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101.
Role of nitric oxide in subventricular zone neurogenesis 总被引:3,自引:0,他引:3
Esperanza R. Matarredona Maribel Murillo-Carretero Bernardo Moreno-Lpez Carmen Estrada 《Brain Research Reviews》2005,49(2):355
A possible role of nitric oxide (NO) in adult neurogenesis has been suggested based on anatomical findings showing that subventricular zone (SVZ) neuroblasts are located close to NO-producing cells, and on the known antiproliferative actions of NO in many cell types.Experiments have been performed in rodents with systemic and intracerebroventricular administrations of the NO synthase (NOS) inhibitor L-NAME. NOS inhibition leads to significant increases in the number of proliferating cells in the SVZ and olfactory bulb (OB). NO exerts its cytostatic action preferentially on the cell population expressing nestin but not βIII-tubulin, which may correspond to the type C cells described in the SVZ. The negative effect of NO on SVZ cell proliferation has also been confirmed in SVZ primary cultures.An inhibition of the tyrosine kinase activity of the epidermal growth factor receptor (EGFR) is described as one of the molecular mechanisms responsible for the antiproliferative effect of NO in SVZ cells. Biochemical data supporting this conclusion has been obtained using the neuroblastoma cell line NB69, which endogenously expresses the EGFR. In these cells, the antimitotic action of NO occurs upon inhibition of the EGFR tyrosine phosphorylation, probably by a direct S-nitrosylation of the receptor.The latest published reports on NO and neurogenesis indicate that NO physiologically participates in the control of adult neurogenesis by modulating the proliferation and fate of the SVZ progenitor cells. These effects might be partially due to a direct inhibition of the EGFR by S-nitrosylation. 相似文献
102.
Andoh T.; Blanck TJJ.; Nikonorov I.; Recio-Pinto E. 《British journal of anaesthesia》1997,78(1):66-74
Volatile anaesthetics are known to increase leakage of calcium from the
light fraction of skeletal sarcoplasmic reticulum (L-SR) which has no
calcium release channels. To explore the role of the lipid environment, we
have examined the effect of volatile anaesthetics on calcium conductance
(gCa) of lipid membranes. Planar lipid bilayers were formed with a mixture
of synthetic phospholipids and cholesterol, resembling the composition of
SR membranes, or with lipids extracted from skeletal L-SR, gCa was
estimated by calculating the calcium transference number (tCa) using
diffusion potential measurements. Membranes formed with L- SR-extracted
lipids had a higher gCa than membranes formed with synthetic lipids.
Volatile anaesthetics increased total conductance and gCa in a
dose-dependent manner, but did not affect tCa or membrane specific
capacitance. In membranes formed with L-SR-extracted lipids, isoflurane
induced the largest increase in gCa (1260 (SEM 304) % increase, n = 4, 0.94
mmol litre-1), followed by enflurane (264 (75)%, n = 5, 1.88 mmol litre-1)
and halothane (53 (33)%, n = 5; 1.54 mmol litre-1). In membranes formed
with synthetic lipids, volatile anaesthetic-induced increases in gCa
followed the same trend but were larger. Volatile anaesthetics increased
gCa without changing the ionic selectivity of membranes. However, the
magnitude of the increase in gCa in the presence of volatile anaesthetics
cannot account for the previously observed calcium leakage from L-SR
vesicles. Therefore, the volatile anaesthetic-induced increase in calcium
leakage in L-SR vesicles must be mediated via other pathways involving
membrane proteins.
相似文献
103.
Susana Vives MD David Martínez-Cuadrón MD Juan Bergua Burgues MD Lorenzo Algarra MD Mar Tormo MD María Pilar Martínez-Sánchez MD Josefina Serrano MD Pilar Herrera MD Fernando Ramos MD Olga Salamero MD Esperanza Lavilla MD José L. López-Lorenzo MD Cristina Gil MD Belén Vidriales MD Jose F. Falantes MD Alfons Serrano MD Jorge Labrador MD María J. Sayas MD María Á. Foncillas MD María L. Amador Barciela MD María Teresa Olave MD Mercedes Colorado MD Adriana Gascón MD María Á. Fernández MD Adriana Simiele MD Manuel M. Pérez-Encinas MD Rebeca Rodríguez-Veiga MD Olga García MS Joaquín Martínez-López MD Eva Barragán PhD Bruno Paiva PhD Miguel Á. Sanz MD Pau Montesinos MD for the PETHEMA Group 《Cancer》2021,127(12):2003-2014
104.
E. Martha Pérez Armendariz Monica Norcini Beatriz Hernández-Tellez Andrés Castell-Rodríguez Cristina Coronel-Cruz Raquel Guerrero Alquicira Alexandra Sideris Esperanza Recio-Pinto 《Acta histochemica》2018,120(3):168-178
Previous studies have shown that following peripheral nerve injury there was a downregulation of the gap junction protein connexin 36 (Cx36) in the spinal cord; however, it is not known whether Cx36 protein is expressed in the dorsal root ganglia (DRGs), nor if its levels are altered following peripheral nerve injuries. Here we address these aspects in the adult rat lumbar DRG. Cx36 mRNA was detected using qRT-PCR, and Cx36 protein was identified in DRG sections using immunohistochemistry (IHC) and immunofluorescence (IF). Double staining revealed that Cx36 co-localizes with both anti-β-III tubulin, a neuronal marker, and anti-glutamine synthetase, a satellite glial cell (SGC) marker. In neurons, Cx36 staining was mostly uniform in somata and fibers of all sizes and its intensity increased at the cell membranes. This labeling pattern was in contrast with Cx36 IF dots mainly found at junctional membranes in islet beta cells used as a control tissue. Co-staining with anti-Cx43 and anti-Cx36 showed that whereas mostly uniform staining of Cx36 was found throughout neurons and SGCs, Cx43 IF puncta were localized to SGCs. Cx36 mRNA was expressed in normal lumbar DRG, and it was significantly down-regulated in L4 DRG of rats that underwent sciatic nerve injury resulting in persistent hypersensitivity. Collectively, these findings demonstrated that neurons and SGCs express Cx36 protein in normal DRG, and suggested that perturbation of Cx36 levels may contribute to chronic neuropathic pain resulting from a peripheral nerve injury. 相似文献
105.
Martínez-Sernández Victoria Perteguer María J. Hernández-González Ana Mezo Mercedes González-Warleta Marta Orbegozo-Medina Ricardo A. Romarís Fernanda Paniagua Esperanza Gárate Teresa Ubeira Florencio M. 《Parasitology research》2018,117(5):1521-1534
Parasitology Research - Infections caused by Fasciola hepatica are of great importance in the veterinary field, as they cause important economic losses to livestock producers. Serodiagnostic... 相似文献
106.
The association of low birth weight with serum C reactive protein in 3‐year‐old children living in Cuba: A population‐based prospective study
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107.
Pachkoria K Lucena MI Crespo E Ruiz-Cabello F Lopez-Ortega S Fernandez MA Romero-Gomez M Madrazo A Durán JA de Dios AM Borraz Y Navarro JM Andrade RJ;Spanish Group for the Study of Drug-Induced Liver Disease 《Journal of hepatology》2008,49(1):107-114
BACKGROUND/AIMS: The aim of this study was to assess whether genetic polymorphism of three important candidate cytokine genes, IL-10 (-1082G/A, -819C/T, and -592C/A), IL-4 (-590C/T) and TNF-alpha (-308G/A), play a role in the susceptibility to developing drug-induced liver injury (DILI), and in determining its phenotypic expression and severity. METHODS: Cytokine genotyping was analysed using TaqMan 5' allelic discrimination assay in 140 DILI patients (mean age 51 y, range 13-82, with equal sex distribution) included in the Spanish Registry and 268 healthy controls. RESULTS: Genotypes, haplotypes and allele frequencies were similar for both cases and controls. The low IL-10 producing haplotype was more prevalent in DILI patients with the absence of peripheral blood eosinophilia (Pc=0.004, OR=5.29, 95% CI: 2.04-13.67), revealing significantly lower median eosinophil counts (0.19 x 10(9)L; P<0.0002) compared to the intermediate (0.24 x 10(9)L) and high (0.40 x 10(9)L) IL-10 haplotypes. All cases with serious DILI outcome carried low or intermediate IL-10 producing haplotype and had normal or low eosinophil counts. CONCLUSIONS: IL-10, IL-4 and TNF-alpha genetic polymorphisms were not related to the risk of developing DILI. Low IL-10 producing haplotype is associated with low eosinophil count, absence of eosinophilia and may be associated with worse clinical outcome from DILI. 相似文献
108.
GE Furtado Mda L Sousa Tde S Barbosa RS Wada Ede L Martínez-Mier ME Almeida 《Cadernos de saúde pública / Ministério da Saúde, Funda??o Oswaldo Cruz, Escola Nacional de Saúde Pública》2012,28(8):1493-1505
This study aimed to validate the Child's and Parent's Questionnaire about Teeth Appearance and to evaluate concerns relative to fluorosis among 213 pairs of parents and 12-year-old children from two regions of Brazil. Reliability was assessed by Cronbach's alpha and intraclass correlation coefficient, and construct and criterion validity by Spearman's correlations. Student t-test was used to compare the two regions and to assess parent/child agreement. Internal consistency was acceptable, and test-retest reliability was moderate to excellent. Perception of moderate to severe fluorosis and clinical data were significantly correlated, as were perception of fluorosis and subjects' concerns. Although parents from Rafael Arruda, Ceará State, showed a higher perception of fluorosis, parental concern was greater in Piracicaba, S?o Paulo State. Parents were more worried and dissatisfied with their children's dental appearance than the children themselves. This version of the questionnaire proved to be valid and reliable for assessing children's and parents' perceptions of dental fluorosis. 相似文献
109.
110.
Milagros Rico-Blázquez Esperanza Escortell-Mayor Isabel del-Cura-González Teresa Sanz-Cuesta Pilar Gallego-Berciano Gonzalo de las Casas-Cámara Sonia Soto-Díaz Petra García-Sanz Natalie Harris-de-la-Vega María Martín-Martín Lorena Domínguez-Pérez Araceli Rivera-Álvarez Juan Antonio Sarrión-Bravo Concepción Pérez-de-Hita Marisol de-Frías-Redondo Carmen Ferrer-Arnedo Montserrat Hernández-Pascual Antonio Valdivia-Pérez Yolanda Ramallo Fariña 《BMC nursing》2014,13(1):1-10