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排序方式: 共有6670条查询结果,搜索用时 17 毫秒
151.
Lapierre V Aupérin A Tayebi H Chabod J Saas P Michalet M François S Garban F Giraud C Tramalloni D Oubouzar N Blaise D Kuentz M Robinet E Tiberghien P;Société Française de Greffe de Moelle et de Thérapiue Cellulaire 《Blood》2002,100(4):1484-1489
We have recently shown that the use of allogeneic granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood hematopoietic stem cell transplantation (PBHSCT), as compared with bone marrow transplantation (BMT), is associated with increased titers of antibodies (Abs) directed against red blood cell ABO antigens. To further evaluate the influence of a G-CSF-mobilized PBHSCT graft on alloimmune Ab responses, we examined the frequency of anti-HLA Abs after transplantation in the setting of the same randomized study, comparing PBHSCT with BMT in adults. Anti-HLA Ab presence was determined by complement-dependent cytotoxicity assay (CDC) and flow cytometry in the recipient before and 30 days after transplantation as well as in the donor before graft donation. The use of PBHSCT was significantly associated with increased detection of anti-HLA immunoglobulin G (IgG) Abs early after transplantation as evidenced by flow cytometry (11 of 24 versus 4 of 27 transplant recipients, P =.03) and, less so, by CDC (5 of 24 versus 1 of 27 transplant recipients, P =.09). The difference between PBHSCT and BMT was further heightened when analysis was restricted to anti-HLA IgG Ab-negative donor/recipient pairs. In such a setting, early anti-HLA Ab was never detected after BMT but was repeatedly detected after PBHSCT (flow cytometry, 6 of 18 versus 0 of 17 transplant recipients, P =.02; CDC, 4 of 23 versus 0 of 26 transplant recipients, P =.04). Importantly, the PBHSCT-associated increase in anti-HLA Ab detection was observed despite a reduction in the median number of platelet-transfusion episodes per patient in PBHSC transplant versus BM transplant recipients (3 platelet-transfusion episodes [range, 1-21] in PBHSCT group vs 6 platelet-transfusion episodes [range, 3-33] in the BMT group; P =.02). In conclusion, this study strongly suggests that G-CSF-mobilized PBHSCT results in an increased incidence of circulating anti-HLA Abs and further confirms that the use of such a graft alters alloimmune Ab responses. 相似文献
152.
153.
Wan Ming Da Luc Douay Véronique Barbu Sylvie Fabrega Maria-Alessandra Allieri Xavier Drouet Jacqueline Deloux Marie-Catherine Giarratana Hulya Oszahin Jacqueline van den Akker Danielle Vanhaeke Norbert-Claude Gorin 《British journal of haematology》1991,78(1):42-47
We have previously established a serum-free (SF) culture medium, which supports normal haemopoietic progenitor cell growth for at least 4 weeks as does conventional serum dependent (SD) medium. In the present study, we investigated the efficacy of such a defined SF liquid medium which sustained in vitro residual normal haemopoietic proliferation of marrow derived from ALL patients and which was detrimental for the leukaemic population. Evidence for a potential selective effect of SF culture was obtained by a leukaemic progenitor cell assay (ALL-CFU) and the detection of the bcr/abl translocation by polymerase chain reaction (PCR). In 13 experiments including 12 patients, morphological blast cells and ALL-CFU were dramatically reduced within 3 weeks of incubation in both SF and SD cultures. Likewise, in 5/5 experiments in SD and 2/5 experiments in SF conditions, leukaemic cells expressing the bcr/abl fusion gene disappeared within 3-4 weeks. In contrast, the absolute numbers of supernatant cells harvested weekly from SF and SD cultures were similar. No difference in CFU-GM production was detected for the two culture systems. Erythropoiesis in SF medium exhibited a slower decline than that found in SD. These results indicate that liquid marrow culture may selectively deplete leukaemic lymphoblastic cells and enable repopulation by residual normal haemopoietic cells. This technique may be useful to purge leukaemic cells for clinical autologous bone marrow transplantation in patients with ALL. 相似文献
154.
Dougados M Emery P Lemmel EM de la Serna R Zerbini CA Brin S van Riel P 《The Journal of rheumatology》2003,30(12):2572-2579
OBJECTIVE: The RELIEF investigation was a 48-week, multicenter, international study comprising 2 phases. Results from the first phase, a 24-week open-label cohort study that evaluated the safety and efficacy of leflunomide, as well as predisposing factors to treatment response, are reported here. METHODS: Patients received leflunomide 100 mg once daily for 3 days, followed by 20 mg once daily thereafter. All adverse events were documented. Efficacy variables were the European League Against Rheumatism (EULAR) response criteria using the Disease Activity Score (DAS 28) responder rate and the response rate according to American College of Rheumatology (ACR) criteria. At Week 24, baseline data were analyzed to determine predictive factors for treatment response. RESULTS: A total of 969 patients were entered in the trial. No adverse events that have not previously been seen with leflunomide were reported. Among 968 evaluable patients, 673 (69.6%) completed 24 weeks of treatment and were responders according to DAS 28 response rate, and 587 (60.6%) completed 24 weeks of treatment and were responders according to ACR 20%. Thus, there was a high correlation between the EULAR and ACR criteria in determining treatment response. In addition, 240 (24.8%) patients had a low DAS 28 (< or = 3.2) and 123 (12.7%) patients fulfilled the disease remission criteria (DAS 28 < 2.6) at the end of the study. CONCLUSION: This study demonstrates that leflunomide is well tolerated, with a safety profile similar to that seen previously in Phase III studies, and confirms the efficacy of leflunomide across a range of patient categories. 相似文献
155.
156.
Risk factors of fibrosis in alcohol-induced liver disease 总被引:17,自引:0,他引:17
Raynard B Balian A Fallik D Capron F Bedossa P Chaput JC Naveau S 《Hepatology (Baltimore, Md.)》2002,35(3):635-638
In patients with nonalcoholic steatohepatitis (NASH), age, obesity, and diabetes mellitus are independent predictors of the degree of fibrosis. The relative risk for fibrosis adjusted for sex was also associated with increasing grade of Perls stain. The aim of this study was to determine whether the risk factors for fibrosis described in NASH are also risk factors in alcohol-induced liver disease. A total of 268 alcoholic patients with negative hepatitis B virus and hepatitis C virus serology underwent liver biopsy. Fibrosis was assessed semiquantitatively by a score fluctuating between 0 to 8. Liver iron overload was assessed by Perls staining and graded in 4 classes. We have used multivariate regression with partial correlation analysis to assess the variability of fibrosis score according to the value of 7 variables: sex, age, body mass index (BMI) in the past year before the hospitalization when the patient was asymptomatic, daily alcohol intake over the past 5 years, total duration of alcohol abuse, Perls grade, and blood glucose level. In the multivariate regression, fibrosis score was positively correlated with age (P =.001), BMI (P =.002), female sex (P <.05), Perls grade (P <.05), and blood glucose level (P <.05). Twenty percent of the variability of fibrosis score was explained by the 7 variables. In conclusion, after adjustment for daily alcohol intake and total duration of alcohol abuse, BMI, Perls grade, and blood glucose are also independent risk factors for fibrosis in alcohol-induced liver disease, raising therapeutic implications for the management of these patients. 相似文献
157.
158.
Clinical and molecular delineation of Tetrasomy 9p syndrome: Report of 12 new cases and literature review
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Laïla El Khattabi Sylvie Jaillard Joris Andrieux Laurent Pasquier Laurence Perrin Yline Capri Abdelmadjid Benmansour Annick Toutain Pascale Marcorelles Catherine Vincent‐Delorme Hubert Journel Catherine Henry Claire De Barace Louise Devisme Christèle Dubourg Florence Demurger Josette Lucas Marc‐Antoine Belaud‐Rotureau Jeanne Amiel Valérie Malan Marie‐Christine De Blois Loïc De Pontual Aziza Lebbar Nathalie Le DÛ Dominique P. Germain Jean‐Marc Pinard Eva Pipiras Anne‐Claude Tabet Azzedine Aboura Alain Verloes 《American journal of medical genetics. Part A》2015,167(6):1252-1261
159.
Ian E. Burbulis Kumiko Yamaguchi Olga V. Nikolskaia Sean T. Prigge Stefan Magez Sylvie Bisser Megan E. Reller Dennis J. Grab 《Clinical and Vaccine Immunology : CVI》2015,22(4):374-380
Loop-mediated isothermal amplification (LAMP) is a method for enzymatically replicating DNA that has great utility for clinical diagnosis at the point of care (POC), given its high sensitivity, specificity, speed, and technical requirements (isothermal conditions). Here, we adapted LAMP for measuring protein analytes by creating a protein-DNA fusion (referred to here as a “LAMPole”) that attaches oligonucleotides (LAMP templates) to IgG antibodies. This fusion consists of a DNA element covalently bonded to an IgG-binding polypeptide (protein L/G domain). In our platform, LAMP is expected to provide the most suitable means for amplifying LAMPoles for clinical diagnosis at the POC, while quantitative PCR is more suitable for laboratory-based quantification of antigen-specific IgG abundance. As proof of concept, we measured serological responses to a protozoan parasite by quantifying changes in solution turbidity in real time. We observed a >6-log fold difference in signal between sera from vaccinated versus control mice and in a clinical patient sample versus a control. We assert that LAMPoles will be useful for increasing the sensitivity of measuring proteins, whether it be in a clinical laboratory or in a field setting, thereby improving acute diagnosis of a variety of infections. 相似文献
160.
Yoann Perreux Marie Alexandre Chaix Anna Kamp François-Pierre Mongeon Magali Pham Loïc Boussel Roland Henaine Annie Dore Blandine Mondésert Sylvie Di-Filippo Paul Khairy Francis Bessiere 《Congenital heart disease》2020,15(6):473-482
Sudden cardiac death and heart failure are well known long-term complications after atrial switch for D-transposition of the great arteries (D-TGA).
Right systemic ventricular dysfunction is common and myocardial ischemia has
been implicated as a putative mechanism for sudden death, with coronary anomalies prevalent in 30% of cases. We sought to assess an association between
adverse events and coronary anomalies in patients with D-TGA and atrial switch
surgery. An observational study was conducted in 3 tertiary centers (Montreal
Heart Institute, Canada, Nationwide Children’s hospital, Chicago, USA and Hopital cardiologique Louis Pradel de Lyon, France). Adults with D-TGA and atrial
switch surgery qualified for inclusion if they had a major adverse cardiovascular
event (MACE), i.e., ventricular arrhythmia, sudden cardiac death, heart failure,
cardiac transplantation, or cardiovascular death. The prevalence of coronary
anomalies was compared to historical controls. Forty-five patients were included.
Twenty-one (46.7%) patients experienced a ventricular arrhythmia and 35
(77.8%) suffered from symptomatic heart failure and/or severe right ventricular
dysfunction. Twelve patients (26.7%) had congenitally abnormal coronary
arteries. There was no difference in the prevalence of coronary anomalies between
the cohort with a MACE and a pooled population of 647 historical controls with
D-TGA (28.7%, p = 0.89). In conclusion, the prevalence of congenital coronary
anomalies is not higher in patients with D-TGA and atrial switch surgery who had
adverse cardiovascular events. It could be hypothesized that ischemic complications in this patient population are more likely to be related to a supply-demand
mismatch of the distal microvasculature rather than proximal coronary anomalies. 相似文献