Cable Pin系统治疗髌骨骨折25例疗效分析

目的探讨Cable Pin系统治疗新鲜移位髌骨骨折的临床疗效。方法自2006年8月至2007年9月采用Cable Pin系统选择性治疗新鲜移位髌骨骨折25例,其中横形骨折20例,粉碎性5例。骨折切开复位后,以细克氏针平行于髌骨关节面纵行预钻孔,连接加压螺钉于动力系统,攻钻入骨。同样方法平行植入第二枚螺钉。于远端横行钻一骨隧道,将钢索穿过隧道,在髌骨前方＂8＂字捆扎。收紧钢索,以束缚器固定。如骨折仍欠稳定可加以环扎固定。结果25例患者随访2～9月（平均6.8月）。骨折均于8～10周内愈合。无螺钉滑脱突出、钢索断裂、滑脱和软组织刺激等并发症发生,远期随访膝关节功能恢复良好。以胥氏综合评分法进行评估：优22例,良3例,优良率100%。结论Cable Pin系统治疗髌骨骨折术后即时稳定性确切,长期随访并发症少,膝关节功能恢复良好。     

Molecular cloning and sequence analysis of the duck enteritis virus UL5 gene

Duck enteritis virus (DEV) is a herpesvirus that causes an acute, contagious, and fatal disease. In the present article, the DEV UL5 gene was cloned and sequenced from a vaccine virus. According to the consensus sequence of herpesvirus UL5 and UL3 gene degenerate oligonucleotide primers were designed and were used in the polymerase chain reaction (PCR) to amplify DNA products with 4577 bp in size. DNA sequence analysis revealed a 2568 bp open reading frame (ORF) encoding a 855 amino acid polypeptide homologous to herpesvirus UL5 proteins. The DEV UL5 gene has a base composition of 769 adenine (29.95%), 556 cytosine (21.65%), 533 guanine (20.76%) and 710 thymine (27.65%). Sequence comparison revealed that the nucleotide sequence of the DEV UL5 gene was highly similar to other alphaherpesviruses. Phylogenetic tree analysis showed that the fifteen herpesviruses viruses analyzed fell into four large groups, and the duck enteritis virus itself branched and was most closely related to meleagrid herpesvirus 1, gallid herpesvirus 2 and gallid herpesvirus 3 subtrees.     

SIGLEC16 encodes a DAP12-associated receptor expressed in macrophages that evolved from its inhibitory counterpart SIGLEC11 and has functional and non-functional alleles in humans

Sialic acid binding immunoglobulin-like lectins (Siglec) are important components of immune recognition. The organization of Siglec genes in different species is consistent with rapid selection imposed by pathogens. We studied SIGLEC11 genes in human, rodent, dog, cow and non-human primates. The lineages of SIGLEC11 genes in these species have undergone dynamic gene duplication and conversion, forming a potential inhibitory (SIGLEC11)/activating (SIGLEC16) receptor pair in chimpanzee and humans. A cDNA encoding human Siglec-16, currently classed as a pseudogene in the databases (SIGLECP16), is expressed in various cell lines and tissues. A polymorphism screen for the two alleles (wild type and four-base pair deletion, 4bpDelta) of SIGLEC16 found their frequencies to be 50% amongst the UK population. A search for donor sequences for SIGLEC16 revealed a subfamily of activating Siglec with charged transmembrane domains predicted to associate with ITAM-encoding adaptor proteins. In support of this, Siglec-16 was expressed at the cell surface in the presence of DAP12, but not the FcRgamma chain. Using antisera specific to the cytoplasmic tail of Siglec-16, we identified Siglec-16 expression in CD14(+) tissue macrophages and in normal human brain, cancerous oesophagus and lung. This is the first activating human Siglec receptor found to have functional and non-functional alleles within the population.     

紫苏对动物微血管的影响和作用机制

实验在6只蟾蜍和19只小白鼠上进行。观察紫苏外用于血管是否有收缩血管的作用。实验结果表明用药后的血管口径与用药前相比，均能明显缩小，有统计意义（p＜0．05～p＜0．01）。这种收缩血管的作用，不为α-受体阻断剂所阻断。此外去鞣酸紫苏和去阳离子紫苏，均能使血管收缩。设想紫苏含有多种活性成分，共同参与血管的收缩作用。     

Genetic isolates in Corsica (France): linkage disequilibrium extension analysis on the Xq13 region

Genetic isolates with a history of a small founder population, long-lasting isolation and population bottlenecks represent exceptional resources in the identification of genes involved in the pathogenesis of multifactorial diseases. In these populations, the disease allele reveals linkage disequilibrium (LD) with markers over significant genetic intervals, therefore facilitating disease locus identification. This study has been designed to examine the background LD extension in some subpopulations of Corsica. Our interest in the island of Corsica is due to its geographical and genetic proximity to the other Mediterranean island of Sardinia. Sardinian isolates in which the extension of the background LD is particularly high have been recently identified and are now the object of studies aimed at the mapping of genes involved in complex diseases. Recent evidence has highlighted that the genetic proximity between the populations of Corsica and Sardinia is particularly true for the internal conservative populations. Given these considerations, Sardinia and Corsica may represent a unique system to carry out parallel association studies whose results could be validated by comparison. In the present study, we have analyzed the LD extension on the Xq13 genomic region in three subpopulations of Corsica: Corte, Niolo and Bozio, all located in the mountainous north-center of the island. Our results show a strong degree of LD over long distance for the population of Bozio and to a less extent for the population of Niolo. Their LD extent is comparable to or higher than that reported for other isolates.     

What's "unstable" in unstable angina?

The role of emotional distress (e.g., anger, depression, and anxiety) in anginal chest discomfort (ACD) may have been underestimated. The authors review the empirical studies in this area, which are inconsistent with the standard theory on the ischemia-angina relationship; summarize the substantial evidence indicating a strong and consistent cross-sectional/prospective epidemiological association of emotional distress and ischemia/ACD; review the distress-targeted, interventional evidence confirming a causal relationship (i.e., reduced chest discomfort and health system utilization), thus confirming clinical utility of such interventions; and explore the possible mechanisms that might account for the relationship between emotional distress and chest discomfort. Substantial clinical benefit may be achieved by aggressively detecting and treating emotional distress in ACD patients.     

一种性能优良的全程控生物电前置放大器

本文介绍了一种性能优良的全程控生物电前置放大器。电路中分别使用模拟开关及开关电容滤波器完成放大器的增益调节和精密低通滤波。该电路除了具有低噪声、高输入阻抗和高共模抑制比外，还具有体积小、可靠性高、性能稳定、无机械噪声等优点，适用于微机化和小型生理信息检测系统。     

病毒性肝炎患者血清IL-6和T细胞亚群的关系

采用ＭＴＴ比色法和抗体致敏的红细胞花环试验（间接法）　检测了９１例病毒性肝炎患者血清ＩＬ－６和Ｔ细胞亚群。　结果显示，病毒性肝炎患者血清ＩＬ－６活性均明显高于正常对照组（Ｐ＜０．０１），以重型肝炎为最高；且与肝细胞损害程度呈正比（Ｐ＜０．０１）；ＣＤ４~＋细胞、ＣＤ４＋／ＣＤ８~＋均明显降低，与血清ＩＬ－６呈负相关；ＣＯ８~＋细胞明显增高，与血清ＩＬ－６呈正相关（Ｐ＜０．０１）。表明，血清ＩＬ－６与机体细胞免疫功能密切相关，二者互为因果，共同参与病毒性肝炎的免疫病理损害，可作为判断病情和预后及免疫调节治疗的监测指标。     

维吾尔、哈萨克及汉族染色体限制酶显带及DA／DAPI二重荧光染色研究

为了解人类染色体中含有高度重复ＤＮＡ的结构异染色质在我国几个民族人群中的分布，作者应用限制性内切酶显带及ＤＡ／ＤＡＰＩ二重荧光染色首次对维吾尔、哈萨克及汉族３０例个体染色体研究发现：（１）ＡｌｕⅠ消化使维吾尔、哈萨克族个体１１，１２号染色体着丝粒Ｃ－带区深染，汉族此两号染色体保持浅染；（２）ＤｄｅⅠ消化使维吾尔、汉族４号染色体为深染，哈萨克族中此号染色体为浅染。限制酶消化后染色差异提示上述几号染色体着丝粒区ＤＮＡ碱基组成有所不同，可用存在两种或两种以上不同结构组分的着丝粒及其在各民族的含量或分布不同来解释；（３）ＤＡ／ＤＡＰＩ二重荧光染色结合ＡｌｕⅠ、ＤｄｅⅠ、ＨａｅⅢ显带表明三民族在某些染色体（１、９、１６－Ｃ带区，１５ｐ、Ｙｑ）异染色质区ＤＮＡ组成是一致的。为人类遗传学的研究提供了资料。     

磷脂酰胆碱抗氧化效应及对遗传损伤的保护作用

磷脂酰胆碱５００ｍｇ／ｋｇ灌胃２０日可使Ｏ_３环境中的小鼠超氧化物歧化酶（ＳＯＤ）活性显著增强，血浆丙二醛（ＭＤＡ）含量及外周血正染红细胞（ＮＣＥ）微核率明显下降。结果提示：磷脂酰胆碱具有抗氧化作用及拮抗自由基造成遗传的损伤。进一步证实了自由基在微核形成中具有重要意义。