QS Alex 755 nm激光对黑素细胞p16INK4a表达的影响

目的 了解激光对黑素细胞潜在恶变作用的可能性。方法 QS Alex 755nm激光体外照射HTB66、Sk-mel-24和G361 3种黑素瘤细胞株,照射剂量为0．85～2 J／cm^2,24h后收集细胞。应用流式细胞仪和RT-PCR方法分别测定p16INK4a蛋白和p16INK4a mRNA在激光照射前后和不同剂量照射后的表达水平。结果激光照射后,HTB 66细胞株(p16INK4a蛋白阳性株)的p16INK4a蛋白表达明显增加：而低水平表达的Sk-mel-24和G361细胞株无明显变化。在HTB66细胞株中虽然p16INK4a蛋白的表达上调,但对其功能的测定发现它并不能阻止细胞周期从G0/G1向S／G2M转变。HTB66细胞株的p16INK4a mRNA的表达也增加。结论经研究剂量的激光照射后DNA有损伤。具有黑素瘤家族史或个人史的患者可能有p16INK4a基因的突变或丢失,不提倡用激光治疗此类患者的色素性疾病。     

Histology as a diagnostic tool for intestinal isosporiasis in immunocompromised patients

Isospora belli is an opportunistic protozoan causing wasting diarrhea especially in patients with an immunocompromised status. Diagnosis is usually established by demonstrating the oocyst of the organism on stool examination, which however can often be inconclusive. Serological tests for isosporiasis are currently not available. In such a scenario, biopsy often provides evidence for a confirmatory diagnosis. We describe two such cases, in which intestinal biopsy was the only diagnostic evidence of isosporiasis as the cause for chronic diarrhea.     

香港地区肿瘤放射治疗发展简史

通过回顾香港地区肿瘤放射治疗设备、人员、技术等情况的进展,可为广大读者提供一个简单清晰的脉络,并帮助了解香港地区肿瘤放射治疗学创建与发展过程。此报告是对香港地区肿瘤放射治疗学发展的宣传与肯定,也更是为扩大放射肿瘤学科交流和发展提供契机。     

The Hutson loop and prosthesis: Clinical uses in hepato-biliary intervention

A number of method of making a Roux-en-Y hepaticojejunostomy have been advocated to help the radiologist gain access to the biliary system. We have found markers and rings unsatisfactory and therefore designed and tested our own prosthesis. Reliable rapid access is easily achieved using this device with a much reduced radiation dose to both patient and radiologist. The prosthesis has been used for the past 3 years, and the last 23 patients are reviewed. Seven patients have required repeated interventions via the loop and we present the findings and our results.