大鼠脊髓损伤后白细胞介素-1β、肿瘤坏死因子-α基因表达的变化

目的 探讨大鼠脊髓损伤后白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)mRNA表达的变化规律。方法SD大鼠42只,随机分为7组,采用改良Allen's脊髓损伤打击模型,以逆转录-聚合酶链反应(RT-PCR)法测定伤段脊髓组织IL-1β、TNF-αmRNA的表达情况。结果 正常脊髓组织内存在IL-1β、TNF-αmRNA的表达,脊髓损伤后IL-1β、TNF-αmRNA表达迅速增强,在伤后1h达到高峰。结论 IL-1β、TNF-α存在于正常的脊髓组织内,脊髓损伤后IL-1β、TNF-α表达迅速增强,提示协同参与了继发性脊髓损伤过程,并可能是损伤性因素。     

胸腰椎骨折伴脊髓损伤前后路手术适应证的选择

目的 通过107例胸腰椎骨折伴脊髓损伤病例的治疗,探讨前、后路手术适应证的选择。方法 31例经前路减压植骨,采用Z-Plate等内固定。76例经后路采用短节段椎弓根钉内固定。结果 两组均获得了满意的疗效。结论 选择前或后路手术,应综合考虑脊髓损伤程度、手术时机与技巧、骨折的部位、骨折类型和影像结果等,才能确实做到减压、恢复脊柱序列和坚强固定的目的。     

舰船员慢性荨麻疹与马拉色菌相关性研究

OBJECTIVE: To investigate the association of Malassezia furfur with chronic urticaria in the crew members of ships. METHODS: A comparative mycological study of 126 crew members of ships with chronic urticaria and 45 normal control subjects was carried out. The 82 urticaria patients identified as positive for Malassezia furfur were divided into groups A and B to receive treatment with antihistaminics (group A) and antihistaminics combined with 2% ketoconazole shampoo(group A). RESULTS: The carrier rates of Malassezia furfur were significantly higher in urticaria patients than in the normal control subjects (P<0.01), but in view of the case ratios of the final cure or improvement, no significant difference was observed between the two groups by the end of the treatment courses (P>0.05). But 6 to 8 weeks from the end of the treatment course, better therapeutic effect was noted in group B (P<0.01), with higher rate of negative Malassezia furfur findings (P<0.01). CONCLUSION: Malassezia furfur may play an important role in the prevalence of chronic urticaria among the crew members, and anti-fungal treatment may produce better long-term therapeutic effect.     

三种不同免疫抑制剂对胰岛细胞功能的影响

目的 观察免疫抑制剂对体外培养胰岛细胞活力的影响，探讨免疫抑制剂对胰岛移植物功能的影响。方法 应用体外纯化后培养的SD大鼠胰岛细胞，采用噻唑蓝法测定在不同浓度免疫抑制剂作用下胰岛细胞活力的变化。结果 低剂量雷帕霉素对胰岛细胞活力无明显影响，1ng/ml以上雷帕霉素能引起胰岛细胞活力降低；低剂量和高剂量daclizumab和FTY720对胰岛细胞的活力均无明显影响。结论 雷帕霉素对胰岛细胞有直接损伤作用，这种损伤程度与浓度呈正相关，daclizumab和FTY720对胰岛细胞无明显毒性。     

重症SARS的X线影像表现

目的探讨重症严重急性呼吸综合征(SARS)的X线表现.方法回顾性分析37例重症SARS患者的一系列X线胸片变化规律.结果重症SARS患者胸片表现为两肺阴影迅速进展,大部分区域受累.有3种表现类型:①19例表现为弥漫大片云雾状影,病灶无明显分界,肺纹理显示不清;②13例表现为弥漫棉团状密影,边缘模糊,肺纹理增粗;③5例表现为多发大团块密影,边缘模糊,可见支气管气相.如无合并症发生,对症治疗有效,1个月左右肺部阴影消失.6例肺部合并G-杆菌感染,5例合并纵隔气肿,出现合并症时,肺部阴影延迟吸收.结论重症SARS患者的X线影像为肺部密影快速进展至两肺大部分区域,易合并纵隔气肿及肺部感染.     

异位甲状腺癌的诊断和治疗

目的　探讨异位甲状腺癌的发病特点、诊断和治疗方法。方法　对 2 9例异位甲状腺癌的临床及病理资料进行回顾性分析。结果　 2 9例均行手术治疗 ,术后辅以放、化疗。术后随访 ,5年存活率为 5 2 .0 % ,最长 1例已存活 2 4年。结论　早期发现并及时手术治疗是提高异位甲状腺癌患者生存率的关键 ,术后给予辅助性放、化疗有助于提高生存率。     

Role and regulation of interleukin-1 molecules in pro-asthmatic sensitised airway smooth muscle.

Interleukin (IL)-1beta is a pleiotropic, pro-inflammatory cytokine that has been importantly implicated in driving the inflammatory response and resultant changes in airway smooth muscle (ASM) responsiveness in asthma. IL-1beta belongs to a family of molecules, known as the IL-1 axis, which exert both pro- and anti-inflammatory effects. Since dysregulation of IL-1 axis molecules may be critical in the pathobiology of asthma, the present study examined the expression and activation of both the inhibitory and stimulatory IL-1 axis molecules in human ASM cells and their roles in modulating cytokine and immunoglobulin (Ig)E immune complex (IgE cx)-mediated changes in rabbit ASM constrictor and relaxant responsiveness. The results demonstrate the following. 1) Pre-treatment of isolated rabbit tracheal rings with the inhibitory IL-1 axis members, IL-1 receptor antagonist and IL-1 type-II receptor abrogated both IL-5- and IgE cx-induced changes in ASM responsiveness. 2) Administration of IL-5, IL-1beta and IgE cxs to human ASM cells increased mRNA and protein expressions of both stimulatory and inhibitory IL-1 axis molecules. 3) The time course of IL-5-induced IL-1 axis molecule expression preceded that of both IL-1beta and IgE immune cxs. Collectively, these findings suggest that modulation at the level of the interleukin-1 axis of molecules may have significant therapeutic potential in the treatment of asthma.     

人食管癌组织中热休克蛋白70和糖调节蛋白94的表达

为探讨人食管癌组织中热休克蛋白 70 (HSP70 )和糖调节蛋白 94( grp94)的表达及意义 ,采用SABC法检测人食管癌组织HSP70和grp94的表达 ,镜下观察HSP70和 grp94在癌组织及癌旁组织中的表达特点。结果 :92 .6%( 5 0 /5 4)食管癌组织中存在HSP70的表达 ,癌细胞存在胞质、胞核表达强度及部位的差异 ;而 grp94的表达阳性率为70 .4% ( 3 8/5 4) ,以胞质表达为主 ,而癌旁组织表达较少。提示 :人食管癌组织存在HSP70和 grp94的表达异质性 ,呈现了胞质、胞核的表达差异 ,HSP70和 grp94在人食管癌组织的表达特点为以后肿瘤抗原肽肿瘤疫苗的提取和实验奠定了基础。     

人工心脏瓣膜置换术后PT-INR测定监测口服抗凝药的研究

人工心脏瓣膜置换术后患者口服抗凝治疗不当所致的出血与栓塞占远期并发症的首位。血浆凝血酶原时间（PT）是对人工心脏瓣膜置换术后患者口服抗凝药监测简便、敏感、快速、实用的实验室首选指标，其报告方式的标准化对临床医生有着非常重要的指导意义，国际标准化比率（PT-INR）作为一个较好的监测抗凝药物水平的表示方法，已被WHO推荐使用，但对于人工心脏瓣膜置换术后患者口服抗凝药治疗时PT-INR值的允许范围各地报告不一，为此，我们对我院收治的行人工心脏瓣膜置换术后患者口服华法令治疗时PT监测的情况进行了总结和研究，以探讨适合本地区人工心脏瓣膜置换术后患者口服抗凝药治疗监测中PT-INR的允许范围，为临床合理用药提供安全可靠的实验室监测指标。现报告如下。     

外伤性白内障术后二期人工晶状体植入术

目的 探讨外伤性白内障术后二期植入后房型人工晶状体的时机和手术方式。方法 对34例(34只眼)外伤性白内障术后无晶状体眼根据不同情况采用直接睫状沟植入法或缝线固定法进行二期植入后房型人工晶状体，观察两组患者的术后反应和治疗效果。结果 直接睫状沟植入法14只眼(41．2％)，缝线固定法20只眼(58．8％)，31只眼(91．2％)视力达到0．1以上，21只眼(61．76％)视力达0．3以上，28只眼(82．4％)术后矫正视力等于或高于术前最佳矫正视力。结论 选取合适的时机和方式二期植入人工晶状体，有助于减少术后并发症，提高视功能的恢复。