脑错构瘤致女婴真性性早熟1例

0　引言　性早熟是指性成熟开始年龄显著提早 ,医学文献具体年限尚无明确界定 ,如按第二性征或月经初潮的平均年龄加减 2个标准差 (x± 2 s)来推断 ,第二性征发育在 9岁以前 ,月经初潮在 10岁以前者被认为是性早熟 . 1999- 0 9- 0 1我们收治脑错构瘤致女婴真性性早熟 1例 ,报道如下 .1　病例报告　女 ,1998- 0 8- 2 5生 ,出生时体质量 2 .1kg,身长48cm,出生后 ,阴道便有白色分泌物流出 ,6 mo时发现与其母同一日阴道出血 ,后成为规律周期性月经 ,时间一般持续 4～ 5 d. 1999- 0 7- 0 1门诊接诊 ,B超检查显示 ,子宫 :轮廓清 ,切面大小约 2 …     

Stimulation of bone formation in the expanding premaxillary suture with a GSK‐3β inhibitor

Oral Diseases (2012) 19 , 73–79 Objectives: Glycogen synthase kinase‐3β (GSK‐3β)/β‐catenin signaling mediates osteogenesis in response to mechanical loading. We tested the hypothesis that local administration of a GSK‐3β inhibitor could stimulate new bone formation in the expanding premaxillary suture. Materials and methods: Thirty‐five Sprague–Dawley rats were subjected to premaxillary suture expansion using a helix spring. The experimental rats were given one or two local injections of SB‐415286, a small‐molecule GSK‐3β inhibitor. Animals were administered calcein and sacrificed on day 7 to quantify new bone formation. To evaluate the proliferation and differentiation of osteoblasts, rats were labeled with bromodeoxyuridine on day 1 and sacrificed on day 2 or 4. β‐catenin expression was evaluated by immunohistochemical staining. Results: Two injections of SB‐415286 led to an elevation of β‐catenin expression and an increase in the number of proliferating osteoblasts in expanding sutures on day 2 and day 4. Consequently, new bone formation in the suture increased significantly on day 7. Conclusions: These results suggest that local delivery of a GSK‐3β inhibitor could stimulate bone formation in the expanding premaxillary suture by eliciting β‐catenin signaling. GSK‐3β could be a pharmaceutical target for improving the effect of orthodontic treatments such as rapid palatal expansion.     

氧氟沙星在胶束溶液中的电分析化学特性

研究了氧氟沙星在胶束溶液中的电分析化学特性,在pH6.30的磷酸盐溶液中,当有0.001%Tween-80存在时,氧氟沙星于-1.46V(vsSCE)产生一灵敏示波极谱波,利用此波可测定10^-8mol·L^-1氧氟沙星。用电毛细曲线、换液试验、循环伏安等方法证实该波为吸附不可逆波,提出了氧氟沙星的电化学反应机理。此方法可用于尿及血清中氧氟沙星测定。相对标准偏差小于7.0%。     

双氯芬酸钠肠溶片质量考察

利用RP-HPLC,以卡马西平(CZ)为内标,对国内3个厂家生产的双氯芬酸钠(D5)肠溶片进行了崩解度、释放度、含量测定的研究。结果表明:不同厂家的DS肠溶片释放度差异极为显著(P<0.01)、崩解度与释放度之间有较好的相关性(r=0.9103)。     

HPLC测定微量小鼠血浆中对乙酰氨基酚的浓度

建立了一种高效液相以谱法测定微量小鼠血浆中对乙酰氨基酚的浓度，采用非那西丁为内标，该法在５．００－１５０．０μｇ／ｍｌ范围内线性相关，方法回收率在９５％以上，日间日内相对标准偏差小于３％，与对乙酰氨基酚相关的６种药物对测定无干扰。     

单疱病毒性角膜炎患者血清一氧化氮的改变及临床意义

1对象和方法 1.1 对象单疱病毒性角膜炎(herpes simplex keratitis,HSK)患者43(男26,女17)例,均系本院收治的患者,其中HSK活动期15例,HSK非活动期28例;发病年龄19～68(平均43.5)岁. 病史、主觉症状和复发诱因,裂隙灯显微镜检查呈典型而特有的病变形态,角膜知觉下降或消失等.     

外伤所致连枷胸102例的护理分析

1临床资料我院1985/2001共收治外伤所致连枷胸患者102(男81,女21)例,男女比例3.9:1.年龄5~70岁,平均46.2岁.致伤原因:交通事故伤75例,高处坠落伤18例,挤压伤9例.开放性损伤22例,闭合性损伤80例.合并伤情况:血胸82例,气胸78例,肺挫伤93例,支气管断裂2例,创伤性窒息8例,脑挫伤5例,硬膜外血肿9例,胸骨骨折3例,四肢骨折40例,脊柱伤10例,脾脏损伤47例,肝脏损伤18例.     

蛋白质定向进化技术在改进单链抗体亲和力方面的研究进展

蛋白质定向进化（DE）在蛋白质工程中取得了令人瞩目的成就,其核心技术——随机突变库构建技术已成为近年来体外DE研究的热点．在对单链抗体进行改进时可以通过引入有性PCR,易错PCR和DNA改组等蛋白质DE技术进行突变和重组,对所得的抗体库进行DE,增加分子遗传多样性,从而提高获得高亲和力、良好稳定性抗体的概率．我们综述了DE的原理和基本方法,并阐述了研究意义和在改进单链抗体亲和力方面的最新研究成果．     

ST segment resolution in patients with tenecteplase-facilitated percutaneous coronary intervention versus tenecteplase alone: Insights from the Combined Angioplasty and Pharmacological Intervention versus Thrombolysis ALone in Acute Myocardial Infarction (CAPITAL AMI) trial

BACKGROUND:

Compared with fibrinolysis alone, fibrinolysis followed by immediate percutaneous coronary intervention (PCI) reduced clinical events in the Combined Angioplasty and Pharmacological Intervention versus Thrombolysis ALone in Acute Myocardial Infarction (CAPITAL AMI) study. It is unclear whether the benefits go beyond achieving epicardial reperfusion.

OBJECTIVES:

To determine the differences in ST segment resolution (STR) among patients treated with tenecteplase (TNK)-facilitated PCI compared with patients treated with TNK alone.

METHODS AND RESULTS:

A formal ST segment analysis was conducted on the 170 patients with ST elevation myocardial infarction in the CAPITAL AMI trial: 86 patients treated with TNK-facilitated PCI were compared with 84 patients who were treated with TNK alone. Epicardial flow measured by percentage with Thrombolysis In Myocardial Infarction (TIMI) 3 flow improved from 52% (pre-PCI) to 89% (post-PCI) in those assigned to facilitated PCI. ST segment resolution was stratified by complete (70% or greater), partial (less than 70% to 30%) or no (less than 30% to 0%) resolution. The baseline mean ST segment elevation was 11.3±7.5 mm in the facilitated PCI patients and 11.8±7.1 mm in patients with TNK alone (P=0.66). Complete STR in the facilitated PCI patients versus the TNK-alone patients was present in 55.6% versus 54.6%, respectively (P=0.58) at 180 min and 62.0% versus 55.3% (P=0.64), respectively at day 1. The mean STR at 180 min and day 1 were similar in patients who experienced death, reinfarction, recurrent unstable ischemia or stroke at six months compared with patients who remained event free: 56.3% versus 64.6% at 180 min (P=0.40); and 67.7% versus 67.6% at day 1 (P=0.99), respectively.

CONCLUSIONS:

TNK-facilitated PCI did not demonstrate differences in ST segment resolution compared with TNK alone, despite improvement in epicardial flow after PCI. Further studies are required to clarify these findings.