血管生成抑制蛋白1和结肠癌转移相关蛋白-1在肺鳞状细胞癌中的表达及其临床意义

目的 检测肺鳞状细胞癌(LSCC)中血管生成抑制蛋白1(vasohibin-1)和结肠癌转移相关蛋白-1(MACC1)蛋白的表达及其相互之间的关系,并探讨它们与LSCC患者临床病理因素的关系.方法 利用免疫组织化学ElivisionTM plus方法检测160例LSCC和80例正常肺组织中vasohibin-1和MACC1蛋白的表达情况.结果 在LSCC组织中,vasohibin-1和MACC1蛋白的表达阳性率分别为59.4%和57.5%;对照组中,vasohibin-1和MACC1蛋白的表达阳性率分别为11.3%和8.8%,其表达差异在两组之间有统计学意义(P<0.05).vasohibin-1和MACC1蛋白的表达阳性率在LSCC患者肿瘤组织的不同分化程度之间、淋巴结转移与否之间以及TNM不同分期之间差异均有统计学意义(P<0.05);且vasohibin-1蛋白的表达阳性率与MACC1蛋白的表达阳性率之间呈正相关关系(P<0.001).Kaplan-Meier生存分析表明在LSCC中vasohibin-1和MACC1蛋白阳性表达组患者术后总的生存时间明显低于其阴性组患者,log-rank分析其差异均有统计学意义.多因素回归分析表明vasohibin-1和MACC1蛋白的阳性表达及TNM分期是影响LSCC患者术后生存的独立预后因子.结论 异常表达的vasohibin-1和MACC1可能参与了LSCC的发展,并促进其浸润和转移;在LSCC患者中联合检测vasohibin-1和MACC1蛋白的表达对预测其发展和预后均有重要意义.     

梅毒血清固定患者血清IL-10和IL-12水平及相关性分析

目的:通过检测正常人群、梅毒未治疗患者、梅毒治疗后非梅毒螺旋体抗原血清试验转阴患者和梅毒血清固定患者血清IL-10、IL-12水平,并对梅毒血清固定患者血清IL-10和IL-12作相关性分析。以探讨细胞免疫与梅毒血清固定的关系,为有效治疗梅毒血清固定患者提供一定的理论依据。方法:采用双抗体夹心ELISA法分别检测正常人群组、梅毒未治疗组、梅毒治疗转阴组和梅毒血清固定组的IL-10和IL-12水平,将各组IL-10和IL-12水平行统计学分析。对梅毒血清固定组中IL-10与IL-12作相关性分析。结果:梅毒血清固定组患者IL-10水平显著高于正常对照组(P<0.01)、治疗后阴转组(P<0.01)及梅毒未治疗组(P<0.05);梅毒血清固定组患者IL-12水平与正常对照组、治疗后阴转组及梅毒未治疗组之间差异无显著性(P>0.05);梅毒血清固定组患者IL-10与IL-12水平之间无明显相关性(r=0.417,P>0.05)。结论:梅毒血清固定组患者机体内细胞免疫受到一定程度的抑制,发生了免疫不平衡,这种免疫抑制和不平衡可能造成了梅毒螺旋体能够逃脱机体的免疫监视而残存,成为引起血清固定的原因之一。     

经皮椎体后凸成形术治疗骨质疏松性胸腰椎压缩性骨折的临床观察

目的探讨经皮椎体后凸成形术治疗骨质疏松性胸腰椎压缩性骨折的疗效。方法我院采用经皮椎体后凸成形术治疗骨质疏松性胸腰椎压缩性骨折69例。结果术后随访不同时间其VAS评分较术前明显改善。术后疗效评价:治愈18例,显效30例,有效19例,无效2例。结论采用采用经皮椎体后凸成形术治疗骨质疏松性胸腰椎压缩性骨折的临床疗效确切,值得临床推广应用。     

抗程序化细胞死亡基因5抗体与类风湿关节炎相关性研究

目的 探讨抗程序化细胞死亡基因5（PDCD5）抗体在类风湿关节炎（RA）中的意义.方法 采用酶联免疫吸附法（ELISA）对55例RA 患者和44例健康对照者（NC）外周血清中抗PDCD5抗体的水平进行检测,并与RA患者相关临床资料、实验室指标及影像学资料进行统计学分析.结果 55例RA患者和44例NC外周血清中抗PDCD5抗体的水平分别为（18.06±4.70）μg/L,（13.62±4.35）μg/L,阳性率分别为18.2%（10/55）,4.5%（2/44）,二者在抗体水平和阳性率上差异有统计学意义（P＜0.05）.抗PDCD5抗体阳性组Sharp评分,类风湿因子（RF）和抗环瓜氨酸肽（CCP）抗体水平明显高于抗PDCD5抗体阴性组,抗PDCD5抗体与抗CCP抗体呈正相关（r=0.374,P=0.01,2-tailed）.结论 抗PDCD5抗体与RA密切相关,且抗体阳性患者易出现骨侵蚀,有望成为诊断早期RA的实验室指标之一.     

407例梅毒调查分析

目的:探讨近期本区域梅毒的流行病学及血清学特征。方法:回顾性分析2009年我科全年确诊的梅毒患者的性别、年龄、职业、文化程度、传播情况及血清学特点。结果:梅毒患者男女比例为1∶1.41,发病年龄主要集中在21~40岁,女性患者年龄明显小于男性,差异有显著统计学意义(P<0.05),绝大部分患者为已婚有性伴(332/407),文化程度主要以初中为主,高学历患者少,职业中无业、工人、农民占前3位,仅75人承认近3月内有非婚性伴,其中46人从未使用安全套,每次使用安全套患者仅有10人,3人近3月内使用过毒品。男性梅毒患者血清TRUST滴度以高滴度为主,女性患者低滴度相对较高,差异有显著统计学意义(P<0.05)。结论:本地区梅毒以女性、初中文化、无业、工人和农民居多,梅毒的防治也应将这些人群作为重点防治对象。     

Differences in T cell subsets between men and women with idiopathic thrombocytopenic purpura

Idiopathic thrombocytopenic purpura (ITP) is an autoimmune disorder, occurring predominantly in women. We studied by flow cytofluorimetry the T cell subsets in men and women with ITP and compared them with healthy sex-matched volunteers. In healthy controls, women were found to have higher proportions of T helper/inducer (Th/i) and lower T suppressor/cytotoxic (Ts/c) lymphocytes and consequently higher Th/i:Ts/c ratios than men. Accordingly, in clinical surveys, patients and controls should be matched for sex for proper comparisons. In patients with ITP in its active phase, an imbalance in T cell subsets was found in both sexes. The perturbation was more severe in women who had a marked decrease in number and proportion of Th/i lymphocytes and an increase in the proportion of Ts/c lymphocytes, whereas in men only, the proportion of Th/i lymphocytes was decreased. When patients with active disease were compared to those with ITP in remission, the decrease in Th/i subsets still persisted in both sexes but the Ts/c subset in women had returned to normal proportions. Therefore, the immune imbalance in ITP is more marked in women than men; imbalances in both Th/i and Ts/c are present in women while Ts/c appears not to be involved in men.     

多发性大动脉炎合并心肌梗死5例临床分析

目的 探讨多发性大动脉炎合并心肌梗死患者的临床特点、冠状动脉病变情况、治疗方法及转归.方法 回顾性分析2002年1月至2006年4月我院收治的多发性大动脉炎合并心肌梗死患者的症状、体征、实验室检查结果、冠状动脉造影结果、治疗方法及转归资料.结果 本组多发性大动脉炎Ⅰ型2例,Ⅱ型1例,Ⅲ型2例.其中,合并冠状动脉三支病变3例,双支病变1例,单支病变1例.冠状动脉病变多见于左右冠状动脉近段,中段及分支也可受累.3例患者行经皮冠脉成形术(PTCA)加支架植入术后病情好转,1例经药物治疗后仍有胸痛发作,1例于住院期间死亡.结论 多发性大动脉炎合并心肌梗死并不少见,受累的冠状动脉病变较重,病死率较高.     

新生大鼠注射尘螨对其发育后期气道炎症指标作用的研究

目的研究早期接触屋尘螨(HDM)能否加重后期哮喘发作时的气道炎症及其相关免疫学机理.方法 HDM组新生Wistar大鼠生后24h内立即皮下注射HDM 0.1ml,Asthma组和Control组则注射N.S,于日龄第30天起用OVA或N.S制作哮喘模型,最后进行支气管肺泡灌洗及灌洗液(BALF)中嗜酸性粒细胞计数,检测血清OVA特异性IgE抗体水平(A值).结果 HDM组EOS百分比、血清OVA特异性IgE抗体A值分别为(37.07±1.10)%、0.204±0.023,在3个组中为最高.结论生后早期接触HDM能加重以后气道变态反应性炎症,其原因与TH1/TH2失衡有关.     

Correlations between mean LH levels and LH pulse characteristics: differences between normal and anovulatory women

OBJECTIVE: Since LH secretion occurs as a series of pulses, relationships between mean LH levels and LH pulse characteristics are to be expected. The aim of this study was to determine whether such relationships are similar in normal women and anovulatory patients. DESIGN: We studied the correlations between mean LH levels and the products amplitude x frequency and area x frequency of LH pulses in normal women and in patients with disorders of ovulation. Blood samples were taken from each subject every 10 minutes during 6 hours on the 8th day after the last menses. PATIENTS: The patients were divided into three groups: patients with polycystic ovary syndrome (n = 11), patients with idiopathic anovulation (n = 14) and patients with short luteal phase (n = 13). Their results were compared to those of 12 normal women. MEASUREMENTS: LH was evaluated with an immunoradiometric assay. LH data were analysed with a 3 standard deviation threshold criterion for significant peaks, and with cluster analysis algorithm using 1, 2.5 and 5% false positive error rates and 'optimal parameters' (which give less than 5% false positive and false negative error rates in LH male data). RESULTS: Highly significant correlations between amplitude x frequency, area x frequency and mean LH were found in normals and patients with short luteal phase; no significant correlation was found in patients with polycystic ovary syndrome, while significant correlations were found in patients with idiopathic anovulation only with some of our criteria for peak detection. CONCLUSION: The differences that we found between the groups suggest that when commonly used methods are employed to determine LH pulse characteristics, most of the significant LH pulses are taken into account in normals and patients with short luteal phase, but not in anovulatory patients, especially in patients with polycystic ovary syndrome. This method using two correlations appears to be a simple and useful way to show the differences in the mechanisms by which mean LH levels are achieved in normal subjects and patients.