《中国药典》2005年版药材饮片中的若干问题及改进建议(待续)

《中国药典》2005年版(简称新版药典)的颁布实施,是我国医药卫生界的大事。同2000年版相比,新版药典一部新增品种154种,修订品种453种,较好地反映了我国当前中药的发展水平,是有关领导部门和科技人员共同努力的结果,是对中医药事业的可贵贡献。药材饮片是中药治病的物质基础,其“功能与主治”既是直接指导临床用药的依据,又是论述成方制剂“功能与主治”的重要依据,而药材的“功能与主治”又同其“性味与归经”“用法与用量”“....     

帕罗西汀片在健康人体的药代动力学

目的 研究帕罗西汀片在健康人体内的药代动力学.方法 20名健康志愿者单剂量121服40 mg帕罗西汀片后,用高效液相色谱法测定血浆中帕罗西汀浓度,并用3P97软件统计处理.结果 帕罗西汀片药-时曲线经拟合呈二室模型,其Cmax、Tmax 、AUC0.120、AUC0.inf分别为(64.74±18.43)ng/ml、(5.64±1.84)h、(20.03±5.33)h、(976.47±309.49)ng·h/ml和(1086.75±376.54)ng·h/ml.结论 帕罗西汀片在人体内的药代动力学呈二房室开放模型.     

建立开放式口腔实训中心的探索

口腔医学是一门实践科学,在每个口腔专业的教学中,理论教学和实践教学共同组成了其完整的教学体系.如何提高学生的动手操作能力,提高实践教学的质量是口腔医学教学需要解决的问题.而将口腔实训中心开放,给学生更多的操作练习机会,是解决这个问题的一条很好途径.     

铁调节蛋白2基因多态性与阿尔茨海默病及血管性痴呆的相关分析

Objective To evaluate the association of 2616c/T polymorphism in iron regulatory protein 2(IRP2)gene with Alzheimer disease(AD)and Vascular dementia(VD).Methods In this study,281 patients with AD,60 with VD,and 285 normal aged were recruited.The 2616C/T polymorphism in IRP2 gene was analyzed by using polymerase chain reaction-restriction fragment length polymorphism.And the cognitive function was assessed with the Mini-Mental State Examination(MMSE).Results (1)No significant difieFences were demonstrated in IRP2 genotype or allele frequencies between AD patients and controls(χ2=2.46,P=0.292;χ2=2.17,P=0.141 respectively).However,when AD patients were stratified by disease severity.the frequency of T allele carriers in the moderate to severe AD patients was 78.0%,significantly higher than that in controls(69.8%;χ2=4.106,P＜0.05).Logistic regression analysis demonstrated that the age-,sex-and ApoE-adiusted OR of modcrate to severe AD patient with T allele was 1.62(95% CI=1.03-2.54).The frequency of T allele carriers or T allele in VD patients was higher than that of controls,but the difference was not statistically significant(P＞0.05).(2)The frequency of tit genotype or T allele in the moderate to severe AD patients was significantly higher than that in mild AD patients(25.8%vs.12.5%,χ2=5.477,P＜0.05;51.9%vs.40.3%,χ2=5.803,P＜0.05 respectively).(3)MMSE scores of the AD patients with TT genotype was significantly lower than ones with CC or CT genotype(P=0.028;P=0.014 respectively).Conclusion The 2616C/T polymorphism in the IRP2 gene is possibly associated with moderate to severe AD.but not associated with VD.And the TT genotype may be a risk factor for cognitive impairment of patients with AD in Chinese Han.     

经额眶入路切除眶尖肿瘤11例疗效分析

目的 探讨采用经额眶入路摘除眶尖肿瘤的疗效。方法 11例眶尖肿瘤，手术先由神经外科作经额部的4边形额肌骨瓣，然后沿前颅窝底取下眶顶骨板、切开骨膜入眶。随后由眼科医生进行眶内肿瘤摘除的操作，再由神经外科医生继续处理有颅眶沟通者的颅内部分并关闭切口。结果 11例中有9例显微镜下完整摘除肿瘤，2例部分摘除。术后视力无下降，术前9例视力下降者，术后7例有提高。眼球突出度平均降低1．5mm；眼球轻度下陷1例；术后发热1例。无1例发生与开颅有关的并发症。结论 眼科与神经外科联台行经额眶入路眶尖肿瘤摘除术，可以满意地解决眶尖肿瘤难以暴露的问题，绝大多数良性肿瘤可完整摘除甚至是在显微镜下摘除，有利于减少手术并发症的发生，疗效满意。     

虚拟仿真系统在口腔实验教学中的应用

口腔实验教学中采用计算机虚拟仿真技术,通过虚拟仿真操作进行训练,运用实时感知的三维立体界面,允许学生对其交互仿真操作,调动学生学习的积极性和主动性,让学生反复操练,提高诊疗操作能力。优化学生实习前培训,加强学生理论知识学习和实践操作的有机结合。本文将对虚拟仿真系统在口腔实验教学中的应用现状作一分析。     

铁调节蛋白2基因多态性与阿尔茨海默病及血管性痴呆的相关分析

Objective To evaluate the association of 2616c/T polymorphism in iron regulatory protein 2(IRP2)gene with Alzheimer disease(AD)and Vascular dementia(VD).Methods In this study,281 patients with AD,60 with VD,and 285 normal aged were recruited.The 2616C/T polymorphism in IRP2 gene was analyzed by using polymerase chain reaction-restriction fragment length polymorphism.And the cognitive function was assessed with the Mini-Mental State Examination(MMSE).Results (1)No significant difieFences were demonstrated in IRP2 genotype or allele frequencies between AD patients and controls(χ2=2.46,P=0.292;χ2=2.17,P=0.141 respectively).However,when AD patients were stratified by disease severity.the frequency of T allele carriers in the moderate to severe AD patients was 78.0%,significantly higher than that in controls(69.8%;χ2=4.106,P＜0.05).Logistic regression analysis demonstrated that the age-,sex-and ApoE-adiusted OR of modcrate to severe AD patient with T allele was 1.62(95% CI=1.03-2.54).The frequency of T allele carriers or T allele in VD patients was higher than that of controls,but the difference was not statistically significant(P＞0.05).(2)The frequency of tit genotype or T allele in the moderate to severe AD patients was significantly higher than that in mild AD patients(25.8%vs.12.5%,χ2=5.477,P＜0.05;51.9%vs.40.3%,χ2=5.803,P＜0.05 respectively).(3)MMSE scores of the AD patients with TT genotype was significantly lower than ones with CC or CT genotype(P=0.028;P=0.014 respectively).Conclusion The 2616C/T polymorphism in the IRP2 gene is possibly associated with moderate to severe AD.but not associated with VD.And the TT genotype may be a risk factor for cognitive impairment of patients with AD in Chinese Han.     

儿童多发性硬化视力障碍分析

多发性硬化（MS）是一种慢性、复发性、多病灶性中枢神经系统白质脱髓鞘疾病.但除脑、脊髓以外,视神经亦常受累,以至在相当长时间内以视力损害为首发的唯一症状而就诊于眼科.儿童病例以往少见,近几年有增加的趋势.现对我院确诊的11例儿童MS患者进行分析,以期提高对本病的重视.     

中药饮片用量的探讨（上）

以《中国药典》收载的饮片用量，同全国擅用该饮片的名老中医经验以及重要中药文献的相关记载进行比较，大量实例提示，药典中诸多饮片用量，在辨证与用量的关系、用量更新及用量大小等方面，均存在欠妥，不利于指导临床用药，亟待改进。为此，笔者在反复思考“中医不传之秘在于量”之后，提出应当高度重视饮片用量研究和应用研究的3点建议。     

反相高效液相色谱法测定人血浆奋乃静浓度

目的 建立反相高效液相色谱法测定人血浆中奋乃静的浓度。方法 以钻石C₁₈反相柱(150 mm×4.6 mm, 5 μm)为色谱柱,流动相为0.03 mol·L-1醋酸铵-甲醇（23：77）；流速：1.0 mL·min^-1；柱温：40 ℃;检测波长: 254 nm。以醋酸乙酯与二氯甲烷(80：20)为提取剂。结果 奋乃静的高、中、低（1 000.0, 400.0,10.0 μg·L^-1）3种浓度平均回收率分别为104.28%,97.60%,98.40%,日内、日间差RSD均<7%（n=5）;分析方法的检测限为5.0 μg·L^-1;线性范围为10.0～1 000.0 μg·L^-1。结论 该方法灵敏、准确、简单、快速,可用于临床血药浓度监测和药动学研究.