NLR及PCT联合MCTSI对重症胰腺炎感染性坏死早期预测价值

目的 探讨中性粒细胞与淋巴细胞比值(NLR)、降钙素原(PCT)与改良CT严重指数评分(MCTSI)联用对重症急性胰腺炎(SAP)继发感染性胰腺坏死(IPN)的早期预测价值.方法 分析324例SAP病人的临床资料,根据是否发生IPN,分为感染组(144例)和非感染组(180例),比较两组NLR、PCT及MCTSI差异....     

北京市崇文区社区卫生服务机构健康教育开展现状

目的了解北京市崇文区居民对社区卫生服务机构的健康教育需求,为有针对性的开展健康教育活动提供依据。方法采用随机抽样的方法,对崇文区参加健康体检的常住居民进行社区卫生服务机构健康教育现状及认可程度调查。结果居民对社区卫生服务健康教育的满意率为22.67%。在社区卫生服务机构优劣势的调查中,90.25%的调查对象认为社区卫生服务机构开展的健康教育知识具有科学性和针对性,但92.54%的人觉得大医院在健康教育方面更具有权威性。居民对高血压、糖尿病、冠心病等日常保健知识需求最高,为90.63%,其认可率也最高,为96.56%;对儿童与青少年视力保护保健知识的需求率最低,为23.90%,其认可率也最低,为1.23%。结论社区健康教育机构能够提供经济、快捷、质优、方便的健康教育,但也存在着卫生服务水平不高的现状,应在加大社区健康教育覆盖面的同时,不断提高从业人员的卫生服务能力。     

时间序列分析在北京市东城区艾滋病病毒感染者和艾滋病患者发病率预测中的应用

目的 探讨应用时间序列差分自回归移动平均模型（ARIMA）预测北京市东城区艾滋病病毒感染者/艾滋病患者（HIV/AIDS）发病率的可行性。方法 应用SAS 9.3软件对北京市东城区2005-2014年HIV/AIDS月发病率构建ARIMA模型,用得到的模型对2015年HIV/AIDS月发病率进行预测,评价模型的拟合和预测效果。结果 ARIMA（0,1,1）（0,1,1）12模型拟合效果较好,但仍有优化的空间,月发病率的实际值均在预测值的95%可信区间内,预测值与实际值拟合趋势一致。结论 ARIMA模型可以对北京市东城区HIV/AIDS月发病率进行预测,为艾滋病疫情预测提供依据。     

末端驱动型下肢机器人对脑卒中患者步态的影响

目的:探讨末端驱动型下肢康复机器人训练对脑卒中患者下肢步行能力、下肢运动功能、步态及平衡功能的影响。方法:将2018年8月至2019年8月在空军军医大学第一附属医院住院治疗的、符合纳入标准的39例脑卒中患者,按照随机数字表法将其分为训练组(21例)和对照组(18例)。2组患者均接受常规康复治疗,包括偏瘫肢体综合训练、中...     

中医诊疗仪器研究进展

中医学经历了几千年的发展,无论从诊断和治疗方面都积累了丰富的经验,是一个伟大的知识宝库.但是与现代医学相比,虽然中医学认识理念先进,但是其利用现代技术和手段相对滞后,在一定程度上阻碍了中医药的现代化和国际化.随着现代科学技术的进步,传统的中医药理论与现代科学技术的结合促进了中医工程的发展,无论是诊断还是诊疗都取得了突破性进展,尤其是现代化的中医诊疗仪器.目前,中医诊断仪器以舌脉象仪研究为主,逐渐向四诊综合分析平台发展;中医治疗仪器以电针仪、针麻仪、激光治疗仪、经络导平仪及磁疗仪器为主.将目前研究的中医诊疗仪器分为中医诊断仪器、中医治疗仪器与中药仪器3类,并对其研究现状、存在问题与发展趋势进行综述.     

腺病毒介导的白细胞介素10基因转移对胰岛β细胞凋亡及胰岛素释放的影响

Objective To evaluate the efficacy of adenovirus vector-mediated murine interleukin-10 (mIL-10) gene transfer to rat beta cell-RINm5F cells in vitro and to explore the potential value of gene therapy in type 1 diabetes mellitus. Methods The recombinant adenovirus vector Ad-mIL-10 was constructed and transfected into RINm5F cells (Ad-mIL-10 group). Untransfected RINm5F cells and Ad-eGFP-transfected cells were used as controls. The expression of mIL-10 was examined by RT-PCR and Western blot The levels of IL-10 in supernatant were measured by enzyme-linked immunosorbent assay (ELISA). For a determination of insulin release, the cells were cultured with high glucose ( 16. 7mmol/L) for a 1 -hour co-incubation. Then radioimmunoassay was used to detect the level of insulin in supernatant After induction of IL-1β, the levels of nitric oxide (NO) and nitric oxide synthase ( NOS) were measured, the apoptosis of transfected cells was detected by Hoechst 33258 staining and Fas expression by flow cytometry. Results Both mRNA and protein of Ad-mIL-10 were successfully expressed in RINm5F cells. Expression of mIL-10 gene resulted in significant increases in insulin secretion in response to high glucose. Compared with uninfected control and Ad-eGFP infected group, Ad-mIL-10 infected group had decreased levels of NO and NOS induced by IL-lβ [ NO level ( nmol/106 cells): 52. 9 ± 3. 2 vs 227. 3 ± 26. 4, 235. 1 ±28.6, both P＜0.05; NOS level (U/106 cells): 9. 3 ±1.2 vs 29. 8 ±2.5, 30.5 ±2.8, both P ＜ 0. 05]. Furthermore, Ad-mIL-10 gene transfer led to a profound reduction of Fas-expressing islet cells under the induction of IL-1β. Fas-expressing islet cells in Ad-mIL-10 group were significantly lower than those in uninfected group and Ad-eGFP-transfected group (24. 6% ± 1.0% vs 33. 3% ± 5. 1% , 32. 6% ±1.1%) (P＜0.05).The apoptotic rates of Ad-mIL-10 group were lower in comparison with the other two groups (9.4%±1.1% vs 19.2%±2.2%,20.6%±2.3%,P＜0.05).Conclusions IL-10 gene transfer to islet beta cells may be beneficial in maintaining beta cells function,protecting islet cells from IL-1β-mediated apoptosis and promoting islet cells survival.It is a potential therapy for type 1 diabetes mellitus.     

中医药治疗特发性血小板减少性紫癜的研究进展

<正>特发性血小板减少性紫癜(Idiopathic thrombocytopenic purpura,ITP)是因血小板免疫性破坏,导致外周血中血小板减少的出血性疾病,也叫特发性自体免疫性血小板减少性紫癜,以广泛皮肤黏膜及内脏出