全文获取类型
收费全文 | 18757篇 |
免费 | 1063篇 |
国内免费 | 150篇 |
专业分类
耳鼻咽喉 | 153篇 |
儿科学 | 375篇 |
妇产科学 | 277篇 |
基础医学 | 2342篇 |
口腔科学 | 564篇 |
临床医学 | 1924篇 |
内科学 | 4051篇 |
皮肤病学 | 385篇 |
神经病学 | 2349篇 |
特种医学 | 1276篇 |
外科学 | 2861篇 |
综合类 | 95篇 |
一般理论 | 7篇 |
预防医学 | 616篇 |
眼科学 | 406篇 |
药学 | 979篇 |
中国医学 | 13篇 |
肿瘤学 | 1297篇 |
出版年
2024年 | 14篇 |
2023年 | 155篇 |
2022年 | 319篇 |
2021年 | 539篇 |
2020年 | 349篇 |
2019年 | 413篇 |
2018年 | 500篇 |
2017年 | 440篇 |
2016年 | 569篇 |
2015年 | 636篇 |
2014年 | 792篇 |
2013年 | 913篇 |
2012年 | 1444篇 |
2011年 | 1438篇 |
2010年 | 949篇 |
2009年 | 866篇 |
2008年 | 1295篇 |
2007年 | 1406篇 |
2006年 | 1179篇 |
2005年 | 1197篇 |
2004年 | 1136篇 |
2003年 | 1047篇 |
2002年 | 938篇 |
2001年 | 162篇 |
2000年 | 124篇 |
1999年 | 165篇 |
1998年 | 165篇 |
1997年 | 128篇 |
1996年 | 103篇 |
1995年 | 71篇 |
1994年 | 66篇 |
1993年 | 65篇 |
1992年 | 47篇 |
1991年 | 37篇 |
1990年 | 22篇 |
1989年 | 33篇 |
1988年 | 36篇 |
1987年 | 19篇 |
1986年 | 21篇 |
1985年 | 17篇 |
1984年 | 16篇 |
1983年 | 18篇 |
1982年 | 14篇 |
1981年 | 8篇 |
1980年 | 11篇 |
1979年 | 10篇 |
1978年 | 16篇 |
1977年 | 13篇 |
1974年 | 7篇 |
1972年 | 7篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
81.
Michael Heming Xiaolin Li Saskia Räuber Anne K. Mausberg Anna-Lena Börsch Maike Hartlehnert Arpita Singhal I-Na Lu Michael Fleischer Fabian Szepanowski Oliver Witzke Thorsten Brenner Ulf Dittmer Nir Yosef Christoph Kleinschnitz Heinz Wiendl Mark Stettner Gerd Meyer zu Hörste 《Immunity》2021,54(1):164-175.e6
82.
83.
H. Heuer Will Spijkers Thomas Kleinsorge Hanno van der Loo Christoph Steglich 《Experimental brain research. Experimentelle Hirnforschung. Expérimentation cérébrale》1998,118(3):381-392
We investigated the time course of the amplitude specification of rapid bimanual reversal movements (lateral displacements
on two digitizers). To this end we used the timed-response paradigm in which the response has to be initiated synchronously
with an auditory signal. Information about the required amplitudes was presented at various times before the synchronization
signal. Consistent with previous results, the progression of amplitude specification was reflected in the dependence of the
amplitudes of the reversal movements on the time interval between amplitude information and synchronization signal. Same or
different amplitudes for the hands were used to examine cross-talk at the programming level of the two-level model of intermanual
interference. The results indicate the existence of cross-talk in particular at short intervals between information about
amplitude and movement initiation. This is consistent with the notion that cross-talk between concurrent processes of amplitude
specification is transient and vanishes as the time available for motor programming increases.
Received: 28 August 1996 / Accepted: 10 July 1997 相似文献
84.
85.
86.
Detection of perforin and granzyme A mRNA in infiltrating cells during infection of mice with lymphocytic choriomeningitis virus 总被引:12,自引:0,他引:12
Christoph Müller David Kgi Toni Aebischer Bernhard Odermatt Werner Held Eckhard R. Podack Rolf M. Zinkernagel Hans Hengartner 《European journal of immunology》1989,19(7):1253-1259
The analysis of gene expression in cytotoxic T cells by in situ hybridization of serial liver and brain sections from mice infected with lymphocytic choriomeningitis virus (LCMV) and immunostaining with T cell marker- and virus-specific antibodies revealed a close histological association of infiltrating lymphocytes expressing the perforin and granzyme A genes with virally infected cells. Maximal frequency of perforin and granzyme A mRNA-containing cells on liver sections preceded by about 2 days maximal LCMV-specific cytotoxicity of the lymphoid liver infiltrating cells. These results are most consistent with an involvement of perforin and granzyme A in cell-mediated cytotoxicity in vivo. 相似文献
87.
Favorably tipping the balance between cytopathic and regulatory T cells to create transplantation tolerance 总被引:23,自引:0,他引:23
Therapeutic application of broadly reactive anti-T cell antibodies can lead not only to potent immunosuppression but also to profound and long-lived T cell depletion. We reasoned that a strategy that almost exclusively targets activated cytopathic donor reactive T cells and spares immunoregulatory networks might prove to be an exceptionally potent and highly selective means of producing long-term engraftment and tolerance. Herein we show that the combined administration of rapamycin and agonist IL-2- and antagonist IL-15-related cytolytic fusion proteins provides for long-term engraftment/tolerance in exceptionally stringent allotransplant models by (1) limiting the early expansion of activated T cells, (2) preserving and even exaggerating their subsequent apoptotic clearance, and (3) further amplifying the depletion of these activated T cells by antibody-dependent mechanisms, while (4) preserving CD4+CD25+ T cell-dependent immunoregulatory networks. 相似文献
88.
Schrader M Krause H Weikert S Straub B Schostak M Christoph F Miller K 《International journal of molecular medicine》2005,16(1):143-147
The aim of our study was to determine whether conventional staging in patients with testicular germ-cell-tumors (GCT) could be supplemented by quantification of beta-human choriogonadotropin mRNA levels in peripheral blood using kinetic fluorescence RT-PCR. Blood samples from 41 patients with GCT of different clinical stages (CS) were pre-therapeutically examined by kinetic fluorescence RT-PCR with the LightCycler for beta-human chorionic gonadotropin (beta-HCG) mRNA expression levels. The controls comprised of samples taken from patients 3 months after treatment, from patients with inflammatory testicular diseases or non-germ-cell-tumors and from healthy males (n=66). Six positive results [cut-off level: normalized beta-HCG mRNA (Nbeta-HCG) >400 relative gene expression (RGE)] were found in controls (specificity 90.9%, 95% CI: 76.9-97.3%). The overall ratio of positive PCR results in the group of GCT patients was 82.92% (34/41) (CS I 18/23, CS IIa-b 6/7, CS >IIb 10/11) (sensitivity 82.9%, 95% CI: 65.1-91.2%). The average Nbeta-HCG level in patients with clinical stage I tumors was 63772.0+/-125720.5 (mean +/- standard deviation) relative gene expression (RGE), 35076.0+/-52253.5 RGE in those with CS IIa-b tumors and 87298.3+/-120895.3 RGE in those with CS >IIb tumors. Kinetic fluorescence RT-PCR for tumor-specific gene products is, in contrast to qualitative RT-PCR, a promising approach to improve conventional staging in clinical low-stage testicular germ-cell-tumors. With high specificity, its sensitivity is higher than that of the corresponding serum tumor marker (82.92% vs 48.72%). 相似文献
89.
90.
Anne Marie-Cardine Henning Kirchgessner Christoph Eckerskorn Stefan C. Meuer Burkhart Schraven 《European journal of immunology》1995,25(12):3290-3297
A glutathione-S-transferase-src-homology domain 2 (GST-SH2) fusion protein was employed to identify molecules interacting with the protein tyrosine kinase p59fyn. Among several proteins which bound to the fyn SH2 domain in lysates of human Jurkat T lymphocytes, α- and β-tubulin were identified by N-terminal sequencing. Further analysis established that α-tubulin exists as a tyrosine-phosphorylated protein in Jurkat cells, where it interacts with p59fyn, but not with p56lck. By contrast, in untransformed resting human T lymphocytes α-tubulin is not detectable as a tyrosine phosphorylated protein. However, following T cell activation, it becomes rapidly phosphorylated on tyrosine residues and subsequently associates with the SH2 domain of fyn. Interestingly, constitutively tyrosine-phosphorylated α-tubulin that is able to interact with the fyn-SH2 domain is expressed in peripheral blood T lymphoblasts isolated from leukemic patients in the absence of external stimulation. 相似文献