Actions of four organic acids in radix isatidis on endotoxin-neutralization investigated by kinetic turbidimetric assay

OBJECTIVE:To investigate anti-endotoxin action of four OAs reacted with endotoxin by the LAL assay with KTA.METHODS:Using a incubating kinetic tube reader and kinetic turbidimetric assay(KTA),the concentration-response time curve of endotoxin reacted with limulus amebocyte lysate(LAL) at 37℃ were obtained and the action of four organic acids(OAs) on it were investigated.The four OAs were benzoic acid,salicylic acid,syringic acid and 2-amino-benzoic acid from Radix isatidis.Meanwhile,the temperature variation caused by endotoxin with the four OAs was studied by the rabbit pyrogen test(RPT).RESULTS:It was showed that a low concentration(1 mg/mL) of the four OAs had a little effect of anti-endotoxin,and when the concentrations of the four OAs were 30 mg/mL,the endotoxin was neutralized completely.The relationships between the concentrations of endotoxin and the OAs were all linear with correlation coefficients of greater than 0.9995,indicating that the four OAs all had strong anti-endotoxin action,while syringic acid had the strongest action among the four OAs with IC50 of 12.84 mg/mL.CONCLUSION:The investigations of KTA agreed well with the results obtained by means of RPT.     

农村老年性高血压206例临床分析

总结和探讨农村老年性高血压病机制和预防措施,为老年性高血压,流行病学调查提供资料和借鉴。 1临床资料 现总结我院2003～2007年现治老年性高血压206例与非老年性高血压144例（非老年组）对比临床资料报告如下：     

CD437诱导HepG2细胞凋亡及机制的初步研究

Objective To investigate the effect of CD437 on apoptosis of human hepatoblastoma cell line HepG2 and the possible role of CD437 on mitochondrial membrane potentials (MMP). Meth-ods The effect of CD437 on the proliferation of HepG2 cells was evaluated with MTT assay. Flow cytometry was performed after HepG2 cells were labeled with Annexin V-FITC/PI double fluores-cence. Mitochondrial membrane stained with Rhodamine 123 probes was detected under fluorescent microscope and the changes of MMP were analyzed with flow cytometry. Results After incubated with 1μmol/L of CD437 for 24 hours, both the earlier and later apoptotic rates of HepG2 cells in the exper-imental group increased significantly compared to those in the control group. The fluorescent intensity detected with flow cytometry in the experimental group was significantly lower than that in the control group (5. 34 ± 0. 12 vs 4. 40 ± 0. 09, P<0. 05). The left peak-shift and decreased MMP were noted in the experimental group. Under the fluorescent microscope, fluorescence of HepG2 stained with Rho-damine 123 probes disappeared after incubated with CD437. Conclusions CD437 can significantly in-hibit the growth of HepG2 and induce the apoptosis by decreasing MMP.     

蛔虫症和胆石形成关系的初步探讨

胆石形成的机制,目前尚未充分了解。有关胆石形成与蛔虫感染的关系国内早有报导。但各地报导的百分率不一,相差悬殊,究其原因除了可能与蛔虫感染流行地区有关外,亦可能与检查方法有关。我们搜集50例患者之胆石标本共130块(指直径在0.5cm 以上者),计胆囊结石25块,总胆管结石75块,肝管结石26块、大便排石4块;分别用显微镜解剖法、石腊切片法、化学溶解法或涂片法对比检查胆石核心部位,现将我们的检查方法、检查结果及体会予以介绍。     

肝母细胞瘤治疗新进展

肝母细胞瘤是最常见的肝脏原发性恶性肿瘤,多见于婴幼儿,尤以出生后1～2年发病最多见,东南亚地区的发病率高于欧洲及北美地区,其详细发病机制尚未完全明了。近年来对患儿进行综合治疗,明显提高了生存率。现从外科手术治疗、增加手术切除机会的手段、药物治疗三方面将近年来的治疗进展做一综述。     

RHBDF2基因过表达慢病毒载体的构建和稳定表达RHBDF2细胞系的建立

目的：构建RHBDF2基因过表达慢病毒载体,建立稳定表达RHBDF2的EA.hy926细胞,为RHBDF2基因过表达慢病毒载体的构建和稳定表达RHBDF2细胞的建立提供依据。方法：根据NCBI提供的RHBDF2基因序列,设计并合成引物,PCR扩增RHBDF2基因后通过Gateway克隆技术将目的基因克隆至入门载体,再亚克隆至慢病毒载体pLV[Exp]-EGFP,构建重组慢病毒质粒pLV[Exp]-EGFP-RHBDF2;将慢病毒表达载体质粒pLV[Exp]-EGFP和重组慢病毒质粒pLV[Exp]-EGFP-RHBDF2分别与慢病毒辅助包装质粒共转染HEK293T细胞,包装慢病毒并测定慢病毒滴度;将感染pLV[Exp]-EGFP-control的EA.hy926细胞作为对照组,感染pLV[Exp]-EGFP-RHBDF2的EA.hy926细胞作为实验组,利用嘌呤霉素筛选出稳定表达RHBDF2的EA.hy926细胞;荧光定量PCR （qPCR）和Western blotting法检测对照组和实验组EA.hy926细胞中RHBDF2 mRNA和蛋白表达水平。结果：酶切电泳和测序检测,实验组过表达慢病毒载体的基因序列与设计合成的序列完全一致;对照组包装的慢病毒滴度为1×10⁸ TU·mL^-1,实验组慢病毒的滴度为3×10⁸ TU·mL^-1;荧光显微镜下慢病毒成功感染EA.hy926细胞,感染率达95%以上;qPCR检测,实验组EA.hy926细胞中RHBDF2 mRNA表达水平较对照组明显升高（P<0.01）;Western blotting法检测,实验组EA.hy926细胞中RHBDF2蛋白表达水平较对照组明显升高（P<0.05）。结论：成功构建RHBDF2过表达慢病毒载体,利用pLV[Exp]-EGFP-RHBDF2慢病毒建立了稳定上调RHBDF2表达的EA.hy926细胞系。     

云南某高校大一学生手机辐射认知现状

目的了解大一学生对手机辐射相关知识的认知程度,为对大学生开展手机辐射相关知识的宣传教育提供参考依据。方法采用手机辐射相关知识问卷,对某高校470名大一学生对手机辐射相关知识了解情况进行调查。结果大一学生对手机产生辐射的知晓率为90.7%,但对具体的手机辐射相关知识及辐射防护相关知识知晓率相对较低。除手机产生辐射、手机辐射危害眼睛及运动可减少辐射危害(P<0.05),外其他各项男、女生之间差异均无统计学意义(P值均>0.05)。来自城市的学生对手机辐射及防护知识知晓率大多数高于来自农村的学生。结论学校有必要加强学生对手机辐射知识和防护措施的健康教育,以利于学生的身体健康。     

嘉禾县2007～2010年乡村医疗机构消毒效果监测分析

目的 了解嘉禾县乡村医疗机构的消毒状况,为科学指导基层医疗机构消毒工作、控制医源性感染提供科学依据.方法 按《消毒技术规范》、《医院消毒卫生标准》和《消毒与灭菌效果的评价方法与标准》方法,对2007～2010年乡村两级医疗卫生单位消毒效果进行检测.结果 2007～2010年各年度消毒监测合格率分别为76.8％,81.7％,85.6％,89.1％,呈逐年上升趋势,各年度之间差异有统计学意义(P＜0.05).乡村两级医疗机构平均合格率分别为91.3％,80.8％,二者之间差异有统计学意义.各类样品中使用中消毒液合格率较高,物体表面合格率较低.结论 嘉禾县2007 ～2010年乡村医疗机构消毒效果逐年提高,但消毒监测工作仍需加强,需继续加强对基层医务人员消毒知识培训和技术指导.     

microRNA-137基因多态性与缺血性卒中后抑郁的相关性

目的分析microRNA-137基因多态性与缺血性卒中后抑郁的相关性。方法2017年1月至2019年1月,SNaPshot检测缺血性卒中后抑郁患者250例(患者组)和健康体检者250例(对照组)microRNA-137基因rs1625579位点的基因型,逆转录荧光定量聚合酶链反应检测外周血单个核细胞microRNA-137相对表达量,并分析其与rs1625579位点不同基因型的关系。结果患者组rs1625579位点等位基因T高于对照组(OR=2.033,95%CI 1.255~3.294,P=0.003),基因型TT高于对照组(OR=2.139,95%CI 1.272~3.595,P=0.004);患者组microRNA-137相对表达量显著低于对照组(t=28.230,P<0.001);对照组TT基因型的microRNA-137相对表达量显著低于GT+GG基因型(t=3.764,P<0.001)。结论microRNA-137基因rs1625579位点TT基因型可能通过下调microRNA-137的表达,参与缺血性卒中后抑郁的发病。     

miRNA-9参与脑卒中调控的研究进展

脑卒中是一种常见的脑血管疾病,也是威胁人类健康最严重的疾病之一。目前溶栓及机械介入取栓等治疗方案已广泛运用于临床医疗,但由于狭窄的时间窗限制以及可能发生的并发症等难题,需要进一步探究脑卒中发生、发展过程中的分子机制,期望能提供更有效的预防策略和更安全的治疗方案。miRNA是一类能够特异性结合mRNA的低分子RNA,研究表明,miRNA-9是一种与神经功能相关的miRNA,参与调控了神经细胞的增殖、凋亡和细胞周期等生物学过程,因此它可能在脑卒中的发生、发展过程中起重要作用。本文就miRNA-9在脑卒中发病过程中参与调控的分子机制做一综述。