全文获取类型
收费全文 | 2531篇 |
免费 | 213篇 |
国内免费 | 6篇 |
专业分类
耳鼻咽喉 | 14篇 |
儿科学 | 84篇 |
妇产科学 | 48篇 |
基础医学 | 335篇 |
口腔科学 | 10篇 |
临床医学 | 247篇 |
内科学 | 502篇 |
皮肤病学 | 42篇 |
神经病学 | 115篇 |
特种医学 | 44篇 |
外科学 | 524篇 |
综合类 | 99篇 |
一般理论 | 2篇 |
预防医学 | 175篇 |
眼科学 | 39篇 |
药学 | 261篇 |
中国医学 | 13篇 |
肿瘤学 | 196篇 |
出版年
2023年 | 24篇 |
2022年 | 27篇 |
2021年 | 82篇 |
2020年 | 76篇 |
2019年 | 72篇 |
2018年 | 58篇 |
2017年 | 73篇 |
2016年 | 83篇 |
2015年 | 103篇 |
2014年 | 104篇 |
2013年 | 137篇 |
2012年 | 191篇 |
2011年 | 212篇 |
2010年 | 126篇 |
2009年 | 89篇 |
2008年 | 171篇 |
2007年 | 157篇 |
2006年 | 137篇 |
2005年 | 138篇 |
2004年 | 96篇 |
2003年 | 92篇 |
2002年 | 65篇 |
2001年 | 50篇 |
2000年 | 36篇 |
1999年 | 41篇 |
1998年 | 16篇 |
1997年 | 24篇 |
1996年 | 9篇 |
1995年 | 12篇 |
1994年 | 9篇 |
1993年 | 7篇 |
1992年 | 22篇 |
1991年 | 13篇 |
1990年 | 17篇 |
1989年 | 17篇 |
1988年 | 11篇 |
1987年 | 13篇 |
1986年 | 11篇 |
1984年 | 10篇 |
1983年 | 10篇 |
1982年 | 7篇 |
1978年 | 9篇 |
1977年 | 6篇 |
1976年 | 10篇 |
1975年 | 6篇 |
1974年 | 9篇 |
1972年 | 11篇 |
1971年 | 7篇 |
1970年 | 6篇 |
1969年 | 6篇 |
排序方式: 共有2750条查询结果,搜索用时 0 毫秒
11.
Immunomodulation of autoimmune and inflammatory diseases with intravenous immunoglobulin 总被引:2,自引:0,他引:2
Ephrem A Misra N Hassan G Dasgupta S Delignat S Duong Van Huyen JP Chamat S Prost F Lacroix-Desmazes S Kavery SV Kazatchkine MD 《Clinical and experimental medicine》2005,5(4):135-140
Abstract Intravenous immunoglobulin (IVIg) has been used in the treatment of primary and secondary antibody deficiencies for over two
decades. Since the early 1980s, the therapeutic efficacy of IVIg has been established in idiopathic thrombocytopenic purpura,
Guillain-Barré syndrome, chronic inflammatory demyelinating polyneuropathy, myasthenia gravis, dermatomyositis and Kawasaki
syndrome, and the prevention of graft versus host disease in recipients of allogeneic bone marrow transplants. Its use has
also been reported in a large number of other autoimmune and systemic inflammatory conditions. In this review, we discuss
the mechanisms by which IVIg exerts immunomodulatory effects in immune pathologies. 相似文献
12.
The pharmacologic activity of fosphenytoin, a new phosphate ester pro-drug of phenytoin, is due to in vivo conversion to phenytoin. Fosphenytoin concentrations cannot be accurately estimated by phenytoin immunoassays (fluorescence polarization and chemiluminescence) owing to the nonlinear relation between fosphenytoin concentration and the observed cross-reactivity. The problem of slow conversion of fosphenytoin to phenytoin in serum in vitro can be circumvented by rapidly converting fosphenytoin to phenytoin in vitro by alkaline phosphatase. Drug-free serum, heparin, EDTA, or citrated plasma were supplemented with 2 concentrations of fosphenytoin. Then to 1-mL aliquots of specimen, no enzyme (control), 10 microL, or 25 microL of enzyme solution was added. The specimens were incubated, and phenytoin concentrations were measured by fluorescence polarization and chemiluminescent assays. In the absence of enzyme, we observed little conversion of fosphenytoin to phenytoin, but in the presence of only 10 microL of enzyme, the conversion of fosphenytoin to phenytoin was complete in 5 minutes. We also observed complete conversion of fosphenytoin to phenytoin by alkaline phosphatase in heparin, EDTA, and citrated plasma. If clinically indicated, the phenytoin concentration can be measured before and after addition of enzyme to roughly estimate the rate of conversion. 相似文献
13.
Characterization of the actions of botulinum neurotoxin type E at the rat neuromuscular junction 总被引:1,自引:0,他引:1
Botulinum neurotoxin (BoTx) serotype E blocks spontaneous and evoked quantal release of acetylcholine at the rat neuromuscular junction. Increasing extracellular Ca2+ to 8 mmol l-1 or substituting Ca2+ with La3+ (0.1 and 1.0 mmol l-1) or depolarizing the nerve terminals by 20 mmol l-1 K+ markedly increases miniature end-plate potential frequency in normal muscle, but in BoTx-E poisoned preparations none of these ions, with the exception of 1 mmol l-1 La3+, was able to restore spontaneous quantal transmitter release to levels recorded at unpoisoned junctions. In absolute values the enhancement with La3+ was much less than that reported at normal junctions. Nerve stimulation in the presence of 3,4-diaminopyridine (10-20 mumol l-1) and high calcium (8 mmol l-1) evoked multiquantal end-plate potentials and muscle twitches. We conclude that the neuromuscular block produced by BoTx serotype E is similar to that previously described for BoTx serotype A but differs from that produced by BoTx serotypes B, D and F in not causing desynchronization of nerve impulse-evoked transmitter release. 3,4-Diaminopyridine might be useful in the treatment of poisoning by BoTx serotype E since it markedly enhanced synchronous transmitter release from poisoned motor nerve terminals. 相似文献
14.
Partha Sarathi Dasgupta Twisha Lahiri 《Journal of cancer research and clinical oncology》1987,113(4):363-368
Summary The cancer chemotherapeutic efficacy of dopamine (DA) was evaluated in female strain A mice bearing transplantable Ehrlich ascites carcinoma. The results demonstrated significant inhibition of tumor growth with appreciable increase in the host survival time following DA treatment. Diminished activity of the growth-related respiratory enzyme succinate dehydrogenase along with stimulated activity of the lysosomal enzyme, -glucuronidase in DA-treated tumor cells indicated inhibition of tumor growth as well as active lysis of the tumor cells. The direct effect of this compound on tumor proliferation was demonstrated by marked inhibition of DNA synthesis. RNA synthesis was only marginally inhibited.Abbreviations DA
Dopamine
- EAC
Ehrlich ascites carcinoma
- SDH
Succinate dehydrogenase
- -Glu
-glueuronidase
- ILS
Increase of life span 相似文献
15.
16.
David K. C. Cooper Hidetaka Hara Hayato Iwase Takayuki Yamamoto Zheng‐Yu Wang Abhijit Jagdale Mohamed H. Bikhet Huy Q. Nguyen Jeremy B. Foote Wayne D. Paris David Ayares Vineeta Kumar Douglas J. Anderson Jayme E. Locke Devin E. Eckhoff 《Clinical transplantation》2021,35(1):e14139
Pig organ xenotransplantation offers a solution to the shortage of deceased human organs for transplantation. The pathobiological response to a pig xenograft is complex, involving antibody, complement, coagulation, inflammatory, and cellular responses. To overcome these barriers, genetic manipulation of the organ‐source pigs has largely been directed to two major aims—(a) deletion of expression of the known carbohydrate xenoantigens against which humans have natural (preformed) antibodies, and (b) transgenic expression of human protective proteins, for example, complement‐ and coagulation‐regulatory proteins. Conventional (FDA‐approved) immunosuppressive therapy is unsuccessful in preventing an adaptive immune response to pig cells, but blockade of the CD40:CD154 costimulation pathway is successful. Survival of genetically engineered pig kidneys in immunosuppressed nonhuman primates can now be measured in months. Non‐immunological aspects, for example, pig renal function, a hypovolemia syndrome, and rapid growth of the pig kidney after transplantation, are briefly discussed. We suggest that patients on the wait‐list for a deceased human kidney graft who are unlikely to receive one due to long waiting times are those for whom kidney xenotransplantation might first be considered. The potential risk of infection, public attitudes to xenotransplantation, and ethical, regulatory, and financial aspects are briefly addressed. 相似文献
17.
Jo-Lynn S. Tan Niranjan Sathianathen Marcus Cumberbatch Prokar Dasgupta Alexandre Mottrie Ronney Abaza Koon Ho Rha Thyavihally B. Yuvaraja Dipen J. Parekh Umberto Capitanio Rajesh Ahlawat Sudhir Rawal Nicolò M. Buffi Ananthakrishnan Sivaraman Kris K. Maes Gagan Gautam Francesco Porpiglia Levent Turkeri Mahendra Bhandari Benjamin Challacombe James Roscoe Porter Craig R. Rogers Daniel A. Moon 《BJU international》2021,128(Z3):30-35
18.
Ari Whiteman Alice Wang Kelly McCain Betsy Gunnels Robin Toblin James Tseryuan Lee Carolyn Bridges Laura Reynolds Bhavini Patel Murthy Judy Qualters James A. Singleton Kimberley Fox Shannon Stokley LaTreace Harris Lynn Gibbs-Scharf Neetu Abad Kathryn A. Brookmeyer Susan Farrall Cassandra Pingali Anita Patel Ruth Link-Gelles Sharoda Dasgupta Radhika Gharpure Matthew D. Ritchey Kamil. E. Barbour 《MMWR. Morbidity and mortality weekly report》2021,70(19):725
19.
20.
K. S. Dasgupta P. S. Mundada Namrata Soni 《Indian journal of otolaryngology and head and neck surgery》2000,52(4):347-349
Fibreoptic bronchoscopy with bronchial aspiration* washings and biopsy was performed in 104 patients suspected clinically
and radio logically of having pulmonary tuberculosis diagnostic yield in 9230% (96/104) cases. Diagnostic yield for tuberculosis
was in 69.22% (72/
104)
cases, it includes positive aspiration and washings smear in 38.46%(40/l04) patients,positive mycobacteriai culture alone in 26.92% (28/104) cases
and
positive biopsy in 3*84% (4/ 104) patients, Non-tuberculous conditions like pneumonia and bronchogenic carcinoma were diagnosed
in 19.23% (20/104) cases and 3.84% (4/104) cases respectively. These results suggest that in areas with high prevalence of
tuberculosis, bronchoscopy should be performed for early diagnosis and initiation of therapy in sputum smear-negative cases
of pulmonary tuberculosis. 相似文献