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91.
Daniela Teixeira Joaquim Soares de Almeida Bruna Visniauskas Guiomar Nascimento Gomes Aparecida Emiko Hirata Valquiria Bueno 《Clinics (S?o Paulo, Brazil)》2013,68(6):858-864
OBJECTIVES:
Myeloid-derived suppressor cells contribute to the immunosuppressive microenvironment during tumor development and limit the efficacy of cancer immunotherapy. Identifying myeloid-derived suppressor cells and associated factors is the first step in creating strategies to reverse the suppressive effects of these cells on the immune system.METHODS:
To induce lung cancer, we administered 2 doses of urethane to BALB/c mice and observed these animals for 120 days. After this period, we evaluated the percentage of myeloid-derived suppressor cells in the blood, lung and bone marrow. The expression of alpha-smooth muscle actin, transforming growth factor-β, Toll-like receptor 2, Toll-like receptor 4, and interleukin-6 was also determined in the lung tissue.RESULTS:
Myeloid-derived suppressor cells were increased in all evaluated tissues after lung cancer development in association with increased Toll-like receptor 4 expression and decreased interleukin-6 expression in the lung. We observed alpha-smooth muscle actin and transforming growth factor-β expression in lung nodules.CONCLUSIONS:
We believe that the early diagnosis of cancer through determining the blood levels of myeloid-derived suppressor cells followed by the depletion of these cells should be further investigated as a possible approach for cancer treatment. 相似文献92.
Elizabete Bagordakis Lívia Máris Ribeiro Paranaiba Luciano Abreu Brito Sibele Nascimento de Aquino Ana Camila Messetti Hercílio Martelli‐Junior Mario Sergio Oliveira Swerts Edgard Graner Maria Rita Passos‐Bueno Ricardo D. Coletta DDS MS PhD 《American journal of medical genetics. Part A》2013,161(5):1177-1180
93.
Bueno IA Riccetto AG Morcillo AM Arns CW Baracat EC 《The Brazilian journal of infectious diseases》2012,16(1):86-89
The aims of this study were to determine the presence of respiratory syncytial virus (RSV) and to assess the clinical features of the disease in infants with acute low respiratory tract infection hospitalized at pediatric intensive care units (PICU) of two university teaching hospitals in S?o Paulo State, Brazil. Nasopharyngeal secretions were tested for the RSV by the polymerase chain reaction. Positive and negative groups for the virus were compared in terms of evolution under intensive care (mechanical pulmonary ventilation, medications, invasive procedures, complications and case fatality). Statistical analysis was performed using the Mann Whitney and Fisher's exact tests. A total of 21 infants were assessed, 8 (38.1%) of whom were positive for RSV. The majority of patients were previously healthy while 85.7% required mechanical pulmonary ventilation, 20/21 patients presented with at least one complication, and the fatality rate was 14.3%. RSV positive and negative groups did not differ for the variables studied. Patients involved in this study were critically ill and needed multiple PICU resources, independently of the presence of RSV. Further studies involving larger cohorts are needed to assess the magnitude of the impact of RSV on the clinical evolution of infants admitted to the PICU in our settings. 相似文献
94.
Joanna Wittckind Manoel Gabriele Bordignon Primieri Lívia Maronesi Bueno Nathalie Ribeiro Wingert Ndia Maria Volpato Cssia Virginia Garcia Elfrides Eva Scherman Schapoval Martin Steppe 《RSC advances》2020,10(12):7313
Analysis of impurities is an important step in the quality control of pharmaceutical ingredients and final products. From drug synthesis or excipients, even in small concentrations, impurities may affect efficacy and safety. The method was developed following Quality by Design (QbD) for the analysis of the antidiabetic empagliflozin. The concept of QbD is used as a tool for the development of methods and formulations. Through predefined objectives and risk analysis, robust methodologies and reduced solvent consumption are developed. A simple HPLC method was developed and validated for the quantitative determination of empagliflozin and its organic impurities from the synthesis process. The method was carried out in a Shim-pack phenyl column with a mobile phase consisting of an acetonitrile/water mixture (72 : 28), with isocratic elution and the detector wavelength was 230 nm. The validation process, in accordance with international guidelines, shows that the method was linear, precise and accurate for empagliflozin, impurity 1 and impurity 2. Limits of detection (0.01, 0.02 and 0.01 μg mL−1) and quantification (0.10, 0.10 and 0.05 μg mL−1) were determined for EMPA, IMP1 and IMP2, respectively. The HPLC method for impurity determination in empagliflozin was linear, precise, accurate and robust. It can be successfully applied in the quality control of empagliflozin and the synthesis of impurities, being adequate for routine analysis.Analysis of impurities is an important step in the quality control of pharmaceutical ingredients and final products. 相似文献
95.
96.
J. Rodrigo Diaz‐Siso Ericka M. Bueno Geoffroy C. Sisk Francisco M. Marty Bohdan Pomahac Stefan G. Tullius 《Clinical transplantation》2013,27(3):330-337
Vascularized composite tissue allotransplantation is a viable treatment option for injuries and defects that involve multiple layers of functional tissue. In the past 15 yr, more than 150 vascularized composite allotransplantation (VCA) surgeries have been reported for various anatomic locations including – but not limited to – trachea, larynx, abdominal wall, face, and upper and lower extremities. VCA can achieve a level of esthetic and functional restoration that is currently unattainable using conventional reconstructive techniques. Although the risks of lifelong immunosuppression continue to be an important factor when evaluating the benefits of VCA, reported short‐ and long‐term outcomes have been excellent, thus far. Acute rejections are common in the early post‐operative period, and immunosuppression‐related side effects have been manageable. A multidisciplinary approach to the management of VCA has proven successful. Reports of long‐term graft losses have been rare, while several factors may play a role in the pathophysiology of chronic graft deterioration in VCA. Alternative approaches to immunosuppression such as cellular therapies and immunomodulation hold promise, although their role is so far not defined. Experimental protocols for VCA are currently being explored. Moving forward, it will be exciting to see whether VCA‐specific aspects of allorecognition and immune responses will be able to help facilitate tolerance induction. 相似文献
97.
S. Cantisán R. Lara M. Montejo J. Redel A. Rodríguez‐Benot J. Gutiérrez‐Aroca M. González‐Padilla L. Bueno A. Rivero R. Solana J. Torre‐Cisneros 《American journal of transplantation》2013,13(3):738-745
In this prospective study we analyzed pretransplant interferon‐γ secretion by cytomegalovirus (CMV)‐specific CD8+ T cells to assess its possible utility in determining the risk of CMV replication after solid organ transplantation. A total of 113 lung and kidney transplant patients were enrolled in the study but only 55 were evaluable. All CMV‐seronegative recipients were pretransplant “nonreactive” (IFNγ <0.2 IU/mL) (11/11), whereas 30/44 (68.2%) CMV‐seropositive (R+) recipients were “reactive” (IFNγ ≥0.2 IU/mL) and 14/44 (31.8%) were “nonreactive”. In the R(+) “nonreactive” group, 7/14 (50%) developed posttransplant CMV replication, whereas the virus replicated only in 4/30 (13.3%) of the R(+) “reactive” patients (p = 0.021). According to the best multivariate model, pretransplant “nonreactive” recipients receiving an organ from a CMV‐seropositive donor had a 10‐fold increased risk of CMV replication compared to pretransplant “reactive” recipients (adjusted OR 10.49, 95% CI 1.88–58.46). This model displayed good discrimination ability (AUC 0.80) and calibration (Hosmer–Lemeshow test, p = 0.92). Negative and positive predictive values were 83.7% and 75%, respectively. The accuracy of the model was 82%. Therefore, assessment of interferon‐γ secretion by cytomegalovirus (CMV)‐specific CD8+ T cells prior to transplantation is useful in informing the risk of posttransplant CMV replication in solid organ transplant patients. 相似文献
98.
99.
García-Reyna Benjamín Castillo-García Gilberto Daniel Barbosa-Camacho Francisco José Cervantes-Cardona Guillermo Alonso Cervantes-Pérez Enrique Torres-Mendoza Blanca Miriam Fuentes-Orozco Clotilde Pintor-Belmontes Kevin Josue Guzmán-Ramírez Bertha Georgina Bernal-Hernández Aldo González-Ojeda Alejandro Cervantes-Guevara Gabino 《International journal of mental health and addiction》2022,20(2):895-906
International Journal of Mental Health and Addiction - The presence of COVID-19 has had psychological consequences among health personnel; these include fear, anxiety, and depression. In the... 相似文献
100.
Emily English Megan Solomon Bertha Chen Lisa Rogo-Gupta 《International urogynecology journal》2016,27(12):1905-1911