PAIP1在肝细胞癌组织中的表达及临床意义

目的检测肝细胞癌组织及肝癌细胞株中PAIP1的表达水平,并分析其表达水平与肝细胞癌的临床病理特征参数的关系。方法采用实时荧光定量PCR(RT-PCR)、WB技术检测新鲜手术标本和肝癌细胞株的PAIP1的表达水平,采用免疫组化方法检测肝癌组织芯片PAIP1蛋白表达水平,比较肝癌组织和癌旁组织PAIP1的表达水平差异,在线工具分析肝癌数据库PAIP1的表达水平与病人临床病理参数的关联性。结果与癌旁组织相比,新鲜标本中癌组织PAIP1 mRNA的表达水平明显增高;PAIP1在肝癌细胞株中高表达;组织芯片中,PAIP1在细胞胞浆中表达,肝癌组织中PAIP1表达水平高于癌旁组织,PAIP1表达水平与与病人生存率呈负相关。结论肝细胞癌中PAIP1高表达,可能在肝细胞癌的发生、发展过程中发挥作用。     

《新型冠状病毒患者标本采集技术专家共识》解读

新型冠状病毒肺炎具有高传染性、长潜伏期、部分临床症状不明显或无症状的特点。确诊或排除新型冠状病毒感染肺炎依赖于对患者生物学标本进行的实验室检测。如何规范、高效、正确、安全地采集新型冠状肺炎相关标本,对提高检测结果的准确率和降低院内感染发生率十分重要,因此本文对《新型冠状病毒患者标本采集技术专家共识》进行解读,以供临床护理人员参考。     

人胚胎发育中期结肠神经系统的研究

目的：观察人胚胎发育中期（4个月－6个月）结肠神经系统的发育。方法：人胚胎结肠切片和铺片，用抗PGP9．5和抗S－100蛋白的PAP免疫组织化学以及NADPHd酶组织化学。结果：①中期结肠肌间神经明显增多，PGP9．5阳性反应神经弥散分布，S－100蛋白反应阳性神经呈竹篓样分布。粘膜下层中阳性神经逐渐分化，形成粘膜下深丛和浅丛。②氮能神经弥散分布于整个结肠壁肌间，环行肌层中阳性神经纤维与平滑肌纤维相平行。在粘膜下深层中偶见氮能核周体。③铺片可见，反应阳性神经纤维形成复杂的肌间神经网络。结论：中期是结肠神经系统发育的重要时期。     

Establishment of an immature mast cell line from a patient with mast cell leukemia

A cell line showing many characteristics of immature mast cells has been established from the peripheral blood of a patient with mast cell leukemia. Cultured cells contain low levels of histamine, are stained metachromatically by toluidine blue, and contain chloroacetate esterase, aminocaproate esterase and tryptase activities. The cells lack T and B lymphocyte, as well as myeloid cell markers, and do not possess IgE receptors. Solid tumors of metachromatically positive cells have been successfully induced and serially passed in nude mice using 5-azacytidine transformed cells. This cell line may be useful for future studies of mast cells and their constituents.     

心肌显像剂[99Tcm(CO)3(MIBI)3]+和99Tcm-MIBI药理实验对比研究

目的对心肌显像剂羰基99Tcm-甲氧基异丁基异腈(MIBI)([99Tcm(CO)3(MIBI)3]+)和99Tcm-MIBI进行动物药理对比研究.方法制备[99Tcm(CO)3(MIBI)3]+注射液,放化纯85%.实验犬3只,每次静脉注射剂量555 MBq,通过动态采集、全身显像和采集血样,获得药代动力学参数、体内生物分布、靶/非靶比值和药效学结果.结果 [99Tcm(CO)3(MIBI)3]+符合一次静脉给药的药代动力学二室模型,快相清除半衰期T(α)1/2=(1.33±0.12) min,慢相清除半衰期T(β)1/2=(102.33±25.58) min,总清除速率(CL)=(401.33±73.51) mL/h.心、肝、肺时间-放射性曲线显示[99Tcm(CO)3(MIBI)3]+肝摄取曲线幅度低于心肌曲线,而99Tcm-MIBI肝曲线明显高于心肌曲线.10、30、60、90和120 min[99Tcm-(CO)3(MIBI)3]+在心、肺、肝百分注射剂量(%ID)与99Tcm-MIBI比较,前者心肌摄取较高,肺本底较低,肝摄取低.[99Tcm(CO)3(MIBI)3]+心/肺和心/肝比值均高于99Tcm-MIBI,差异有显著性,P均<0.01.静脉注射[99Tcm(CO)3(MIBI)3]+后10～120 min均可获得清晰的心肌图像,肝胆排泄较快,肝内放射性较低,左室下壁不受肝影影响,无需脂餐促进肝胆排泄.结论 [99Tcm(CO)3(MIBI)3]+有望成为一种新的心肌灌注显像剂.     

Ku70在卵巢癌组织中的表达及其意义

目的：探讨Ku70在卵巢组织中的表达水平与卵巢癌发生发展的关系。方法：应用免疫组化方法检测Ku70蛋白在15例正常卵巢、20例卵巢囊腺瘤和71例卵巢癌组织中的表达。结果：Ku70在正常卵巢、卵巢囊腺瘤和卵巢癌组织中都有不同程度的表达，但卵巢癌组织中Ku70的表达明显高于正常卵巢组织和卵巢囊腺瘤，且卵巢癌组织分化程度越差则Ku70的阳性率越高，表达强度亦越强，不同组织类型的卵巢癌Ku70的表达无差异。结论：Ku70蛋白可能在卵巢癌的发生发展过程中起重要作用。     

Clinical and employment outcomes of carpal tunnel syndrome in oregon workers’ compensation recipients

We examined demographic, occupational, and treatment characteristics in 316 Oregon workers with carpal tunnel syndrome (CTS) to identify factors associated with clinical and employment outcomes. The goal of this hypothesis-generating study was to identify factors amenable to change through return-to-work programs. Mean claim duration numbered 217 days; median duration was 194 days. Sixty-one percent of workers received timeloss pay at some point during their claim. Workers having CTS surgery numbered 196 (62%). Variables predicting greater days of timeloss in workers with surgery (n=192) included: (1) decreased ability to “get along on income,” (2) attorney involvement, (3) fewer years education, (4) having bilateral surgery, (5) having physical therapy before surgery, (6) longer claim duration, and (7) hand-intensive occupational tasks (r²=36%, adjusted r²=34%). The interplay between occupational and non-occupational factors mandates a comprehensive approach to the evaluation and treatment of workers with CTS.