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991.
The spike (S) gene of the attenuated porcine epidemic diarrhea virus (PEDV) DR13 was cloned and sequenced to further explore
the functions of wild type PEDV and attenuated PEDV. Sequencing revealed a single large ORF of 4,149 nucleotides encoding
a protein of 1,382 amino acids with predicted M
r of 151 kDa. The coding region of the S gene of attenuated PEDV DR13 had 20 nucleotide changes that appeared to be significant
determinants of function in that they produced changes in its predicted amino acid sequence. Notably, attenuated PEDV DR13
has previously been found to exhibit reduced pathogenicity in pigs. The regions containing these 20 nucleotide changes may
therefore be crucial for PEDV pathogenicity. The attenuated PEDV DR13 S protein contains 28 Asn-Xaa-Ser/Thr sequons, 21 asparagines
that are predicted to be N-glycosylated and a stretch of highly hydrophobic residues at positions 1,327–1,347, which is predicted
to form an α-helix and to function as a membrane anchor. One (from N to K at 378) of the changes in the deduced amino acid
sequence destroyed N-linked glycosylation sites, while another change (from N to S at 114) created a new one at a different
location. These alterations in N-linked glycosylation sites reflected 3 nucleotide changes, which were related to the above-mentioned
nucleotide changes and are suggested to influence the pathogenicity of attenuated PEDV DR13. Attenuated PEDV DR13 has 96.5,
96.4, 96.1, 93.9, 93.5 and 96.6% DNA sequence identities with CV777, Br1/87, JS-2004-2, Spk1, Chinju99 and parent DR13, respectively.
Likewise, it shares 95.7, 95.4, 95.6, 92.0, 91.6 and 95.7% identity with those genes at the deduced amino acid sequence level.
Phylogenetic analysis suggested that attenuated PEDV DR13 is closely related to CV777, Br1/87, JS-2004-2 and parent DR13,
rather than to Spk1 and Chinju99 and is especially close to the Chinese PEDV strain JS-2004-2.
Nucleotide sequence data reported is available in the GenBank database under the Accession Nos. DQ462404 and DQ862099. 相似文献
992.
Anh Bui Thi Kim Minh Nguyen Ngoc Ha Nguyen Thi Hoang Kim Dang Dinh Kien Nguyen Trung Trung Nguyen Quang Cuong Tran Thien Danh Luu Thai 《Bulletin of environmental contamination and toxicology》2018,100(5):720-726
Bulletin of Environmental Contamination and Toxicology - In field survey, Pteris vittata and Pityrogramma calomelanos were only found in arsenic (As) contaminated areas with soil pH 7.2–8.8... 相似文献
993.
Choi KS Jeon HO Lee YS Jang YL Kim CH Ha KS Shin MH Hong KS 《Psychiatric genetics》2007,17(2):103-107
OBJECTIVE: The objective of this study was to characterize the familial associations of symptoms or symptom dimensions of schizophrenia, as a component of the effort to delineate suitable phenotypic markers under the homogenous genetic control. METHODS: Clinical symptoms of the patients were evaluated on a lifetime basis, with the Korean version of the Diagnostic Interview for Genetic Studies and the Krawieka scale. Our sample consisted of 54 sibling pairs who were concordant for Diagnostic and statistical manual of mental disorder-IV schizophrenia or schizoaffective disorder. RESULTS: At the individual symptom level measured by the Diagnostic Interview for Genetic Studies, we observed a statistically significant degree of concordance within the sibling pairs for auditory hallucination (kappa=0.600, chi(2)=15.193, P=0.000). The within-sibling resemblance of this symptom was also indicated by the results of correlation analyses of individual items of the Krawiecka scale (partial correlation coefficient=0.423, P=0.005). Among the individual symptoms or symptom dimensions of the Krawiecka scale, the 'anxious' (partial correlation coefficient=0.469, P=0.001) and the 'affective dimension' (including the items of 'anxious' and 'depressed') (partial correlation coefficient=0.436, P=0.003) were also determined to indicate significant intra-pair correlations after controlling for potential confounding variables, such as sex, duration of illness, and age of onset. CONCLUSIONS: The results of this study suggest that familial factors, possibly genetic factors, contribute a liability to the development of auditory hallucinations. This symptom might then prove useful as a phenotype marker in future genetic studies. 相似文献
994.
Kim OJ Ha EH Kim BM Seo JH Park HS Jung WJ Lee BE Suh YJ Kim YJ Lee JT Kim H Hong YC 《Journal of occupational and environmental medicine / American College of Occupational and Environmental Medicine》2007,49(12):1394-1402
OBJECTIVE:: The aim of this study was to evaluate the effects of PM10 on birth outcomes using a prospective cohort of pregnant women. METHODS:: The multicenter prospective study was conducted in Korea from 2001 to 2004. To estimate the effects of PM10 exposure on birth outcomes, the logistic and linear regression model and the generalized additive model for nonlinear relationships were used. RESULTS:: Stillbirths were affected by PM10 level during the third trimesters (OR = 1.10, 95% CI = 1.02-1.14), and birth defects were influenced by the PM10 exposure during the second trimesters (OR = 1.16, 95% CI = 1.00-1.34). Intrauterine growth retardation was affected by the first trimester's PM10 exposure. On the other hand, premature birth was affected by the PM10 exposure during the third trimester, and low-birth-weight births were affected by the PM10 level during entire trimesters of pregnancy. CONCLUSIONS:: PM10 exposure during pregnancy may result in adverse birth outcomes with different critical periods. 相似文献
995.
Khan NC Ninh NX Van Nhien N Khoi HH West CE Hautvast JG 《Asia Pacific journal of clinical nutrition》2007,16(1):152-157
The objective of the study was to assess the prevalence of sub clinical vitamin A deficiency and anemia in Vietnamese children. For this, a cross-sectional survey was conducted in 40 villages (clusters) of four ecological regions in Vietnam during Apr-May 2001. In total 1657 children less than 5 years old were included by a cluster random sampling method. The prevalence of sub clinical vitamin A deficiency (serum retinol <0.70 mumol/l) was 12.0% and the prevalence of anemia (hemoglobin <110g/l) was 28.4 %. 35.1%. In the children under 6 months the prevalence of sub clinical vitamin A deficiency was 35.1 % whereas the prevalence of anemia in this group was as high as 61.7%. The prevalence of children with both sub clinical vitamin A deficiency and anemia was 6.1%. Sub clinical vitamin A deficiency and anemia prevalence differed significantly across the regions, with highest prevalence in the Northern Mountainous areas for vitamin A deficiency and in the Northern Mountainous area and Mekong River Delta for anemia. It is concluded that sub clinical vitamin A deficiency and anemia are still important public health problems in Vietnam. Sustainable strategies for combating vitamin A deficiency and nutritional anemia are needed and should concentrate on target groups, especially infants and malnourished children in high risk regions. 相似文献
996.
Ae Ryang Jung Ga Eun Kim Mee Young Kim U-Syn Ha Sung-Hoo Hong Ji Youl Lee Sae Woong Kim Yong Hyun Park 《American journal of cancer research》2021,11(5):2215
Prostate cancer (PCa) is the most common male cancer. Most patients treated with androgen deprivation therapy progress to castration-resistant PCa. To overcome the limitations of this treatment, there is an urgent need to identify more effective treatment targets. High mobility group box 1 protein (HMGB1) is known to be associated with progression, metastasis, and poor prognosis of several solid tumors; however, its role in PCa remains unclear. Thus, we aimed to evaluate the clinical significance and biological roles and mechanism of HMGB1 in PCa. We showed that increased expression of HMGB1 correlated with increased risk of aggressive PCa, and high expression of HMGB1 was associated with poor biochemical recurrence-free survival in a Korean cohort. Additionally, the inhibition of HMGB1 expression significantly reduced cell proliferation, invasive capacity, and NF-κB signaling in vitro. Our results indicated that HMGB1 is a critical factor in the development and progression of PCa. Moreover, we found that HMGB1 directly interacts with TNFR1, and TNFR1 overexpression in HMGB1 knockdown cells reversed the effects of HMGB1 knockdown. Importantly, our results suggest that HMGB1 binding to TNFR1 promotes tumor progression by activating the NF-κB signaling pathway in PCa; therefore, the HMGB1/TNFR1/NF-κB signaling pathway could serve as a novel therapeutic target for improving PCa therapy. 相似文献
997.
Association between Early Menopause,Gynecological Cancer,and Tobacco Smoking: A Cross-Sectional Study 下载免费PDF全文
Joyce Mary Kim Yeun Soo Yang Su Hyun Lee Sun Ha Jee 《Asian Pacific journal of cancer prevention》2021,22(10):3165-3170
Background: The rates of smoking among women are rising. Previous studies have shown that smoking is associated with early menopause. However, the association of gynecological cancer, including breast and cervical cancer, with early menopause and smoking, remains unclear. Therefore, this study aimed to determine the association between smoking and early menopause, breast cancer, and cervical cancer. Methods: This cross-sectional study used data from the Korean National Health and Nutritional Survey Examination (KHANES) (2016–2018). Early menopause was defined as menopause before 50 years of age. Results: A total of 4,481 participants were included in the analysis. There was no association between early menopause and cervical cancer (adjusted odds ratio [aOR]: 1.435, 95% confidence interval [CI]: 0.730–2.821), but women who had experienced early menopause had a significantly higher risk of breast cancer than women who had experienced normal menopause (aOR: 1.683, 95% CI: 1.089–2.602, p=0.019). Early menopause was not associated with an increased risk of breast cancer in ever-smoker (aOR: 0.475, 95% CI: 0.039–5.748), but was associated with a significantly increased risk of breast cancer in never-smokers (aOR: 1.828, 95% CI: 1.171–2.852). Conclusions: Early menopause was associated with an increased risk of breast cancer in women who had never smoked, but not in women who had ever smoked. 相似文献
998.
Quiñones O Oh JE Vanderford B Kim JH Cho J Snyder SA 《Environmental toxicology and chemistry / SETAC》2007,26(7):1349-1354
The objective of the present study was to conduct a preliminary assessment for perchlorate in surface water, drinking water, and wastewater treatment plant effluent samples obtained from the Nakdong and Yeongsan watersheds in the Republic of Korea. Samples were analyzed for perchlorate using ion chromatography with suppressed conductivity detection (IC-CD) and/or liquid chromatography with tandem mass spectrometry detection (LC-MS/MS). Method reporting limits were 5.0 microg/L for IC-CD and 0.05 microg/L for LC-MS/MS analysis. At perchlorate levels above 5.0 microg/L, IC-CD and LC-MS/MS provided comparable results. The levels of perchlorate detected in the samples procured from the Yeongsan watershed were < 5.0 microg/L in each case. However, Nakdong watershed samples contained perchlorate at levels up to 60 microg/L. The highest concentrations of perchlorate were found in surface water samples, although drinking water contained perchlorate at concentrations up to 35 microg/L. In a subset of samples analyzed by LC-MS/MS, chlorate and bromate also were detected at concentrations ranging from < 0.10 to 44 microg/L and < 0.10 to 2.6 microg/L, respectively. To the best of the authors' knowledge, this is the first perchlorate assessment reported for water sources in the Republic of Korea. 相似文献
999.
Amani Kallel Samir Ben Ali Yosra Sediri Sonia Chabrak Monia Elasmi Ha?fa Sanhaji Omar Souheil Sameh Haj-Taieb Moncef Feki Rachid Mechmeche Riadh Jemaa Naziha Kaabachi 《Clinical chemistry and laboratory medicine》2008,46(8):1097-1101
BACKGROUND: Numerous polymorphisms of the apolipoprotein B (APOB) gene have been described. Particularly, the insertion/deletion (Ins/Del) polymorphism located in the coding part of the signal peptide of apoB, associated with modification of lipid concentrations and the risk of coronary artery disease and/or myocardial infarction (MI), has been reported in the general population. Moreover, conflicting results emerge from the literature and suggest that the effect is context-dependent. In the present study, the first investigation of the Ins/Del polymorphism of the APOB gene in Tunisian patients with MI, we examined a possible association between this polymorphism and MI in a subgroup of the Tunisian population. METHODS: A total of 318 Tunisian patients with MI and 368 healthy controls were included in the study. Genomic DNA was extracted from white blood cells, and the Ins/Del polymorphism was determined by electrophoresis in polyacrylamide gels after PCR amplification. A binary logistic regression analysis was performed to test how the association between MI and Ins/Del polymorphism is independent from confounding factors. RESULTS: A significant difference in genotype distribution and allele frequency was observed between patients and controls. Patients with MI had a frequency of 7.2% for the Del/Del genotype, 39.6% for the Ins/Del genotype, and 53.1% for the Ins/Ins genotype. Controls had a frequency of 3.0% for the Del/Del, 32.1% for the Ins/Del and 64.9% for the Ins/Ins genotype (chi2=12.93, p=0.002). The MI patient group showed a significantly higher frequency of the Del allele compared to controls (27.1% vs. 19.1%; chi2=12.50, p=0.0004). In comparison to the Ins/Ins homozygotes, the odds ratio (95% confidence interval) for MI was 1.51 (1.09-2.07) for Ins/Del heterozygotes and 2.95 (1.40-6.22) for Del/Del homozygotes. In multivariate analysis, age (p=0.001), smoking (p<0.001), hypertension (p=0.001), diabetes mellitus (p<0.001), and dyslipidemia (p=0.01) were independent correlates of the presence of MI, whereas the Ins/Del polymorphism (p=0.330) was not an independent predictor of MI. CONCLUSIONS: The present study shows a significant but not independent association between the Ins/Del polymorphism of the APOB gene and MI in the Tunisian population. 相似文献
1000.
Orthostatic dizziness in Parkinson's disease is attributed to cerebral hypoperfusion: A transcranial doppler study 下载免费PDF全文