全文获取类型
收费全文 | 6182篇 |
免费 | 328篇 |
国内免费 | 47篇 |
专业分类
耳鼻咽喉 | 40篇 |
儿科学 | 237篇 |
妇产科学 | 136篇 |
基础医学 | 1338篇 |
口腔科学 | 113篇 |
临床医学 | 588篇 |
内科学 | 1103篇 |
皮肤病学 | 114篇 |
神经病学 | 582篇 |
特种医学 | 330篇 |
外科学 | 689篇 |
综合类 | 25篇 |
一般理论 | 1篇 |
预防医学 | 360篇 |
眼科学 | 87篇 |
药学 | 466篇 |
中国医学 | 4篇 |
肿瘤学 | 344篇 |
出版年
2022年 | 46篇 |
2021年 | 89篇 |
2020年 | 65篇 |
2019年 | 80篇 |
2018年 | 99篇 |
2017年 | 80篇 |
2016年 | 86篇 |
2015年 | 125篇 |
2014年 | 137篇 |
2013年 | 167篇 |
2012年 | 274篇 |
2011年 | 269篇 |
2010年 | 160篇 |
2009年 | 183篇 |
2008年 | 285篇 |
2007年 | 309篇 |
2006年 | 325篇 |
2005年 | 309篇 |
2004年 | 296篇 |
2003年 | 301篇 |
2002年 | 297篇 |
2001年 | 177篇 |
2000年 | 168篇 |
1999年 | 164篇 |
1998年 | 71篇 |
1997年 | 63篇 |
1996年 | 45篇 |
1995年 | 54篇 |
1994年 | 48篇 |
1993年 | 35篇 |
1992年 | 78篇 |
1991年 | 62篇 |
1990年 | 71篇 |
1989年 | 77篇 |
1988年 | 61篇 |
1987年 | 70篇 |
1986年 | 59篇 |
1985年 | 63篇 |
1984年 | 48篇 |
1981年 | 32篇 |
1979年 | 38篇 |
1935年 | 32篇 |
1934年 | 36篇 |
1933年 | 35篇 |
1928年 | 33篇 |
1926年 | 33篇 |
1925年 | 35篇 |
1924年 | 36篇 |
1923年 | 39篇 |
1922年 | 50篇 |
排序方式: 共有6557条查询结果,搜索用时 15 毫秒
31.
32.
Gillingham MB Connor WE Matern D Rinaldo P Burlingame T Meeuws K Harding CO 《Molecular genetics and metabolism》2003,79(2):114-123
Current dietary therapy for long-chain 3-hydroxyacyl-CoA dehydrogenase (LCHAD) or trifunctional protein (TFP) deficiency consists of fasting avoidance, and limiting long-chain fatty acid (LCFA) intake. This study reports the relationship of dietary intake and metabolic control as measured by plasma acylcarnitine and organic acid profiles in 10 children with LCHAD or TFP deficiency followed for 1 year. Subjects consumed an average of 11% of caloric intake as dietary LCFA, 11% as MCT, 12% as protein, and 66% as carbohydrate. Plasma levels of hydroxypalmitoleic acid, hydroxyoleic, and hydroxylinoleic carnitine esters positively correlated with total LCFA intake and negatively correlated with MCT intake suggesting that as dietary intake of LCFA decreases and MCT intake increases, there is a corresponding decrease in plasma hydroxyacylcarnitines. There was no correlation between plasma acylcarnitines and level of carnitine supplementation. Dietary intake of fat-soluble vitamins E and K was deficient. Dietary intake and plasma levels of essential fatty acids, linoleic and linolenic acid, were deficient. On this dietary regimen, the majority of subjects were healthy with no episodes of metabolic decompensation. Our data suggest that an LCHAD or TFP-deficient patient should adhere to a diet providing age-appropriate protein and limited LCFA intake (10% of total energy) while providing 10-20% of energy as MCT and a daily multi-vitamin and mineral (MVM) supplement that includes all of the fat-soluble vitamins. The diet should be supplemented with vegetable oils as part of the 10% total LCFA intake to provide essential fatty acids. 相似文献
33.
Sangals J Heuer H Manzey D Lorenz B 《Experimental brain research. Experimentelle Hirnforschung. Expérimentation cérébrale》1999,129(3):378-390
A series of step-tracking experiments was conducted before, during, and after a 3-week space mission to assess the effects of prolonged microgravity on a non-postural motor-control task. In- and post-flight accuracy was affected only marginally. However, kinematic analyses revealed a considerable change in the underlying movement dynamics: too-small force and, thus, too-low velocity in the first part of the movements was mainly compensated by lengthening the deceleration phase of the primary movement, so that accuracy was regained at its end. The observed in-flight decrements in peak velocity and peak acceleration point to an underestimation of mass, in agreement with the re-interpretation hypothesis of Bock et. al. Post-flight no reversals of the in-flight changes (negative aftereffects) were found. Instead, there was a general slowing down, which could be due to post-flight physical exhaustion. 相似文献
34.
Ingo Bothe Mohi U Ahmed Farrah L Winterbottom Gudrun von Scheven Susanne Dietrich 《Developmental dynamics》2007,236(9):2397-2409
Somitic and head mesoderm contribute to cartilage and bone and deliver the entire skeletal musculature. Studies on avian somite patterning and cell differentiation led to the view that these processes depend solely on cues from surrounding tissues. However, evidence is accumulating that some developmental decisions depend on information within the somitic tissue itself. Moreover, recent studies established that head and somitic mesoderm, though delivering the same tissue types, are set up to follow their own, distinct developmental programmes. With a particular focus on the chicken embryo, we review the current understanding of how extrinsic signalling, operating in a framework of intrinsically regulated constraints, controls paraxial mesoderm patterning and cell differentiation. 相似文献
35.
Protection against murine listeriosis by an attenuated recombinant Salmonella typhimurium vaccine strain that secretes the naturally somatic antigen superoxide dismutase. 总被引:3,自引:0,他引:3
下载免费PDF全文
![点击此处可从《Infection and immunity》网站下载免费的PDF全文](/ch/ext_images/free.gif)
J Hess G Dietrich I Gentschev D Miko W Goebel S H Kaufmann 《Infection and immunity》1997,65(4):1286-1292
A recombinant (r)-Salmonella typhimurium aroA vaccine strain was constructed which secretes the naturally somatic protein of Listeria monocytogenes, superoxide dismutase (SOD), by the HlyB/HlyD/TolC export machinery. Vaccine efficacy of the SOD-bearing carrier strain was compared with that of the p60-secreting construct, S. typhimurium p60s (J. Hess, I. Gentschev, D. Miko, M. Welzel, C. Ladel, W. Goebel, and S. H. E. Kaufmann, Proc. Natl. Acad. Sci. USA 93:1458-1463, 1996). Vaccination of mice with both constructs induced protection against a lethal challenge with the intracellular pathogen, L. monocytogenes. While the somatic listerial antigen, SOD, is immunologically uncharacterized, the naturally secreted protein of L. monocytogenes, p60, is known to be highly immunogenic. Our data emphasize the high vaccine potential of r-Salmonella constructs secreting antigens of somatic or secreted origin. Moreover, they suggest that the HlyB/HlyD/TolC-based antigen delivery system with attenuated Salmonella spp. as the carrier is capable of potentiating the immune response against foreign proteins independent from their immunogenicity in and display by the natural host. 相似文献
36.
Helicobacter pylori-Specific Immune Responses of Children: Implications for Future Vaccination Strategy
下载免费PDF全文
![点击此处可从《Clinical and Vaccine Immunology : CVI》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Günter Bode Isolde Piechotowski Dietrich Rothenbacher Hermann Brenner 《Clinical and Vaccine Immunology : CVI》2002,9(5):1126-1128
We analyzed the specific anti-Helicobacter pylori immunoglobulin G (IgG) antibody profile for a sample of 824 asymptomatic schoolchildren in southern Germany (mean age, 10.7 ± 0.65 years) with an H. pylori-specific IgG enzyme-linked immunosorbent assay and Western blot analysis. The prevalence of infection was 19.8% (95% confidence interval, 17.1 to 22.7%). The immunoresponses were characterized predominantly by antibodies against low-molecular-mass antigens of 14 and 29 kDa, with a significant difference between children of German and Turkish nationalities (P = 0.0012 and P < 0.0001, respectively). 相似文献
37.
Identification of two different mutations in the PDS gene in an inbred family with Pendred syndrome 总被引:11,自引:0,他引:11
下载免费PDF全文
![点击此处可从《Journal of medical genetics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Coucke PJ Van Hauwe P Everett LA Demirhan O Kabakkaya Y Dietrich NL Smith RJ Coyle E Reardon W Trembath R Willems PJ Green ED Van Camp G 《Journal of medical genetics》1999,36(6):475-477
Recently the gene responsible for Pendred syndrome (PDS) was isolated and several mutations in the PDS gene have been identified in Pendred patients. Here we report the occurrence of two different PDS mutations in an extended inbred Turkish family. The majority of patients in this family are homozygous for a splice site mutation (1143-2A-->G) affecting the 3' splice site consensus sequence of intron 7. However, two affected sibs with non-consanguineous parents are compound heterozygotes for the splice site mutation and a missense mutation (1558T-->G), substituting an evolutionarily conserved amino acid. The latter mutation has been found previously in two Pendred families originating from The Netherlands, indicating that the 1558T-->G mutation may be a common mutation. 相似文献
38.
Obwaller A Duchêne M Bruhn H Steipe B Tripp C Kraft D Wiedermann G Auer H Aspöck H 《Parasitology research》2001,87(5):383-389
Myosins from nematode parasites elicit strong humoral and cellular immune responses and have been investigated as vaccine candidates. In this study we cloned and sequenced a cDNA coding for myosin heavy chain from Toxocara canis, a nematode parasite of canids which may also infect humans and cause various unspecific symptoms. To determine the major antigenic regions the myosin heavy chain was systematically dissected into ten overlapping recombinant fusion polypeptides which were purified by metal chelate chromatography. Single fragments were then tested for their IgG reactivity in sera from toxocarosis patients and healthy probands. Two regions, one region at the mid to carboxy-terminal end of the head domain and one region in the rod domain, were identified as major antigens, which in combination were positive with 86% of the sera. The other domains were less reactive. This shows that the patients' IgG reactivity was not directed evenly against all parts of the molecule, but was rather clustered in few regions. 相似文献
39.
Precipitable immune complexes in healthy homosexual men, acquired immune deficiency syndrome and the related lymphadenopathy syndrome. 总被引:6,自引:0,他引:6
下载免费PDF全文
![点击此处可从《Clinical and experimental immunology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Increased levels of 3% PEG precipitable circulating immune complexes (CIC) were found in healthy homosexual men, in homosexual patients with the acquired immune deficiency syndrome (AIDS), and in the AIDS related lymphadenopathy syndrome (LAS). The degree of CIC elevation increases from healthy homosexual men to LAS and AIDS. Patients suffering from AIDS associated with opportunistic infections had a more pronounced increase in CIC than patients with AIDS associated Kaposi's sarcoma. In LAS and AIDS the amount of CIC correlated with the degree of inversion of the T4/T8 lymphocyte ratio, whereas in healthy homosexual men with increased levels of CIC the T4/T8 ratio was not significantly altered. Laser nephelometric partial components analysis revealed that these complexes were of a complement poor subtype with low component levels of C4, C1q and C3c. IgM and IgG were found to be the major components. It is suggested that these CIC might represent a marker of the total antigenic burden of the immune system. Possibly, they are of prognostic and monitoring value for clinical handling of patients at risk for AIDS. 相似文献
40.
Identification and characterization of a new variant of Shiga toxin 1 in Escherichia coli ONT:H19 of bovine origin
下载免费PDF全文
![点击此处可从《Journal of clinical microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Bürk C Dietrich R Açar G Moravek M Bülte M Märtlbauer E 《Journal of clinical microbiology》2003,41(5):2106-2112
A new variant of Shiga toxin 1 (Stx1), designated Stx1d, which deviates considerably more than any other known variant from Stx1 encoded by phage 933J, was identified in an Escherichia coli strain, ONT:H19, isolated from bovine feces. The complete stx(1) gene of this strain was amplified and sequenced. Nucleotide sequence homology with stx(1) from phage 933J was only 91%, resulting in the substitution of 20 amino acids in the A subunit and 7 amino acids in the B subunit of the protein. Cell culture supernatant of this strain, which was negative for stx(2) by PCR testing, was cytotoxic to Vero cells and gave positive results in two commercial enzyme-linked immunosorbent assays for Stx. PCR primers were constructed for the specific detection of the new variant. The findings of this study suggest that Stx1 is not as conserved as thought before and that there might be more variants which cannot be detected by commonly used PCR methods. 相似文献