全文获取类型
收费全文 | 19142篇 |
免费 | 1674篇 |
国内免费 | 1419篇 |
专业分类
耳鼻咽喉 | 183篇 |
儿科学 | 146篇 |
妇产科学 | 246篇 |
基础医学 | 2263篇 |
口腔科学 | 213篇 |
临床医学 | 2312篇 |
内科学 | 3384篇 |
皮肤病学 | 188篇 |
神经病学 | 927篇 |
特种医学 | 718篇 |
外国民族医学 | 11篇 |
外科学 | 2049篇 |
综合类 | 3154篇 |
一般理论 | 2篇 |
预防医学 | 1074篇 |
眼科学 | 562篇 |
药学 | 2044篇 |
15篇 | |
中国医学 | 959篇 |
肿瘤学 | 1785篇 |
出版年
2024年 | 63篇 |
2023年 | 317篇 |
2022年 | 744篇 |
2021年 | 952篇 |
2020年 | 673篇 |
2019年 | 647篇 |
2018年 | 725篇 |
2017年 | 572篇 |
2016年 | 607篇 |
2015年 | 852篇 |
2014年 | 1048篇 |
2013年 | 861篇 |
2012年 | 1368篇 |
2011年 | 1507篇 |
2010年 | 856篇 |
2009年 | 718篇 |
2008年 | 1042篇 |
2007年 | 1027篇 |
2006年 | 971篇 |
2005年 | 954篇 |
2004年 | 640篇 |
2003年 | 591篇 |
2002年 | 519篇 |
2001年 | 460篇 |
2000年 | 515篇 |
1999年 | 455篇 |
1998年 | 346篇 |
1997年 | 307篇 |
1996年 | 270篇 |
1995年 | 259篇 |
1994年 | 172篇 |
1993年 | 127篇 |
1992年 | 167篇 |
1991年 | 160篇 |
1990年 | 128篇 |
1989年 | 125篇 |
1988年 | 115篇 |
1987年 | 99篇 |
1986年 | 95篇 |
1985年 | 61篇 |
1984年 | 29篇 |
1983年 | 23篇 |
1982年 | 11篇 |
1981年 | 17篇 |
1980年 | 10篇 |
1979年 | 7篇 |
1978年 | 7篇 |
1977年 | 3篇 |
1976年 | 4篇 |
1974年 | 2篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
81.
我们试验了利用VR技术进行虚拟咬合仿真制作的全部过程。首先,采用光学三维测量仪对上下颌石膏模型进行数字化,通过预处理获取有效的三角网格曲面模型;其次,对咬合运动模型进行合理的简化,分解为一系列的平移运动和旋转运动;通过动态刷新完成开闭口运动、侧移运动的计算机运动仿真,可视化地观察咬合运动;然后利用模型碰撞检测算法动态地计算咬合接触位,并详细地分析了咬合接触时的咬合点位置分布和咬合剖切面上的咬合点接触关系;最后讨论了目前虚拟咬合仿真存在的问题和今后研究的方向。 相似文献
82.
Post-translational modifications of conserved N-terminal tail residues in histones regulate many aspects of chromosome activity. Thr 3 of histone H3 is highly conserved, but the significance of its phosphorylation is unclear, and the identity of the corresponding kinase unknown. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr 3 in prophase and its dephosphorylation during anaphase. Furthermore we find that haspin, a member of a distinctive group of protein kinases present in diverse eukaryotes, phosphorylates H3 at Thr 3 in vitro. Importantly, depletion of haspin by RNA interference reveals that this kinase is required for H3 Thr 3 phosphorylation in mitotic cells. In addition to its chromosomal association, haspin is found at the centrosomes and spindle during mitosis. Haspin RNA interference causes misalignment of metaphase chromosomes, and overexpression delays progression through early mitosis. This work reveals a new kinase involved in composing the histone code and adds haspin to the select group of kinases that integrate regulation of chromosome and spindle function during mitosis and meiosis. 相似文献
83.
Zuochen Du Anwei Chen Lu Huang Xin Dai Qiuyue Chen Di Yang Liling Li Heather Miller Lisa Westerberg Yuan Ding Xuemei Tang Masato Kubo Liping Jiang Xiaodong Zhao Hua Wang Chaohong Liu 《The Journal of allergy and clinical immunology》2021,147(5):1907-1923.e6
- Download : Download high-res image (205KB)
- Download : Download full-size image
84.
Tutulan-Cunita AC Mikoshi M Mizunuma M Hirata D Miyakawa T 《Genes to cells : devoted to molecular & cellular mechanisms》2005,10(5):409-420
Multidrug resistance ABC transporter Pdr5p of Saccharomyces cerevisiae is particularly important due to its ability to export a wide range of unrelated substrates. To clarify its function, we generated Pdr5p mutants by random mutagenesis and screened for mutants with altered drug specificity in vivo by using 5 drug compounds. Nine point mutations that caused significant changes in drug specificity distributed throughout the length of Pdr5p, namely, in the extracellular, transmembrane or cytoplasmic regions of the transporter. We then investigated their effects upon drug resistance, using 36 chemically related or distinct substrates. From this study, overall geometry of the Pdr5p was suggested to contribute in acquiring the enormous range of drug specificity. Based on their ability to inhibit the growth of the mutant strains, the 36 tested drugs were classified into: drugs to which the mutants responded differently (Group 1), drugs to which all the mutants showed sensitivity (Group 2), and drugs to which all the mutants exhibited resistance (Group 3). The ability of the compounds to be partitioned to the plasma membrane seemed an important factor for recognition by Pdr5p. 相似文献
85.
86.
Astrocytes give rise to oligodendrogliomas and astrocytomas after gene transfer of polyoma virus middle T antigen in vivo
下载免费PDF全文
![点击此处可从《The American journal of pathology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Holland EC Li Y Celestino J Dai C Schaefer L Sawaya RA Fuller GN 《The American journal of pathology》2000,157(3):1031-1037
The cells of origin for oligodendrogliomas and astrocytomas are not known but are presumed to be oligodendrocyte and astrocyte precursors, respectively. In this paper we report the generation of mixed gliomas from in vivo transformation of glial fibrillary acidic protein (GFAP)-positive cells (differentiated astrocytes) with polyoma virus middle T antigen (MTA). MTA is a powerful oncogene that activates a number of signal transduction pathways, including those proposed to be involved in gliomagenesis, and has been shown to induce tumors in many cell types. We have achieved transfer of MTA expression specifically to GFAP(+) cells in vivo using somatic cell gene transfer, and find resultant formation of anaplastic gliomas with mixed astrocytoma and oligodendroglioma morphological features. We conclude that GFAP- expressing astrocytes, with appropriate signaling abnormalities, can serve as the cell of origin for oligodendrogliomas, astrocytomas, or mixed gliomas. 相似文献
87.
乳腺富糖原透明细胞癌2例报道及文献复习 总被引:1,自引:1,他引:1
目的 探讨乳腺富糖原透明细胞癌临床病理特点及鉴别诊断要点。方法 对2例乳腺富糖原透明细胞癌进行临床资料及光镜和免疫组化标记观察。结果 组织学特点:癌细胞为多边形或柱状,胞界清楚,胞质透明,呈实性巢状、片状排列,可有乳头形成。表现为导管内癌和浸润性癌结构。免疫组化染色显示:癌细胞呈上皮性免疫表型,CK(AE1)、CEA强阳性,不表达S-100蛋白、肌动蛋白。PAS染色阳性。结论 富含糖原透明细胞癌是上皮性特殊类型乳腺癌,其诊断主要依靠组织病理学和免疫组化标记。 相似文献
88.
Comoviruses are a group of plant viruses in the picornavirus superfamily. The type member of comoviruses, cowpea mosaic virus (CPMV), was crystallized in the cubic space group I23, a = 317 A and the hexagonal space group P6(1)22, a = 451 A, c = 1038 A. Structures of three closely similar nucleoprotein particles were determined in the cubic form. The roughly 300-A capsid was similar to the picornavirus capsid displaying a pseudo T = 3 (P = 3) surface lattice. The three beta-sandwich domains adopt two orientations, one with the long axis radial and the other two with the long axes tangential in reference to the capsid sphere. T = 3 viruses display one or the other of these two orientations. The CPMV capsid was permeable to cesium ions, leading to a disturbance of the beta-annulus inside a channel-like structure, suggesting an ion channel. The hexagonal crystal form diffracted X rays to 3 A resolution, despite the large unit cell. The large ( approximately 200 A) solvent channels in the lattice allow exchange of CPMV cognate Fab fragments. As an initial step in the structure determination of the CPMV/Fab complex, the P6(1)22 crystal structure was solved by molecular replacement with the CPMV model determined in the cubic cell. 相似文献
89.
用PAP法研究亮氨酸脑啡肽样(L—ENK—LI)和P物质样(SP—LI)免疫反应物在大鼠延髓迷走神经背核簇和网状结构内的分布。证明在孤束核、迷走神经背核、外侧网状核外侧部及其外侧的区域内有大量的L-ENK样终末和纤维,在背侧和腹侧网状核之间的移行区内有中等量的分布,其余区内为少量。L-ENK样胞体在孤束核、咀侧腹外侧网状核、巨细胞网状核的腹侧部和α部、外侧旁巨细胞核以及中缝大核内均有许多分布,在迷走神经背核的尾侧部、背侧和腹侧网状核之间的移行区有中等量,其余区内为少量。SP样反应物的分布与L-ENK样物类似,但其终末和纤维的数量较L-ENK者略少,阳性胞体的数量除了在中缝核及外侧旁巨细胞核内侧端中的量较L-ENK样胞体多以外,在其余区内均较少。 相似文献
90.
Differential targets of CpG island hypermethylation in primary and metastatic head and neck squamous cell carcinoma (HNSCC) 总被引:3,自引:0,他引:3
Smiraglia DJ Smith LT Lang JC Rush LJ Dai Z Schuller DE Plass C 《Journal of medical genetics》2003,40(1):25-33
Head and neck squamous cell carcinomas (HNSCC) often metastasise to the cervical lymph nodes. It is known for HNSCC as well as other cancers that progression from normal tissue to primary tumour and finally to metastatic tumour is characterised by an accumulation of genetic mutations. DNA methylation, an epigenetic modification, can result in loss of gene function in cancer, similar to genetic mutations such as deletions and point mutations. We have investigated the DNA methylation phenotypes of both primary HNSCC and metastatic tumours from 13 patients using restriction landmark genomic scanning (RLGS). With this technique, we were able to assess the methylation status of an average of nearly 1300 CpG islands for each tumour. We observed that the number of CpG islands hypermethylated in metastatic tumours is significantly greater than what is found in the primary tumours overall, but not in every patient. Interestingly, the data also clearly show that many loci methylated in a patient's primary tumour are no longer methylated in the metastatic tumour of the same patient. Thus, even though metastatic HNSCC methylate a greater proportion of CpG islands than do the primary tumours, they do so at different subsets of loci. These data show an unanticipated variability in the methylation state of loci in primary and metastatic HNSCCs within the same patient. We discuss two possible explanations for how different epigenetic events might arise between the primary tumour and the metastatic tumour of a person. 相似文献