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本文从还原剂SnCl3用量,反应体系pH值,McAb使用浓度等方面研究了^55mTc直接法标记单克隆抗体的最佳条件,并探讨了VitC对标记反应的影响。β-ME法的单抗,三氯乙酸沉淀法测定标记率,上行纸层析法测定标记产物胶体含量。结果表明,^90mTc直接标记中还原剂SnCl2浓度以0.01-0.04mg/ml为宜,反应最适pH值在5.6-6.8,McAb最低使用浓度应为500μh/ml以上,适量V 相似文献
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双歧杆菌脂磷壁酸延缓脾脏衰老的研究 总被引:9,自引:0,他引:9
目的 研究双歧杆菌表面分子脂磷壁酸 (LTA)在D 半乳糖诱导的衰老小鼠体内 ,对脾脏指数和脾细胞DNA损伤的影响。方法 在建立D 半乳糖诱导的衰老小鼠模型的同时 ,注射双歧杆菌LTA ;然后测定模型对照组、正常对照组、试验组小鼠的脾脏指数 ,并以单细胞凝胶电泳试验检测脾脏淋巴细胞DNA氧化损伤的情况。结果 与正常对照组相比 ,模型对照小鼠的脾脏指数显著升高 (P <0 .0 5 ) ,脾淋巴细胞DNA受到了较严重的损伤 (P <0 .0 5 ) ;用双歧杆菌LTA处理后 ,试验小鼠的脾脏指数明显下降 (P <0 .0 5 ) ,脾淋巴细胞DNA的损伤程度显著降低 (P <0 .0 5 )。结论 双歧杆菌LTA能显著抑制衰老小鼠脾脏淋巴细胞DNA的氧化损伤 ,这可能与双歧杆菌抗衰老有关。 相似文献
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Huang W Sher YP Delgado-West D Wu JT Peck K Fung YC 《Annals of biomedical engineering》2001,29(7):535-551
The remodeling of the pulmonary arterial tissue in response to a step change of the oxygen concentration in the gas in which a rat lives was recorded as function of time and function of O2 concentration. Three steps of changing from 20.9% to 17.2%, 13.6%, and 10% O2 were imposed. Earlier work in our laboratory has shown that pulmonary arterial tissue remodeling is significant in the first 24 h after a step change of oxygen tension. Hence we made measurements in this period. Furthermore, data were obtained for tissue remodeling of circumferential and axial lengths of the pulmonary arteries. We recorded the activities of gene expressions in the lung tissues by microarray, determined the dose response curves of gene expression in the homogenized whole lungs with respect to four levels of O2 concentration, and obtained the time courses of gene expression in the lung parenchyma in 30 days after a step decrease of O2 concentration from 20.9% to 10%. We would like to suggest that the correlation of gene expression with physiological function parameters, i.e., time, O2 tension, blood pressure, opening angle, wall thicknesses, etc., is the way to narrow down the search for specific genes for specific physiological functions. © 2001 Biomedical Engineering Society.
PAC01: 8719Uv 相似文献
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Chun Hsing Liao Yu Tsung Huang Fang Yeh Chu Tsui Hsien Lin Po Ren Hsueh 《Journal of microbiology, immunology, and infection》2008,41(4):355-357
Time to positivity is an available parameter in automated blood culture systems. We report a patient with persistent methicillin-resistant Staphylococcus aureus bacteremia who received various regimens for treatment of methicillin-resistant S. aureus, and demonstrate that monitoring of the time to positive blood culture might be helpful in the early recognition of treatment failure. 相似文献
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Weimin Sun Ben Anderson Joy Redman Aubrey Milunsky Arlene Buller Matthew J McGinniss Franklin Quan Arturo Anguiano Stephen Huang Feras Hantash Charles Strom 《Genetics in medicine》2006,8(6):339-345
PURPOSE: The study's purpose was to understand the molecular basis for different clinical phenotypes of the 5T variant, a tract of 5 thymidines in intron 8 of the cystic fibrosis transmembrane conductance regulator (CFTR) gene, which disrupts processing of CFTR mRNA and reduces synthesis from the corresponding CFTR alleles. METHOD: We analyzed the polymorphic TG dinucleotide repeat adjacent to the 5T variant in intron 8 and the codon 470 in exon 10. Patients selected for this study were positive for both the 5T variant and the major cystic fibrosis mutation, Delta F508. Almost all Delta F508 mutation alleles occur in a 10TG-9T-470M haplotype. Therefore, it is possible to determine the haplotype of the 5T variant in trans. RESULTS: Of the 74 samples analyzed, 41 (55%) were 11TG-5T-470M, 31 (42%) were 12TG-5T-470V, and 2 (3%) were 13TG-5T-470M. Of the 49 cases for which we had clinical information, 17.6% of females (6/34) and 66.7% of males (10/15) showed symptoms resembling atypical cystic fibrosis. The haplotype with the highest penetrance in females (42% or 5/12) and more than 80% (5/6) in males is 12TG-5T-470V. We also evaluated 12 males affected with congenital bilateral absence of vas deferens and positive for the 5T variant; 10 of 12 had the 12TG-5T-470V haplotype. CONCLUSION: Overall, the 5T variant has a milder clinical consequence than previously estimated in females. The clinical presentations of the 5T variant are associated with the 5T-12TG-470M haplotype. 相似文献