力学刺激对3月龄骨质疏松大鼠成骨细胞增殖与合成功能的影响

探讨周期性双轴力学应变对成骨细胞增殖与分化合成功能的影响。将正常 3月龄雌性 SD大鼠和骨质疏松大鼠颅顶骨分离的成骨细胞分别在含 10 %胎牛血清的 F- 12培养液中培养 ,并接种在双轴力学应变装置中。当细胞生长至亚融合状态 (Subconfluence) ,给细胞施加力学刺激 ,频率为 1Hz,力学刺激分别为 4 0 0、10 0 0、4 0 0 0μ strain;作用时间分别为每天 30 m in,2、4、8h,共加载两天。以未受力学刺激的细胞为对照组 ,受力学刺激的细胞为实验组 ,并进行比较。采用流式细胞技术测定细胞增殖变化 ;采用同位素标记方法检测成骨细胞骨钙素、I型胶原 C端前肽 (PICP)和总蛋白的分泌量。结果表明 :1)在静态培养条件下 ,3ovx组与 3control组比较 ,其细胞功能活性无明显变化 ,但 3ovx组大鼠成骨细胞增殖活性明显增高 ,这与绝经后骨质疏松骨代谢的高转换率相一致。 2 )4 0 0、10 0 0 μstrain力学刺激可以促进 3control组成骨细胞 I型胶原、骨钙素和总蛋白的分泌量增加 ,促进成骨细胞的分化成熟 ;在 10 0 0μstrain力学刺激下 ,成骨细胞合成骨基质的能力增加最为明显。同时 ,在 4 0 0、10 0 0μstrain力学刺激的初期也可以促进成骨细胞的增殖 ,而促进成骨细胞的分化成熟的作用大于促进细胞增殖的作用。 3)在4 0 0 0 μ     

缺血性卒中或短暂性脑缺血发作患者的卒中预防指南

这份新声明旨在为缺血性卒中或短暂性脑缺血发作存活者的缺血性卒中预防提供全面和及时的循证推荐，循证推荐包括对危险因素的控制，动脉粥样硬化性疾病的干预措施，心源性栓塞的抗栓治疗以及非心源性卒中抗血小板药的应用。另外，还为其他多种特殊情况下复发性卒中的预防提供了推荐、包括动脉夹层分离、卵圆孔未闭、高同型半胱氨酸血症、高凝状态、镰状细胞病、脑静脉窦血栓形成、女性卒中（特别是与妊娠和绝经后激素替代治疗相关卒中），脑出血后肮凝药的应用，以及该指南在高危人群中执行和应用的特殊措施。     

Interaction of misonidazole, hyperthermia, and irradiation in a C3H mammary carcinoma and its surrounding skin in vivo.

The interaction of irradiation, Misonidazole (MISO), and hyperthermia was studied in a C3H mouse mammary carcinoma and its surrounding skin in vivo. MISO (0.5-1.0 mg/g) was injected 30 min before irradiation. Hyperthermia (41.5 degrees-43.5 degrees C for 60 min) was given either simultaneously, 0.5 hr, or 4 hr after X rays. The results were evaluated as the radiation dose to achieve tumor control (TCD50) or moist desquamation of the skin (DD50) in half of the treated animals. A therapeutic gain was found when the enhancement in tumors were greater than that found in skin. The combination of simultaneous heat and irradiation caused great enhancement in radiation response, but with no therapeutic gain. A slightly lower enhancement of the damage in both tissues was found with a 30 min interval between irradiation and hyperthermia, whereas heat 4 hr after X rays gave a small, but significant therapeutic gain. MISO significantly enhanced the response in tumors but not in skin. Combined trimodality treatment with MISO, irradiation, and hyperthermia resulted in enhancement ratios up to 15, dependent on temperature, radiation-heat interval, and to a lesser extent the MISO dose. The enhancement was for all schedules most pronounced in the tumors, resulting in an improved therapeutic effect. The combination of MISO and hyperthermia may be a valuable addition to radiotherapy, especially if heat and irradiation can be applied with close interval and with one of the modalities given selectively to the tumor.     

后颅窝转移瘤

Twenty-seven cases of metastatic tumor of posterior fossa were presented in this paper. Of the patients, 20 were males and 7 females; their age ranged from 20 to 68, and 89% of them were over 40 years of age. The primary foci found were bronchopulmonary carcinomas (7 cases), rhinopharyngeal carcinomas (3 cases), and cancer of the liver (1 case), but in 16 cases the primary foci were not found. All of these patients presented symptoms and signs of obviously increased intracranial pressure, and most of them had the signs of cerebellar and cranial nerves' lesions. Ventriculorgraphy and brain CT scanning were important procedures in the diagnosis, and MRI was most valuable in establishing the diagnosis and localization of posterior fossa metastatic tumors. Twenty-six patients underwent operation. The short-term follow-up study showed an improvement in 25 cases. There was one death. The surgical treatment of posterior fossa metastatic tumor was emphasized. Radiotherapy and chemotherapy were discussed.     

白城市52年间法定报告传染病流行趋势分析

目的：了解掌握法定报告传染病流行情况及变化。方法：采用描述流行病学方法，分析报告传染病的消长分布。结果：传染病的发病率，死亡率，病死率都呈显著下降趋势，病种间消长有明显变化。结论：血源及性传播疾病为新时期的主要传染病病种。     

门诊干扰素治疗慢性乙型病毒性肝炎病人数据库的建立及其应用

报道了应用计算机对感染内科门诊干扰素治疗慢性乙型病毒性肝炎病人的一般资料、干扰素治疗方案、干扰素治疗效果、干扰素副反应、每次复查的生化结果、随访的时间等项目建立数据库,设计程序进行管理。微机能准确地储存资料,方便医护人员对门诊干扰素治疗病人进行及时、有效的治疗和管理,促进病人的健康,提高医护人员的科学管理和社区保健的能力。     

Polymorphisms in ADAM33 are associated with allergic rhinitis due to Japanese cedar pollen

BACKGROUND: A recent report provided evidence that a disintegrin and metalloprotease domain 33 (ADAM33), a member of the ADAM family, is a novel susceptibility gene in asthma linked to bronchial hyper-responsiveness. However, there has been no investigation of the genetic role of ADAM33 variants in nasal allergy. OBJECTIVE: The purpose of this study was to test the association between ADAM33 polymorphisms and Japanese cedar pollinosis (JCPsis), a most common seasonal allergic rhinitis in Japan. METHODS: We conducted a case-control association study among a Japanese population, involving 95 adult individuals with JCPsis and 95 normal healthy controls. A total of 22 single-nucleotide polymorphisms (SNPs) in ADAM33 were genotyped using PCR-based molecular methods. RESULTS: Six SNPs of ADAM33 gene, three in introns (7575G/A, 9073G/A and 12540C/T) and three in the coding region (10918G/C, 12433T/C and 12462C/T), were strongly associated with JCPsis (P = 0.0002-0.022 for absolute allele frequencies) and most of the SNPs were in linkage disequilibrium with each other. A higher frequency of the common alleles of these SNPs was noted for the subjects with JCPsis in comparison with healthy controls. We also identified a haplotype associated with the disease susceptibility. In addition, associations were found between ADAM33 polymorphisms and various cedar pollinosis phenotypes including clinical severity, eosinophil counts in nasal secretion and allergen-specific IgE levels in sera, but not total serum IgE levels. CONCLUSION: These results indicate that polymorphisms in the ADAM33 gene are associated with susceptibility to allergic rhinitis due to Japanese cedar pollen, but the functional relationship still needs clarification.     

经阴道行良性卵巢囊肿剥离术195例报告

目的探讨经阴道行良性卵巢囊肿剥离术的临床实用价值.方法2001年1月2004年6月对有手术指征的良性卵巢囊肿195例实施阴式手术,并观察有关手术指标.结果经阴道完成手术190例,5例因盆腔广泛粘连中转开腹.手术时间45～83 min,平均50 min.术中出血30～90 ml,平均45 ml.术后排气时间4～24 h,平均8.5 h.术后病率18.4%(35/190).183例随访2周内恢复日常家务及工作者分别为72.1%(132/183)、48.1%(88/183).结论经阴道行良性卵巢囊肿剥离术是一种安全、微创的手术.