构建叶酸修饰的乳腺癌靶向纳米超声造影微粒

BACKGROUND: Studies have testified that nano-ultrasound contrast agents have a strong permeability, making it possible to image the targeted tissues outside blood vessels and overcome the limitation that micron contrast agents are only available for the blood pool imaging. OBJECTIVE: To construct the folate-modified nanoparticles targeting breast cancer as ultrasound contrast agents, as well as to observe their ability to specifically bind to cells and imaging effect in vitro. METHODS: Both contrast agents, pegylated lactic acid-glycolic acid copolymer wrapping liquid fluorocarbon formed nanoparticles (mPP/PFOB) and folate modified pegylated lactic acid-glycolic acid wrapping liquid fluorocarbon formed nanoparticles (mPPF/PFOB), were constructed by phacoemulsification-evaporation method. (1)Biocompatibility detection: HFF-1 and MCF-7 cells in the logarithmic phase were cultivated with various concentrations (0, 0.005, 0.01, 0.02, 0.05, 0.1, 0.2 and 1 g/L) of mPP/PFOB or mPPF/PFOB for 24 hours respectively, and then the cell viability was measured. (2)Targeting ability detection in vitro: HFF-1 and MCF-7 cells in the logarithmic phase were divided into three groups. Cy5-labled mPP/PFOB and mPPF/PFOB were added into groups A and B, respectively; the cells in group C were pretreated with folate for 2 hours, and sequentially Cy5-labled mPPF/PFOB was added into group C. Fluorescence intensity was detected by flow cytometry after 0.5 hours of culture. The distribution of contrast agents in cells was observed using confocal microscopy after 20 minutes of culture. (3)Ultrasound imaging in vitro: there were three groups: saline was as group A; the suspension of saline and mPPF/PFOB nanoparticles was prepared as group B; MCF-7 cells were resuspended with the mixture of saline and mPPF/PFOB nanoparticles to prepare the suspension of nanoparticles and cells as group C. In each group, the suspension was added into latex gloves, that were then tightened and immersed in water. Finally, the ultrasound was use to detect the ultrasound imaging effect in vitro. RESULTS AND CONCLUSION: Neither nanoparticles were with significant cytotoxicity. The flow cytometry showed that the mean fluorescence intensity in MCF-7 cells of group B was significantly higher than that of groups A and C. But there were no significant differences in the mean fluorescence intensity in HFF-1 cells among the three groups. It was observed that mPPF/PFOB mainly gathered around the MCF-7 cell membrane, while mPP/PFOB randomly distributed in the cytoplasm. After mPPF/PFOB binding to MCF-7 cells, they could enhance ultrasound echo in vitro. These findings indicate that the targeted nanoparticles mPPF/PFOB have good biocompatibility and can specifically bind to breast cancer MCF-7 cells in vitro and enhance the imaging capability.     

circRNA与子痫前期相关机制的研究进展

子痫前期作为妊娠期疾病,其发病机制尚未完全阐明,尚无可靠的预测及诊断的生物标记物.环状RNA(circRNA)属于内源性非编码RNA分子,其具有高度稳定性与组织特异性等特点,并可充当微小RNA(miRNA)的海绵分子而调控其靶基因表达.近年来研究发现circRNA在子痫前期中表达异常,并可通过miRNA海绵分子的作用从...     

金沉积烤瓷冠单冠修复临床效果观察

目的：探讨金沉积烤瓷冠单冠修复的临床效果。方法选取2010年口腔科门诊收治的80例接受金沉积烤瓷冠单冠修复的患者为研究对象,并根据规定的标准在患者接受修复后的12～24个月内对其实施复检。结果复检80例患者,颜色合格有64例,外形合格70例,边缘合适性患者有64例,牙龈灰线有72例。结论金沉积烤瓷冠单冠修复后的牙齿合格率高、边缘的适合性好,外形颜色也较为美观,不仅起到了修复的作用,还具有一定的美学效果作用。     

安宁疗护在晚期肺癌患者中的应用效果探讨

目的探讨安宁疗护在晚期肺癌患者中的应用效果。方法88例晚期肺癌患者随机分为两组各44例,对照组给予常规护理干预,观察组在对照组基础上给予安宁疗护干预,比较两组干预前后的疼痛程度和负性情绪。结果干预1个月后,两组的VAS评分、SAS评分、SDS评分均显著低于干预前,且观察组的VAS评分、SAS评分、SDS评分均显著低于对照组(P<0.05)。结论安宁疗护可明显减轻晚期肺癌患者的疼痛状况,缓解患者的负性情绪。     

某三甲医院以成本为基础的DRG收付费实践与探索

目的：将2016年某科室DRG成本核算结果与已获得的支付额作对比,得出DRG病种的收益或亏损情况。通过DRG病种核算,找出成本精细化管理的途径和思路。方法：医院利用CN-DRGs医院绩效平台、成本核算系统和数据透视表,分析内分泌科DRGs病种成本。结果：对比DRGs病种成本和已获得支付金额,研究存在的差异,当医院面临实际成本高于病人的DRGs支付价格,医院产生损失;反之,实际成本低于病人的DRGs支付价格,医院获利。结论：医院利用DRG加快完善内部财务管理制度,加强成本核算、分析和控制,降低医疗成本,提高运行效率,同时为政府合理确定DRGs收付费标准提供参考依据。     

人体尿液中曲马多的固相萃取-气相色谱-串联质谱(SPE-GC-MS)定性定量分析方法研究

目的:建立人体尿液中曲马多的SPE-GC-MS定性定量检测分析方法.方法:人体尿液样本采用固相萃取法进行提取,用BSTFA+ 1％ TMCS试剂衍生后,用气相色谱-质谱仪在电子电离模式下检测样本中的曲马多,通过同位素内标方法进行定量分析,从线性、抗干扰性、精密度、回收率及稳定性等方面对方法进行验证;收集临床样本,经本方法同时比对免疫层析法进行临床分析比对.结果:方法的最低检出量为2.0 ng/mL;定量线性范围为75.0～600.0 ng/mL,最低定量限为150.0 ng/mL;在100.0和450.0 ng/mL的浓度下的日内精密度分别为3.90％和1.13％(n=6),日间精密度为1.02％和0.72％(n=6);在150.0和300.0 ng/mL浓度下,前处理回收率分别为95.73％和100.75％(n=3);临床用尿液免疫层析法筛选出来的11份临床阳性尿液样本及1例阴性样本,经本方法及尿液免疫层析法进行检测,结果一致.结论:本方法是一种准确、灵敏、特异性好的检测方法,可用于涉毒人员尿液中曲马多的定性定量分析.     

比较Gd-EOB-DTPA增强MRI圆形感兴趣区与全肝直方图参数预测肝脏局灶性病变肝切除术后肝衰竭的效能

目的比较钆塞酸二钠(Gd-EOB-DTPA)增强MRI T1定量图的圆形感兴趣区均值法和全肝直方图分析参数预测肝脏局灶性病变患者肝切除术后肝衰竭的价值。方法回顾性分析2016年3月至2018年3月因肝脏局灶性病变在复旦大学附属中山医院行Gd-EOB-DTPA增强MRI检查的患者资料。入组40例患者, 其中男性30例, 女性10例, 年龄(56.6±12.1)岁。依据肝切除术后是否发生肝衰竭40例患者分为肝衰竭组(n=14)和对照组(n=26)。比较两组Gd-EOB-DTPA增强前和肝胆特异期(HBP)的T1定量图上圆形感兴趣区以及全肝直方图参数T1值即T1弛豫时间。受试者工作特征(ROC)曲线评估各参数预测术后肝衰竭的价值。结果肝衰竭组直方图参数中T1 HBP均数、T1 HBP标准差、T1 HBP中位数和T1 HBP95%分位数均高于对照组, 差异均有统计学意义(均P<0.05)。圆形感兴趣区3个参数预测肝切除术后肝衰竭效能欠佳(均P>0.05)。在全肝直方图参数中, T1增强前95%分位数预测肝切除术后肝衰竭的ROC曲线下面积为0.702(95%CI:0.523～0.88...     

伊伐布雷定对窄QRS心动过速的疗效评价

目的:探讨伊伐布雷定对窄QRS心动过速患者的治疗效果.方法:选取2018年1月至2020年12月我院收治的103例窄QRS心动过速患者,随机分为两组,对照组51例患者在常规治疗基础上静脉注射普罗帕酮(1～1.5 mg.kg-1加入葡萄糖注射液40 mL),观察组52例患者口服伊伐布雷定5 mg,Bid)进行治疗.治疗4...     

骨科患者围术期预防切口感染的预防护理体会

目的 探究骨科患者切口感染的围术期预防护理方法及效果.方法 将2018年01月～2019年12月收治的的118例四肢骨科手术患者纳入研究,住院期间予抗生素治疗及相应的护理,观察切口感染发生情况,总结护理措施.结果 118例患者共计发生切口感染2例(1.69％),其中Ⅰ类切口1例(0.85％),Ⅱ类切口1例(0.85％)...     

皮肤脂质及其与皮肤健康的关系

皮肤是人体最外层的保护器官,直接与外界环境接触,而包围皮肤的皮脂膜则为皮肤提供屏障保护.皮脂膜由皮脂腺内积聚的皮脂排出皮肤后,与汗腺分泌的汗液及角质细胞崩解产生的脂质,在皮肤角质层表面形成.正常的皮脂膜可滋润皮肤、维持人体皮肤表面常驻菌的生态稳定性、抗感染、提供皮肤屏障、调节并维持皮肤健康.文章简单综述皮肤表面脂质组成...