防治术后高龄肺癌患者心律失常47例

1 临床资料唐都医院胸腔外科自2000-01/2002-01年间共施行高龄肺癌手术216(男167,女49)例,年龄70～82岁,平均年龄75岁.术前心电图异常42例,低氧血症23例,阻塞性和限制性肺通气功能障碍32例.其中行全肺切除15例,肺叶切除163例,肺楔形切除38例.     

к-硒化卡拉胶对免疫抑制小鼠淋巴细胞增殖及抗体形成细胞的影响(英文)

硒化卡拉胶是一个新的含硒有机化合物。本文主要观察它对环磷酰胺引致免疫抑制小鼠的淋巴细胞增殖及抗体形成细胞(PFC)的影响。研究结果表明,环磷酰胺可明显抑制小鼠淋巴细胞增殖及抗体形成细胞数。硒化卡拉胶2或12 mg/kg po qd×20～40可明显刺激免疫抑制小鼠的淋巴细胞增殖或抗体形成细胞,但大剂量硒化卡拉胶(72 mg/kg)不能显示稳定的刺激作用,在某些条件下反而转向抑制作用。     

芳基异羟肟酸对核苷酸还原酶抑制机制的量子化学研究

应用cNDO/2量子化学方法计算了28个芳基异羟肟酸化合物的电子结构,讨论了异羟肟酸化合物抑制核苷酸还原酶的作用机制。在原络合机制(仅端基部分—CONHOH与酶中金属离子M进行单位点络合)基础上,提出了新的络合机制:当芳环上存在二相邻的—OH或—NH_2时,不仅端基部分能与金属离子M络合,且这二个相邻的—OH(NH_2)中的氧(氮)原子也可以与金属离子M络合,产生双位点络合,提高了化合物抑制核苷酸还原酶的活性。这一新的络合机制合理地解释了原络合机制所无法解释的实验事实。     

Identification of mutations in seven Chinese patients with X-linked chronic granulomatous disease

X-linked chronic granulomatous disease (CGD) is due to mutations in the gp91phox gene on Xp21.1. Studies in white and Japanese X-linked CGD patients have shown mutations in nearly every exon. We studied the molecular defect of seven Chinese patients with X-linked CGD from six unrelated families. Mutations were located by single-strand conformation polymorphism and then defined by sequence analysis. The mutations were two different amino acid substitutions, a nonsense mutation, an in-frame trinucleotide deletion, a single A insertion causing a frameshift, and a premature stop. Lastly, a rare splice site mutation caused by G to A transition at the terminal nucleotide of exon 3, resulting in the skipping of exon 3, was found. The possible effects of these mutations on protein structure-function or splicing were discussed. Together with previous reports, the A insertion in the run of six As from nucleotide 749 to 754 and the G to A transition at the terminal position of exon 3 may be mutation hotspots of the gp91phox gene. The extreme heterogeneous mutations found in our patients suggest the absence of ethnic group-specific mutation.     

Hp疫苗的研制

幽门螺杆菌（Ｈｅｌｉｃｏｂａｃｔｅｒｐｙｌｏｒｉ，Ｈｐ）是一种定植于人胃粘膜的螺杆状微需氧的革兰氏阴性杆菌．Ｈｐ感染属于世界范围性，估计约有半数以上的人口其胃内有Ｈｐ定居．Ｈｐ感染可引起慢性浅表性胃炎、慢性萎缩性胃炎、消化性溃疡、十二指肠球炎和巨大粘...     

前列腺癌中突变型p53蛋白的表达及意义

0 引言 p53蛋白是一个细胞周期相关蛋白,分为野生型(Wtp53)及突变型(Mtp53)两种. 虽然Mtp53蛋白已经丧失抑制细胞增殖的能力,但可能参与调节某些与细胞分化和增殖有关的基因表达. 我们采用组织抗原微波修复技术和免疫组织化学SABC法对前列腺癌(PC)标本中Mtp53蛋白进行检测,探讨PC的病因学、病理学分级、临床分期及其与预后的关系.     

Spatial learning deficits in amyloid precursor protein 770 transgenic mice

Objective To determine whether learning deficits could be seen in transgenic mice expressi ng human amyloid precursor protein 770 (APP770).Methods Female heterozygous transgenic and nontransgenic mice aged 3, 6 and 9 months at the start of testing were used, with eight mice in each age group.All mice wer e subjected to various behavioral tasks including the Y-maze task and the Morri s water maze.After behavioral testing, the mice were sacrificed, and their bra in tissues were used for measuring the choline acetyltransferase (ChAT) activity . Results Nine-month-old transgenic mice exhibited spatial learning deficits in the Morr is water maze and in spontaneous alternation in the Y-maze, compared with those o f the age-matched non-transgenic mice.The behavioral changes accompanied a red uction of ChAT activity in the cortical and hippocampal regions of transgenic mi ce.On the other hand, these behavioral deficits were not observed in transgeni c mice either at 3 or at 6 months of age, in which ChAT activity remained unchan ged. Conclusions The present results show that the learning impairment observed in 9-month-old APP770 transgenic mice are accompanied by a decrease in cortical and hippo campal ChAT activities.This suggests that cholinergic deficits may be involved in the learning impairment observed in these APP770 mice.This model wi ll be a useful tool in advancing our understanding of the relationship between t he cholinergic system and the cognitive deficits observed in Alzheimer’s disease (AD).     

The Platelet Count in Cerebral Malaria,Is It Useful to the Clinician?

We conducted this study to determine the prognostic significance of the platelet count in children with cerebral malaria. We studied children with cerebral malaria admitted to the pediatric research ward at Queen Elizabeth Central Hospital, Malawi. We analyzed 1,811 children with cerebral malaria and compared them with 521 children with bacterial meningitis. There was a significant difference in platelet counts between children with cerebral malaria and those with meningitis. Among children with cerebral malaria, there was no relationship between the platelet count and either the admission coma score or the eventual outcome. Those with malarial retinopathy were more thrombocytopenic than those without. Our results suggest that the platelet count is not prognostic in Malawian children with cerebral malaria.