全文获取类型
收费全文 | 20221篇 |
免费 | 916篇 |
国内免费 | 96篇 |
专业分类
耳鼻咽喉 | 192篇 |
儿科学 | 349篇 |
妇产科学 | 393篇 |
基础医学 | 2717篇 |
口腔科学 | 393篇 |
临床医学 | 1221篇 |
内科学 | 4810篇 |
皮肤病学 | 338篇 |
神经病学 | 1896篇 |
特种医学 | 830篇 |
外科学 | 3159篇 |
综合类 | 89篇 |
一般理论 | 5篇 |
预防医学 | 656篇 |
眼科学 | 392篇 |
药学 | 1246篇 |
中国医学 | 24篇 |
肿瘤学 | 2523篇 |
出版年
2022年 | 167篇 |
2021年 | 351篇 |
2020年 | 195篇 |
2019年 | 253篇 |
2018年 | 316篇 |
2017年 | 269篇 |
2016年 | 297篇 |
2015年 | 324篇 |
2014年 | 419篇 |
2013年 | 539篇 |
2012年 | 880篇 |
2011年 | 881篇 |
2010年 | 486篇 |
2009年 | 434篇 |
2008年 | 757篇 |
2007年 | 878篇 |
2006年 | 862篇 |
2005年 | 803篇 |
2004年 | 773篇 |
2003年 | 785篇 |
2002年 | 858篇 |
2001年 | 656篇 |
2000年 | 725篇 |
1999年 | 668篇 |
1998年 | 260篇 |
1997年 | 203篇 |
1996年 | 200篇 |
1995年 | 189篇 |
1994年 | 193篇 |
1993年 | 176篇 |
1992年 | 632篇 |
1991年 | 549篇 |
1990年 | 545篇 |
1989年 | 555篇 |
1988年 | 496篇 |
1987年 | 480篇 |
1986年 | 433篇 |
1985年 | 436篇 |
1984年 | 245篇 |
1983年 | 206篇 |
1982年 | 92篇 |
1979年 | 230篇 |
1978年 | 128篇 |
1977年 | 100篇 |
1975年 | 99篇 |
1974年 | 98篇 |
1973年 | 97篇 |
1970年 | 100篇 |
1969年 | 114篇 |
1968年 | 94篇 |
排序方式: 共有10000条查询结果,搜索用时 661 毫秒
921.
Nishida N Yamashita S Mimori K Sudo T Tanaka F Shibata K Yamamoto H Ishii H Doki Y Mori M 《Annals of surgical oncology》2012,19(9):3065-3071
Purpose
Recent evidence has shown that altered patterns of microRNA (miRNA) expression correlate with various human cancers. We investigated the clinical significance of miR-10b and its involvement in chemotherapeutic resistance to 5-fluorouracil (5-FU), which is a key component of common chemotherapy regimens in colorectal cancer.Methods
Quantitative RT-PCR was used to evaluate the clinicopathologic significance of miR-10b expression in 88 colorectal cancer cases. We also investigated the chemotherapeutic sensitivity to 5-FU in miR-10b-overexpressing colorectal cancer cells. To explore the mechanism of chemoresistance in miR-10b transfected cells, we examined whether miR-10b inhibits the pro-apoptotic BH3-only Bcl-2 family member BIM(BCL2L11), a key mediator of chemotherapy-induced cell death.Results
High level miR-10b expression was found to be significantly associated with high incidence of lymphatic invasion (P?=?0.0257) and poor prognosis (P?=?0.0057). Multivariate analysis indicated that high miR-10b expression is an independent prognostic factor for survival. In vitro studies revealed that miR-10b directly inhibits pro-apoptotic BIM, and the overexpression of miR-10b confers chemoresistance in colorectal cancer cells to 5-FU.Conclusions
MiR-10b is a novel prognostic marker in colorectal cancer. Moreover, the expression of miR-10b is a potential indicator of chemosensitivity to the common 5-FU-based chemotherapy regimen. 相似文献922.
923.
Atsushi Takeno MD Ichiro Takemasa MD Shigeto Seno PhD Makoto Yamasaki MD Masaaki Motoori MD Hiroshi Miyata MD Kiyokazu Nakajima MD Shuji Takiguchi MD Yoshiyuki Fujiwara MD Toshiro Nishida MD Toshitsugu Okayama PhD Kenichi Matsubara PhD Yoichi Takenaka PhD Hideo Matsuda PhD Morito Monden MD Masaki Mori MD Yuichiro Doki MD 《Annals of surgical oncology》2010,17(4):1033-1042
Background
Peritoneal relapse is the most common pattern of tumor progression in advanced gastric cancer. Clinicopathological findings are sometimes inadequate for predicting peritoneal relapse. The aim of this study was to identify patients at high risk of peritoneal relapse in a prospective study based on molecular prediction.Methods
RNA samples from 141 primary gastric cancer tissues after curative surgery were profiled using oligonucleotide microarrays covering 30,000 human probes. Firstly, we constructed a molecular prediction system and validated its robustness and prognostic validity by 500 times multiple validation by repeated random sampling in a retrospective set of 56 (38 relapse-free and 18 peritoneal-relapse) patients. Secondly, we applied this prediction to 85 patients of the prospective set to assess predictive accuracy and prognostic validity.Results
In the retrospective phase, repeated random validation yielded ~68% predictive accuracy and a 22-gene expression profile associated with peritoneal relapse was identified. The prediction system identified patients with poor prognosis. In the prospective phase, the molecular prediction yielded 76.9% overall accuracy. Kaplan–Meier analysis of peritoneal-relapse-free survival showed a significant difference between the “good signature group” and “poor signature group” (log-rank p = 0.0017). Multivariate analysis by Cox regression hazards model identified the molecular prediction as the only independent prognostic factor for peritoneal relapse.Conclusions
Gene expression profile inherent to primary gastric cancer tissues can be useful in prospective prediction of peritoneal relapse after curative surgery, potentially allowing individualized postoperative management to improve the prognosis of patients with advanced gastric cancer. 相似文献924.
925.
926.
Nosocomial BK Polyomavirus Infection Causing Hemorrhagic Cystitis Among Patients With Hematological Malignancies After Hematopoietic Stem Cell Transplantation
下载免费PDF全文
![点击此处可从《American journal of transplantation》网站下载免费的PDF全文](/ch/ext_images/free.gif)
J. Kato T. Mori T. Suzuki M. Ito T. C. Li M. Sakurai Y. Yamane R. Yamazaki Y. Koda T. Toyama N. Hasegawa S. Okamoto 《American journal of transplantation》2017,17(9):2428-2433
BK polyomavirus (BKPyV) is recognized as a pathogen that causes diseases such as hemorrhagic cystitis and nephritis after allogeneic hematopoietic stem cell transplantation (HSCT) or renal transplantation. BKPyV‐associated disease is thought to occur through reactivation under immunosuppression. However, the possibility of its nosocomial transmission and the clinical significance of such transmission have not been elucidated. During a 6‐month period, nine adult patients (median age: 47 years) who had hematological disorders and who were treated with HSCT (n = 7) or chemotherapy (n = 2) in a single hematology department developed hemorrhagic cystitis due to BKPyV infection. The polymerase chain reaction products of BKPyV DNA obtained from each patient were sequenced. Of the nine patients, six had subtype I, 2 had subtype IV, and 1 had subtype II or III. In the alignment of sequences, four and two of the six subtype I strains were completely homologous (100%). These results strongly suggest that BKPyV has the potential to cause nosocomial infection within a medical facility, especially among recipients of HSCT. Further studies are clearly warranted to elucidate the route(s) of BKPyV transmission in order to establish optimal infection control. 相似文献
927.
928.
Yoshiyuki Kuwabara Masahiro Kimura Akira Mitsui Hideaki Ishiguro Keisuke Tomoda Yoichiro Mori Ryo Ogawa Koshiro Harata Takeyasu Katada Yoshitaka Fujii 《Surgery today》2009,39(9):800-802
A segment of the transverse colon can be used for gastric reconstruction after a total gastrectomy. This report presents the
case of a 68-year-old woman with primary adenocarcinoma of the colon in a segment used for reconstruction after a total gastrectomy.
The interposed colon developed colon carcinoma 9 years after the gastric reconstruction. The possibility of a primary carcinoma
arising in a gastric colon interposition must be considered when employing the transverse colon as a gastric substitute. 相似文献
929.
Tomoko Sonoda Junichi Takada Kousuke Iba Sumiyo Asakura Toshihiko Yamashita Mitsuru Mori 《Journal of orthopaedic research》2012,30(10):1529-1534
We hypothesized that environmental factors might affect the relationship between genetic predisposition and the risk of bone mineral density (BMD) loss. Cases were 114 Japanese women with a confirmed diagnosis of postmenopausal osteoporosis and controls were 171 general Japanese women. Genetic risk of SNPs in the estrogen receptors was analyzed by a case–control study. The interaction between gene and environmental factors for osteoporosis were assessed by a case‐only design. Significant increases in osteoporosis risk were observed with minor alleles of rs2077647 located in the first exon and rs2234693 located in the first intron of estrogen receptor α (ESRα). Haplotype CC at these risk SNPs was strongly associated with osteoporosis risk (odds ratio [OR] = 3.15, 95% confidence interval [CI] = 1.83–5.41). There was a statistically significant interaction between haplotype CC and alcohol drinking; moderate alcohol consumption decreased genetic risk of osteoporosis (OR = 0.22, 95%CI = 0.05–0.83). © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:1529–1534, 2012 相似文献
930.
Hiroaki Nomori Kazunori Iwatani Hironori Kobayashi Atsushi Mori Shouichi Yoshioka 《Annals of thoracic and cardiovascular surgery》2006,12(2):83-88
To reduce or omit a mediastinal lymph node dissection in the patients with clinical stage I non-small cell lung cancer (NSCLC), several authors examined the prevalence of metastatic sites of lymph nodes. Because lymphatic drainage usually heads for the upper mediastinum in upper lobe cancer and for the lower mediastinum in lower lobe cancer, upper and lower mediastinal lymph node dissection could be reduced in lung cancers of lower lobe and upper lobe. By using sentinel node (SN) navigation surgery, it is possible to omit mediastinal lymph node dissection. Radiological findings are also useful to determine reduction of mediastinal lymph node dissection. In clinical stage Ia adenocarcinomas that show ground glass opacity (GGO) findings on computed tomography (CT) or negative for fluorodeoxyglucose accumulation on positron emission tomography (PET), mediastinal lymph node dissection can be omitted, because these types of adenocarcinomas rarely metastasize to the lymph nodes. By using these procedures, mediastinal lymph node dissection can be reduced or omitted with little risk of local recurrence. 相似文献