全文获取类型
收费全文 | 1778篇 |
免费 | 142篇 |
国内免费 | 11篇 |
专业分类
耳鼻咽喉 | 12篇 |
儿科学 | 36篇 |
妇产科学 | 38篇 |
基础医学 | 301篇 |
口腔科学 | 22篇 |
临床医学 | 198篇 |
内科学 | 350篇 |
皮肤病学 | 38篇 |
神经病学 | 162篇 |
特种医学 | 38篇 |
外科学 | 139篇 |
综合类 | 17篇 |
一般理论 | 2篇 |
预防医学 | 194篇 |
眼科学 | 39篇 |
药学 | 173篇 |
中国医学 | 9篇 |
肿瘤学 | 163篇 |
出版年
2024年 | 3篇 |
2023年 | 18篇 |
2022年 | 57篇 |
2021年 | 92篇 |
2020年 | 47篇 |
2019年 | 52篇 |
2018年 | 90篇 |
2017年 | 62篇 |
2016年 | 75篇 |
2015年 | 73篇 |
2014年 | 76篇 |
2013年 | 145篇 |
2012年 | 177篇 |
2011年 | 178篇 |
2010年 | 68篇 |
2009年 | 87篇 |
2008年 | 105篇 |
2007年 | 97篇 |
2006年 | 80篇 |
2005年 | 72篇 |
2004年 | 75篇 |
2003年 | 71篇 |
2002年 | 57篇 |
2001年 | 10篇 |
2000年 | 2篇 |
1999年 | 9篇 |
1998年 | 10篇 |
1997年 | 7篇 |
1996年 | 3篇 |
1995年 | 3篇 |
1994年 | 3篇 |
1993年 | 4篇 |
1992年 | 2篇 |
1991年 | 2篇 |
1990年 | 1篇 |
1989年 | 3篇 |
1984年 | 2篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 2篇 |
1979年 | 1篇 |
1978年 | 3篇 |
1977年 | 1篇 |
1975年 | 2篇 |
1970年 | 1篇 |
1934年 | 1篇 |
排序方式: 共有1931条查询结果,搜索用时 738 毫秒
21.
Veronika Leichtfried Gabriel Putzer Dieter Perkhofer Wolfgang Schobersberger Arnulf Benzer 《Sleep & breathing》2011,15(3):503-512
Purpose
It has been shown that shift work constitutes a great health hazard, particularly when chronodisruption is involved. Anesthetists are used to working for a certain number of 24-h shifts every month. The work-related lack of sleep in combination with light exposure is suspected to alter melatonin courses. The main aim of the present study was to analyze circadian melatonin profiles before, during, and after a 24-h shift in anesthetists and medical students (controls). Furthermore, we evaluated possible differences in melatonin profiles between the groups. Interactions between specific parameters were calculated. 相似文献22.
Katerina Chudejova Veronika Rotova Anna Skalova Matej Medvecky Vaclava Adamkova Costas C. Papagiannitsis Jaroslav Hrabak 《Diagnostic microbiology and infectious disease》2018,90(2):148-150
ST252 Enterobacter cloacae, producing GES-5 carbapenemase, was isolated in a Czech hospital. blaGES-5 was part of a novel class 1 integron, In1406, which also included a new allele of the aadA15 gene cassette. In1406 was located on a ColE2-like plasmid, pEcl-35771cz (6953 bp). 相似文献
23.
Lenka Sedlarikova Barbora Gromesova Veronika Kubaczkova Lenka Radova Jana Filipova Jiri Jarkovsky Lucie Brozova Roberta Velichova Martina Almasi Miroslav Penka Renata Bezdekova Martin Stork Zdenek Adam Ludek Pour Marta Krejci Petr Kuglík Roman Hajek Sabina Sevcikova 《European journal of haematology》2017,99(3):223-233
24.
Yasmeen Niazi Hauke Thomsen Bozena Smolkova Ludmila Vodickova Sona Vodenkova Michal Kroupa Veronika Vymetalkova Alena Kazimirova Magdalena Barancokova Katarina Volkovova Marta Staruchova Per Hoffmann Markus M. Nöthen Maria Dušinská Ludovit Musak Pavel Vodicka Kari Hemminki Asta Försti 《Environmental and molecular mutagenesis》2019,60(1):17-28
Chromosomal aberrations (CAs) in human peripheral blood lymphocytes (PBL) measured with the conventional cytogenetic assay have been used for human biomonitoring of genotoxic exposure for decades. CA frequency in peripheral blood is a marker of cancer susceptibility. Previous studies have shown associations between genetic variants in metabolic pathway, DNA repair and major mitotic checkpoint genes and CAs. We conducted a genome-wide association study on 576 individuals from the Czech Republic and Slovakia followed by a replication in two different sample sets of 482 (replication 1) and 1288 (replication 2) samples. To have a broad look at the genetic susceptibility associated with CA frequency, the sample sets composed of individuals either differentially exposed to smoking, occupational/environmental hazards, or they were untreated cancer patients. Phenotypes were divided into chromosome- and chromatid-type aberrations (CSAs and CTAs, respectively) and total chromosomal aberrations (CAtot). The arbitrary cutoff point between individuals with high and low CA frequency was 2% for CAtot and 1% for CSA and CTA. The data were analyzed using age, sex, occupation/cancer and smoking history as covariates. Altogether 11 loci reached the P-value of 10−5 in the GWAS. Replication 1 supported the association of rs1383997 (8q13.3) and rs2824215 (21q21.1) in CAtot and rs983889 (5p15.1) in CTA analysis. These loci were found to be associated with genes involved in mitosis, response to environmental and chemical factors and genes involved in syndromes linked to chromosomal abnormalities. Identification of new genetic variants for the frequency of CAs offers prediction tools for cancer risk in future. Environ. Mol. Mutagen. 60:17–28, 2019. © 2018 Wiley Periodicals, Inc. 相似文献
25.
26.
27.
28.
Vanessa M. Noriega Thomas J. Gardner Veronika Redmann Gerold Bongers Sergio A. Lira Domenico Tortorella 《Viruses》2014,6(3):1202-1218
Human cytomegalovirus (HCMV) encodes a number of viral proteins with homology to cellular G protein-coupled receptors (GPCRs). These viral GPCRs, including US27, US28, UL33, and UL78, have been ascribed numerous functions during infection, including activating diverse cellular pathways, binding to immunomodulatory chemokines, and impacting virus dissemination. To investigate the role of US28 during virus infection, two variants of the clinical isolate TB40/E were generated: TB40/E-US28YFP expressing a C-terminal yellow fluorescent protein tag, and TB40/E-FLAGYFP in which a FLAG-YFP cassette replaces the US28 coding region. The TB40/E-US28YFP protein localized as large perinuclear fluorescent structures at late times post-infection in fibroblasts, endothelial, and epithelial cells. Interestingly, US28YFP is a non-glycosylated membrane protein throughout the course of infection. US28 appears to impact cell-to-cell spread of virus, as the ΔUS28 virus (TB40/E-FLAGYFP) generated a log-greater yield of extracellular progeny whose spread could be significantly neutralized in fibroblasts. Most strikingly, in epithelial cells, where dissemination of virus occurs exclusively by the cell-to-cell route, TB40/E-FLAGYFP (ΔUS28) displayed a significant growth defect. The data demonstrates that HCMV US28 may contribute at a late stage of the viral life cycle to cell-to-cell dissemination of virus. 相似文献
29.
Targeting of heat shock protein 32 (Hsp32)/heme oxygenase-1 (HO-1) in leukemic cells in chronic myeloid leukemia: a novel approach to overcome resistance against imatinib 下载免费PDF全文
Mayerhofer M Gleixner KV Mayerhofer J Hoermann G Jaeger E Aichberger KJ Ott RG Greish K Nakamura H Derdak S Samorapoompichit P Pickl WF Sexl V Esterbauer H Schwarzinger I Sillaber C Maeda H Valent P 《Blood》2008,111(4):2200-2210
Resistance toward imatinib and other BCR/ABL tyrosine kinase inhibitors remains an increasing clinical problem in the treatment of advanced stages of chronic myeloid leukemia (CML). We recently have identified the heat shock protein 32 (Hsp32)/heme oxygenase-1 (HO-1) as a BCR/ABL-dependent survival molecule in CML cells. We here show that silencing Hsp32/HO-1 in CML cells by an siRNA approach results in induction of apoptosis. Moreover, targeting Hsp32/HO-1 by either pegylated zinc protoporphyrine (PEG-ZnPP) or styrene maleic acid-micelle-encapsulated ZnPP (SMA-ZnPP) resulted in growth inhibition of BCR/ABL-transformed cells. The effects of PEG-ZnPP and SMA-ZnPP were demonstrable in Ba/F3 cells carrying various imatinib-resistant mutants of BCR/ABL, including the T315I mutant, which exhibits resistance against all clinically available BCR/ABL tyrosine kinase inhibitors. Growth-inhibitory effects of PEG-ZnPP and SMA-ZnPP also were observed in the CML-derived human cell lines K562 and KU812 as well as in primary leukemic cells obtained from patients with freshly diagnosed CML or imatinib-resistant CML. Finally, Hsp32/HO-1-targeting compounds were found to synergize with either imatinib or nilotinib in producing growth inhibition in imatinib-resistant K562 cells and in Ba/F3 cells harboring the T315I mutant of BCR/ABL. In summary, these data show that HO-1 is a promising novel target in imatinib-resistant CML. 相似文献