小肠镜在小肠出血诊断中的应用探讨

回顾性分析62例小肠出血行小肠镜检查患者的临床资料,分为镜检阳性病变检出组（50例）及阴性检出组（12例）,进行临床特征比较。结果显示,阳性病变检出组中75．0％（21／28）的黑便患者经肛侧进镜检出病变,81．8％（18／22）的鲜血便患者经肛侧进镜检出病变（P〈0．01）。阳性病变检出组较阴性检出组病程长,出血次数多,末次出血至小肠镜检查的时间短（P〈0．01或P〈0．05）。出血次数≥3次者阳性检出率显著高于出血次数〈3次者（92．5％比11．1％,P〈0．01）。末次出血后7d内行小肠镜检查阳性检出率明显高于7d后检查者（97．1％比59．3％,P〈0．01）。     

LO2细胞不同缺氧复氧时间细胞存活率检测及瘦素的影响

【目的】复制肝细胞缺血缺氧模型和瘦素对它的影响。【方法】将LO2细胞分别缺氧2h、4h、8h和12h和正常组,后用CCK-8检测各组细胞的A490值,与正常组比较算出细胞的存活率。并用不同浓度的瘦素(分别为100μg/L、200μg/L、400μg/L、800μg/L和1600μg/L)加入缺氧12h的LO2细胞,观察瘦素对其细胞存活率的影响。【结果】LO2细胞经厌氧培养后,缺氧2h、4h、8h和12h细胞CCK-8A490值显著低于缺氧正常组平均值(P<0.01),随着缺氧时间的延长,A490值、细胞存活率逐渐下降;经厌氧培养后的LO2细胞与正常组相比,细胞CCK-8A490值显著低于对照组(P<0.01),不同浓度的LEP能减轻厌氧培养的LO2细胞损伤,A490值、细胞存活增高。【结论】采用厌氧气体培养LO2细胞复制肝脏缺血模型是可行的;瘦素对经厌氧培养后的LO2细胞有保护作用。     

Down-regulation of coxsakie and adenovirus receptor during embryo implantation

In this study,real-time PCR and immunohistochemistry were used to detect coxsakie and adenovirus receptor (CAR) expression.Both localization and quantity were evaluated in the uteri ob-tained at days post coitus (dpc) 2.5,4.5,6.5,8.5.Outcome of PCR was assessed by 2-ΔΔCt method.Im-age Pro-Plus 6.0 software was used for quantifying mean density of CAR expression in immunohisto-chemical sections.We found relatively weak CAR expression in the mouse uteri during implantation window.PCR and immunohistochemistry revealed highest CAR expression was detected on dpc 2.5 followed by down-regulation of CAR at dpc 4.5 and 6.5 (with significant difference).At dpc 8.5,CAR expression was increased slightly again.It is concluded that during implantation,the expression of CAR mRNA and protein is declined,resulting in the impairment of tight junction between cavity epithelium cells.After implantation window closure,CAR appears again to maintain epithelium stability.CAR might play an important role during embryo implantation procedure.     

高效液相色谱法测定复方辛伐他汀烟酸缓释片的有关物质

目的 建立复方辛伐他汀烟酸缓释片有关物质检查的HPLC方法.方法 采用C18柱,以乙腈-0.1%磷酸溶液(50∶50)为流动相A,0.1%磷酸的乙腈溶液为流动相B,梯度洗脱,对辛伐他汀的有关物质进行检测;以乙腈-0.05mol·L-1磷酸二氢钠溶液(磷酸调pH至2.5)(1∶99)为流动相,对烟酸进行检测.结果 在建立的色谱条件下,辛伐他汀、烟酸峰与其相关杂质峰均能完全分离.结论 本法简便、准确、专属性强,可用于本品的有关物质检查.     

盆腔内巨大肠系膜副神经节瘤1例

副神经节瘤是一种少见肿瘤,约5%～10%发生在肾上腺外的部位,常见于Zuckerkandle器(29%)、肠系膜上、下神经节(43%)、纵隔(12%)等部位,发生于肠系膜的副神经节瘤非常罕见,至今国内、外文献报道发生于肠系膜副神经节瘤者仅数例.     

盘龙刺治疗自主神经功能紊乱1例

患者,男,38岁.患者于一月前在开车时突然出现心慌,全身汗出,憋气,呼吸不畅,头晕,遂到急诊治疗,心电图示轻微ST-T改变,未给予针对性治疗,休息后症状缓解.之后半月内又出现两次类似的症状,时感头晕,自汗出,各项检查示无器质性改变.     

综合医院的护理科研管理

护理科研是推动学科发展，提高临床护理质量的重要手段，也是医院护理管理的重要内容。结合综合医院的实际情况，从组织管理、人才管理、经费管理和档案管理等方面分析了护理科研管理的特点，并指出组织管理和人才管理是护理科研管理的核心和重点。     

罕见小肠囊腺瘤1例报道

SUMMARY In recent years, there have been more and more reports about cystadenoma. Cystadenoma can occur in many parts of the body, and cystadenoma in different parts may show different clinical symptoms, however, some patients with cystadenoma have no symptoms. The vast majority of cystadenomas are benign lesions, but a small number of cystadenomas can be malignant. For example, a small number of ovarian cystadenomas and pancreatic cystadenomas may be malignant. This study reported a patient with small intestinal cystadenoma diagnosed by pathology. The patient's physical examination revealed a lesion in the left upper abdomen. He had only abdominal distension and no other discomfort. His laboratory examination results were basically normal, i.e. blood routine, urine routine, stool routine, liver function, kidney function, myocardial enzyme, tumor marker, etc. The patient underwent sectional small intestine resection and the pathological sample was analyzed. The histological findings of the resected intestinal sample were consistent with cystadenoma. Computed tomography scan of the abdomen was performed 4 months after the surgery. No recurrence of the tumor was found. The patient recovered in good condition. By consulting the literature, I found very few reports of small intestinal cystadenoma before, it was very rare. This article described the clinical manifestation, diagnosis and differential diagnosis, treatment and prognosis of a case of small intestinal cystadenoma, it suggested that cystadenoma can occur in the small intestine, other than the ovary, pancreas, liver, lung, thyroid, prostate, seminal vesicle, skin, etc. The cystadenoma in small intestine is easy to be mistaken with other tumors, such as small intestine stromal tumor, small intestine adenocarcinoma, small intestine lipoma, small intestine hemangiomas, etc., and it is difficult to fully confirm through imaging examinations, such as computed tomography and magnetic resonance imaging. Laparotomy and histopathological examination are necessary before definitive diagnosis. This disease can be treated by small bowel resection at the affected region and good prognosis can be achieved.     

科室护士长开展新护士规范化培训实践体验的质性研究

目的:聚焦科室护士长,了解其对新护士规范化培训实践过程中的真实体验,为进一步形成科学的新护士规范化培训方案提供参考。方法:采用便利抽样法,于2020年7—8月选取来自9家大型三甲医院不同临床科室的18名护士长为研究对象进行半结构式访谈,并采用Colaizzi 7步法分析资料。结果:提炼出3个主题：科学的教学实践、积极的...     

标准大骨瓣减压术治疗颅脑外伤的临床效果研究

目的 探讨标准大骨瓣减压术治疗颅脑外伤的临床效果.方法 52例颅脑外伤患者,根据随机数字表法分为标准大骨瓣组和传统对照组,各26例.传统对照组采用传统规骨瓣开颅手术治疗,标准大骨瓣组采用标准大骨瓣减压术治疗.对比两组治疗前后格拉斯哥预后评分、生存质量评分、神经功能评分,治疗效果,并发症发生情况和住院时间.结果 治疗后,...