in the time period 1959–1978 to sepsis and MODS in the past two decades. Due to the improvement of early comprehensive management of burn shock, aggressive surgical approach to full-thickness burn wound and potent systemic supporting measures, the survival rate increased significantly.     

五种酚类抗氧化剂活性差异的理论解释

AIM: To verify the effectiveness of structure-activity relationship (SAR) and theoretical calculation methods for antioxidants. METHODS: Preliminary elucidation on the differences of activities of 5 antioxidants was performed by SAR. Then semiempirical quantum chemistry method AM1 was employed to calculate the delta HOF value, the difference between the heat of formation of antioxidant and its free radical, which was used as a theoretical parameter to elucidate the differences of activities of the antioxidants thoroughly. RESULTS: delta HOF values of antioxidants were obtained as follows: ferulic acid, 150.58 kJ.mol-1; anion of ferulic acid, 122.64 kJ.mol-1; modified ferulic acid, 137.70 kJ.mol-1; anion of modified ferulic acid, 118.99 kJ.mol-1; salvianic acid, 134.17 kJ.mol-1; rutin, 137.83 kJ.mol-1, L-EGCG, 124.39 kJ.mol-1; paeonol, 176.79 kJ.mol-1. The differences of the antioxidant activities were elucidated, and how to further enhance the antioxidant activity was investigated as well. CONCLUSION: The SAR and calculation methods are rather effective to elucidate the differences of antioxidant activities, and present some new clues for structural modification of antioxidants to increase their activities.