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排序方式: 共有944条查询结果,搜索用时 15 毫秒
931.
Online health information seeking: how people with multiple sclerosis find,assess and integrate treatment information to manage their health 下载免费PDF全文
Anneliese J. Synnot B. Physio MPH Sophie J. Hill BA MA PhD Kerryn A. Garner B Soc Sci MA Michael P. Summers PhD MSocPol BAppSocSci Graziella Filippini MD Richard H. Osborne Assoc Dip Appl Biol BSci PhD Sue D.P. Shapland RN BN MSCN Cert MS Nursing Cinzia Colombo D Phil Paola Mosconi Biol Sci D 《Health expectations》2016,19(3):727-737
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Betterle C Coco G Zanchetta R 《Best Practice & Research: Clinical Endocrinology & Metabolism》2005,19(1):85-99
The recent advances in our understanding of immunology have greatly improved our knowledge about the natural history of autoimmune diseases and, in particular, of autoimmune Addison's disease (Autoimmune AD). Autoimmune AD is a chronic disorder with a long preclinical period marked by the presence of adrenal cortex autoantibodies (ACAs). In this chapter the main data on this will be analyzed. The populations with the highest risk of Autoimmune AD are first relatives of patients with AAD and patients with autoimmune diseases, particularly those with chronic hypoparathyroidism or with premature ovarian failure. The best markers to identify the subjects at risk are ACAs detected by the immunofluorescence test on human or animal tissues, or 21-hydroxylase autoantibodies (21-OHAbs) detected by radioimmunoassay (RIA). The evaluation of adrenal cortex function in these individuals includes the basal determination of adrenocorticotropic hormone (ACTH), cortisol, aldosterone, plasma renin activity and cortisol after intravenous stimulation with synthetic ACTH. The multivariate analysis of the main factors (genetics, age, gender, titers of antibodies, pre-existing disease, status of the adrenal function) revealed that the risk of future AAD depends only on the presence of high antibody titers, chronic hypoparathyroidism or chronic candidiasis and adrenal dysfunction. On the basis of these parameters the risk of future Autoimmune AD can be calculated with an equation model. Patients with different risk scores need to be monitored at different time intervals, and those at high risk need to be strictly monitored and are the ideal subjects for future prevention trials. 相似文献
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Madeddu G Calia GM Campus ML Lovigu C Mannazzu M Olmeo P Mela MG Mura MS 《International journal of STD & AIDS》2008,19(9):644-645
The availability of antiretroviral therapy has dramatically reduced the risk of HIV mother-to-child transmission (MTCT). However, mothers infected with multidrug resistant HIV (MDR-HIV) are at increased risk of MTCT. We report the case of a pregnant patient infected with MDR-HIV in whom MTCT was avoided with enfuvirtide use in late pregnancy and elective caesarean section. 相似文献
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Evaluation of a new line probe assay for rapid identification of gyrA mutations in Mycobacterium tuberculosis 总被引:1,自引:0,他引:1 下载免费PDF全文
Giannoni F Iona E Sementilli F Brunori L Pardini M Migliori GB Orefici G Fattorini L 《Antimicrobial agents and chemotherapy》2005,49(7):2928-2933
Resistance of Mycobacterium tuberculosis to fluoroquinolones (FQ) results mostly from mutations in the gyrA gene. We developed a reverse hybridization-based line probe assay in which oligonucleotide probes carrying the wild-type gyrA sequence, a serine-to-threonine (S95T) polymorphism, and gyrA mutations (A90V, A90V-S95T, S91P, S91P-S95T, D94A, D94N, D94G-S95T, D94H-S95T) were immobilized on nitrocellulose strips and hybridized with digoxigenin-labeled PCR products obtained from M. tuberculosis strains. When a mutated PCR product was used, hybridization occurred to the corresponding mutated probe but not to the wild-type probe. A panel of M. tuberculosis complex strains including 19 ofloxacin-resistant (OFL-R) and 9 ofloxacin-susceptible (OFL-S) M. tuberculosis strains was studied for detection and identification of gyrA mutations by the line probe assay and nucleotide sequencing, in comparison with testing of in vitro susceptibility to FQ. Results were 100% concordant with those of nucleotide sequencing. The S95T polymorphism, which is not related to FQ resistance, was found in 5 OFL-S and 2 OFL-R strains; the other 17 OFL-R strains harbored single mutations associated with serine or threonine at codon 95. No mutations were found in the other OFL-S strains. Overall, on the basis of the MICs on solid medium, the new line probe assay correctly identified all OFL-S and 17 out of 19 (89.5%) OFL-R strains. A nested-PCR protocol was also evaluated for the assay to amplify PCR products from M. tuberculosis-spiked sputa, with a good specificity and a sensitivity of 2 x 10(3) M. tuberculosis CFU per ml of sputum. 相似文献
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Individually rare, when taken as a whole, genetic inborn errors of metabolism (IEM) account for a significant proportion of early onset encephalopathy. Prompt diagnosis is crucial to assess appropriate investigation and can sometimes warrant successful therapy. Recent improvements in technology and expansion of knowledge on the biochemical and molecular basis of these disorders allow astute child neurologists and paediatricians to improve the early diagnosis of these genetically determined defects. However, because of rarity and heterogeneity of these disorders, IEM encephalopathies are still a formidable challenge for most physicians. The most frequent cause of childhood IEM encephalopathy is mitochondrial disease, whose biochemical 'signature' is faulty energy supply due to defects of the last component of the oxidative pathways residing within mitochondria, i.e. the mitochondrial respiratory chain. 相似文献
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Daniele?Clementi Riccardo?D’AmbrosiEmail authorView authors OrcID profile Paride?Bertocco Miguel?Simon?Bucci Carlo?Cardile Paolo?Ragni Graziella?Giaffreda Vincenza?Ragone 《European journal of orthopaedic surgery & traumatology : orthopedie traumatologie》2018,28(5):915-922