氯地滴眼液的含量测定

目的：采用ＨＰＬＣ法测定氯地滴眼液中氯霉素和地塞米松磷酸钠的含量。方法：色谱分析条件：ＯＤＳ柱作分析柱,流动相为甲醇／水体系,０￣８ｍｉｎ使用４０％甲醇,８￣１６ｍｉｎ使用６０％甲醇,流速１ｍｌ／ｍｉｎ,０￣９ｍｉｎ２４０ｎｍ紫外检测,：二组分分离良好。各组各组性关系良好,平均回收率氯霉素９９．８％（ＲＳＤ＝１．２％,ｎ＝５）,地塞米松磷酸钠９９．４％（ＲＳＤ＝０．７％,ｎ＝５）,结论：该法用于氯     

Alteration of macrophage anti-tumor activity and transferrin receptor expression by exposure to dimethylnitrosamine in vivo

Dimethylnitrosamine (DMN), a potent immunomodulatory agent, produces its effects on cell-mediated immune reactions through alterations in macrophage production and/or macrophage maturation/differentiation from bone marrow stem cells. Macrophages obtained by the in vitro culture of bone marrow from animals exposed to DMN in vivo (bone marrow-derived macrophages, BMDM) demonstrated enhanced cytotoxicity against L929 target cells. The enhanced cytotoxicity was also present in concanavalin A- and Corynebacterium parvum-elicited peritoneal exudate cells (PEC) obtained from DMN-exposed animals while thioglycollate-elicited PEC from DMN-exposed animals displayed no increase in their cytotoxic activity as compared to vehicle-exposed animals. However, treatment of thioglycollate-elicited PEC with interferon-gamma induced cytotoxic activity in PEC obtained from DMN-exposed animals but not in PEC obtained from control animals. BMDM obtained from DMN-exposed mice also demonstrated an alteration in the kinetics of the expression of the membrane-associated transferrin-binding receptor (mTFR), a marker of the activational status of macrophages. BMDM from animals exposed to DMN in vivo exhibited maximal expression of mTFR on day 7 of culture in vitro as compared to day 5 for BMDM from vehicle-exposed animals. C. parvum- and concanavalin A-elicited PEC from DMN-exposed animals showed dose-related decreases in their expression of mTFR which were associated with their expected enhanced cytotoxicity. Likewise, thioglycollate-elicited PEC from DMN-exposed mice had dose-related decreases in mTFR expression and total transferrin-binding activity, suggesting a change in their state of activation. No alterations in mTFR expression were observed in splenic macrophages. BMDM cultured with T cell-derived lymphokines known to affect mTFR expression demonstrated enhanced expression of mTFR independent of changes in the cell cycle profiles. Furthermore, while lymphokines enhanced mTFR expression, there was no alteration in the kinetics of mTFR expression by BMDM obtained from DMN- or vehicle-exposed animals. These results support the hypothesis that DMN-induced alterations in macrophage hematopoietic differentiation/maturation are manifested in changes in macrophage function.     

BBC TELEVISION SERIES “Let's Go…”: — programmes for teaching the mentally handicapped

A series of 20 programmes designed specifically for the mentally handicapped will be screened by the BBC this Autumn. The following two contributions give an idea of the kind of background work that has taken place.     

The Role of Serotonin (5-HT) in Behavioral Control: Findings from Animal Research and Clinical Implications

The neurotransmitters serotonin and dopamine both have a critical role in the underlying neurobiology of different behaviors. With focus on the interplay between dopamine and serotonin, it has been proposed that dopamine biases behavior towards habitual responding, and with serotonin offsetting this phenomenon and directing the balance toward more flexible, goal-directed responding. The present focus paper stands in close relationship to the publication by Worbe et al. (2015), which deals with the effects of acute tryptophan depletion, a neurodietary physiological method to decrease central nervous serotonin synthesis in humans for a short period of time, on the balance between hypothetical goal-directed and habitual systems. In that research, acute tryptophan depletion challenge administration and a following short-term reduction in central nervous serotonin synthesis were associated with a shift of behavioral performance towards habitual responding, providing further evidence that central nervous serotonin function modulates the balance between goal-directed and stimulus-response habitual systems of behavioral control. In the present focus paper, we discuss the findings by Worbe and colleagues in light of animal experiments as well as clinical implications and discuss potential future avenues for related research.     

The CD11b promoter directs high-level expression of reporter genes in macrophages in transgenic mice [published erratum appears in Blood 1995 Apr 1;85(7):1983]

CD11b is the alpha chain of the Mac-1 integrin and is preferentially expressed in myeloid cells (neutrophils, monocytes, and macrophages). We have previously shown that the CD11b promoter directs cell-type- specific expression in myeloid lines using transient transfection assays. To confirm that these promoter sequences contain the proper regulatory elements for correct myeloid expression of CD11b in vivo, we have used the -1.7-kb human CD11b promoter to direct reporter gene expression in transgenic mice. Stable founder lines were generated with two different reporter genes, a Thy 1.1 surface marker and the Escherichia coli lacZ (beta-galactosidase) gene. Analysis of founders generated with each reporter demonstrated that the CD11b promoter was capable of driving high levels of transgene expression in murine macrophages for the lifetime of the animals. Similar to the endogenous gene, transgene expression was preferentially found in mature monocytes, macrophages, and neutrophils and not in myeloid precursors. These experiments indicate that the -1.7 CD11b promoter contains the regulatory elements sufficient for high-level macrophage expression. This promoter should be useful for targeting heterologous gene expression to mature myeloid cells.