吉西他滨对Beclinl基因沉默的人胰腺癌MiaPaCa-2细胞的周期及凋亡的影响

目的 探讨吉西他滨对Beclinl基因沉默的人胰腺癌MiaPaCa-2细胞周期及凋亡的影响.方法 构建靶向Beclinl的siRNA,插入表达质粒,转染MiaPaCa-2细胞.采用RT-PCR法和蛋白质印迹法检测细胞Beclinl mRNA及蛋白的表达,应用吉西他滨处理Beclinl基因沉默的MiaPaCa-2细胞,应用流式细胞仪检测细胞周期及细胞凋亡状况.结果 成功获得Beclin1基因沉默的MiaPaCa-2细胞,转染后细胞的Beclin1 mRNA表达量从对照组的1.0下降到0.295;S、G2期细胞数减少,而G1期细胞增多;细胞凋亡未受影响.应用吉西他滨处理后,Beclin1基因沉默的MiaPaCa-2细胞的S期细胞数进一步减少,而G1、G2期细胞增多,细胞凋亡被抑制.结论 Beclinl表达沉默使人胰腺癌细胞系MiaPaCa-2细胞周期发生改变,并影响吉西他滨对细胞周期及凋亡的作用.     

Recombinant Lactococcus Expressing a Novel Variant of Infectious Bursal Disease Virus VP2 Protein Can Induce Unique Specific Neutralizing Antibodies in Chickens and Provide Complete Protection

Recent reports of infectious bursal disease virus (IBDV) infections in China, Japan, and North America have indicated the presence of variant, and the current conventional IBDV vaccine cannot completely protect against variant IBDV. In this study, we constructed recombinant Lactococcus lactis (r-L. lactis) expressing a novel variant of IBDV VP2 (avVP2) protein along with the Salmonella resistance to complement killing (RCK) protein, and Western blotting analysis confirmed that r-L. lactis successfully expressed avVP2-RCK fusion protein. We immunized chickens with this vaccine and subsequently challenged them with the very virulent IBDV (vvIBDV) and a novel variant wild IBDV (avIBDV) to evaluate the immune effect of the vaccine. The results show that the r-L. lactis-avVP2-RCK-immunized group exhibited a 100% protection rate when challenged with avIBDV and 100% survival rate to vvIBDV. Furthermore, this immunization resulted in the production of unique neutralizing antibodies that cannot be detected by conventional ELISA. These results indicate that r-L. lactis-avVP2-RCK is a promising candidate vaccine against IBDV infections, which can produce unique neutralizing antibodies that cannot be produced by other vaccines and protect against IBDV infection, especially against the variant strain.     

Molecular cloning and characterization of lactate dehydrogenase gene from Eimeria tenella

Lactate dehydrogenase (LDH) is a key enzyme in the glycolytic pathway and is crucial for parasite survival. In this study, we cloned and expressed the LDH of Eimeria tenella (EtLDH). Real-time polymerase chain reaction and Western blot analysis revealed that the expression of EtLDH was developmentally regulated at the messenger RNA (mRNA) and protein levels. EtLDH mRNA levels were higher in second-generation merozoites than in other developmental stages (unsporulated oocysts, sporulated oocysts, and sporozoites). EtLDH protein expression levels were most prominent in second-generation merozoites, moderately expressed in unsporulated oocysts and sporulated oocysts, and weakly detected in sporozoites. Immunostaining with anti-recombinant EtLDH (rEtLDH) antibody indicated that EtLDH was mainly located in the anterior region in free sporozoites and became concentrated in the anterior region of intracellular sporozoites except for the apex after invasion into DF-1 cells. Specific staining of EtLDH protein was more intense in trophozoites and immature first-generation schizonts, but decreased in mature first-generation schizonts. Inhibition of EtLDH function using specific antibodies cannot efficiently reduce the ability of E. tenella sporozoites to invade host cells. These results suggest that EtLDH may be involved in glycolysis during the first-generation merogony stage in E. tenella and has little role in host invasion.     

八年制临床医学专业大学英语教学现状评估及改革展望：以四川大学为例

目的　探究当前八年制临床医学专业通识教育阶段大学英语课程实施现状及存在的问题,并结合现状提出改进措施。方法　通过问卷分析及访谈的方式对四川大学华西医学中心从事八年制双语课程教学教师、四川大学外国语学院从事八年制大学英语课程教学教师及部分教务管理教师进行调研,分析教学中存在的问题及探索改进措施。结果　学生对大学英语课程重视程度较高,但任课教师反映学生的参与度较低。整体上,大学英语课程教学效率较低,亟待改进。结论　八年制大学英语课程实施效率不理想,原因来自教师、学生双方,需从两方面进行改进。     

金银花配方颗粒评价性抽验结果分析与建议

目的：通过开展金银花配方颗粒评价性抽验,比较分析不同厂家金银花配方颗粒的质量情况,并提出监管建议。方法：收集各企业金银花配方颗粒质量标准,依据企业标准进行法定检验。结果：共抽验117批金银花配方颗粒,涉及9家生产企业。按各自企业标准进行检验,总体合格率为100%。但是各企业之间金银花配方颗粒含量差异较大且每克颗粒相当的药材（饮片）量不同,转移率不同。结论：通过本次抽验发现金银花配方颗粒的整体质量较高,但是不同厂家之间所用原料药材及生产工艺存在一定差异,导致最终产品质量参差不齐。因此,有必要规范中药配方颗粒生产工艺,尽快统一标准,加强监管。