Immunological and detoxication interaction in p,p-DDT fed rabbits

Summary Thirty-one rabbits were immunized against Salmonella or SRBC. These rabbits had an impaired immunological response when they received 200 ppm p,p-DDT in their drinking water during a period of 38 days. The total gamma globulins as reflected by the 7 S fraction were significantly decreased in the p,p-DDT-Salmonella receiving rabbits when compared with the rabbits receiving only Salmonella. This decrease was not statistically significant in the p,p-DDT-SRBC receiving rabbits when compared with the rabbits receiving only SRBC.The antibody titer against Salmonella was significantly decreased in p,p-DDT-Salmonella receiving rabbits when compared with the rabbits receiving only Salmonella. The decrease of the SRBC antibody titer in the p,p-DDT-SRBC receiving rabbits was not statistically significant.The plasma total DDT level differed significantly in the two groups receiving p,p-DDT (p,p-DDT-Salmonella and p,p-DDT-SRBC receiving rabbits). This finding may explain the different degree of impairment of the immunological response, the higher plasma DDT level having a more marked effect. These differences in total DDT plasma level in the two groups of rabbits which received the same amount of p,p-DDT in their drinking water may be considered as a consequence of the concomitant presence of a different kind of foreign antigen in the internal milieu.The bi-directional relationship between a detoxication process and an immunological response is emphasized.This investigation was supported by a grant from the World Health Organization, International Agency for Research on Cancer, Lyon.     

Treatment outcome of rhabdomyosarcoma in Hong Kong Chinese children.

OBJECTIVES: To review the treatment outcome of rhabdomyosarcoma in Hong Kong Chinese children. DESIGN: Retrospective review. SETTING: University teaching hospital, Hong Kong. PATIENTS: Consecutive cases of rhabdomyosarcoma diagnosed and treated by the Department of Paediatrics and Adolescent Medicine of Queen Mary Hospital between 1989 and 2005. Each patient was staged and treated according to the Intergroup Rhabdomyosarcoma Study guidelines. MAIN OUTCOME MEASURES: Overall and event-free survival rates, and toxicity data. RESULTS: Of 19 patients (8 males and 11 females), 14 (74%) were younger than 10 years old. The median age at diagnosis was 6 (range, 0.5-17) years. Primary sites of rhabdomyosarcoma included: the head and neck (n=8; 6 classified as cranial parameningeal), genitourinary (3), extremity (3), pelvis (3), and trunk (2). Thirteen (68%) had embryonal and six (32%) had alveolar histology. Two, 2, 9, and 6 were classified as belonging to Intergroup Rhabdomyosarcoma Study groups 1, 2, 3, and 4, respectively. Respective 5-year overall and event-free survival rates of the entire cohort were 49% (95% confidence interval, 26-73%) and 32% (10-55%), with a median follow-up of 3.4 (range, 0.2-16.7) years. In non-metastatic cases (Intergroup Rhabdomyosarcoma Study groups 1-3), the 5-year overall survival rate was 66% (95% confidence interval, 39-93%) and in metastatic cases (group 4) it was 17% (0-46%). The 5-year overall survival rate for patients aged less than 10 years was 60% (95% confidence interval, 33-87%) compared to 20% (0-55%) in those aged 10 years and over. Significant treatment-related toxicities including myelosuppression, infections, peripheral neuropathy, and second cancers were encountered. CONCLUSIONS: Treatment outcome of rhabdomyosarcoma in this cohort of Chinese children was less favourable than that reported in international studies. Whilst the main reason could have been related to the high proportion of metastatic cases, also non-metastatic cases faired worse. Improved outcomes may be achieved by advances in multidisciplinary (paediatric oncology, pathology, radiotherapy, and surgery) management and supportive care.     

Sequence of changes in rat buccal mucosa induced by zinc deficiency

In weanling rats, after receiving a zinc-deficient diet (less than 1 ppm) for 4 wk, the buccal mucosa appears hyperplastic. This study determines changes at earlier stages of the lesion. After 9 days of deficiency, the keratin layer had partially converted to parakeratosis and thickened, and the size of the capillary bed was increased. After 18 days, the keratin layer was fully parakeratotic and thickened further. The cellular layer was thickened. The mitotic rate was doubled and rete ridges were convoluted. After 27 days, the keratin and cellular layers were further thickened, and the mitotic rate remained elevated. The rete ridges were further convoluted. The number of mast cells was doubled and the size of the vascular bed had increased further. These findings suggest early and late interactions between the epithelium and lamina propria. After four days on a control diet following 27 days of zinc deficiency, the mucosa returned normal.     

Primacy of existential pain and suffering in residents in chronic pain in nursing homes: a phenomenological study

The study's rationale:  Chronic pain is a major health problem among the elderly, both in the community and within nursing homes.
Aims and objectives:  The purpose of the study was to examine the essentials of the experience of residents in chronic pain in nursing homes.
Methodological design and methods:  The research approach was interpretive phenomenology. Data were collected in 23 dialogues with 12 residents, ages 74–97. Mean age was 86 years.
Results:  The main finding is the primacy of existential pain and suffering in residents in chronic pain in nursing homes. Indeed they recounted a culmination of existential pain and suffering, e.g. loss of loved ones, loss of former home, health and independence, as well as loss of connectedness. Many seemed to be in some kind of grief and their work towards reconciliation to their life and circumstances seemed an ongoing process with successful results while others seemed more haltering or even stuck. Living with chronic pain in a nursing home is indeed a challenge and many blocks to successful pain management were identified. The main sources of strength were loved ones who were seen as lifelines . Nurses seemed distant in their narratives of pain management.
Study limitations:  Frailty of residents is a limitation as a few were starting to forget from one interview to the other and some were actually in pain at the time of our dialogue.
Conclusions:  It is important to support a healthy process of grieving and reconciliation in elderly people who live with chronic pain in nursing homes. Those who suffer in silence with their pain and discomforts and do not seek help should be identified and cared for. The residents' own sources of strength should be identified and supported. Multi-professional collaboration with educational thrust is needed towards quality pain management of elderly people in nursing homes where existential pain and suffering is not excluded.     

髋关节Cam型碰撞综合征髋臼软骨分离的MR关节成像表现

目的回顾性分析MR关节成像显示Cam型髋关节碰撞综合征(FAI)病人髋臼软骨分离的发生率并探讨其价值。材料和方法因为属于回顾性分析,无需获得相关研究委     

Cerebral glucose metabolism during the recovery period after ischemia--its relationship to NADH-fluorescence, blood flow, EcoG and histology

Local cerebral glucose utilization (lCMRgl), NADH fluorescence, cerebral blood flow (CBF), electrocortical activity (ECoG) and histology were studied during a 4 hr recovery period following 2 hrs of left middle cerebral artery (MCA) occlusion in cats. Changes in relative reduced pyridine nucleotides and CBF were measured by fluororeflectometry, ECoG was obtained from the left middle ectosylvian gyrus (MEG), and lCMRgl was measured at the end of the recovery period autoradiographically with 14-C-2-deoxyglucose. A sham group was comprised of 4 cats. The ten animals subjected to the stroke were classified into 3 groups based on the mean amplitude of the ECoG at the end of the ischemic period. At the end of the recovery period, the relative reduced pyridine nucleotides showed a 22.5% oxidation (oxidation of NADH), a 66.2% reduction (reduction of NAD) and a 3.0% reduction compared to the sham group in the severe, moderate and mild groups, respectively. LCMRgl of the left MEG in the severe group was 64.2% of the corresponding sham value, whereas lCMRgl in the moderate and mild groups were 124.8% and 132.0% of the sham, respectively. CBF at the end of the recovery period ranged from 28.1% to 83.0% of the sham value, although there was no significant difference among these groups. Histologically, a large portion of the neurons in the left MEG in the severe group showed ischemic neuronal changes, while the damage was less severe in the moderate and mild groups. On the basis of these data, it is suggested that a relative substrate deficiency and/or a loss of mitochondrial enzymatic pool size may occur in the animals comprizing the severe group. Conversely, anaerobic glycolysis may be activated in the moderate group, while the mild group exhibits an increase in glucose metabolism that is most likely aerobic. A gradient in the magnitude of changes in lCMRgl was noted from the central MCA territory to the surrounding brain regions in the ischemic hemisphere. In addition, there was a mild, but statistically significant (p less than 0.05), depression in lCMRgl with no histological damage in the non-ischemic hemisphere of the severe group.     

The vestibulo-ocular reflex in the cat during linear acceleration in the sagittal plane

The horizontal and vertical components of the vestibulo-ocular reflex (VOR) were recorded in alert cats that were rotated with their head placed on or 45 cm eccentric from the axis of rotation. During off-axis rotation there was a centripetal acceleration along the animal's naso-occipital axis that changed the direction and the magnitude of the resultant otolith force in the animal's sagittal plane. When the animal was upright and eccentric from the axis of rotation, the horizontal VOR (HVOR) had a shorter time constant and smaller amplitude compared to the on-axis HVOR. The effect was symmetrical for both directions of the naso-occipital linear acceleration. When the animal was on its side and faced away from the axis of rotation, there was a decrease in the time constant of the down VOR. When the animal faced the opposite direction, the down VOR time constant was increased. No statistically significant effect was found on the amplitude of the VVOR and the time constant of the up VOR.