Differentiation plasticity of human fetal articular chondrocytes.

OBJECTIVE: To test chondrogenic differentiation potential, we examined the differentiation plasticity of isolated human fetal articular chondrocytes (HFACs). STUDY AND DESIGN SETTING: Culture-expanded human fetal articular chondrocytes (HFACs) were analyzed for chondrogenic, adipogenic, osteogenic capacity and neural differentiation ability in defined in vitro culture systems. RESULTS: The different assays demonstrated that culture-expanded HFACs have potential to form cartilage in pellet mass culture, to form adipose cells, osteogenic cells, and neural cells in monolayer culture. CONCLUSIONS: These results suggest that within human fetal articular cartilages there are MSC-like cells that exhibit differentiation plasticity that is comparable with that of BM-MSCs and they may be a new kind of seeding cells for head and neck cartilage reconstruction.     

利培酮治疗与细胞色素P4502D6/C188T酶基因多态性的关联分析

目的　研究利培酮临床效应的个体差异与其代谢酶细胞色素P4 5 0 2D6 (cytochromeP4 5 02D6 ,CYP2D6 )酶基因多态性的相关性。方法　对 88例符合CCMD 3精神分裂症诊断标准的患者和 96例健康对照者作病例 -对照分析。精神分裂症患者给予利培酮治疗 8周 ,用阳性和阴性症状量表 (posi tiveandnegativesymptomscale ,PANSS)评分评价利培酮疗效。采用聚合酶链反应扩增及限制性片段长度多态性 (PCR RFLP)技术对CYP2D6exonⅠ的C188T位点突变进行检测 ,分析利培酮临床效应与其主要代谢酶CYP2D6 /C188T酶基因多态性的相关性。结果　中国上海地区人群的CYP2D6 /C188T突变率(弱代谢型 )为 36 .3% ,病例组和正常对照组间基因型频率总体分布比较无显著差异 (χ2 =1.15 ,df=2 ,P >0 .0 5 ) ,两组间的等位基因频率之间比较也无显著性差异 (χ2 =0 .78,df=1,P >0 .0 5 )。进行性别及有否家族史分组后分析 ,亦无差异存在 ,且CYP2D6 /C188T突变与利培酮临床效应之间并无相关性 (χ2 =1.12 ,df=2 ;χ2 =0 .0 3,df=1,P >0 .0 5 )。结论　未发现中国人CYP2D6 /C188T多态性与利培酮临床效应的个体差异有相关性。     

脐尿管占位性病变的CT诊断及鉴别诊断

目的 探讨CT检查在诊断和鉴别诊断脐尿管占位性病变方面的价值。方法根据临床病理证实的24例脐尿管占位性病变患者的CT影像学特征，分析其大小、部位、形态、性质、Retzius间隙影像学变化及增强后的影像学特征。结果 24例均明确诊断。4例为脐疝，其中3例疝囊内容物为网膜组织，另1例为肠管。20例为脐尿管占位性病变，均位于Retzius间隙内的脐尿管行走区，其中单纯性脐尿管囊肿6例，CT表现为长条椭圆形完整囊状包块，囊壁薄、光滑，囊内容物呈均匀低密度回声，增强后无强化；感染性脐尿管囊肿7例，CT表现为内部回声不均，灶周Retzius间隙内可见絮状或条索状密度增高影，其中4例囊壁明显增厚，3例囊壁不完整，2例呈多房性囊肿；脐尿管憩室伴结石3例，CT表现为中线处膀胱壁内可见高密度结石影；脐尿管肿瘤性病变4例，均于膀胱顶壁区中线处发现不规则软组织肿块影，增强后明显强化。结论 脐尿管占位性病变并不常见，多为感染性囊肿，结合临床术前确诊并不困难。CT是脐尿管占位性病变术前明确诊断和鉴别诊断的重要措施之一。     

Mass and in situ molar activity of tyrosine hydroxylase in the median eminence. Effect of thyroidectomy and thyroid hormone replacement

The effects of thyroidectomy and thyroid hormone replacement on the mass and in situ molar activity of tyrosine hydroxylase (TH) in the median eminence (ME) and superior cervical ganglia (SCG) of male rats were investigated. The tissue specificity of these effects were evaluated by comparing the ME with the superior cervical ganglion (SCG). All animals were thyroparathyroidectomized (Tx) or sham Tx. Tx rats were treated daily for 3 weeks with 0.15 M NaCl (solvent vehicle) or L-thyroxine (T4). Two doses of T4, 10 and 100 micrograms/day/kg BW, were used. Sham Tx rats were treated with 0.15 M NaCl. All animals were studied on the day following the last treatment. The mass of TH was determined using an immunoblot assay, and the in situ activity of TH was calculated from the rate of intracellular accumulation of L-dihydroxyphenylalanine (DOPA) after administration of an inhibitor of DOPA decarboxylase activity. In the ME, thyro-parathyroidectomy resulted in a 40% increase in the mass and a 100% increase in the in situ molar activity of TH over that of sham Tx rats. Compared to Tx animals given 0.15 M NaCl, Tx rats treated with a low dose of T4 (10 micrograms/day/kg BW) had a reduced quantity of TH in the ME, but the molar activity of the enzyme was increased. Treatment of Tx rats with a high dose of T4 (100 micrograms/day/kg BW) restored TH mass but not the in situ activity of TH in the ME to the level seen in sham Tx rats.(ABSTRACT TRUNCATED AT 250 WORDS)     

用酶联免疫吸附术观察向附睾注射鱼肝油酸钠后抗精子抗体的变化

本研究用酶联免疫吸附术观察8只家兔在附睾注射鱼肝油酸钠后血清及精浆中的抗精子抗体。血清抗精子抗体阳性率为37.5～50.0%,最早检出率为术后2个月,6～7月达高峰。精浆抗精子抗体阳性率为25%,最早检出率为术后4个月,且此时己达高峰。与输精管结扎资料相比,发现血清抗精子抗体检出率偏低,而精浆相应抗体偏高,表明向附睾注射鱼肝油酸钠引起的免疫反应有别于结扎,以生殖道局部为主。     

规范化介入治疗方案在肝癌经导管介入治疗中的指导意义

目的探讨规范化介入治疗方案在肝癌经导管介入治疗中的意义。方法回顾性分析实施规范化方案治疗前后患者生存率的变化。结果实施规范化方案后能明显提升患者生存率。结论规范化方案在肝癌经导管介入治疗中具有重要的指导意义。     

AGFA LR5200激光相机的保养与维护

阐述了AGFALR5200激光相机的工作原理、日常使用保养。