严重烫伤大鼠早期心肌抑制对肝肾肠损害的影响

Objective To investigate the influence of myocardial inhibition on injury to liver, kid-ney and intestine and blood perfusion at early stage in rat with severe burn. Methods Thirty-two healthy male Wistar rats were enrolled in the study and randomly divided into sham scald, propranolol, scald control and scald + propranolol groups, with 8 rats in each group. After intraperitoneal injection of 10 g/L pentobar-bital sodium for anesthesia, rats of the former two groups were sham scalded in a water bath of 37 ℃ for 18 s, while the latter two groups were inflicted with 30% TBSA full-thickness scald in a 97 ℃ water bath for Parkland formula 30 rains after the injury. At the same time, rats in propranolol and scald + propranolol groups received propranolol 0.75 mg/kg intravenously. After 6 hours, parameters of myocardiac mechanics (SBP, DBP, MAP, LVSP, LVEDP, ± dp/dt max) were recorded by the multiple channel physiological signal collecting and processing system ;blood flow of liver, kidney and intestine were detected with the laser doppler flowmetry;the serum contents of cTnI, TBA, beta2-MG and DAO were determined for reflecting in-juries to the heart, liver, kidney and intestine, respectively. Results Myocardiae mechanics parameters, with the exception of LVEDP, were decreased in propranolol group as compared with those in sham group ( P <0.05). All'myoeardiac mechanics parameters in burn control group were lower than those in sham group and higher than those in burn + propranolol group (P<0.05 ). Blood flow of organs showed similar changes in above-mentioned 3 groups (P<0.05). Organ damages as shown in burn control group [cTnI (4.86+0.29) μg/L, TBA (83.6+18.2) μmol/L, β2-MG (2.75±0.19) mg/L, DAO (1.45±0.09)×103 U/L] were more serious than those in sham control group [ cTnI (1.73±0.09) μg/L, TBA (24.5± 2.4)μmoL/L, β2-MG (1.15±0.18) mg/L, DAO (0.87±0.13)×103U/L] , and less serious than those in scald + propranolol group [cTnl 5.95±0.42 μg/L, TBA 125.8±21.3 μmol/L, β2-MG 3.25 ±0.17 mg/L, DAO (1.83±0.13)×103 U/L ] ( P <0.05 ). Conclusions Propranolol can aggravate injury to the liver, kidney and intestine at early stage in rat with severe burn, suggesting that "shock heart" may be one of initial factors in lowering blood flow to the organs, thus inducing injury to them.     

最大呼气后段流速检测气道阻塞性异常

最大呼气后段流速(FEF75～85%)为最大呼气中段流速(FEF25～75%)之后75～85%部分的呼出气量(L/sec).因处于低肺气量部位,不受主观用力程度的影响,可能较FEF25～75%,及一秒率(FEV1.0%)更力敏感,且可从肺量计描出的用力呼出的时间肺活量曲线上求得,非常简便,极易推广.     

104例糖尿病神经系统病变的临床分析

糖尿病的神经系统病变是糖尿病重要并发症之一,并已日渐引起临床的重视。我们于1981年6～9月对本病患者104例进行了神经系统检查,以期对糖尿病神经病变的早期诊断进行初步探讨。现将结果小结如下:     

大豆异黄酮联合叶酸对胎鼠脑细胞Pax3和Cx43表达的影响

目的观察大豆异黄酮联合叶酸对胎鼠脑细胞Pax3和Cx43表达的调控作用及其与神经管畸形发生的关系,并分析其可能的机制。方法将72只孕鼠随机分为9组对照组、模型组(环磷酰胺,CP组)、叶酸干预组(FA)、大豆异黄酮不同剂量干预组(SIF1、SIF2和SIF3)以及与FA配伍的大豆异黄酮联合干预组(SIF1+FA、SIF2+FA和SIF3+FA)。SP免疫组化法检测胎鼠脑神经细胞Pax3及Cx43蛋白的表达,并观察胎鼠外观,记录胎鼠总数和畸胎数。结果(1)与模型组比较,各干预组均能降低NTDs发生率,其中以FA+SIF2联合干预组效果最好;(2)与对照组比较,模型组中Pax3表达下调,Cx43表达过度;与模型组相比,各干预组均有Pax3表达上调,而Cx43表达下调的改变。结论大豆异黄酮、叶酸或两者联合与NTDs形成相关基因Cx43的下调和Pax3的上调有关;Cx43的过度表达、Pax表达的下调可能是CP诱发NTDs重要机制之一。     

严重烫伤大鼠早期心肌抑制对肝肾肠损害的影响

Objective To investigate the influence of myocardial inhibition on injury to liver, kid-ney and intestine and blood perfusion at early stage in rat with severe burn. Methods Thirty-two healthy male Wistar rats were enrolled in the study and randomly divided into sham scald, propranolol, scald control and scald + propranolol groups, with 8 rats in each group. After intraperitoneal injection of 10 g/L pentobar-bital sodium for anesthesia, rats of the former two groups were sham scalded in a water bath of 37 ℃ for 18 s, while the latter two groups were inflicted with 30% TBSA full-thickness scald in a 97 ℃ water bath for Parkland formula 30 rains after the injury. At the same time, rats in propranolol and scald + propranolol groups received propranolol 0.75 mg/kg intravenously. After 6 hours, parameters of myocardiac mechanics (SBP, DBP, MAP, LVSP, LVEDP, ± dp/dt max) were recorded by the multiple channel physiological signal collecting and processing system ;blood flow of liver, kidney and intestine were detected with the laser doppler flowmetry;the serum contents of cTnI, TBA, beta2-MG and DAO were determined for reflecting in-juries to the heart, liver, kidney and intestine, respectively. Results Myocardiae mechanics parameters, with the exception of LVEDP, were decreased in propranolol group as compared with those in sham group ( P <0.05). All'myoeardiac mechanics parameters in burn control group were lower than those in sham group and higher than those in burn + propranolol group (P<0.05 ). Blood flow of organs showed similar changes in above-mentioned 3 groups (P<0.05). Organ damages as shown in burn control group [cTnI (4.86+0.29) μg/L, TBA (83.6+18.2) μmol/L, β2-MG (2.75±0.19) mg/L, DAO (1.45±0.09)×103 U/L] were more serious than those in sham control group [ cTnI (1.73±0.09) μg/L, TBA (24.5± 2.4)μmoL/L, β2-MG (1.15±0.18) mg/L, DAO (0.87±0.13)×103U/L] , and less serious than those in scald + propranolol group [cTnl 5.95±0.42 μg/L, TBA 125.8±21.3 μmol/L, β2-MG 3.25 ±0.17 mg/L, DAO (1.83±0.13)×103 U/L ] ( P <0.05 ). Conclusions Propranolol can aggravate injury to the liver, kidney and intestine at early stage in rat with severe burn, suggesting that "shock heart" may be one of initial factors in lowering blood flow to the organs, thus inducing injury to them.     

IgA肾病患者外周血γδT细胞分泌TGF-β1的功能

目的：研究IgA肾病患者外周血γδT细胞分泌TGF-β1的功能。方法：用流式细胞仪分析外周血γδT细胞比率并检测血清IgA的水平，以抗TCRγδ单克隆抗体（mAb）固相法，体外选择性地扩增γδT细胞住体外培养的条件下，以抗CD3抗体刺激48h后．用ELISA法测定培养体系中TGF-β1的水平。结果：IgA肾病患暂外周血γδT细胞的比例升高，并与血清IgA的水平呈正相关。用抗TCRγδmAb固相法能获得高纯度的γδT细胞。经抗CD3抗体刺激的IgA肾病患者的γδT分泌TGF-β1的量比正常对照组明显升高。结论：IgA肾病患者的γδT细胞可通过大量分泌TGF-β1参与该病的发生、发展。     

７ 羟胆固醇对神经细胞氧化损伤作用的研究

摘要：目的　探讨２７ 羟胆固醇（２７ ｈｙｄｒｏｘｙｃｈｏｌｅｓｔｅｒｏｌ,２７ ＯＨＣ）对神经细胞的氧化损伤作用。方法　以
神经细胞（ＳＨ ＳＹ５Ｙ 细胞系）为研究对象,共分４组：对照组、２７ ＯＨＣ５μｍｏｌ／Ｌ 组、２７ ＯＨＣ１０μｍｏｌ／
Ｌ 组、２７ ＯＨＣ２０μｍｏｌ／Ｌ 组。用化学荧光法检测神经细胞活性氧（ＲＯＳ） 水平;用ＷＳＴ １法检测神经细
胞中超氧化物歧化酶（ＳＯＤ）活力;用微量酶标法检测还原型谷胱甘肽（ＧＳＨ）水平;用ＥＬＩＳＡ 法检测８
羟基脱氧鸟苷（８ ＯＨｄＧ） 水平。实验结果采用ＳＰＳＳ１８．０ 进行数据录入和单因素方差分析（ＡＮＯＶＡ）。
结果　随着２７ ＯＨＣ 浓度升高,各组细胞ＲＯＳ水平逐渐升高,２０μｍｏｌ／Ｌ 组（４０８５．３３±１０５９．２３） 较对
照组（２５３８．６７±２０８．６９）和５μｍｏｌ／Ｌ 组（２５９７．００±３０１．０４） 差异均有统计学意义（犘＜０．０５）; 各组
ＳＯＤ 活力（１１．８６±０．４３,１０．６７±０．０３,１０．１５±０．１４ Ｕ／ｍｇ蛋白） 较对照组（１４．９４±０．３５ Ｕ／ｍｇ蛋白）
逐渐降低,差异均有统计学意义（犘＜０．０５）,１０μｍｏｌ／Ｌ 组和２０μｍｏｌ／Ｌ 组较５μｍｏｌ／Ｌ 组差异有统计学意
义（犘＜０．０５）;各组ＧＳＨ 水平较对照组（２２７５．２５±１２３．０１μｍｏｌ／ｇ 蛋白） 逐渐降低（犘＜０．０５）,１０
μｍｏｌ／Ｌ 组（１８６４．２３±１４５．８９μｍｏｌ／ｇ蛋白）、２０μｍｏｌ／Ｌ 组（１７５６．４４±１８１．８２μｍｏｌ／ｇ蛋白）较对照组和
５μｍｏｌ／Ｌ 组（２１５８．５９±２４１．３３μｍｏｌ／ｇ 蛋白） 差异均有统计学意义（犘＜０．０５）; 各组８ ＯＨｄＧ 浓度
（２．８９±０．３２,４．０２±０．１１,４．１６±０．０９μｇ／Ｌ）较对照组（２．３０±０．１４μｇ／Ｌ）均显著升高（犘＜０．０５）,１０
μｍｏｌ／Ｌ 组和２０μｍｏｌ／Ｌ 组较５μｍｏｌ／Ｌ 组差异有统计学意义（犘＜０．０５）。结论　２７ 羟胆固醇对神经细胞具
有氧化损伤作用,体现在增加神经细胞ＲＯＳ、８ ＯＨｄＧ 水平,降低神经细胞ＳＯＤ 活力和ＧＳＨ 水平。
关键词：２７ 羟胆固醇;神经细胞;氧化损伤
中图分类号：Ｒ１５１．２　　文献标识码：Ａ　　文章编号：１００９ ６６３９ （２０１４）０６ ０５７６ ０６     

肾上腺素对THP-1源巨噬细胞SR-AI与ABCA1表达的影响

目的 探讨肾上腺素对THP-1源巨噬细胞三磷酸腺苷结合盒转运体A1（ABCA1）与清道夫受体AI（SR-AI）mRNA表达的影响。方法 不同浓度肾上腺素（1pmol／L～1μmol／L）处理THP-1源巨噬细胞24h，运用逆转录多聚酶链反应检测其ABCAl与SR-AI mRNA表迭。结果 1pmol／L～10nmol／L的肾上腺素对受试基因mRNA的表达与相应对照组比较其差异无统计学意义（P〉0．05）。而100nmol／L及1μmol／L的肾上腺素上调SR-AI mRNA水平和下调ABCA1 mRNA水平，与各自对照组比较其差异有统计学意义（P〈0．05）。结论 肾上腺素上调THP-1源性巨噬细胞SR-AI mRNA水平，下调其ABCA1 mRNA水平。     

富含黄酮类蔬菜和果汁对机体抗氧化功能影响

活性氧所引起的氧化损伤与多种疾病的发生密切相关^〔1-2〕。从膳食中直接获得抗氧化物质或具有激活体内抗氧化防御体系功能的物质被认为是预防氧化损伤相关疾病的可行方法^〔3-4〕。