Neonatal Ostomies

Two methods of neonatal ostomy care, nonappliance and appliance, are discussed. Skin problems associated with stoma care, such as excoriation, fungal infections, and allergic reactions, are reviewed and a number of suggestions are made for the proper care of stomas in the newborn.     

Xenogeneic expression of human stem cell factor in transgenic mice mimics codominant c-kit mutations

Mutations of c-kit, which encodes a transmembrane receptor tyrosine kinase, have been identified in mice by abnormal coat color, anemia, and germ cell defects. Mice heterozygous for mutations of c-kit have a white forehead blaze and a white ventral spot, leading these mutants to be termed dominant White spotting (W). We have previously demonstrated that the membrane-associated isoform of human stem cell factor (hSCF220, the ligand for c-kit) is inefficiently processed in murine stromal cell transfectants. Thus, in murine cell lines analyzed in vitro, hSCF220 transfectants present SCF as a membrane restricted protein in contrast to the murine SCF220 cDNA protein product, which is slowly cleaved and secreted. We show here that transgenic mice expressing the human SCF220 isoform in vivo display a phenotype indistinguishable from some alleles of W. Specifically, hSCF220- expressing transgenic mice display a prominent forehead blaze and a white ventral spot. Generations of doubly heterozygous animals that carry both a mutated c-kit allele and the hSCF220 transgene display a more severe coat color abnormality. This phenotype appears to be due to occupancy of murine c-kit by human SCF and diminished cell surface expression of endogenous murine SCF. Normal signaling events that lead to cell survival or proliferation appear to be disrupted in vivo in these transgenic mice.     

湖北贝母属植物化学成分的研究——Ⅹ.紫花鄂北贝母生物碱的分离与鉴定

紫花鄂北贝母FritiItaria ebeiensis var.pvrpvreaG.D.Yu et P.Li系贝母属植物新变种,从它的鳞茎中分离到Ⅰ～Ⅶ个生物碱。经理化常数测定和波谱分析,并与标准品对照,鉴定碱Ⅰ,Ⅱ,Ⅲ和Ⅳ分别为浙贝甲素(peimine)、浙贝乙素(peiminine)、鄂贝甲素(ebeinine)和鄂贝乙素(ebeinone)。碱Ⅵ为新的异甾生物碱,定名为紫鄂贝碱(ziebeimine),其结构鉴定为5α,14α-cevanine-13,17-dehydro-3α,6β-diol。碱Ⅴ和Ⅶ的结构尚待鉴定。     

一氧化氮代谢物的测定及其与病毒性肝炎若干相关因素的分析

测定了423例各型肝炎、肝炎后肝硬化患者一氧化氮代谢物NO2-／NO3-的值，并与42例正常人作对照，结果肝硬化组NO2-/NO3-值高于对照组及其他各型肝炎组（P＜0．01）。肝硬化NO2-／NO3-的升高与血浆白蛋白（ALB）呈负相关，与腹水量呈正相关，与门脉宽度呈正相关．而与TBA、SB、ALT、AST、凝血酶原活动度等无相关性。提示一氧化氮可能在高动力循环、腹水生成、白蛋白合成方面起重要作用。