Gallium-67 lung uptake: conjugate-view technique

A conjugate-view technique is derived for calculation of absolute gallium-67 (Ga-67) uptake from scintillation-camera images. The technique combines counts of posterior and anterior images of the lung with an attenuation correction obtained from cobalt-57 (Co-57) transmission imaging. The formulation is such that the effects of Compton scatter build-up are accounted for. Studies utilizing a canine model indicated that, normally, more activity is located in the chest wall than in the lungs. The quantitative technique must therefore accurately account for a variety of Ga-67 distributions, including that in the chest wall. Calculations were performed using a three-component model comparing results obtained with the conjugate-view approach to the actual uptake. These calculations suggest that an assumption of uniform activity distribution allows an accuracy of approximately +/- 10% over a broad range of body-part thicknesses and uptake by the lungs. It was concluded that the conjugate-view technique is necessarily approximate but can provide clinically useful results.     

Platelet Functions in Cardiopulmonary Bypass Surgery

Background

Haemorrhage after Cardio Pulmonary Bypass (CPB) Surgery is a well recognised complication that leads to significant morbidity and mortality. The incidence varies between 5-25% depending upon the clinical situation. Several factors are implicated as causative but none have been precisely proved.

Methods

Our study was an attempt to evaluate the haemostatic defect with particular reference to platelet function abnormalities during cardio pulmonary bypass surgery, in order to reduce the morbidity and mortality associated with post CPB haemorrhage. Flow cytometric evaluation of different platelet glycoproteins like GPIb/IX, GPIIb/IIIa and GMP-140 was done.

Results

The marker expression showed deregulation during surgery which returned to base after bypass was terminated. In contrast, the cases with bleeding showed significant variation. P-Selectin (GMP 140) expression decreased progressively till 3^rd post-operative day showing lack of activation of platelets in cases of severe bleeding.

Conclusion

Longer duration of CPB initiates plasmin generation through heparin, which raises the PAI-1-tPA complex and thereby down regulating the functions of platelets. This suggests a link between duration of CPB, bleeding, platelet dysfunction and fibrinolysis. Hence serial estimations of the levels of GMP-140 and tPA can predict severe bleeding.Key Words: CardioPulmonary Bypass, Platelet dysfunction, flowcytometry, platelet glycoproteins, haemorrhage     

Acquisition and retrieval of information in amphetamine-treated hyperactive children

State-dependent learning and memory (retrieval) processes were examined in 15 amphetamine-treated hyperactive boys. While stimulant treatment enhanced the acquisition of information and its retrieval 24 hours later, there was no evidence of poorer retrieval of information learned in a state different from the retrieval state. Amphetamine appeared particularly to facilitate effortful cognitive processes. Subgroups of hyperactive children respond to amphetamine treatment in different ways, some showing changes in motor restlessness and others changes in cognition. The lack of dissociative effects when information is learned and recalled under different drug conditions suggests that what the stimulant-treated child learns can be effectively recovered after completion of treatment.     

A Finite Volume Method for Modeling Discontinuous Electrical Activation in Cardiac Tissue

This paper describes a finite volume method for modeling electrical activation in a sample of cardiac tissue using the bidomain equations. Microstructural features to the level of cleavage planes between sheets of myocardial fibers in the tissue are explicitly represented. The key features of this implementation compared to previous modeling are that it represents physical discontinuities without the implicit removal of intracellular volume and it generates linear systems of equations that are computationally efficient to construct and solve. Results obtained using this method highlight how the understanding of discontinuous activation in cardiac tissue can form a basis for better understanding defibrillation processes and experimental recordings.     

The influence of unequal numbers of trials on comparisons of average event-related potentials

Four studies examined (a) how event-related potentials (ERPs) change as the number of trials averaged increases and (b) the statistical implications of comparing ERPs composed of different numbers of trials. Experiment 1 utilized data from 7-year-old children performing an oddball task. The other three experiments used simulated data with different distributions of P3 peak latency. In all 4 experiments, peak amplitude decreased and the mean amplitude of the 300 to 900 msec interval remained stable as the number of trials averaged increased. The standard deviations of both measures decreased. These data show that the decrease in peak amplitude with increasing numbers of trials that has been found in other studies is not solely due to the elimination of residual noise but is likely to also involve a fundamental aspect of signal averaging and the algorithm used to select peaks. Furthermore, these experiments expose the possibility of statistical errors when investigators compare average ERPs composed of small versus large numbers of trials as is often done when the oddball paradigm is used.     

Isolation of two phenylethanoid glycosides from Eremophila gilesii

The leaves of Eremophila gilesii have been used traditionally to treat colds, headaches, sores, and chest pains. Our previous screening of Australian native plants showed that the methanol extract of the aerial parts of E. gilesii demonstrated notable inhibition of ADP-induced human platelet aggregation and serotonin release. Subsequent fractionation on the methanol extract led to the isolation of two phenylethanoid glycosides, verbascoside (1) and poliumoside (2). This is the first study reporting the presence of phenylethanoid glycosides in E. gilesii.