全文获取类型
收费全文 | 47656篇 |
免费 | 4520篇 |
国内免费 | 3679篇 |
专业分类
耳鼻咽喉 | 298篇 |
儿科学 | 431篇 |
妇产科学 | 482篇 |
基础医学 | 5639篇 |
口腔科学 | 740篇 |
临床医学 | 6924篇 |
内科学 | 7480篇 |
皮肤病学 | 591篇 |
神经病学 | 2602篇 |
特种医学 | 1939篇 |
外国民族医学 | 23篇 |
外科学 | 4384篇 |
综合类 | 7692篇 |
现状与发展 | 15篇 |
一般理论 | 7篇 |
预防医学 | 2918篇 |
眼科学 | 1468篇 |
药学 | 5036篇 |
81篇 | |
中国医学 | 2836篇 |
肿瘤学 | 4269篇 |
出版年
2024年 | 152篇 |
2023年 | 855篇 |
2022年 | 2035篇 |
2021年 | 2614篇 |
2020年 | 1963篇 |
2019年 | 1647篇 |
2018年 | 1756篇 |
2017年 | 1513篇 |
2016年 | 1454篇 |
2015年 | 2164篇 |
2014年 | 2635篇 |
2013年 | 2335篇 |
2012年 | 3454篇 |
2011年 | 3881篇 |
2010年 | 2360篇 |
2009年 | 1850篇 |
2008年 | 2475篇 |
2007年 | 2563篇 |
2006年 | 2484篇 |
2005年 | 2583篇 |
2004年 | 1486篇 |
2003年 | 1329篇 |
2002年 | 1110篇 |
2001年 | 1101篇 |
2000年 | 1152篇 |
1999年 | 1210篇 |
1998年 | 788篇 |
1997年 | 802篇 |
1996年 | 619篇 |
1995年 | 572篇 |
1994年 | 516篇 |
1993年 | 305篇 |
1992年 | 381篇 |
1991年 | 310篇 |
1990年 | 285篇 |
1989年 | 245篇 |
1988年 | 235篇 |
1987年 | 211篇 |
1986年 | 159篇 |
1985年 | 111篇 |
1984年 | 52篇 |
1983年 | 39篇 |
1982年 | 21篇 |
1981年 | 13篇 |
1980年 | 13篇 |
1979年 | 12篇 |
1978年 | 2篇 |
1977年 | 1篇 |
1966年 | 1篇 |
1959年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
51.
本实验利用辣根过氧化物酶(HRP)、胶体金标记法显示小鼠腹腔巨噬细胞(Mφ)表面膜刀豆素A(conA)受体的分布及conA受体介导的吞噬活动。结果显示,conA受体在Mφ膜表面呈均匀分布。随着conA凝集素与Mφ膜表面conA受体作用时间的延长,conA受体介导的内吞活性增强。 相似文献
52.
辽宁满族和汉族指纹白线正常值分析 总被引:2,自引:0,他引:2
本文报告了辽宁满族和汉族人指纹白线的调查结果。满族和汉族人的指纹白线出现率分别是7.14%和31.73%。满族人的指纹白线出现率低于汉族、维吾尔族、德国人、波兰人和美国白人。作者注意到满族人的指纹白线不存在左右手间和性别间的差异,而汉族人性别间差异显著。十指均有白线频率为1.91%(满族)和10.5%(汉族)。十指均有白线的白线平均数满族为39.3±6.6,汉族为35.5±10.6。五指中指纹白线出现率从高到低的顺序在满族为Ⅰ、Ⅳ、Ⅲ、Ⅱ、Ⅴ,在汉族为Ⅳ、Ⅲ、Ⅱ、Ⅰ、Ⅴ。存在民族间的差异。 相似文献
53.
化疗药物配制方法探讨 总被引:10,自引:0,他引:10
为探讨化疗药物正确的配制方法,有效地预防化疗药物可能存在的职业危害,并使病人得到准确的用药剂量。提出配制化疗药物应在专门的化疗药物配制阃配制,使用特制的层流净化操作台,不具备条件者可在排风设备良好、空气流通的场所内配制。配制人员必须熟练掌握化疗药物的药理知识,配制时应严格做好查对工作。 相似文献
54.
H N Qian J Feng H Cui B S Gao S L Qin T Y Fu P Wei Z Y Fu S M Gao Z Y Yang 《中华医学杂志(英文版)》1991,104(2):109-113
Monoclonal antibody 131I-COC183B2, developed in our laboratory and proved to fit for human treatment was injected intraperitoneally or subcutaneously in 13 patients. In 8 cases with i.p. injection the disease corresponded with the image, i.e. 3 primary ovarian epithelial cancers showed positive images, 1 ovarian Krukenberg tumor was negative and the other 4 negative images included 1 uterine myoma and 3 ovarian teratomas. In the subcutaneous injection group, 4 cases had ovarian carcinoma, surgery and chemotherapy. Two negative images corresponded with the clinical status-in good health, another negative case had metastatic left supraclavicular lymph node due to ovarian mucinous adenocarcinoma. The last negative image in this group was a case of benign ovarian teratoma which was proved after surgery. The 1 positive case was waiting to be proved by a scheduled third operation. The computer scintigram calculation of T/NT was 5.35 to 13.7. The results suggest that this monoclonal antibody can be used for radioimmunoimaging for the localization of ovarian carcinoma, which is not only helpful for clinical staging and differential diagnosis but is also a good follow-up method.
相似文献
55.
A nuclear protein associated with human cancer cells binds preferentially to a human repetitive DNA sequence. 总被引:2,自引:1,他引:1
下载免费PDF全文
![点击此处可从《Proceedings of the National Academy of Sciences of the United States of America》网站下载免费的PDF全文](/ch/ext_images/free.gif)
M L Law J Z Gao T T Puck 《Proceedings of the National Academy of Sciences of the United States of America》1989,86(21):8472-8476
A protein (Rp66) of 66 kDa was shown by DNA-binding protein blot assay to bind to a human repetitive DNA sequence (low-repeat sequence; LRS) in each of 10 transformed human cell lines examined. This protein-DNA interaction was not observed in 11 normal human cell cultures or in the Chinese hamster cell line CHO-K1. Gel retardation assay confirmed the specificity of the protein-DNA binding between Rp66 and LRS. In a histiocytic lymphoma human cell line, U937, that can be induced to differentiate in the presence of phorbol ester, this binding disappeared after cell differentiation. These together with other results cited suggest a regulatory role for these repetitive sequences in the human genome, with particular application to cancer. 相似文献
56.
Expression of Anion Exchanger 1 Sequestrates p16 in the Cytoplasm in Gastric and Colonic Adenocarcinoma
下载免费PDF全文
![点击此处可从《Neoplasia (New York, N.Y.)》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Wei-Wei Shen Jun Wu Li Cai Bing-Ya Liu Yan Gao Guo-Qiang Chen Guo-Hui Fu 《Neoplasia (New York, N.Y.)》2007,9(10):812-819
p16INK4A (p16) binds to cyclin-dependent kinase 4/6 and negatively regulates cell growth. Recent studies have led to an understanding of additional biologic functions for p16; however, the detailed mechanisms involved are still elusive. In this article, we show an unexpected expression of anion exchanger 1 (AE1) in the cytoplasm in poorly and moderately differentiated gastric and colonic adenocarcinoma cells and in its interaction with p16, thereby sequestrating the protein in the cytoplasm. Genetic alterations of p16 and AE1 were not detectable. Forced expression of AE1 in these cells sequestrated more p16 in the cytoplasm, whereas small interfering RNA-mediated silencing of AE1 in the cells induced the release of p16 from the cytoplasm to the nucleus, leading to cell death and growth inhibition of tumor cells. By analyzing tissue samples obtained from patients with gastric and colonic cancers, we found that 83.33% of gastric cancers and 56.52% of colonic cancers coexpressed AE1 and p16 in the cytoplasm. We conclude that AE1 plays a crucial role in the pathogenesis of gastric and colonic adenocarcinoma and that p16 dysfunction is a novel pathway of carcinogenesis. 相似文献
57.
58.
和谐是发展的条件,和谐产生动力。如何利用和谐因素消除不和谐因素,是中医发展值得重视和研究的问题。对中医发展中的和谐方面进行了探讨。 相似文献
59.
利福喷汀治疗耐利福平肺结核的疗效分析 总被引:1,自引:0,他引:1
目的分析增加利福喷汀的用药剂量对利福平耐药肺结核的疗效,以评价利福喷汀在治疗利福平耐药患者中的作用。方法根据药敏结果选取耐利福平肺结核患者101例,分为治疗1组(利福平低耐),治疗2组(利福平高耐),两组治疗方案中均含利福喷汀,对照组(利福平高耐)治疗方案中不合利福喷汀。观察治疗后3、18个月疗效。结果治疗1组在各阶段的痰菌阴转率、病灶吸收和空洞闭和情况明显优于治疗2组和对照组,两组差异有统计学意义;治疗2组的痰菌阴转率、病灶吸收和空洞闭和情况略优于对照组,两者差异无统计学意义。结论对利福平耐药的患者尤其在利福平低度耐药病例中,可以选择增加利福喷汀用药剂量的方案进行治疗,在利福平高度耐药病例中利福喷汀的作用不能确定。 相似文献
60.
Gerard J Molloy Derek W Johnston Chuan Gao Miles D Witham Joan M Gray Ishbel S Argo Allan D Struthers Marion E T McMurdo 《European journal of cardiovascular prevention and rehabilitation》2006,13(3):381-387
BACKGROUND: The impact of exercise programmes for heart failure on those close to the patient is largely unknown. We examined the effect of a hospital and home-based exercise intervention on burden, anxiety and depression of informal caregivers. DESIGN: The study was a randomized, controlled trial. Heart failure patients were randomized to a seated 12-week hospital-based exercise programme. Caregiver measures were gathered at baseline, 3 months later and 6 months following baseline. METHODS: Sixty caregivers (mean age 63.4 years, 65% female) of heart failure patients (n = 82, mean age 80.5 years, 44% female) participating in a trial of an exercise intervention were recruited. Caregiver burden, anxiety and depression were assessed. RESULTS: There were no differences in caregiver burden, depression or anxiety between the two groups of caregivers at baseline (caregiver burden, patient control 33.1 versus patient exercise 34.1; anxiety 4.1 versus 5.5; depression 2.8 versus 3.8). At 3 months there were no differences between caregivers in the two groups on outcomes. At 6-month follow-up caregivers of heart failure patients in the exercise group had burden scores that were significantly worse than the control group. There were no differences between the carers of exercise and control groups in anxiety and depression. Levels of anxiety and depression in the entire carer sample were marginally higher than reference values in a healthy non-clinical sample. CONCLUSION: The present exercise interventions for frail older patients did not benefit caregivers and was associated with an increase in caregiver burden. We suggest that future exercise interventions for heart failure patients should actively incorporate informal caregivers into research designs. 相似文献