An animal model of SARS produced by infection of Macaca mulatta with SARS coronavirus

A new SARS animal model was established by inoculating SARS coronavirus (SARS-CoV) into rhesus macaques (Macaca mulatta) through the nasal cavity. Pathological pulmonary changes were successively detected on days 5-60 after virus inoculation. All eight animals showed a transient fever 2-3 days after inoculation. Immunological, molecular biological, and pathological studies support the establishment of this SARS animal model. Firstly, SARS-CoV-specific IgGs were detected in the sera of macaques from 11 to 60 days after inoculation. Secondly, SARS-CoV RNA could be detected in pharyngeal swab samples using nested RT-PCR in all infected animals from 5 days after virus inoculation. Finally, histopathological changes of interstitial pneumonia were found in the lungs during the 60 days after viral inoculation: these changes were less marked at later time points, indicating that an active healing process together with resolution of an acute inflammatory response was taking place in these animals. This animal model should provide insight into the mechanisms of SARS-CoV-related pulmonary disease and greatly facilitate the development of vaccines and therapeutics against SARS.     

Windows环境下步进电机匀加速的实现与计算机程序设计

本文主要介绍了奔腾计算机和步进电机的接口电路,提出了步进电机匀加速的控制算法,探讨了在 Windows 环境下以 Visual C++实现步进电机匀加速的实现途径和多任务环境下匀加速程序的设计方法。     

Functional polymorphisms in cell death pathway genes FAS and FASL contribute to risk of lung cancer

Background: The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis. There is reduced expression of FAS but elevated expression of FASL in many types of human cancers including lung cancer. We recently reported an association between functional polymorphisms in FAS (–1377G→A) and FASL (–844T→C) and risk of oesophageal cancer.     

血糖感应型胰岛素给药智能载体的研究进展

胰岛素控制释放高分子载体系统一直是国内外科技工作者的研究热点 ,迄今已经研究报道了多种具有不同工作原理的血糖感应型胰岛素给药智能载体。本文基于国内外大量研究文献 ,综述了血糖感应型胰岛素控制释放智能化高分子载体的研究进展。     

缺血预处理对大鼠骨骼肌缺血再灌注损伤时Bax基因蛋白表达影响的实验研究

探讨缺血预处理对大鼠骨骼肌缺血再灌注损伤的可能影响,特别是对Bax基因表达的影响.用免疫组化方法检测并观察大鼠后肢止血带缺血4 h后再灌注4、24、48 h后和先经预缺血处理后再灌注4、24 h后Bax基因蛋白在肌细胞中的表达情况.结果表明缺血4 h(A组)肌细胞出现Bax蛋白表达,24h后(B组)表达量显增加,48h后(C组)无表达.缺血预处理后再灌注4 h后(D组)肌细胞内无Bax蛋白表达,再灌注24 h后(E组)Bax蛋白表达量非常显著地低于B组.Bax基因参与了骨骼缺血再灌注损伤的病理过程,其蛋白表达量与组织损伤程度密切相关,经缺血预处理可以下调Bax基因蛋白的表达水平、或是抑制其合成,使肌细胞存活而发挥其保护作用.     

电刺激杏仁核对猫丘脑后核内脏大神经诱发放电的抑制作用

本文使用条件—检验刺激技术,研究了电刺激杏仁皮质内侧核对猫丘脑后核内脏大神经诱发放电的影响。电刺激杏仁皮质内侧核抑制丘脑后核的中等和长潜伏期内脏大神经诱发放电呈现两种形式:即时抑制和迟发抑制。前者可能是通过从杏仁核到丘脑后核的神经通路产生的,静脉注射纳洛酮可消除这种抑制,提示有鸦片样物质的参与。后者可能涉及神经—体液性因素。结果提示,杏仁核参与抑制内脏痛。     

乳酸及降钙素原对一氧化碳中毒后迟发性脑病的预测价值

目的探讨检测血乳酸、降钙素原和联合检测对急性一氧化碳中毒后迟发性脑病(DEACMP)的预测价值。 方法随机抽取我院在急诊科收住院的ACMP患者70例,随访3个月,以其中发生DEACMP患者10例为DEACMP组,其余60例为非DEACMP组,随机抽取同期笔者医院40例健康体检者为对照组。对患者立即行动脉血Lac和血清PCT检测,并计算Lac和PCT诊断DEACMP的灵敏度、特异度。 结果DEACMP组血Lac和PCT检测值均明显高于非DEACMP和对照组,差异有统计学意义(采用秩和检验,P<0.05);检测异常率亦明显高于非DEACMP组及对照组,差异有统计学意义(采用χ²检验,P<0.05)。Lac和PCT升高预测DEACMP的敏感度均为90.0% ,特异度分别为50.0%和65.0% ,准确性分别为55.7%和68.6%。联合检测时若其中1个指标为阳性时即预测DEACMP发病,则联合检测的敏感度为100.0%,特异度为41.7%,阳性预测值为21.7%,阴性预测值为100.0%,准确性为71.4%。 结论DEACMP患者早期动脉血Lac和PCT均明显升高,PCT诊断价值优于Lac,联合检测较单项指标检测对DEACMP发病预测价值更理想。     

益气止血药对脾不统血证模型动物前列腺素的作用

采用复合因素的脾不统血证大鼠模型 ,以 6 酮 前列腺素F1α(6 酮 PGF1α)和血栓素B2(TXB2 )作为观测指标 ,对益气止血药对脾不统血证模型大鼠血浆和子宫前列腺素的影响进行了研究。结果 :大剂量组益气止血药能降低脾不统血证模型血浆和子宫的 6 酮 PGF1α及子宫 6 酮 PGF1α/TXB2 比值。说明 6 酮 PGF1α及 6 酮 PGF1α/TXB2 比值升高可能是脾不统血证的重要机理之一。     

Cloning and identification of a novel variant of human vascular endothelial growth factor

Vascular endothelialgrowth factor ( VEGF) is amultifunctional cytokine thatexerts in vivo a key rolein physiologicalneoangiogenesisduring embryonicde-velopmentor the female cycle and pathologicalneoan-giogenesis of many diseases including hypervascular-ized tumors,rheumatoid arthritis,and retinopathiesdiseases[1— 3] by stimulating endothelial cell prolifera-tion and vessel hyperpermeability.VEGF exists asone of five different isoforms,VEGF1 2 1 ,VEGF1 65 ,VEGF1 83,VEGF1 89and VEGF2 …     

还原型谷胱甘肽治疗尿毒症贫血的初步探讨

目的　观察外源性还原型谷胱甘肽对尿毒症贫血的治疗效果。方法　将 42例尿毒症贫血患者随机分为两组。两组患者均给予重组人红细胞生成素 (r- Hu EPO) 30 0 0 U,每周两次皮下注射 ,疗程 12周。治疗组患者同时静脉给予外源性还原型谷胱甘肽 (GSH) 12 0 0 mg,每周两次 ,疗程 12周。于治疗前和治疗后抽静脉血测定血红蛋白 (Hb) ,红细胞 (RBC)和红细胞压积 (HCT)水平。结果　 r- Hu EPO治疗后两组患者 HB,RBC和 HCT水平均显著上升 (P<0 .0 1)。 GSH治疗组 Hb,RBC和 HCT水平与对照组比较上升明显 (P<0 .0 5 ) ,治疗组 Hb,RBC和HCT的上升幅度高于对照组 (P<0 .0 5 )。结论　外源性还原型谷胱甘肽能显著提高 r- Hu EPO治疗肾性贫血的疗效 ,GSH可能是治疗尿毒症贫血的有效药物。