pSVPoMcat微基因修饰雪旺氏细胞移植对损伤脊髓前角神经元的保护作用研究

目的：探讨pSVPoMcat微基因修饰雪旺氏细胞移植对损伤脊髓前角神经元的保护作用。方法：采用大鼠脊髓半横切损伤模型，将实验动物分为3组：pSVPoMcat微基因修饰雪旺细胞移植组（A组），单纯雪旺氏细胞移植组（B组），损伤对照组（C组）。对脊髓切片行Nissl染色，酸性磷酸酶（ACP）组织化学染色及原位末端标记（TUNEL）法染色，并用联合行为记分（CBS）观察大鼠神经功能恢复情况。结果：A、B、C三组前角神经元存活率呈显著性差异（A＞B＞C，P＜0．01）；A组ACP变化幅度明显降低（A＜B＜C）；细胞凋亡率为C＞B＞A。大鼠神经功能恢复也出现了相同的变化趋势。结论：pSVPoMcat微基因修饰雪旺氏细胞移植对脊髓损伤前角神经元有保护作用并促进大鼠损伤脊髓功能的恢复。     

人脑胶质瘤中增殖细胞核抗原、p53、p21ras及EGFR蛋白的表达

目的:探讨人脑胶质瘤中多种基因蛋白的异常表达及意义。方法:应用免疫组化技术检测102例手术切除人脑胶质瘤组织标本中增殖细胞核抗原(PCNA)及p53,p21ras及EGFR蛋白的表达。胶质瘤组织PCNA标记指数(PCNALI)为556±155,p53,p21ras及EGFR蛋白的表达率分别为586%,523%,42%,均显著高于正常脑组织。PCNALI及EGFR蛋白表达与胶质瘤恶性程度相关(P<001),p53,p21ras及EGFR蛋白的表达与胶质瘤恶性程度无关(P>005)。胶质瘤中PCNALI与EGFR蛋白表达无明显相关(P>005),而与p53和p21ras表达相关(P<005)。结论:p53,p21ras和EGFR异常表达与胶质瘤的发生发展相关,PCNALI是评估增殖活性的良好指标。     

三七总皂甙对家兔脑损伤后脑组织中 丙二醛含量变化的影响

目的:研究三七总皂甙(PNS)对减轻颅脑创伤后脑组织损伤机制。方法:改良八木国夫法检测家兔脑损伤后24 h脑组织中的丙二醛(MDA)含量、脑组织含水量及脑组织超微结构改变,观察PNS对上述指标的影响。结果: 脑损伤后24 h脑损伤+PNS组及对照组脑组织中MDA含量分别为(81.43±9.72) nmol/g湿脑,(76.87±8.31) nmol/g湿脑,明显低于脑损伤组MDA含量(106.52±17.68) nmol/g湿脑,(P＜0.01);脑损伤+PNS组及对照组脑组织中水含量分别为78.97%±0.91%,78.35%±0.89%,明显低于脑损伤组80.74±0.98(P＜0.01)。损伤脑组织中MDA含量与伤后脑水肿程度呈正相关(P＜0.01),同时脑损伤组出现明显的脑组织超微病理改变。 结论:PNS能够降低颅脑损伤后自由基的产生,减轻脑水肿和脑组织病理损害,对颅脑损伤具有一定的脑保护作用。     

颅脑火器伤动物模型

本文对近年来颅脑火器伤动物模型的制作方法及模型的病理、病生、代谢等变化的观测方法和结果进行了回顾,对各项方法的优缺点进行了客观的分析,提出今后应对创伤比例及继发性损伤时的综合代谢变化予以重视。     

移植pSVPoMcat微基因修饰雪旺细胞在鼠脊髓内长期存活及其基因表达

目的：观察人Po-5,flanking介导的21．5ku髓鞘碱性蛋白（myelin basic protiein,MBP)微基因（暂命名为pSVPoMcat)修饰雪旺细胞（SC）在鼠脊髓内存活及其基因表达。方法：120只大鼠分为3组,A组为pSVPoMcat微基因修饰SC移植组;B组为高纯化SC移植组;C组为脊髓损伤（SCI）＿对照组,伤后各组分别于2,4,8,12周（每组每次10只）,取移植区脊髓切片,进行S－100蛋白,MBP免疫细胞化学染色及地高辛(DIG)标记的hMBPF2 cDNA探针的原位杂交（ISH）测定。结果：伤后2,4,8,12周,A组S－100,MBP染色细胞和ISH细胞差异无统计学意义（P＜0．05）,其中ISH阳性细胞可见髓鞘形成,B组的S－100染色细胞逐步递减,差异有非常显著性意义（P＜0．01）,未发现MBP染色细胞以及ISH细胞,C组未发现任何阳性细胞,结论：pSVPoMcat微基因修饰SC在鼠损伤脊髓内能长期存活并表达外源基因,且有助于受伤脊髓的髓鞘形成。     

Effect of Microgene pSVPoMcat Implantation to Modify Genetically Schwann Cells on Central Conduction Function after Spinal Cord Injury

We have tested cortical somatasensory evoked potentials (CSEP)after spinal cord injury treated by pSVPoMcat modified Schwann cells implantation and the change of combined behavior score introduced by Gale et al.to explain its effect on central condution after experimental SCI.1 Materials and Methods1.1 SCI model and implantation process Adult SD rats( weighing 200－230g,with no gender regarded,provided by the animal Center of the West China University of medical)were made hemi－transe…     

微基因修饰雪旺氏细胞移植对脊髓损伤中枢传导功能的作用

Objective To approach the effect of microgene pSVPoMcat to modify genetically Schwann cells (SC) on central conduction functionafter spinal cord injury (SCI) . Method Experimental animals were divided into three groups: the group of microgene pSVPoMcat implanted to genetically modify SC (group A), SC implanted group (group B), and the control group (group C). The cortical somatasensory evoked potentials (CESP) and combined behavioral score (CBS) were continuous monitored from operation second week to twelfth week Result The result showed that the peak latency and peak amplitudes of group A, B have a recovery tendency and it was constant with CBS. Conclusion We concluded that the microgene pSVPoMcat to modify genetically Sc may play a promotion role in recovery of central conduction function after SCI.     

移植pSVPoMcat微基因修饰雪旺氏细胞对脊髓损伤早期作用的实验研究

目的 探讨移植pSVPoMcat微基因修饰雪旺细胞(SC)对损伤早期脊髓组织的作用。方法 切割法造成脊髓半横断损伤(T8平面)模型。术后将实验动物分为pSVPoMcat微基因修饰SC组(A组),SC移植组(B组),损伤对照组(C组)和正常对照组(D)。术后8h,取脊髓伤段标本,用地高辛(DIG)标记的C-fos探针行原位杂交,并分别用干湿法,原子吸收光谱法测量水、钙含量和比色法测量雨二醛(MDA)含量。结果 1.脊髓损伤(SCI)后,伤段脊髓组织C-fos基因在各试验中表达顺序为,C>B>A>D,P<0.05。2.损伤脊髓段组织钙含量,MDA含量明显增多,组织水肿严重,在各实验组中,水、钙及MDA含量为C>B>A>D,结论 移植pSVPoMcat微基因修饰SC对损伤脊髓早期有明显的保护作用,其保护作用与抑制C-fos基因表达,稳定钙离子水平及降低MDA含量有关。     

侧脑室肿瘤的显微手术治疗

目的 进一步提高侧脑室肿瘤手术效果。方法 27例CT或MR Ⅰ确诊的侧脑室肿瘤,采用显微外科手术治疗,手术入路包括额中回、顶上小叶、颞中回和三角区。结果 肿瘤全切除16例.次全切除7例,部分切除4例,手术重残1例,死亡1例。12例经2～7年随访,肿瘤无复发。结论 只有根据侧脑室肿瘤大小和部位选择合适入路,采用显微手术切除,才能避免不必要的神经功能缺失和达到肿瘤的充分切除。     

pSVPoMcat modifying Schwann cell to protect injured spinal neurons in rats

OBJECTIVE: To investigate the protective effect of pSVPoMcat (myelin basic protein microgene) modifying Schwann cell on injured spinal neurons. METHODS: A model of rat spinal cord injured by hemisection was used. One hundred and twenty healthy SD rats of both sexes weighing 250-300 g were divided into three groups: Group A (n=40, treated with implantation of pSVPoMcat modifying Schwann cell), Group B (n= 40, treated with implantation of Schwann cell only) and Group C (n=400, treated with sham operation as the control). One week after operation the rat functional recovery was observed dynamically by using combined behavioral score (CBS) and cortical somatasensory evoked potentials, the spinal cord sections were stained by Nissl, acid phosphatase enzyme histochemistry and cell apoptosis was examined by methye green, terminal deoxynucleotidyl and the dUTP Nick end labeling technique. Quantitative analysis was done by computer image analysis system. RESULTS: In Group A the injured neurons recovered well morphologically. The imaging analysis showed a result of Group A相似文献