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??Abstract??Objective To explore the role of miR-125b in pediatric classical APL?? in order to seek new therapeutic strategies for drug resistant APL. Methods The target genes of miR-125b were predicted online?? validated by Dual-luciferase assay and western blot assay. MiR-125b expression levels were measured in 33 matched-pair APL samples??treated in the First Affiliated Hospital of Sun Yat-sen University and other members of South China Children APL Cooperative Group from March 2007 to September2012??at initial diagnosis and complete remission ??CR?? and in 5 relapsed patients by qRT-PCR. Proliferation and apoptosis were analyzed respectively using the RNA transfection?? MTT assay and flow cytometry. Results The expression of miR-125b was up-regulated in pediatric APL at diagnosis and relapse bone marrow samples?? but returned to normal after complete remission?? miR-125b could promote leukemic cell proliferation and inhibit cell apoptosis by regulating the expression of tumor suppressor Bak1. Remarkably??it was also found to be up-regulated in leukemic drug-resistant cells??NB4-R1??NB4-R2??HL-60/DOX???? and overexpression of exogenous miR-125b could increase their resistance to therapeutic drugs. Conclusion MiR-125b can regulate pediatric classical APL cells proliferation?? apoptosis and drug resistance by repressing BAK1 protein expression.     

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??Abstract??The fifth edition of Diagnostic and Statistical Manual of Mental Disorders ??DSM-???? is released in May 2013.The new term of autism spectrum disorder ??ASD?? replaced the term of pervasive developmental disorder ??PDD??.In this paper??the background for the current definition of ASD??the major changes of DSM-?? in ASD??the possible effects on the clinical practice of diagnosis and treatment of autism??and the continuous debate on this topic will be reviewed.     

Practical guide to the diagnosis of thalassemia

Thalassemias occur in individuals of all ethnic backgrounds and are among the most common genetic diseases worldwide. The diagnosis of thalassemia can easily be part of primary medical practice. Here we outline a practical approach to the detection of thalassemias in three common clinical settings. The first involves any patient with a low mean corpuscular volume (MCV) with or without anemia. The second is a neonatal screening result indicating possible presence of thalassemia. Finally, evaluation for thalassemia should be considered in the context of family planning or pregnancy in patients whose ethnicity indicates origin from high risk geographic areas. We also review the various types of the thalassemia syndromes and provide an overview of general therapeutic considerations. © 1996 Wiley-Liss, Inc.     

细胞焦亡对肝癌发生发展的影响及中医药的干预作用

细胞焦亡是一种新发现的程序性细胞死亡方式,在肝癌中发挥着关键作用。故拟基于细胞焦亡的分子机制及其在肝癌发病机制中的作用进行深入分析,并结合中医药在调节焦亡肝癌发展过程中的作用进行探讨,以期为临床提供新的治疗思路。     

GERD患者自主神经功能与反流症状及食管动力的关系

目的探讨自主神经功能障碍在胃食管反流病(GERD)发病中的作用。方法采用心血管反射检测自主神经的功能状态;根据自主神经功能评价结果,进一步分析自主神经受累程度与食管动力、反流性食管炎的程度及反流症状之间的关系。结果①GERD患者中自主神经功能异常者达59.5%,尤以副交感神经受累为著;②GERD患者中自主神经功能正常组反流症状积分明显低于异常组(P<0.05),自主神经受累的程度与反流症状积分呈显著的正相关(r=0.54,P<0.05);③GERD患者中自主神经异常组食管远端有效收缩率及传导速度明显低于正常组(P<0.05)。结论GERD患者存在明显的自主神经功能异常,而且自主神经功能状态与食管远端的清除能力和反流症状有关,提示自主神经功能障碍在GERD发病中起着一定的作用。     

报刺法联合火针治疗寒湿痹阻型类风湿性关节炎的临床观察

观察报刺法联合火针治疗寒湿痹阻型类风湿性关节炎的临床疗效。【方法】 将72例寒湿痹阻型类风湿性关节炎 患者随机分为观察组和对照组,每组各 36例,对照组给予火针联合普通针刺治疗,观察组给予火针联合毫针报刺法治疗。 隔天治疗1次,每周治疗3次,治疗2周为1个疗程,持续治疗2个疗程。治疗4周后,评价2组临床疗效,观察2组患者治 疗前后疼痛视觉模拟量表（VAS）评分与焦虑自评量表（SAS）评分的变化情况,以及关节晨僵时间的情况。比较2组患者治疗 前后关节肿胀个数以及关节压痛个数的变化情况。【结果】（1）治疗后,2组患者的关节晨僵时间明显改善（P＜0.05）,且观察 组在改善关节晨僵方面明显优于对照组,差异有统计学意义（P＜0.05）。（2）治疗后,2组患者的关节肿胀个数、关节压痛个 数明显改善（P＜0.05）,且观察组在改善关节肿胀和关节压痛方面明显优于对照组,差异有统计学意义（P＜0.05）。（3）治疗 后,2组患者的 VAS及 SAS评分明显改善（P＜0.05）,且观察组在改善 VAS及 SAS评分方面明显优于对照组,差异有统计学 意义（P＜0.05）。（4）观察组的总有效率为 91.66% （33/36）,对照组为 77.78%（28/36）。观察组疗效优于对照组,差异有统计学 意义（P＜0.05）。【结论】 报刺法结合火针治疗寒湿痹阻型类风湿性关节炎,能明显改善患者的临床症状,减轻患者的焦虑情 绪,从而提高患者生活质量,疗效显著。     

建立局部性家兔肠系膜静脉血栓模型

目的探讨建立局部性家兔肠系膜静脉血栓（MVT）模型的方法。方法于2008年1月,将36只家兔随机均分为3组,肠系膜前静脉局部阻断加静脉分支注射凝血酶,A组80U/mL,B组40U/mL,C组注射生理盐水1mL。观测血栓形成时间、范围和周围静脉血D-二聚体（DD）变化。结果 A、B两组均可建立控制范围内稳定MVT模型,血栓形成时间分别为（15.6±2.0）min和（22.3±2.5）min,两组比较有统计学意义（P〈0.001）;术前A、B两组DD为（68.4±5.7）ng/mL、（72.7±6.4）ng/mL,血栓形成后为（209.0±24.0）ng/mL、（215.4±17.6）ng/mL,组内比较有统计学意义（P〈0.001）;C组不形成血栓。结论局部静脉阻断加凝血酶注射法可建立稳定和范围可控的MVT模型,建模前后血浆DD值有一定实验参考价值。     

Recognition and Management of Venous Leg Ulcers

The management of venous leg ulcers can pose quite a challenge to patients and health care providers. This condition can significantly impact quality of life and impose a huge financial burden on the patient and health care system. This review article will discuss the importance of early recognition of high-risk patients, with evaluation and initiation of appropriate multimodality treatment, which must be done in a timely and efficient manner.     

初级卫生保健人员的糖尿病继续医学教育方式

检索、分类并分析糖尿病继续医学教育方式（EMDCME）的相关文章.将EMDCME分为6类,即分发教育资料、教育性会议、舆论领袖、共识进程、审查和反馈、教育推广访问,分析上述方式优劣.根据当地初级卫生保健人员的实际情况选择EMDCME,合理评估教育效果及经济学效益,探索出最合适的教育方式,取得最佳的卫生经济学效益.     

老年人外周动脉粥样硬化与冠心病

外周动脉粥样硬化(AS)常与冠状动脉(冠脉)病变同时存在,近年研究已证实,颈动脉AS与冠心病(CHD)存在相同的病理生理变化和危险因素[1],二者有密切的关系;颈动脉AS在一定程度上代表了全身AS的进展情况。