胎粪吸入综合征及并发持续动脉高压的心功能变化

探讨胎粪吸入综合征（ＭＡＳ）及并发持续肺动脉高压（ＰＰＨＮ）患儿的心功能变化。应用ＳＳＤ６５０－Ａｌｏｋａ超声多谱勒诊断仪检测４０例正常新生儿和ＭＡＳ患儿的心功能。结果表明：ＭＡＳ患儿左心功能的ＥＦ％（５３．６２）较对照组（６２．１４）,Ａ／Ｅ（０．７６）较对照组（１．１５）均低和右心功能ＣＯＬ／ｍｉｎ（０．８５）较对照组１．１０及,Ａ／Ｅ（０．７８）较对照组１．２２均明显降低,Ｐ均〈０．０５或０     

胸壁转移癌切除后缺损修复一例

胸壁转移癌切除后缺损修复一例王小民＊张付全＊高仕琛＊陈宁＊患者５６岁。３年前患左侧乳癌行扩大根治术，术后创面Ⅰ期愈合。１０个月前同侧胸壁出现一痛性肿块，质硬。检查：左侧胸壁第３，４肋软骨及胸骨旁有一１２ｃｍ×９ｃｍ的不规则肿物，明显突起于皮肤，呈紫红...     

骨髓移植治疗骨不连

目的:探讨骨髓移植在骨不连治疗中的临床疗效。方法:回顾观察1998年9月至2001年10月期间应用自体骨髓移植治疗骨不连的效果。其中男12例,女7例,年龄23～78岁,平均42.5岁。尺骨骨折不愈合2例,股骨干1例,肱骨干6例,胫腓骨9例,桡骨1例。结果:所有病例均得到随访,19例骨不连经过1～3次自体骨髓移植治疗(平均治疗1.9次),均得到治愈,平均愈合时间58天。结论:骨髓移植是治疗骨不连的一种有效手段,可作为以后治疗骨不连的一种选择。     

我国部分省市实验大、小鼠肠道寄生虫检测结果与防控

本文统计和分析了1989年~2013年我国部分省市实验大、小鼠肠道寄生虫病的阳性检测率,统计结果显示,近年来我国部分省市清洁及以上大、小鼠的寄生虫感染状况比过去有所好转,部分地区SPF级实验动物的寄生虫感染率降到10%左右,但对鞭毛虫的控制仍不容乐观。为实验动物生产、管理及使用者提供参考。     

Change of alveolar epithelial type Ⅱ cells and pulmonary surfactant protein A in young rats with acute lung injury

Objective To study the temporal changes of alveolar epithelial type Ⅱ cells and surfactant pro-tein A in young rats with acute lung injury induced by lipopolysaecharide. Method Totally 110 SD young rats (male:53, female : 57) were randomly divided into ALI and normal control groups (six subgroups in each group).LPS(4 mg/kg) was given intraperitoneally in ALI group. The same amount of normal saline was given in the con-trol groups. Eight rats in each subgroup were sacrificed at 6, 12, 24, 36, 48 and 72 hours after the injection.Lung samples were taken for transmission electron microscope examination. RT-PCR was epmloyed for the mea-surement of SP-A mRNA. Western blot was used for the detection of SP-A in the lung tissue. ANOVA and homo-geneity of variance test were performed by SPSS 12.0. Results The microvilli disappeared at 24 hours after the injection of LPS. The number of lamellar body (LBs) was provisionality increased at 24 hours and 48 hours. The ring-like an'angement of LBs around nucleus and the giant LB with vacuole-like deformity were found as the main characteristics of AEC- Ⅱ in ALI at 48 hours. The number of LBs reduced and broken and residual LB remained at 72 hours. SP-A elevated greatly from 24 to 48 hours (P < 0.01), reached peak at 36 hours (6.94 ± 0.80, P <0.01),reached the lowest level(3.87 ±0.50, P <0.01)at 72 hours. Conclusions The pathological changes of AEC-Ⅱ and SP-A in lung tissue wiht ALI are time-dependent. The typical alterations of AEC- Ⅱ occurs at 48 hours accompanied by the compensatory increase of SP-A. AEC- Ⅱ is seriously injuried with the typical changes of LBs and the diminishing of SP-A in lung tissue.     

钛网成形自体颗粒骨修复下颌骨缺损并同期种植的实验研究

目的 观察犬钛网成形自体颗粒骨移植修复下颌骨节段性缺损并同期植入钛种植体后的骨愈合和骨结合情况.方法 5只Beagle犬,一侧下颌骨制备长40 mm的节段性缺损;钛网成形修复下颌骨缺损.将切除后的下颌骨和自体髂骨剪成直径约2mm颗粒,骨皮质、骨松质体积比3∶1混合,紧密充填在钛网内,将2枚纯钛种植体埋置于颗粒骨内,术后6个月处死动物.用下颌骨X线片、组织学切片、扫描电镜以及能谱分析观察钛网内颗粒骨愈合以及种植体骨结合的情况.结果 钛网成形自体颗粒骨移植重建后的下颌骨外形满意,功能正常、颗粒骨成骨良好、结构优良,未见明显骨吸收.同期植入的种植体能够与周围骨组织形成良好的骨结合,并有促进邻近骨组织结构优化的趋势.结论 钛网成形自体颗粒骨移植是一种修复下颌骨节段性缺损的好方法,当修复后下颌骨形态良好、骨质优良、骨量充足时可以同期植入种植体.     

p27在调节小鼠下颌牙槽骨生长发育中的作用

目的明确细胞周期依赖性激酶抑制剂p27在小鼠下颌骨发育形成过程中的作用和机制。方法通过影像学、组织化学染色、免疫组织化学染色等方法比较分析了2周龄p27基因敲除(p27-/-)小鼠与同窝野生型(WT)小鼠下颌牙槽骨的表型差异。取下颌骨提取蛋白,利用Western blot的方法检测了Sonic Hedgehog(Shh)信号通路相关分子表达的变化。结果与同窝2周龄WT小鼠相比,p27-/-小鼠下颌骨体积增大,矿化显著增强;下颌牙槽骨碱性磷酸酶(ALP)阳性面积和I型胶原(Col-Ⅰ)阳性面积的增加,表明p27-/-小鼠成骨细胞活性和功能显著增加。同时发现Shh信号通路相关蛋白Shh、Patched、Smoothened、Gli2的表达水平在p27-/-小鼠均显著上调。结论结果说明细胞周期依赖性激酶抑制剂p27能够通过抑制Shh信号通路,抑制细胞增殖,发挥负性调控小鼠颌骨生长发育的作用。本研究不仅揭示了p27在调控小鼠颌骨生长发育中的作用机制,而且为p27作为调节颌骨生长发育的新靶标提供了理论和实验依据。     

益气通脉解毒方对急性心肌梗死大鼠心肌梗死面积及VEGF和bFGF蛋白表达的影响

目的观察益气通脉解毒方对急性心肌梗死大鼠心肌梗死面积及血管内皮细胞生长因子(VEGF)和碱性成纤维细胞生长因子(b FGF)蛋白表达的影响并探讨其机制。方法采用全扎冠状动脉左前降支法建立急性心肌梗死大鼠模型,随机分为中药组(益气通脉解毒方)、西药组(倍他乐克)与对照组(0.9%氯化钠注射液),4周后检测心功能和心肌梗死面积,HE染色,免疫组化检测VEGF和bFGF蛋白表达。结果 4周后中药组的左室短轴缩短率和左室射血分数高于模型组,左室舒张内径和左室收缩内径低于模型组(P0.05),但与西药组比较无统计学意义(P0.05)。模型组大鼠心肌细胞坏死,细胞核溶解、消失,纤维结缔组织增生,炎症细胞浸润,中药组与西药组也肌细胞坏死减少,纤维组织增生较少,少量炎症细胞浸润。中药组与西药组的心肌梗死面积均显著低于对照组(P0.05),但与西药组比较差异无统计学意义(P0.05)。中药组与西药组的VEGF和bFGF蛋白水平高于模型组(P0.05),但与西药组比较差异无统计学意义(P0.05)。结论益气通脉解毒方可改善急性心肌梗死大鼠局部病理改变及减少纤维化,减少梗死面积,提高VEGF和bFGF蛋白表达,发挥保护心肌损伤作用。     

同一切口行白内障超声乳化与小梁切除术

<正>青光眼与白内障是常见眼病,严重影响视功能,且经常伴发。现将笔者所在医院自2005-01～2006-01采用同一切口行白内障摘除人工晶体植入及小梁切除术27例的临床资料总结如下。     

Change of alveolar epithelial type Ⅱ cells and pulmonary surfactant protein A in young rats with acute lung injury

Objective To study the temporal changes of alveolar epithelial type Ⅱ cells and surfactant pro-tein A in young rats with acute lung injury induced by lipopolysaecharide. Method Totally 110 SD young rats (male:53, female : 57) were randomly divided into ALI and normal control groups (six subgroups in each group).LPS(4 mg/kg) was given intraperitoneally in ALI group. The same amount of normal saline was given in the con-trol groups. Eight rats in each subgroup were sacrificed at 6, 12, 24, 36, 48 and 72 hours after the injection.Lung samples were taken for transmission electron microscope examination. RT-PCR was epmloyed for the mea-surement of SP-A mRNA. Western blot was used for the detection of SP-A in the lung tissue. ANOVA and homo-geneity of variance test were performed by SPSS 12.0. Results The microvilli disappeared at 24 hours after the injection of LPS. The number of lamellar body (LBs) was provisionality increased at 24 hours and 48 hours. The ring-like an'angement of LBs around nucleus and the giant LB with vacuole-like deformity were found as the main characteristics of AEC- Ⅱ in ALI at 48 hours. The number of LBs reduced and broken and residual LB remained at 72 hours. SP-A elevated greatly from 24 to 48 hours (P < 0.01), reached peak at 36 hours (6.94 ± 0.80, P <0.01),reached the lowest level(3.87 ±0.50, P <0.01)at 72 hours. Conclusions The pathological changes of AEC-Ⅱ and SP-A in lung tissue wiht ALI are time-dependent. The typical alterations of AEC- Ⅱ occurs at 48 hours accompanied by the compensatory increase of SP-A. AEC- Ⅱ is seriously injuried with the typical changes of LBs and the diminishing of SP-A in lung tissue.