盐酸地芬尼多片溶出度考察

目的：对盐酸地芬尼多片进行溶出度测定,以考察其质量。方法：采用高效液相色谱法测定了盐酸地芬尼多片的溶出度,色谱柱,YWG－C18柱,流动相：甲醇－0．5％三乙胺溶液（磷酸调PH值为3．5）（62：38）,检测波长;215nm。结果：各（厂）批产品溶出参数（T50,Td,m)有极显著差异（P＜0．01）,结论：有必要增加盐酸地芬尼多片溶出度检查以控制其质量。     

布地奈德都保短期加大剂量吸入治疗急性发作期儿童哮喘52例疗效观察

目的：观察哮喘急性发作期短期加大布地奈德都保用量与短期加用口服或静注糖皮质激素作疗效比较。方法：两组病例均应用常规剂量布地奈德都保,治疗组52例在急性发作期短期加用口服或静脉糖皮质激作比较。结果：两组病例的治疗总有效率均达100％,治疗半年后及治疗1年后的肺功能FEV1,FEF50％（呼气50％FVC时的流速）、PET经统计学处理无明显差异（P＞0．05）。结论：哮喘急性发作期短期加大布地奈德都保用量与短期加用口服或静注糖皮质激素可以取得相同效果。     

A two-locus genetic interaction between LPHN3 and 11q predicts ADHD severity and long-term outcome

The severity of attention-deficit/hyperactivity disorder (ADHD) symptoms is a major predictor of long-term ADHD outcome. To investigate if two-locus interactions might predict ADHD severity, we studied a sample of 1341 individuals from families clustering ADHD, using the Vanderbilt Assessment Scale for Parents. Latent class cluster analysis was used to construct symptom profiles and classify ADHD severity. Single nucleotide polymorphisms (SNPs) spanning ADHD-linked chromosomal regions on chromosomes 4, 5, 10, 11, 12 and 17 were genotyped. SNPs associated with ADHD severity were identified and potential two-locus genetic interactions were tested. We found that SNPs within the LPHN3 gene interact with SNPs spanning the 11q region that contains DRD2 and NCAM1 not only to increase the risk of developing ADHD but also to increase ADHD severity. All these genes are identified to have a major role in shaping both brain development and function. These findings demonstrate that genetic interactions may predict the severity of ADHD, which in turn may predict long-term ADHD outcome.     

The M694V mutation in Armenian‐Americans: a 10‐year retrospective study of MEFV mutation testing for familial Mediterranean fever at UCLA

Familial Mediterranean fever (FMF), inherited in an autosomal recessive manner, is a systemic auto‐inflammatory disorder characterized by recurrent attacks of fever with peritonitis, pleuritis, synovitis and erysipeloid rash. The marenostrin‐encoding fever (MEFV) gene, located on chromosome 16p13.3, is the only gene in which mutations are currently known to cause FMF. To correlate specific genotypes with adverse phenotypes of affected populations residing in the Western United States, a retrospective case series review was conducted of all MEFV gene mutation testing completed at UCLA Clinical Molecular Diagnostic Laboratory between February 2002 and February 2012, followed by clinical chart review of all subjects who either have a single or double mutation. All 12 common mutations in the MEFV gene were analyzed and the M694V variant was found to be associated with an adverse FMF clinical outcome in the Armenian‐American population, manifested by earlier onset of disease, increased severity of disease, and renal amyloidosis.     

Nature of Foetal Haemoglobin in F-Thalassaemia

The nature of Hb-F was studied in 32 heterozygotes for F-thalassaemia, in four homozygotes, and in four persons who have F-thalassaemia in combination with β-thalassaemia or Hb-S. Analysis of the cyanogen bromide fragment γCB-3 indicated that, in all heterozygotes, both ^Gγ and ^Aγ chains were present in Hb-F in an average ratio of about 2:3. In the homozygotes and the double heterozygotes, both ^Gγ and ^Aγ chains were observed in an approximate ratio somewhat higher than 1:1. This pattern of γ chain synthesis is nonspecific for F-thalassaemia but similar to that observed in the traces of Hb-F of normal adults. In conjunctionwith existing information from other genetic studies, it may be concluded that the mutation in F-thalassaemia is associated with a complete deficiency of β and σ chains from cis position together with an increased synthesis of γ chains that is directed by both ^Aγ and ^Gγ loci.     

The impact of preoperative anxiety and education level on long-term mortality after cardiac surgery

Background

Right ventricular failure (RVF) and -support is associated with poor results. We aimed for a new approach of right - sided assistance bypassing the right ventricle and pulmonary circulation in order to better decompress the right ventricle and optimize left ventricular filling.

Methods

From a microaxial pump (Abiomed), a low resistance oxygenator (Maquet and Novalung) and two cannulas (28 and 27 Fr) a system was set up and evaluated in an ovine model (n = 7). Connection with the heart was the right and left atrium. One hour the system was operated without RVF and turned of again. Then a RVF was induced and the course with the system running was evaluated. Complete hemodynamic monitoring was performed as well as echocardiography, flow measurement and blood gas analysis.

Results

The overall performance of the system was reliable. Without RVF no relevant changes of hemodynamics occurred; blood gases were supra normal. In RVF a cardiogenic shock developed (MAP 35 ± 13 mmHg, CO 1,1 ± 0,7 l/min). Immediately after starting the system the circulation normalized (significant increase of MAP to 85 ± 13 mmHg, of CO to 4,5 ± 1,9). Echocardiography also revealed right ventricular recovery. After stopping the system, RVF returned.

Conclusions

Bypassing the right ventricle and pulmonary circulation with an oxygenating assist device, which may offer the advantages of enhanced right ventricular decompression and augmented left atrial filling, is feasible and effective in the treatment of acute RVF. Long time experiments are needed.     

1，1－二烷基－4－（3－溴丙酰基）－哌嗪溴化物的合成及生物活性研究

设计合成了七种新的１，１－二烷基－４－（３－溴丙酰基）哌嗪季铵盐溴化物，通过ＩＲ，￣１ＨＮＭＲ和元素分析证实其结构。初步生物活性试验结果表明，Ⅵａ，Ⅵｃ和Ⅵｅ～ｇ有明显的镇痛活性，Ⅵｅ～ｇ还有较好的抗氧化ＳＯＤ活性，但未发现有抗肿瘤作用。     

腺病毒载体携外源基因转染人培养静脉的实验研究

目的 :探讨经腺病毒载体介导的标记基因转染人培养大隐静脉后 ,标记基因表达的效率、转染靶细胞表达时相。方法 :从临床手术患者取得大隐静脉后 ,浸于含AdCMV/LacZ或AdCMV的病毒液中 (5× 10 9pfu) ,孵育 1h ,将静脉剪成静脉片 ,分别培养 2 ,7,14d。对转基因静脉片进行组织学检查。结果 :腺病毒载体可有效地将标记基因 (LacZ基因 )转入培养大隐静脉。转染后 2d内皮细胞和外膜细胞即有表达 ,β 半乳糖苷酶组织化学染色可见胞核蓝染阳性细胞 ,7d时表达最高 ,持续表达到第 14d。对照大隐静脉无β 半乳糖苷酶表达。 结论 :腺病毒载体能高效转染人大隐静脉 ,其靶细胞以内皮细胞为主 ,外源性基因的表达至少维持 2周左右